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Protein

RPA-interacting protein

Gene

RPAIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 212RIP-typeAdd BLAST76

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein-containing complex binding Source: MGI

GO - Biological processi

  • DNA-dependent DNA replication Source: MGI
  • DNA recombination Source: MGI
  • DNA repair Source: MGI
  • protein import into nucleus Source: MGI
  • response to UV Source: MGI

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RPA-interacting protein
Short name:
hRIP
Gene namesi
Name:RPAIN
Synonyms:RIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000129197.14
HGNCiHGNC:28641 RPAIN
MIMi617299 gene
neXtProtiNX_Q86UA6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114K → R: Abolishes sumoylation; when associated with R-121 and R-142. 1 Publication1
Mutagenesisi121K → R: Induces a strong decrease in sumoylation; when associated with N-103. Abolishes sumoylation; when associated with R-114 and R-142. 1 Publication1
Mutagenesisi142K → R: Abolishes sumoylation; when associated with R-114 and R-121. 1 Publication1

Organism-specific databases

DisGeNETi84268
OpenTargetsiENSG00000129197
PharmGKBiPA145007849

Polymorphism and mutation databases

BioMutaiRPAIN
DMDMi74727468

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762991 – 219RPA-interacting proteinAdd BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); in isoform 21 Publication

Post-translational modificationi

Isoform 2: sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues. Variant 'Lys-103' adds a sumoylation site and increases total sumoylation levels.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86UA6
PaxDbiQ86UA6
PeptideAtlasiQ86UA6
PRIDEiQ86UA6
ProteomicsDBi69786
69787 [Q86UA6-2]
69788 [Q86UA6-3]
69789 [Q86UA6-4]
69790 [Q86UA6-5]
69791 [Q86UA6-6]
69792 [Q86UA6-7]

PTM databases

iPTMnetiQ86UA6
PhosphoSitePlusiQ86UA6

Expressioni

Tissue specificityi

Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis, prostate, thymus and spleen.1 Publication

Gene expression databases

BgeeiENSG00000129197
CleanExiHS_RPAIN
ExpressionAtlasiQ86UA6 baseline and differential
GenevisibleiQ86UA6 HS

Organism-specific databases

HPAiHPA023924
HPA031526

Interactioni

Subunit structurei

Interacts with the RPA1 subunit of RPA complex.1 Publication

Protein-protein interaction databases

BioGridi123995, 10 interactors
IntActiQ86UA6, 7 interactors
STRINGi9606.ENSP00000385814

Structurei

3D structure databases

ProteinModelPortaliQ86UA6
SMRiQ86UA6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 180Mediates nuclear exportAdd BLAST17

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 212RIP-typeAdd BLAST76

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IX54 Eukaryota
ENOG4111K6G LUCA
GeneTreeiENSGT00390000006416
HOGENOMiHOG000251596
HOVERGENiHBG082800
InParanoidiQ86UA6
PhylomeDBiQ86UA6
TreeFamiTF326215

Family and domain databases

InterProiView protein in InterPro
IPR028156 RIP
IPR028159 RPA_interact_C_dom
IPR028155 RPA_interact_central
IPR028158 RPA_interact_N_dom
PANTHERiPTHR31742 PTHR31742, 1 hit
PfamiView protein in Pfam
PF14768 RPA_interact_C, 1 hit
PF14767 RPA_interact_M, 1 hit
PF14766 RPA_interact_N, 1 hit

Sequences (9)i

Sequence statusi: Complete.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UA6-1) [UniParc]FASTAAdd to basket
Also known as: Alpha, hRIPalpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS
60 70 80 90 100
GPGNSQNSFL VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE
110 120 130 140 150
LINQEQSIIS EYEKSLQFDE KCLSIMLAEW EANPLICPVC TKYNLRITSG
160 170 180 190 200
VVVCQCGLSI PSHSSELTEQ KLRACLEGSI NEHSAHCPHT PEFSVTGGTE
210
EKSSLLMSCL ACDTWAVIL
Note: Major isoform with isoform 2.
Length:219
Mass (Da):24,770
Last modified:March 28, 2018 - v2
Checksum:iBAF576893B8B1B23
GO
Isoform 2 (identifier: Q86UA6-2) [UniParc]FASTAAdd to basket
Also known as: Beta, hRIPbeta

The sequence of this isoform differs from the canonical sequence as follows:
     164-210: Missing.

Note: Major isoform with isoform 1.
Show »
Length:172
Mass (Da):19,712
Checksum:i0F3FF24C142EA4F4
GO
Isoform 3 (identifier: Q86UA6-3) [UniParc]FASTAAdd to basket
Also known as: Gamma2

The sequence of this isoform differs from the canonical sequence as follows:
     143-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. May be due to an intron retention.
Show »
Length:142
Mass (Da):16,511
Checksum:iB4DD41B3708D8E5F
GO
Isoform 4 (identifier: Q86UA6-4) [UniParc]FASTAAdd to basket
Also known as: Gamma1

The sequence of this isoform differs from the canonical sequence as follows:
     142-145: KYNL → NLLS
     146-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:145
Mass (Da):16,810
Checksum:iA7977963AD41B370
GO
Isoform 5 (identifier: Q86UA6-5) [UniParc]FASTAAdd to basket
Also known as: Delta2

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → VF
     107-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:106
Mass (Da):12,385
Checksum:i184280616713B68C
GO
Isoform 6 (identifier: Q86UA6-6) [UniParc]FASTAAdd to basket
Also known as: Delta3

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → GL
     107-219: Missing.

Show »
Length:106
Mass (Da):12,309
Checksum:i16B830616713B68C
GO
Isoform 7 (identifier: Q86UA6-7) [UniParc]FASTAAdd to basket
Also known as: Delta1, Delta 4

The sequence of this isoform differs from the canonical sequence as follows:
     105-107: EQS → GTT
     108-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:107
Mass (Da):12,398
Checksum:iA2803830616713B6
GO
Isoform 8 (identifier: Q86UA6-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-219: ACDTWAVIL → VSVSWDPLCGKRDLWLVLFPP

Note: No experimental confirmation available.
Show »
Length:231
Mass (Da):26,207
Checksum:i216DCFE77EF90199
GO
Isoform 9 (identifier: Q86UA6-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-219: YNLRITSGVV...LACDTWAVIL → PVILGL

Note: No experimental confirmation available.
Show »
Length:148
Mass (Da):17,103
Checksum:i0A6FE6F2628924DD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023947103N → K Common polymorphism; sumoylated and increases total protein sumoylation levels. 4 PublicationsCorresponds to variant dbSNP:rs12761Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016402105 – 107EQS → GTT in isoform 7. 2 Publications3
Alternative sequenceiVSP_016403105 – 106EQ → VF in isoform 5. 2 Publications2
Alternative sequenceiVSP_016404105 – 106EQ → GL in isoform 6. 3 Publications2
Alternative sequenceiVSP_016405107 – 219Missing in isoform 5 and isoform 6. 3 PublicationsAdd BLAST113
Alternative sequenceiVSP_016406108 – 219Missing in isoform 7. 2 PublicationsAdd BLAST112
Alternative sequenceiVSP_016407142 – 145KYNL → NLLS in isoform 4. 3 Publications4
Alternative sequenceiVSP_016408143 – 219Missing in isoform 3. 2 PublicationsAdd BLAST77
Alternative sequenceiVSP_046795143 – 219YNLRI…WAVIL → PVILGL in isoform 9. CuratedAdd BLAST77
Alternative sequenceiVSP_016409146 – 219Missing in isoform 4. 3 PublicationsAdd BLAST74
Alternative sequenceiVSP_016410164 – 210Missing in isoform 2. 2 PublicationsAdd BLAST47
Alternative sequenceiVSP_045356211 – 219ACDTWAVIL → VSVSWDPLCGKRDLWLVLFP P in isoform 8. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775314 mRNA Translation: AAX14368.1
AY775315 mRNA Translation: AAX14369.1
AY775316 mRNA Translation: AAX14370.1
AY775317 mRNA Translation: AAX14371.1
AY775318 mRNA Translation: AAX14372.1
AY775319 mRNA Translation: AAX14373.1
AY775320 mRNA Translation: AAX14374.1
AY775321 mRNA Translation: AAX14375.1
AY775323 mRNA Translation: AAX14377.1
AY680654 mRNA Translation: AAT80872.1
AY680655 mRNA Translation: AAT80873.1
AY680656 mRNA Translation: AAT80874.1
AY680657 mRNA Translation: AAT80875.1
AY680658 mRNA Translation: AAT80876.1
AY680659 mRNA Translation: AAT80877.1
AY680660 mRNA Translation: AAT80878.1
AK295394 mRNA Translation: BAG58348.1
AK300409 mRNA Translation: BAG62139.1
CH471108 Genomic DNA Translation: EAW90338.1
AC004148 Genomic DNA No translation available.
BC004451 mRNA Translation: AAH04451.1
BC046349 mRNA Translation: AAH46349.1
BC051849 mRNA Translation: AAH51849.1
CCDSiCCDS32536.1 [Q86UA6-1]
CCDS54075.1 [Q86UA6-8]
CCDS54076.1 [Q86UA6-2]
CCDS54077.1 [Q86UA6-9]
CCDS54079.1 [Q86UA6-6]
RefSeqiNP_001028174.2, NM_001033002.3 [Q86UA6-1]
NP_001153715.1, NM_001160243.1 [Q86UA6-8]
NP_001153716.1, NM_001160244.1 [Q86UA6-2]
NP_001153718.1, NM_001160246.1 [Q86UA6-6]
NP_001153738.1, NM_001160266.1 [Q86UA6-9]
UniGeneiHs.462086
Hs.555866

Genome annotation databases

EnsembliENST00000327154; ENSP00000315069; ENSG00000129197 [Q86UA6-9]
ENST00000381208; ENSP00000370605; ENSG00000129197 [Q86UA6-2]
ENST00000381209; ENSP00000370606; ENSG00000129197 [Q86UA6-1]
ENST00000405578; ENSP00000385814; ENSG00000129197 [Q86UA6-8]
ENST00000536255; ENSP00000439939; ENSG00000129197 [Q86UA6-6]
ENST00000539417; ENSP00000446453; ENSG00000129197 [Q86UA6-4]
ENST00000571558; ENSP00000458699; ENSG00000129197 [Q86UA6-7]
ENST00000573577; ENSP00000460162; ENSG00000129197 [Q86UA6-7]
ENST00000574003; ENSP00000467178; ENSG00000129197 [Q86UA6-5]
ENST00000575112; ENSP00000460354; ENSG00000129197 [Q86UA6-7]
ENST00000575599; ENSP00000458218; ENSG00000129197 [Q86UA6-5]
GeneIDi84268
KEGGihsa:84268
UCSCiuc002gbw.3 human [Q86UA6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRIP_HUMAN
AccessioniPrimary (citable) accession number: Q86UA6
Secondary accession number(s): A0A0B4J1T3
, B4DI36, B4DTX7, E9PDG9, E9PES3, J3KNH8, Q4G2Y0, Q4G2Y5, Q4G2Y8, Q6B4V9, Q6B4W0, Q6B4W1, Q6B4W4, Q86X49, Q9BT00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 28, 2018
Last modified: July 18, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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