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Protein

E3 ubiquitin-protein ligase MARCH3

Gene

MARCH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 123RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processEndocytosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH3 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 3
Membrane-associated RING-CH protein III
Short name:
MARCH-III
RING finger protein 173
RING-type E3 ubiquitin transferase MARCH3Curated
Gene namesi
Name:MARCH3
Synonyms:RNF173
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000173926.5
HGNCiHGNC:28728 MARCH3
MIMi613333 gene
neXtProtiNX_Q86UD3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi115123
OpenTargetsiENSG00000173926
PharmGKBiPA134901491

Polymorphism and mutation databases

DMDMi59798460

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559271 – 253E3 ubiquitin-protein ligase MARCH3Add BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei237PhosphoserineCombined sources1
Modified residuei243PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86UD3
PaxDbiQ86UD3
PRIDEiQ86UD3
ProteomicsDBi69801

PTM databases

iPTMnetiQ86UD3
PhosphoSitePlusiQ86UD3

Expressioni

Gene expression databases

BgeeiENSG00000173926
CleanExiHS_MARCH3
GenevisibleiQ86UD3 HS

Organism-specific databases

HPAiHPA043406

Interactioni

Subunit structurei

Interacts with MARCH2 and STX6.By similarity

Protein-protein interaction databases

BioGridi125416, 8 interactors
IntActiQ86UD3, 10 interactors
MINTiQ86UD3
STRINGi9606.ENSP00000309141

Structurei

3D structure databases

ProteinModelPortaliQ86UD3
SMRiQ86UD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 123RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609 Eukaryota
COG5183 LUCA
GeneTreeiENSGT00920000148985
HOGENOMiHOG000293394
HOVERGENiHBG052411
InParanoidiQ86UD3
KOiK10658
OMAiCELCHFK
OrthoDBiEOG091G14LW
PhylomeDBiQ86UD3
TreeFamiTF319557

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR033275 MARCH-like
IPR011016 Znf_RING-CH
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR23012 PTHR23012, 1 hit
PfamiView protein in Pfam
PF12906 RINGv, 1 hit
SMARTiView protein in SMART
SM00744 RINGv, 1 hit
PROSITEiView protein in PROSITE
PS51292 ZF_RING_CH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UD3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSRCSHLP EVLPDCTSSA APVVKTVEDC GSLVNGQPQY VMQVSAKDGQ
60 70 80 90 100
LLSTVVRTLA TQSPFNDRPM CRICHEGSSQ EDLLSPCECT GTLGTIHRSC
110 120 130 140 150
LEHWLSSSNT SYCELCHFRF AVERKPRPLV EWLRNPGPQH EKRTLFGDMV
160 170 180 190 200
CFLFITPLAT ISGWLCLRGA VDHLHFSSRL EAVGLIALTV ALFTIYLFWT
210 220 230 240 250
LVSFRYHCRL YNEWRRTNQR VILLIPKSVN VPSNQPSLLG LHSVKRNSKE

TVV
Length:253
Mass (Da):28,504
Last modified:June 1, 2003 - v1
Checksum:i6E090675CEBC8A88
GO
Isoform 2 (identifier: Q86UD3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-138: WLRNPGP → VSKWGTS
     139-253: Missing.

Note: No experimental confirmation available.
Show »
Length:138
Mass (Da):15,176
Checksum:iCEB724ACE5833B21
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05363968R → Q1 PublicationCorresponds to variant dbSNP:rs34821177Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055451132 – 138WLRNPGP → VSKWGTS in isoform 2. 1 Publication7
Alternative sequenceiVSP_055452139 – 253Missing in isoform 2. 1 PublicationAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK290129 mRNA Translation: BAF82818.1
AC004507 Genomic DNA No translation available.
AC005221 Genomic DNA No translation available.
AC093532 Genomic DNA No translation available.
AC137794 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62412.1
CH471062 Genomic DNA Translation: EAW62413.1
BC047569 mRNA Translation: AAH47569.1
BC146948 mRNA Translation: AAI46949.1
BC146964 mRNA Translation: AAI46965.1
CCDSiCCDS4141.1 [Q86UD3-1]
RefSeqiNP_848545.1, NM_178450.4 [Q86UD3-1]
UniGeneiHs.132441

Genome annotation databases

EnsembliENST00000308660; ENSP00000309141; ENSG00000173926 [Q86UD3-1]
ENST00000515241; ENSP00000421979; ENSG00000173926 [Q86UD3-2]
GeneIDi115123
KEGGihsa:115123
UCSCiuc003kuf.4 human [Q86UD3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMARH3_HUMAN
AccessioniPrimary (citable) accession number: Q86UD3
Secondary accession number(s): A8K264, B9EJE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2003
Last modified: July 18, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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