UniProtKB - Q86UW9 (DTX2_HUMAN)
Probable E3 ubiquitin-protein ligase DTX2
DTX2
Functioni
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 412 – 473 | RING-typePROSITE-ProRule annotationAdd BLAST | 62 |
GO - Molecular functioni
- ubiquitin protein ligase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- Notch signaling pathway Source: GO_Central
- protein ubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Notch signaling pathway, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q86UW9 |
Reactomei | R-HSA-2122948, Activated NOTCH1 Transmits Signal to the Nucleus |
SignaLinki | Q86UW9 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable E3 ubiquitin-protein ligase DTX2 (EC:2.3.2.27By similarity)Alternative name(s): Protein deltex-2 Short name: Deltex2 Short name: hDTX2 RING finger protein 58 RING-type E3 ubiquitin transferase DTX2Curated |
Gene namesi | Name:DTX2 Synonyms:KIAA1528, RNF58 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15973, DTX2 |
MIMi | 613141, gene |
neXtProti | NX_Q86UW9 |
VEuPathDBi | HostDB:ENSG00000091073 |
Subcellular locationi
Nucleus
- nuclear membrane Source: HPA
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 113878 |
OpenTargetsi | ENSG00000091073 |
PharmGKBi | PA27515 |
Miscellaneous databases
Pharosi | Q86UW9, Tbio |
Genetic variation databases
BioMutai | DTX2 |
DMDMi | 68067880 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219083 | 1 – 622 | Probable E3 ubiquitin-protein ligase DTX2Add BLAST | 622 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 213 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 215 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 233 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 249 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 256 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 360 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q86UW9 |
jPOSTi | Q86UW9 |
MassIVEi | Q86UW9 |
MaxQBi | Q86UW9 |
PaxDbi | Q86UW9 |
PeptideAtlasi | Q86UW9 |
PRIDEi | Q86UW9 |
ProteomicsDBi | 69923 [Q86UW9-1] 69924 [Q86UW9-2] |
PTM databases
iPTMneti | Q86UW9 |
PhosphoSitePlusi | Q86UW9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000091073, Expressed in lower esophagus mucosa and 162 other tissues |
ExpressionAtlasi | Q86UW9, baseline and differential |
Genevisiblei | Q86UW9, HS |
Organism-specific databases
HPAi | ENSG00000091073, Tissue enhanced (esophagus) |
Interactioni
Subunit structurei
Homodimer. May form a heterodimer with other members of the Deltex family.
Interacts with NOTCH1.
By similarityBinary interactionsi
Q86UW9
Protein-protein interaction databases
BioGRIDi | 125266, 153 interactors |
IntActi | Q86UW9, 126 interactors |
MINTi | Q86UW9 |
STRINGi | 9606.ENSP00000322885 |
Miscellaneous databases
RNActi | Q86UW9, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q86UW9 |
SMRi | Q86UW9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 97 | WWE 1PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 98 – 174 | WWE 2PROSITE-ProRule annotationAdd BLAST | 77 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 249 – 324 | DisorderedSequence analysisAdd BLAST | 76 | |
Regioni | 355 – 393 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 255 – 319 | Polar residuesSequence analysisAdd BLAST | 65 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 412 – 473 | RING-typePROSITE-ProRule annotationAdd BLAST | 62 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QQ9M, Eukaryota |
GeneTreei | ENSGT00940000157641 |
HOGENOMi | CLU_030422_4_0_1 |
InParanoidi | Q86UW9 |
OMAi | DAPEEDC |
OrthoDBi | 600021at2759 |
PhylomeDBi | Q86UW9 |
Family and domain databases
CDDi | cd09633, Deltex_C, 1 hit |
Gene3Di | 3.30.390.130, 1 hit 3.30.40.10, 1 hit 3.30.720.50, 2 hits |
InterProi | View protein in InterPro IPR039396, Deltex_C IPR039399, Deltex_C_sf IPR039398, Deltex_fam IPR004170, WWE-dom IPR018123, WWE-dom_subgr IPR037197, WWE_dom_sf IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR12622, PTHR12622, 1 hit |
Pfami | View protein in Pfam PF18102, DTC, 1 hit PF02825, WWE, 2 hits PF00097, zf-C3HC4, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00678, WWE, 2 hits |
SUPFAMi | SSF117839, SSF117839, 2 hits |
PROSITEi | View protein in PROSITE PS50918, WWE, 2 hits PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMAPSPSLV QVYTSPAAVA VWEWQDGLGT WHPYSATVCS FIEQQFVQQK
60 70 80 90 100
GQRFGLGSLA HSIPLGQADP SLAPYIIDLP SWTQFRQDTG TMRAVRRHLF
110 120 130 140 150
PQHSAPGRGV VWEWLSDDGS WTAYEASVCD YLEQQVARGN QLVDLAPLGY
160 170 180 190 200
NYTVNYTTHT QTNKTSSFCR SVRRQAGPPY PVTTIIAPPG HTGVACSCHQ
210 220 230 240 250
CLSGSRTGPV SGRYRHSMTN LPAYPVPQHP PHRTASVFGT HQAFAPYNKP
260 270 280 290 300
SLSGARSAPR LNTTNAWGAA PPSLGSQPLY RSSLSHLGPQ HLPPGSSTSG
310 320 330 340 350
AVSASLPSGP SSSPGSVPAT VPMQMPKPSR VQQALAGMTS VLMSAIGLPV
360 370 380 390 400
CLSRAPQPTS PPASRLASKS HGSVKRLRKM SVKGATPKPE PEPEQVIKNY
410 420 430 440 450
TEELKVPPDE DCIICMEKLS TASGYSDVTD SKAIGSLAVG HLTKCSHAFH
460 470 480 490 500
LLCLLAMYCN GNKDGSLQCP SCKTIYGEKT GTQPQGKMEV LRFQMSLPGH
510 520 530 540 550
EDCGTILIVY SIPHGIQGPE HPNPGKPFTA RGFPRQCYLP DNAQGRKVLE
560 570 580 590 600
LLKVAWKRRL IFTVGTSSTT GETDTVVWNE IHHKTEMDRN ITGHGYPDPN
610 620
YLQNVLAELA AQGVTEDCLE QQ
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7ET89 | E7ET89_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 531 | Annotation score: | ||
C9JEH4 | C9JEH4_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 108 | Annotation score: | ||
C9JII1 | C9JII1_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 140 | Annotation score: | ||
C9JF39 | C9JF39_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 62 | Annotation score: | ||
C9JVC8 | C9JVC8_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 65 | Annotation score: | ||
C9JY61 | C9JY61_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 89 | Annotation score: | ||
Q75MM6 | Q75MM6_HUMAN | E3 ubiquitin-protein ligase | DTX2 | 212 | Annotation score: | ||
H7C3B8 | H7C3B8_HUMAN | Probable E3 ubiquitin-protein ligas... | DTX2 | 87 | Annotation score: | ||
C9IYX1 | C9IYX1_HUMAN | Probable E3 ubiquitin-protein ligas... | DTX2 | 14 | Annotation score: | ||
C9JS11 | C9JS11_HUMAN | Probable E3 ubiquitin-protein ligas... | DTX2 | 28 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_016920 | 94 | A → T. Corresponds to variant dbSNP:rs2462312Ensembl. | 1 | |
Natural variantiVAR_016921 | 384 | G → E3 PublicationsCorresponds to variant dbSNP:rs1638152Ensembl. | 1 | |
Natural variantiVAR_016922 | 421 | T → A3 PublicationsCorresponds to variant dbSNP:rs6979487Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008350 | 337 – 383 | Missing in isoform 2. CuratedAdd BLAST | 47 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000324432.9; ENSP00000322885.5; ENSG00000091073.20 ENST00000413936.6; ENSP00000390218.2; ENSG00000091073.20 ENST00000430490.7; ENSP00000411986.2; ENSG00000091073.20 ENST00000446820.6; ENSP00000392545.2; ENSG00000091073.20 [Q86UW9-2] |
GeneIDi | 113878 |
KEGGi | hsa:113878 |
MANE-Selecti | ENST00000430490.7; ENSP00000411986.2; NM_001102594.3; NP_001096064.1 |
UCSCi | uc003uff.5, human [Q86UW9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6IR0 | NMR | - | A | 399-474 | [»] | |
6Y22 | X-ray | 2.07 | A | 390-622 | [»] | |
6Y2X | X-ray | 1.77 | A/B | 390-622 | [»] | |
6Y3J | X-ray | 2.60 | A | 390-622 | [»] | |
AlphaFoldDBi | Q86UW9 | |||||
SMRi | Q86UW9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125266, 153 interactors |
IntActi | Q86UW9, 126 interactors |
MINTi | Q86UW9 |
STRINGi | 9606.ENSP00000322885 |
PTM databases
iPTMneti | Q86UW9 |
PhosphoSitePlusi | Q86UW9 |
Genetic variation databases
BioMutai | DTX2 |
DMDMi | 68067880 |
Proteomic databases
EPDi | Q86UW9 |
jPOSTi | Q86UW9 |
MassIVEi | Q86UW9 |
MaxQBi | Q86UW9 |
PaxDbi | Q86UW9 |
PeptideAtlasi | Q86UW9 |
PRIDEi | Q86UW9 |
ProteomicsDBi | 69923 [Q86UW9-1] 69924 [Q86UW9-2] |
Protocols and materials databases
Antibodypediai | 35109, 195 antibodies from 31 providers |
DNASUi | 113878 |
Genome annotation databases
Ensembli | ENST00000324432.9; ENSP00000322885.5; ENSG00000091073.20 ENST00000413936.6; ENSP00000390218.2; ENSG00000091073.20 ENST00000430490.7; ENSP00000411986.2; ENSG00000091073.20 ENST00000446820.6; ENSP00000392545.2; ENSG00000091073.20 [Q86UW9-2] |
GeneIDi | 113878 |
KEGGi | hsa:113878 |
MANE-Selecti | ENST00000430490.7; ENSP00000411986.2; NM_001102594.3; NP_001096064.1 |
UCSCi | uc003uff.5, human [Q86UW9-1] |
Organism-specific databases
CTDi | 113878 |
DisGeNETi | 113878 |
GeneCardsi | DTX2 |
HGNCi | HGNC:15973, DTX2 |
HPAi | ENSG00000091073, Tissue enhanced (esophagus) |
MIMi | 613141, gene |
neXtProti | NX_Q86UW9 |
OpenTargetsi | ENSG00000091073 |
PharmGKBi | PA27515 |
VEuPathDBi | HostDB:ENSG00000091073 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQ9M, Eukaryota |
GeneTreei | ENSGT00940000157641 |
HOGENOMi | CLU_030422_4_0_1 |
InParanoidi | Q86UW9 |
OMAi | DAPEEDC |
OrthoDBi | 600021at2759 |
PhylomeDBi | Q86UW9 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q86UW9 |
Reactomei | R-HSA-2122948, Activated NOTCH1 Transmits Signal to the Nucleus |
SignaLinki | Q86UW9 |
Miscellaneous databases
BioGRID-ORCSi | 113878, 34 hits in 1116 CRISPR screens |
ChiTaRSi | DTX2, human |
GeneWikii | DTX2 |
GenomeRNAii | 113878 |
Pharosi | Q86UW9, Tbio |
PROi | PR:Q86UW9 |
RNActi | Q86UW9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000091073, Expressed in lower esophagus mucosa and 162 other tissues |
ExpressionAtlasi | Q86UW9, baseline and differential |
Genevisiblei | Q86UW9, HS |
Family and domain databases
CDDi | cd09633, Deltex_C, 1 hit |
Gene3Di | 3.30.390.130, 1 hit 3.30.40.10, 1 hit 3.30.720.50, 2 hits |
InterProi | View protein in InterPro IPR039396, Deltex_C IPR039399, Deltex_C_sf IPR039398, Deltex_fam IPR004170, WWE-dom IPR018123, WWE-dom_subgr IPR037197, WWE_dom_sf IPR018957, Znf_C3HC4_RING-type IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR12622, PTHR12622, 1 hit |
Pfami | View protein in Pfam PF18102, DTC, 1 hit PF02825, WWE, 2 hits PF00097, zf-C3HC4, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00678, WWE, 2 hits |
SUPFAMi | SSF117839, SSF117839, 2 hits |
PROSITEi | View protein in PROSITE PS50918, WWE, 2 hits PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DTX2_HUMAN | |
Accessioni | Q86UW9Primary (citable) accession number: Q86UW9 Secondary accession number(s): Q4ZH49 Q9P200 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | June 21, 2005 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families