UniProtKB - Q86UF1 (TSN33_HUMAN)
Protein
Tetraspanin-33
Gene
TSPAN33
Organism
Homo sapiens (Human)
Status
Functioni
Plays an important role in normal erythropoiesis (By similarity). It has a role in the differentiation of erythroid progenitors (By similarity). Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862, PubMed:30463011). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862). Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed:30463011).By similarity2 Publications
GO - Molecular functioni
- enzyme binding Source: UniProtKB
GO - Biological processi
- cellular protein metabolic process Source: Reactome
- pore complex assembly Source: UniProtKB
- protein localization to plasma membrane Source: UniProtKB
- protein maturation Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q86UF1 |
Reactomei | R-HSA-977225, Amyloid fiber formation |
SIGNORi | Q86UF1 |
Protein family/group databases
TCDBi | 8.A.40.1.11, the tetraspanin (tetraspanin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Tetraspanin-33Short name: Tspan-33 Alternative name(s): Penumbra Short name: hPen Proerythroblast new membrane |
Gene namesi | Name:TSPAN33 Synonyms:PEN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28743, TSPAN33 |
MIMi | 610120, gene |
neXtProti | NX_Q86UF1 |
VEuPathDBi | HostDB:ENSG00000158457.5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Curated
Other locations
- adherens junction 1 Publication
- Cytoplasm 1 Publication
Note: Is localized to zonula adherens by PLEKHA7 by a PDZD11-dependent interaction.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
- tetraspanin-enriched microdomain Source: Ensembl
Other locations
- adherens junction Source: UniProtKB-SubCell
- cell surface Source: Ensembl
- pore complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 64 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 65 – 85 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 86 – 96 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 97 – 117 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 235 | ExtracellularSequence analysisAdd BLAST | 118 | |
Transmembranei | 236 – 256 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 257 – 283 | CytoplasmicSequence analysisAdd BLAST | 27 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 340348 |
OpenTargetsi | ENSG00000158457 |
PharmGKBi | PA142670690 |
Miscellaneous databases
Pharosi | Q86UF1, Tbio |
Genetic variation databases
BioMutai | TSPAN33 |
DMDMi | 74727485 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282922 | 1 – 283 | Tetraspanin-33Add BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 172 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q86UF1 |
jPOSTi | Q86UF1 |
MassIVEi | Q86UF1 |
MaxQBi | Q86UF1 |
PaxDbi | Q86UF1 |
PeptideAtlasi | Q86UF1 |
PRIDEi | Q86UF1 |
ProteomicsDBi | 69814 |
PTM databases
GlyGeni | Q86UF1, 1 site |
iPTMneti | Q86UF1 |
PhosphoSitePlusi | Q86UF1 |
SwissPalmi | Q86UF1 |
Expressioni
Tissue specificityi
Predominantly expressed in erythroblasts.1 Publication
Gene expression databases
Bgeei | ENSG00000158457, Expressed in kidney epithelium and 210 other tissues |
Genevisiblei | Q86UF1, HS |
Organism-specific databases
HPAi | ENSG00000158457, Tissue enhanced (kidney) |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (PubMed:17158226).
Interacts (via extracellular domain) with ADAM10 (via extracellular domain) (PubMed:26686862, PubMed:30463011).
Interacts (via cytoplasmic domain) with PLEKHA7 (via WW domains); the interaction is dependent on PDZD11 being bound to PLEKHA7 and facilitates the docking of ADAM10 to zonula adherens (PubMed:30463011).
3 PublicationsBinary interactionsi
Hide detailsQ86UF1
GO - Molecular functioni
- enzyme binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 131040, 31 interactors |
IntActi | Q86UF1, 33 interactors |
MINTi | Q86UF1 |
STRINGi | 9606.ENSP00000483872 |
Miscellaneous databases
RNActi | Q86UF1, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the tetraspanin (TM4SF) family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000159484 |
HOGENOMi | CLU_055524_0_3_1 |
InParanoidi | Q86UF1 |
OMAi | IFFLLEM |
OrthoDBi | 1416189at2759 |
PhylomeDBi | Q86UF1 |
TreeFami | TF313002 |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
i Sequence
Sequence statusi: Complete.
Q86UF1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARRPRAPAA SGEEFSFVSP LVKYLLFFFN MLFWVISMVM VAVGVYARLM
60 70 80 90 100
KHAEAALACL AVDPAILLIV VGVLMFLLTF CGCIGSLREN ICLLQTFSLC
110 120 130 140 150
LTAVFLLQLA AGILGFVFSD KARGKVSEII NNAIVHYRDD LDLQNLIDFG
160 170 180 190 200
QKKFSCCGGI SYKDWSQNMY FNCSEDNPSR ERCSVPYSCC LPTPDQAVIN
210 220 230 240 250
TMCGQGMQAF DYLEASKVIY TNGCIDKLVN WIHSNLFLLG GVALGLAIPQ
260 270 280
LVGILLSQIL VNQIKDQIKL QLYNQQHRAD PWY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC044244 mRNA Translation: AAH44244.1 AY236849 mRNA Translation: AAO91940.1 AF276891 mRNA Translation: AAQ14314.1 |
CCDSi | CCDS5810.1 |
RefSeqi | NP_848657.1, NM_178562.4 |
Genome annotation databases
Ensembli | ENST00000486685; ENSP00000483872; ENSG00000158457 |
GeneIDi | 340348 |
KEGGi | hsa:340348 |
UCSCi | uc033ahb.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC044244 mRNA Translation: AAH44244.1 AY236849 mRNA Translation: AAO91940.1 AF276891 mRNA Translation: AAQ14314.1 |
CCDSi | CCDS5810.1 |
RefSeqi | NP_848657.1, NM_178562.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 131040, 31 interactors |
IntActi | Q86UF1, 33 interactors |
MINTi | Q86UF1 |
STRINGi | 9606.ENSP00000483872 |
Protein family/group databases
TCDBi | 8.A.40.1.11, the tetraspanin (tetraspanin) family |
PTM databases
GlyGeni | Q86UF1, 1 site |
iPTMneti | Q86UF1 |
PhosphoSitePlusi | Q86UF1 |
SwissPalmi | Q86UF1 |
Genetic variation databases
BioMutai | TSPAN33 |
DMDMi | 74727485 |
Proteomic databases
EPDi | Q86UF1 |
jPOSTi | Q86UF1 |
MassIVEi | Q86UF1 |
MaxQBi | Q86UF1 |
PaxDbi | Q86UF1 |
PeptideAtlasi | Q86UF1 |
PRIDEi | Q86UF1 |
ProteomicsDBi | 69814 |
Protocols and materials databases
Antibodypediai | 17866, 198 antibodies |
DNASUi | 340348 |
Genome annotation databases
Ensembli | ENST00000486685; ENSP00000483872; ENSG00000158457 |
GeneIDi | 340348 |
KEGGi | hsa:340348 |
UCSCi | uc033ahb.2, human |
Organism-specific databases
CTDi | 340348 |
DisGeNETi | 340348 |
GeneCardsi | TSPAN33 |
HGNCi | HGNC:28743, TSPAN33 |
HPAi | ENSG00000158457, Tissue enhanced (kidney) |
MIMi | 610120, gene |
neXtProti | NX_Q86UF1 |
OpenTargetsi | ENSG00000158457 |
PharmGKBi | PA142670690 |
VEuPathDBi | HostDB:ENSG00000158457.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000159484 |
HOGENOMi | CLU_055524_0_3_1 |
InParanoidi | Q86UF1 |
OMAi | IFFLLEM |
OrthoDBi | 1416189at2759 |
PhylomeDBi | Q86UF1 |
TreeFami | TF313002 |
Enzyme and pathway databases
PathwayCommonsi | Q86UF1 |
Reactomei | R-HSA-977225, Amyloid fiber formation |
SIGNORi | Q86UF1 |
Miscellaneous databases
BioGRID-ORCSi | 340348, 8 hits in 870 CRISPR screens |
GenomeRNAii | 340348 |
Pharosi | Q86UF1, Tbio |
PROi | PR:Q86UF1 |
RNActi | Q86UF1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158457, Expressed in kidney epithelium and 210 other tissues |
Genevisiblei | Q86UF1, HS |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSN33_HUMAN | |
Accessioni | Q86UF1Primary (citable) accession number: Q86UF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families