UniProtKB - Q86UD5 (SL9B2_HUMAN)
Protein
Sodium/hydrogen exchanger 9B2
Gene
SLC9B2
Organism
Homo sapiens (Human)
Status
Functioni
Na+/H+ antiporter that extrudes Na+ or Li+ in exchange for external protons across the membrane (PubMed:18000046, PubMed:28154142, PubMed:22948142, PubMed:18508966). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (By similarity). Involved in sperm motility and fertility (By similarity). It is controversial whether SLC9B2 plays a role in osteoclast differentiation or not (By similarity).By similarity4 Publications
Miscellaneous
Inhibited by phloretin but not by the classical SLC9A-inhibitor amiloride (PubMed:18000046).1 Publication
Kineticsi
- KM=33 mM for Na+1 Publication
- KM=67 mM for Li+1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 278 | Important for cation transport1 Publication | 1 | |
Sitei | 279 | Important for cation transport1 Publication | 1 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- lithium:proton antiporter activity Source: UniProtKB
- monovalent cation:proton antiporter activity Source: Reactome
- sodium:proton antiporter activity Source: UniProtKB
GO - Biological processi
- clathrin-dependent endocytosis Source: Ensembl
- flagellated sperm motility Source: UniProtKB
- ion transmembrane transport Source: Reactome
- monovalent inorganic cation transport Source: GO_Central
- positive regulation of osteoclast development Source: Ensembl
- regulation of insulin secretion involved in cellular response to glucose stimulus Source: Ensembl
- sodium ion transport Source: UniProtKB
Keywordsi
Biological process | Antiport, Hydrogen ion transport, Ion transport, Sodium transport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
PathwayCommonsi | Q86UD5 |
Reactomei | R-HSA-2672351, Stimuli-sensing channels |
Protein family/group databases
TCDBi | 2.A.36.2.2, the monovalent cation:proton antiporter-1 (cpa1) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/hydrogen exchanger 9B2Alternative name(s): Na(+)/H(+) exchanger NHA2 Na(+)/H(+) exchanger-like domain-containing protein 2 Short name: NHE domain-containing protein 2 Sodium/hydrogen exchanger-like domain-containing protein 2 Solute carrier family 9 subfamily B member 2 |
Gene namesi | Name:SLC9B2 Synonyms:NHA2, NHEDC2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25143, SLC9B2 |
MIMi | 611789, gene |
neXtProti | NX_Q86UD5 |
VEuPathDBi | HostDB:ENSG00000164038.14 |
Subcellular locationi
Endosome
- Endosome membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- flagellum membrane By similarity; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Apical cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Mitochondrion
- Mitochondrion membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- synaptic vesicle membrane By similarity; Multi-pass membrane protein By similarity
Note: Strong colocalization with LAMP1 and TCIRG1 in osteoclasts. In beta-cells colocalizes with RAB4A and SYP. Localizes to the basolateral membrane of polarized osteoclasts.By similarity
Endosome
- endosome membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial inner membrane Source: Reactome
- mitochondrial membrane Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- basolateral plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
- sperm principal piece Source: UniProtKB
- synaptic vesicle membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 87 – 107 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 136 – 156 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 235 – 255 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 266 – 286 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 307 – 327 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 341 – 361 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 363 – 383 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 388 – 408 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 425 – 445 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 493 – 513 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cilium, Cytoplasmic vesicle, Endosome, Flagellum, Membrane, Mitochondrion, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 278 – 279 | DD → CC: Loss of ion transport activity; Does not rescue insulin secretion defect induced by knockdown of SLC9B2 in Min6 cells. 2 Publications | 2 | |
Mutagenesisi | 432 | R → K or H: Loss of ion transport activity;. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 133308 |
OpenTargetsi | ENSG00000164038 |
PharmGKBi | PA162397515 |
Miscellaneous databases
Pharosi | Q86UD5, Tbio |
Genetic variation databases
BioMutai | SLC9B2 |
DMDMi | 121944426 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000331270 | 1 – 537 | Sodium/hydrogen exchanger 9B2Add BLAST | 537 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 49 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q86UD5 |
jPOSTi | Q86UD5 |
MassIVEi | Q86UD5 |
MaxQBi | Q86UD5 |
PaxDbi | Q86UD5 |
PeptideAtlasi | Q86UD5 |
PRIDEi | Q86UD5 |
ProteomicsDBi | 69803 [Q86UD5-1] 69804 [Q86UD5-2] |
PTM databases
iPTMneti | Q86UD5 |
PhosphoSitePlusi | Q86UD5 |
Expressioni
Tissue specificityi
Widely expressed (PubMed:18508966). High levels detected in the distal tubules of the kidney nephron (PubMed:18508966). Detected in red blood cells (at protein level) (PubMed:18000046, PubMed:18508966).2 Publications
Gene expression databases
Bgeei | ENSG00000164038, Expressed in tibia and 165 other tissues |
ExpressionAtlasi | Q86UD5, baseline and differential |
Genevisiblei | Q86UD5, HS |
Organism-specific databases
HPAi | ENSG00000164038, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Homodimer (PubMed:28071645).
1 PublicationBinary interactionsi
Q86UD5
With | #Exp. | IntAct |
---|---|---|
PGRMC2 [O15173] | 3 | EBI-9916342,EBI-1050125 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 126354, 5 interactors |
IntActi | Q86UD5, 4 interactors |
STRINGi | 9606.ENSP00000378265 |
Miscellaneous databases
RNActi | Q86UD5, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3826, Eukaryota |
GeneTreei | ENSGT00390000013285 |
HOGENOMi | CLU_018415_4_1_1 |
InParanoidi | Q86UD5 |
OMAi | PAILMCF |
PhylomeDBi | Q86UD5 |
TreeFami | TF319087 |
Family and domain databases
Gene3Di | 1.20.1530.20, 1 hit |
InterProi | View protein in InterPro IPR006153, Cation/H_exchanger IPR038770, Na+/solute_symporter_sf IPR037072, SLC9B2 |
PANTHERi | PTHR31102:SF14, PTHR31102:SF14, 1 hit |
Pfami | View protein in Pfam PF00999, Na_H_Exchanger, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q86UD5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDEDKRITY EDSEPSTGMN YTPSMHQEAQ EETVMKLKGI DANEPTEGSI
60 70 80 90 100
LLKSSEKKLQ ETPTEANHVQ RLRQMLACPP HGLLDRVITN VTIIVLLWAV
110 120 130 140 150
VWSITGSECL PGGNLFGIII LFYCAIIGGK LLGLIKLPTL PPLPSLLGML
160 170 180 190 200
LAGFLIRNIP VINDNVQIKH KWSSSLRSIA LSIILVRAGL GLDSKALKKL
210 220 230 240 250
KGVCVRLSMG PCIVEACTSA LLAHYLLGLP WQWGFILGFV LGAVSPAVVV
260 270 280 290 300
PSMLLLQGGG YGVEKGVPTL LMAAGSFDDI LAITGFNTCL GIAFSTGSTV
310 320 330 340 350
FNVLRGVLEV VIGVATGSVL GFFIQYFPSR DQDKLVCKRT FLVLGLSVLA
360 370 380 390 400
VFSSVHFGFP GSGGLCTLVM AFLAGMGWTS EKAEVEKIIA VAWDIFQPLL
410 420 430 440 450
FGLIGAEVSI ASLRPETVGL CVATVGIAVL IRILTTFLMV CFAGFNLKEK
460 470 480 490 500
IFISFAWLPK ATVQAAIGSV ALDTARSHGE KQLEDYGMDV LTVAFLSILI
510 520 530
TAPIGSLLIG LLGPRLLQKV EHQNKDEEVQ GETSVQV
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7Z676 | B7Z676_HUMAN | Sodium/hydrogen exchanger 9B2 | SLC9B2 | 417 | Annotation score: | ||
E9PE63 | E9PE63_HUMAN | Sodium/hydrogen exchanger 9B2 | SLC9B2 | 480 | Annotation score: | ||
D6RGJ7 | D6RGJ7_HUMAN | Sodium/hydrogen exchanger 9B2 | SLC9B2 | 143 | Annotation score: | ||
A0A0C4DGB3 | A0A0C4DGB3_HUMAN | Sodium/hydrogen exchanger 9B2 | SLC9B2 | 354 | Annotation score: | ||
D6R9P2 | D6R9P2_HUMAN | Sodium/hydrogen exchanger 9B2 | SLC9B2 | 112 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_042751 | 159 | I → T. Corresponds to variant dbSNP:rs7672710Ensembl. | 1 | |
Natural variantiVAR_042752 | 161 | V → A. Corresponds to variant dbSNP:rs7672707Ensembl. | 1 | |
Natural variantiVAR_042753 | 357 | F → C. Corresponds to variant dbSNP:rs2276976Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_033152 | 420 – 502 | LCVAT…ILITA → SADSITGNFGTERPKLLGPP STQLRFHFFHIQLST in isoform 2. 1 PublicationAdd BLAST | 83 | |
Alternative sequenceiVSP_033153 | 503 – 537 | Missing in isoform 2. 1 PublicationAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172823 mRNA Translation: BAD18790.1 AC097485 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX06159.1 BC009732 mRNA Translation: AAH09732.1 BC047447 mRNA Translation: AAH47447.2 |
CCDSi | CCDS3662.1 [Q86UD5-1] |
RefSeqi | NP_001287685.1, NM_001300756.1 NP_849155.2, NM_178833.5 [Q86UD5-1] XP_006714148.1, XM_006714085.2 |
Genome annotation databases
Ensembli | ENST00000362026; ENSP00000354574; ENSG00000164038 [Q86UD5-1] ENST00000394785; ENSP00000378265; ENSG00000164038 [Q86UD5-1] |
GeneIDi | 133308 |
KEGGi | hsa:133308 |
UCSCi | uc003hwx.5, human [Q86UD5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172823 mRNA Translation: BAD18790.1 AC097485 Genomic DNA No translation available. CH471057 Genomic DNA Translation: EAX06159.1 BC009732 mRNA Translation: AAH09732.1 BC047447 mRNA Translation: AAH47447.2 |
CCDSi | CCDS3662.1 [Q86UD5-1] |
RefSeqi | NP_001287685.1, NM_001300756.1 NP_849155.2, NM_178833.5 [Q86UD5-1] XP_006714148.1, XM_006714085.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 126354, 5 interactors |
IntActi | Q86UD5, 4 interactors |
STRINGi | 9606.ENSP00000378265 |
Protein family/group databases
TCDBi | 2.A.36.2.2, the monovalent cation:proton antiporter-1 (cpa1) family |
PTM databases
iPTMneti | Q86UD5 |
PhosphoSitePlusi | Q86UD5 |
Genetic variation databases
BioMutai | SLC9B2 |
DMDMi | 121944426 |
Proteomic databases
EPDi | Q86UD5 |
jPOSTi | Q86UD5 |
MassIVEi | Q86UD5 |
MaxQBi | Q86UD5 |
PaxDbi | Q86UD5 |
PeptideAtlasi | Q86UD5 |
PRIDEi | Q86UD5 |
ProteomicsDBi | 69803 [Q86UD5-1] 69804 [Q86UD5-2] |
Protocols and materials databases
Antibodypediai | 26081, 158 antibodies |
Genome annotation databases
Ensembli | ENST00000362026; ENSP00000354574; ENSG00000164038 [Q86UD5-1] ENST00000394785; ENSP00000378265; ENSG00000164038 [Q86UD5-1] |
GeneIDi | 133308 |
KEGGi | hsa:133308 |
UCSCi | uc003hwx.5, human [Q86UD5-1] |
Organism-specific databases
CTDi | 133308 |
DisGeNETi | 133308 |
GeneCardsi | SLC9B2 |
HGNCi | HGNC:25143, SLC9B2 |
HPAi | ENSG00000164038, Tissue enhanced (liver) |
MIMi | 611789, gene |
neXtProti | NX_Q86UD5 |
OpenTargetsi | ENSG00000164038 |
PharmGKBi | PA162397515 |
VEuPathDBi | HostDB:ENSG00000164038.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3826, Eukaryota |
GeneTreei | ENSGT00390000013285 |
HOGENOMi | CLU_018415_4_1_1 |
InParanoidi | Q86UD5 |
OMAi | PAILMCF |
PhylomeDBi | Q86UD5 |
TreeFami | TF319087 |
Enzyme and pathway databases
PathwayCommonsi | Q86UD5 |
Reactomei | R-HSA-2672351, Stimuli-sensing channels |
Miscellaneous databases
BioGRID-ORCSi | 133308, 4 hits in 874 CRISPR screens |
ChiTaRSi | SLC9B2, human |
GenomeRNAii | 133308 |
Pharosi | Q86UD5, Tbio |
PROi | PR:Q86UD5 |
RNActi | Q86UD5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164038, Expressed in tibia and 165 other tissues |
ExpressionAtlasi | Q86UD5, baseline and differential |
Genevisiblei | Q86UD5, HS |
Family and domain databases
Gene3Di | 1.20.1530.20, 1 hit |
InterProi | View protein in InterPro IPR006153, Cation/H_exchanger IPR038770, Na+/solute_symporter_sf IPR037072, SLC9B2 |
PANTHERi | PTHR31102:SF14, PTHR31102:SF14, 1 hit |
Pfami | View protein in Pfam PF00999, Na_H_Exchanger, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SL9B2_HUMAN | |
Accessioni | Q86UD5Primary (citable) accession number: Q86UD5 Secondary accession number(s): B5ME52, Q6ZMD8, Q96D95 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | August 30, 2005 | |
Last modified: | February 10, 2021 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants