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Protein

NEDD4-binding protein 2

Gene

N4BP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair or recombination.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi447 – 454ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: MGI
  • ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: MGI
  • endonuclease activity Source: MGI

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD4-binding protein 2 (EC:3.-.-.-)
Short name:
N4BP2
Alternative name(s):
BCL-3-binding protein
Gene namesi
Name:N4BP2
Synonyms:B3BP, KIAA1413
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000078177.13
HGNCiHGNC:29851 N4BP2
neXtProtiNX_Q86UW6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55728
OpenTargetsiENSG00000078177
PharmGKBiPA162396580

Polymorphism and mutation databases

BioMutaiN4BP2
DMDMi145559498

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966811 – 1770NEDD4-binding protein 2Add BLAST1770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei906PhosphoserineCombined sources1
Modified residuei1210PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated; this targets the protein for degradation by the proteasome.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86UW6
MaxQBiQ86UW6
PaxDbiQ86UW6
PeptideAtlasiQ86UW6
PRIDEiQ86UW6
ProteomicsDBi69917
69918 [Q86UW6-2]

PTM databases

iPTMnetiQ86UW6
PhosphoSitePlusiQ86UW6

Expressioni

Gene expression databases

BgeeiENSG00000078177
CleanExiHS_N4BP2
ExpressionAtlasiQ86UW6 baseline and differential
GenevisibleiQ86UW6 HS

Organism-specific databases

HPAiHPA036770
HPA042607

Interactioni

Subunit structurei

Binds NEDD4 (By similarity). Binds BCL3 and CREBBP.By similarity

Protein-protein interaction databases

BioGridi120848, 13 interactors
CORUMiQ86UW6
IntActiQ86UW6, 7 interactors
STRINGi9606.ENSP00000261435

Structurei

Secondary structure

11770
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1669 – 1685Combined sources17
Helixi1686 – 1688Combined sources3
Beta strandi1690 – 1692Combined sources3
Helixi1698 – 1719Combined sources22
Beta strandi1724 – 1728Combined sources5
Beta strandi1734 – 1736Combined sources3
Helixi1742 – 1752Combined sources11
Beta strandi1757 – 1761Combined sources5
Beta strandi1764 – 1768Combined sources5

3D structure databases

ProteinModelPortaliQ86UW6
SMRiQ86UW6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86UW6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 89CUEPROSITE-ProRule annotationAdd BLAST44
Domaini1691 – 1770SmrPROSITE-ProRule annotationAdd BLAST80

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili90 – 177Sequence analysisAdd BLAST88
Coiled coili218 – 259Sequence analysisAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi328 – 368Pro-richAdd BLAST41

Domaini

The Smr domain has nicking endonuclease activity, but no significant double strand cleavage or exonuclease activity.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2401 Eukaryota
ENOG41101K4 LUCA
GeneTreeiENSGT00510000047015
HOGENOMiHOG000113717
HOVERGENiHBG101251
InParanoidiQ86UW6
KOiK15720
OMAiDTWWKFK
OrthoDBiEOG091G00G9
PhylomeDBiQ86UW6
TreeFamiTF327016

Family and domain databases

InterProiView protein in InterPro
IPR003892 CUE
IPR013899 DUF1771
IPR027417 P-loop_NTPase
IPR002625 Smr_dom
IPR036063 Smr_dom_sf
IPR009060 UBA-like_sf
PfamiView protein in Pfam
PF08590 DUF1771, 1 hit
PF01713 Smr, 1 hit
SMARTiView protein in SMART
SM01162 DUF1771, 1 hit
SM00463 SMR, 1 hit
SUPFAMiSSF160443 SSF160443, 1 hit
SSF46934 SSF46934, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51140 CUE, 1 hit
PS50828 SMR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL
60 70 80 90 100
FTSISEIFSD LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ
110 120 130 140 150
SFVASENQVG AAESKIMEKR PEEESEDSKM DSFLDMQLTE DLDSLIQNAF
160 170 180 190 200
EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI NPDSSNMTPI FSTQNMNLNG
210 220 230 240 250
ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP EDSLLSSSLN
260 270 280 290 300
VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL
310 320 330 340 350
DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL
360 370 380 390 400
PLLLPPPPPP PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS
410 420 430 440 450
VISHTSPTKV WRNKDGTSAY QVQETPVSQV VRKKTSYVGL VLVLLRGLPG
460 470 480 490 500
SGKSFLARTL QEDNPSGVIL STDDYFYING QYQFDVKYLG EAHEWNQNRA
510 520 530 540 550
KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR EPDTWWKFKP
560 570 580 590 600
KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS
610 620 630 640 650
CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV
660 670 680 690 700
AYLSNADLNK RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN
710 720 730 740 750
EQIEMVAVKG YSKTDTDSSM ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS
760 770 780 790 800
SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP RHELSNFVGD WPVDKTIGQR
810 820 830 840 850
TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ RGSPHESVED
860 870 880 890 900
GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM
910 920 930 940 950
PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE
960 970 980 990 1000
MLLSEMTCES QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE
1010 1020 1030 1040 1050
QMPKRDPGKE VGMCTQTEPQ DFALLWKIEK NKISISDSIK VLTGRLDGFK
1060 1070 1080 1090 1100
PKVFNINTKS DVQEAIPYRV MYDKSTFVEE SELTSADESE NLNILCKLFG
1110 1120 1130 1140 1150
SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN FQKSCDGSQI
1160 1170 1180 1190 1200
GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN
1210 1220 1230 1240 1250
SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF
1260 1270 1280 1290 1300
PGILKATTPK DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN
1310 1320 1330 1340 1350
EEIYFTDSLE IKRNENFPKD YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA
1360 1370 1380 1390 1400
GVSGEDKTEI LNPTPAMAKS LTIDCLELAL PPELAFQLNE LFGPVGIDSG
1410 1420 1430 1440 1450
SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK FMQDPSLVGH
1460 1470 1480 1490 1500
TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE
1510 1520 1530 1540 1550
IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY
1560 1570 1580 1590 1600
SLEHTVQFLN CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK
1610 1620 1630 1640 1650
ETEETPSELS FQDFEYPDYD DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN
1660 1670 1680 1690 1700
VATFYAQQGT LHEQKMKEAN HLAAIEIFEK VNASLLPQNV LDLHGLHVDE
1710 1720 1730 1740 1750
ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA RIKPAVIKYL
1760 1770
ISHSFRFSEI KPGCLKVMLK
Length:1,770
Mass (Da):198,801
Last modified:April 17, 2007 - v2
Checksum:i9A49FAB19C1BD56D
GO
Isoform 2 (identifier: Q86UW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1596-1612: Missing.

Note: No experimental confirmation available.
Show »
Length:1,753
Mass (Da):196,828
Checksum:iB391A9B622FFCBFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118E → K in AAI26467 (PubMed:15489334).Curated1
Sequence conflicti428S → F in BAA91748 (PubMed:14702039).Curated1
Sequence conflicti660K → Q in AAP22172 (PubMed:12730195).Curated1
Sequence conflicti778K → E in BAA92651 (PubMed:10718198).Curated1
Sequence conflicti1353S → R in AAI26467 (PubMed:15489334).Curated1
Sequence conflicti1353S → R in BAA92651 (PubMed:10718198).Curated1
Sequence conflicti1524L → V in AAP22172 (PubMed:12730195).Curated1
Sequence conflicti1591S → F in AAP22172 (PubMed:12730195).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051215101S → I. Corresponds to variant dbSNP:rs17511668Ensembl.1
Natural variantiVAR_051216196M → V. Corresponds to variant dbSNP:rs10014170Ensembl.1
Natural variantiVAR_035474283P → A in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_051217611D → N3 PublicationsCorresponds to variant dbSNP:rs794001Ensembl.1
Natural variantiVAR_0512181587T → A. Corresponds to variant dbSNP:rs2271395Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0097211596 – 1612Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY267013 mRNA Translation: AAP22172.1
BC126466 mRNA Translation: AAI26467.1
AK001542 mRNA Translation: BAA91748.1
AB037834 mRNA Translation: BAA92651.1
CCDSiCCDS3457.1 [Q86UW6-1]
RefSeqiNP_001305288.1, NM_001318359.1
NP_060647.2, NM_018177.5 [Q86UW6-1]
XP_011512020.1, XM_011513718.2 [Q86UW6-1]
XP_016863887.1, XM_017008398.1 [Q86UW6-1]
UniGeneiHs.391463
Hs.736026

Genome annotation databases

EnsembliENST00000261435; ENSP00000261435; ENSG00000078177 [Q86UW6-1]
GeneIDi55728
KEGGihsa:55728
UCSCiuc003guy.5 human [Q86UW6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiN4BP2_HUMAN
AccessioniPrimary (citable) accession number: Q86UW6
Secondary accession number(s): A0AVR3, Q9NVK2, Q9P2D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: April 17, 2007
Last modified: June 20, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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