UniProtKB - Q86UK7 (ZN598_HUMAN)
E3 ubiquitin-protein ligase ZNF598
ZNF598
Functioni
E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation (PubMed:28065601, PubMed:28132843).
Required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3 (PubMed:28065601, PubMed:28132843).
Stalling precludes synthesis of a long poly-lysine tail and initiates the RQC pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843).
Also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:22751931).
3 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 69 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 187 – 210 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ribosome binding Source: UniProtKB
- RNA binding Source: UniProtKB
- ubiquitin protein ligase activity Source: UniProtKB
GO - Biological processi
- protein monoubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- rescue of stalled ribosome Source: UniProtKB
- ribosome-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Translation regulation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q86UK7 |
SignaLinki | Q86UK7 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase ZNF598Curated (EC:2.3.2.272 Publications)Alternative name(s): Zinc finger protein 598Imported |
Gene namesi | Name:ZNF598Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28079, ZNF598 |
MIMi | 617508, gene |
neXtProti | NX_Q86UK7 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | C → A: Abolishes E3 ubiquitin-protein ligase activity, leading to enhanced readthrough on the poly(A)-stall sequences. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 90850 |
PharmGKBi | PA134944505 |
Miscellaneous databases
Pharosi | Q86UK7, Tbio |
Genetic variation databases
BioMutai | ZNF598 |
DMDMi | 74727495 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000250568 | 1 – 904 | E3 ubiquitin-protein ligase ZNF598Add BLAST | 904 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 306 | PhosphotyrosineCombined sources | 1 | ||
Modified residuei | 437 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: Q86UK7-2) | |||||
Modified residuei | 428 | PhosphoserineCombined sources | 1 | ||
Isoform 3 (identifier: Q86UK7-3) | |||||
Modified residuei | 431 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q86UK7 |
jPOSTi | Q86UK7 |
MassIVEi | Q86UK7 |
MaxQBi | Q86UK7 |
PaxDbi | Q86UK7 |
PeptideAtlasi | Q86UK7 |
PRIDEi | Q86UK7 |
ProteomicsDBi | 69826 [Q86UK7-1] 69827 [Q86UK7-2] 69828 [Q86UK7-3] 69829 [Q86UK7-4] |
PTM databases
GlyGeni | Q86UK7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q86UK7 |
PhosphoSitePlusi | Q86UK7 |
SwissPalmi | Q86UK7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000167962, Expressed in adenohypophysis and 167 other tissues |
Genevisiblei | Q86UK7, HS |
Interactioni
Subunit structurei
Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598 (PubMed:22751931).
1 PublicationProtein-protein interaction databases
BioGRIDi | 124771, 417 interactors |
IntActi | Q86UK7, 27 interactors |
MINTi | Q86UK7 |
STRINGi | 9606.ENSP00000411409 |
Miscellaneous databases
RNActi | Q86UK7, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 312 – 469 | DisorderedSequence analysisAdd BLAST | 158 | |
Regioni | 490 – 656 | DisorderedSequence analysisAdd BLAST | 167 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 332 – 347 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 359 – 392 | Basic and acidic residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 399 – 415 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 446 – 461 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 495 – 533 | Polar residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 565 – 582 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 600 – 614 | Pro residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 29 – 69 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 187 – 210 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2231, Eukaryota |
HOGENOMi | CLU_015828_0_0_1 |
InParanoidi | Q86UK7 |
OrthoDBi | 1003372at2759 |
PhylomeDBi | Q86UK7 |
TreeFami | TF316196 |
Family and domain databases
CDDi | cd16615, RING-HC_ZNF598, 1 hit |
InterProi | View protein in InterPro IPR041888, RING-HC_ZNF598/Hel2 IPR044288, ZNF598/Hel2 IPR013087, Znf_C2H2_type IPR001841, Znf_RING |
PANTHERi | PTHR22938, PTHR22938, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS00028, ZINC_FINGER_C2H2_1, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAGGAEGR RAALEAAAAA APERGGGSCV LCCGDLEATA LGRCDHPVCY
60 70 80 90 100
RCSTKMRVLC EQRYCAVCRE ELRQVVFGKK LPAFATIPIH QLQHEKKYDI
110 120 130 140 150
YFADGKVYAL YRQLLQHECP RCPELPPFSL FGDLEQHMRR QHELFCCRLC
160 170 180 190 200
LQHLQIFTYE RKWYSRKDLA RHRMQGDPDD TSHRGHPLCK FCDERYLDND
210 220 230 240 250
ELLKHLRRDH YFCHFCDSDG AQDYYSDYAY LREHFREKHF LCEEGRCSTE
260 270 280 290 300
QFTHAFRTEI DLKAHRTACH SRSRAEARQN RHIDLQFSYA PRHSRRNEGV
310 320 330 340 350
VGGEDYEEVD RYSRQGRVAR AGTRGAQQSR RGSWRYKREE EDREVAAAVR
360 370 380 390 400
ASVAAQQQEE ARRSEDQEEG GRPKKEEAAA RGPEDPRGPR RSPRTQGEGP
410 420 430 440 450
GPKETSTNGP VSQEAFSVTG PAAPGCVGVP GALPPPSPKL KDEDFPSLSA
460 470 480 490 500
STSSSCSTAA TPGPVGLALP YAIPARGRSA FQEEDFPALV SSVPKPGTAP
510 520 530 540 550
TSLVSAWNSS SSSKKVAQPP LSAQATGSGQ PTRKAGKGSR GGRKGGPPFT
560 570 580 590 600
QEEEEDGGPA LQELLSTRPT GSVSSTLGLA SIQPSKVGKK KKVGSEKPGT
610 620 630 640 650
TLPQPPPATC PPGALQAPEA PASRAEGPVA VVVNGHTEGP APARSAPKEP
660 670 680 690 700
PGLPRPLGSF PCPTPQEDFP ALGGPCPPRM PPPPGFSAVV LLKGTPPPPP
710 720 730 740 750
PGLVPPISKP PPGFSGLLPS PHPACVPSPA TTTTTKAPRL LPAPRAYLVP
760 770 780 790 800
ENFRERNLQL IQSIRDFLQS DEARFSEFKS HSGEFRQGLI SAAQYYKSCR
810 820 830 840 850
DLLGENFQKV FNELLVLLPD TAKQQELLSA HTDFCNREKP LSTKSKKNKK
860 870 880 890 900
SAWQATTQQA GLDCRVCPTC QQVLAHGDAS SHQALHAARD DDFPSLQAIA
RIIT
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BPG6 | H3BPG6_HUMAN | RING-type E3 ubiquitin transferase | ZNF598 | 904 | Annotation score: | ||
H3BQQ2 | H3BQQ2_HUMAN | E3 ubiquitin-protein ligase ZNF598 | ZNF598 | 849 | Annotation score: | ||
H3BR87 | H3BR87_HUMAN | E3 ubiquitin-protein ligase ZNF598 | ZNF598 | 203 | Annotation score: | ||
H3BPF3 | H3BPF3_HUMAN | E3 ubiquitin-protein ligase ZNF598 | ZNF598 | 90 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_034470 | 453 | S → Y2 PublicationsCorresponds to variant dbSNP:rs11556528Ensembl. | 1 | |
Natural variantiVAR_059818 | 608 | A → T2 PublicationsCorresponds to variant dbSNP:rs11248905Ensembl. | 1 | |
Natural variantiVAR_052147 | 637 | T → M2 PublicationsCorresponds to variant dbSNP:rs2286469Ensembl. | 1 | |
Natural variantiVAR_034471 | 725 | C → S1 PublicationCorresponds to variant dbSNP:rs2286468Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020660 | 1 – 397 | Missing in isoform 4. 1 PublicationAdd BLAST | 397 | |
Alternative sequenceiVSP_020661 | 335 – 337 | Missing in isoform 2. 1 Publication | 3 | |
Alternative sequenceiVSP_020662 | 398 – 431 | EGPGP…VGVPG → MVGGCGQPQVGAGRAGMEPR GLIAVDQLCFPAPS in isoform 4. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_020663 | 424 – 429 | Missing in isoform 2 and isoform 3. 2 Publications | 6 | |
Alternative sequenceiVSP_020664 | 551 | Q → QE in isoform 2 and isoform 4. 2 Publications | 1 | |
Alternative sequenceiVSP_020665 | 738 – 904 | Missing in isoform 4. 1 PublicationAdd BLAST | 167 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK024487 mRNA Translation: BAB15777.1 Different initiation. BC010990 mRNA Translation: AAH10990.2 BC041015 mRNA Translation: AAH41015.1 BC050477 mRNA Translation: AAH50477.1 AL834428 mRNA Translation: CAD39089.1 |
RefSeqi | NP_835461.2, NM_178167.3 |
Genome annotation databases
GeneIDi | 90850 |
KEGGi | hsa:90850 |
UCSCi | uc002cof.3, human [Q86UK7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK024487 mRNA Translation: BAB15777.1 Different initiation. BC010990 mRNA Translation: AAH10990.2 BC041015 mRNA Translation: AAH41015.1 BC050477 mRNA Translation: AAH50477.1 AL834428 mRNA Translation: CAD39089.1 |
RefSeqi | NP_835461.2, NM_178167.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 124771, 417 interactors |
IntActi | Q86UK7, 27 interactors |
MINTi | Q86UK7 |
STRINGi | 9606.ENSP00000411409 |
PTM databases
GlyGeni | Q86UK7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q86UK7 |
PhosphoSitePlusi | Q86UK7 |
SwissPalmi | Q86UK7 |
Genetic variation databases
BioMutai | ZNF598 |
DMDMi | 74727495 |
Proteomic databases
EPDi | Q86UK7 |
jPOSTi | Q86UK7 |
MassIVEi | Q86UK7 |
MaxQBi | Q86UK7 |
PaxDbi | Q86UK7 |
PeptideAtlasi | Q86UK7 |
PRIDEi | Q86UK7 |
ProteomicsDBi | 69826 [Q86UK7-1] 69827 [Q86UK7-2] 69828 [Q86UK7-3] 69829 [Q86UK7-4] |
Protocols and materials databases
DNASUi | 90850 |
Genome annotation databases
GeneIDi | 90850 |
KEGGi | hsa:90850 |
UCSCi | uc002cof.3, human [Q86UK7-1] |
Organism-specific databases
CTDi | 90850 |
DisGeNETi | 90850 |
GeneCardsi | ZNF598 |
HGNCi | HGNC:28079, ZNF598 |
MIMi | 617508, gene |
neXtProti | NX_Q86UK7 |
PharmGKBi | PA134944505 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2231, Eukaryota |
HOGENOMi | CLU_015828_0_0_1 |
InParanoidi | Q86UK7 |
OrthoDBi | 1003372at2759 |
PhylomeDBi | Q86UK7 |
TreeFami | TF316196 |
Enzyme and pathway databases
PathwayCommonsi | Q86UK7 |
SignaLinki | Q86UK7 |
Miscellaneous databases
BioGRID-ORCSi | 90850, 28 hits in 305 CRISPR screens |
ChiTaRSi | ZNF598, human |
GenomeRNAii | 90850 |
Pharosi | Q86UK7, Tbio |
PROi | PR:Q86UK7 |
RNActi | Q86UK7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167962, Expressed in adenohypophysis and 167 other tissues |
Genevisiblei | Q86UK7, HS |
Family and domain databases
CDDi | cd16615, RING-HC_ZNF598, 1 hit |
InterProi | View protein in InterPro IPR041888, RING-HC_ZNF598/Hel2 IPR044288, ZNF598/Hel2 IPR013087, Znf_C2H2_type IPR001841, Znf_RING |
PANTHERi | PTHR22938, PTHR22938, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS00028, ZINC_FINGER_C2H2_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZN598_HUMAN | |
Accessioni | Q86UK7Primary (citable) accession number: Q86UK7 Secondary accession number(s): Q8IW49 Q9H7J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families