UniProtKB - Q86U10 (LPP60_HUMAN)
Protein
60 kDa lysophospholipase
Gene
ASPG
Organism
Homo sapiens (Human)
Status
Functioni
Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities (By similarity). Can catalyze three types of transacylation reactions: (1) acyl transfer from 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to the sn-13 positions of glycerol and 2-acylglycerol (sn-1 to -13 transfer), (2) acyl transfer from 1-acyl-GPC to the sn-2 positions of 1-acyl-GPC, 1-acyl-sn-glycero-3-phosphoethanolamine (1-acyl-GPE), and other lysophospholipids (sn-1 to -2 transfer) and (3) acyl transfer from 2-acyl-GPC to the sn-1 position of 2-acyl-GPC and 2-acyl-GPE (sn-2 to -1 transfer) (By similarity). Mediates the synthesis of 1-arachidonoyl species of phospholipids by transferring the arachidonoyl residue from 2-arachidonoyl lysophospholipid to the sn-1 position of 2-acyl lysophospholipid (By similarity).By similarity
Catalytic activityi
- a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H+ + sn-glycerol 3-phosphocholineBy similarityEC:3.1.1.5By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.5.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H+By similarityEC:3.1.1.47By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + octadecanoate + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- a 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + H+ + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H2O = H+ + hexadecanoate + sn-glycero-3-phosphoethanolamineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z)-octadecenoate + H+ + sn-glycero-3-phosphoethanolamineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine = 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + H+ + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2 2-hexadecanoyl-sn-glycero-3-phosphocholine = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-O-(9Z)-octadecenoyl-2-O-acetyl-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 2-acetyl-sn-glycero-3-phosphocholine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) = a 1-acyl-2-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine = 1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 19 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
- asparaginase activity Source: GO_Central
- lysophospholipase activity Source: UniProtKB
- phosphatidyl phospholipase B activity Source: UniProtKB-EC
- transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB
GO - Biological processi
- cellular amino acid biosynthetic process Source: Reactome
- lipid catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Transferase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS15413-MONOMER |
PathwayCommonsi | Q86U10 |
Reactomei | R-HSA-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: 60 kDa lysophospholipase (EC:3.1.1.5By similarity)Short name: LysoLP Alternative name(s): Lysophospholipase-transacylaseBy similarity Including the following 2 domains: |
Gene namesi | Name:ASPG Synonyms:C14orf76 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000166183.15 |
HGNCi | HGNC:20123, ASPG |
MIMi | 618472, gene |
neXtProti | NX_Q86U10 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 374569 |
OpenTargetsi | ENSG00000166183 |
Miscellaneous databases
Pharosi | Q86U10, Tbio |
Chemistry databases
DrugBanki | DB00721, Procaine |
Polymorphism and mutation databases
BioMutai | ASPG |
DMDMi | 317373428 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000324163 | 1 – 573 | 60 kDa lysophospholipaseAdd BLAST | 573 |
Proteomic databases
MassIVEi | Q86U10 |
PaxDbi | Q86U10 |
PeptideAtlasi | Q86U10 |
PRIDEi | Q86U10 |
ProteomicsDBi | 69756 [Q86U10-1] 69757 [Q86U10-3] |
PTM databases
PhosphoSitePlusi | Q86U10 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166183, Expressed in right lobe of liver and 103 other tissues |
ExpressionAtlasi | Q86U10, baseline and differential |
Genevisiblei | Q86U10, HS |
Organism-specific databases
HPAi | ENSG00000166183, Group enriched (heart muscle, liver) |
Interactioni
Subunit structurei
Monomer.
By similarityBinary interactionsi
Hide detailsQ86U10
Protein-protein interaction databases
BioGRIDi | 131909, 13 interactors |
IntActi | Q86U10, 11 interactors |
STRINGi | 9606.ENSP00000450040 |
Miscellaneous databases
RNActi | Q86U10, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 355 | Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST | 347 | |
Repeati | 141 – 170 | ANK 1Add BLAST | 30 | |
Repeati | 399 – 429 | ANK 2Add BLAST | 31 | |
Repeati | 433 – 462 | ANK 3Add BLAST | 30 | |
Repeati | 466 – 495 | ANK 4Add BLAST | 30 | |
Repeati | 533 – 562 | ANK 5Add BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 350 | AsparaginaseAdd BLAST | 310 | |
Regioni | 84 – 86 | Substrate bindingBy similarity | 3 | |
Regioni | 116 – 117 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
In the N-terminal section; belongs to the asparaginase 1 family.Curated
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG0503, Eukaryota |
GeneTreei | ENSGT00390000001610 |
HOGENOMi | CLU_019134_3_0_1 |
InParanoidi | Q86U10 |
OMAi | QYGADLC |
OrthoDBi | 886725at2759 |
PhylomeDBi | Q86U10 |
TreeFami | TF315247 |
Family and domain databases
CDDi | cd08963, L-asparaginase_I, 1 hit |
Gene3Di | 1.25.40.20, 2 hits 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR006033, AsnA_fam IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR027473, L-asparaginase_C IPR041725, L-asparaginase_I IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit |
PIRSFi | PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR01415, ANKYRIN PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00248, ANK, 4 hits SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00519, asnASE_I, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q86U10-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH
60 70 80 90 100
ARARGLSEDT LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI
110 120 130 140 150
KRHYEQYHGF VVIHGTDTMA FAASMLSFML ENLQKTVILT GAQVPIHALW
160 170 180 190 200
SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRATKVD ARRFAAFCSP
210 220 230 240 250
NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL LRLYPGIPAA
260 270 280 290 300
LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL
310 320 330 340 350
QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE
360 370 380 390 400
LLTKDLRGEM TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL
410 420 430 440 450
VPSLACAAAH AGDVEALQAL VELGSDLGLV DFNGQTPLHA AARGGHTEAV
460 470 480 490 500
TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP GVIGLLREAG ASLSTQELEE
510 520 530 540 550
AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV AEAAGNLAVV
560 570
AFLQSLEGAV GAQAPCPEVL PGV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V1Y8 | G3V1Y8_HUMAN | Asparaginase | ASPG hCG_23215 | 555 | Annotation score: | ||
H0YHG5 | H0YHG5_HUMAN | Asparaginase | ASPG | 126 | Annotation score: | ||
H0YHD3 | H0YHD3_HUMAN | 60 kDa lysophospholipase | ASPG | 140 | Annotation score: |
Sequence cautioni
The sequence CAD62331 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059131 | 95 | C → R. Corresponds to variant dbSNP:rs1770984Ensembl. | 1 | |
Natural variantiVAR_059132 | 96 | L → V. Corresponds to variant dbSNP:rs1744284Ensembl. | 1 | |
Natural variantiVAR_059133 | 344 | S → R1 PublicationCorresponds to variant dbSNP:rs8012505Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040740 | 568 | E → EPSCFQE in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136001 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81858.1 CH471061 Genomic DNA Translation: EAW81859.1 BC035836 mRNA Translation: AAH35836.1 BC136637 mRNA Translation: AAI36638.1 BX247999 mRNA Translation: CAD62331.1 Sequence problems. AI373537 mRNA No translation available. |
CCDSi | CCDS45170.2 [Q86U10-1] |
RefSeqi | NP_001073933.2, NM_001080464.2 [Q86U10-1] XP_005267647.1, XM_005267590.4 [Q86U10-3] |
Genome annotation databases
Ensembli | ENST00000551177; ENSP00000450040; ENSG00000166183 [Q86U10-1] |
GeneIDi | 374569 |
KEGGi | hsa:374569 |
UCSCi | uc001yoq.3, human [Q86U10-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL136001 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81858.1 CH471061 Genomic DNA Translation: EAW81859.1 BC035836 mRNA Translation: AAH35836.1 BC136637 mRNA Translation: AAI36638.1 BX247999 mRNA Translation: CAD62331.1 Sequence problems. AI373537 mRNA No translation available. |
CCDSi | CCDS45170.2 [Q86U10-1] |
RefSeqi | NP_001073933.2, NM_001080464.2 [Q86U10-1] XP_005267647.1, XM_005267590.4 [Q86U10-3] |
3D structure databases
SMRi | Q86U10 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 131909, 13 interactors |
IntActi | Q86U10, 11 interactors |
STRINGi | 9606.ENSP00000450040 |
Chemistry databases
DrugBanki | DB00721, Procaine |
PTM databases
PhosphoSitePlusi | Q86U10 |
Polymorphism and mutation databases
BioMutai | ASPG |
DMDMi | 317373428 |
Proteomic databases
MassIVEi | Q86U10 |
PaxDbi | Q86U10 |
PeptideAtlasi | Q86U10 |
PRIDEi | Q86U10 |
ProteomicsDBi | 69756 [Q86U10-1] 69757 [Q86U10-3] |
Protocols and materials databases
Antibodypediai | 59083, 58 antibodies |
Genome annotation databases
Ensembli | ENST00000551177; ENSP00000450040; ENSG00000166183 [Q86U10-1] |
GeneIDi | 374569 |
KEGGi | hsa:374569 |
UCSCi | uc001yoq.3, human [Q86U10-1] |
Organism-specific databases
CTDi | 374569 |
DisGeNETi | 374569 |
EuPathDBi | HostDB:ENSG00000166183.15 |
GeneCardsi | ASPG |
HGNCi | HGNC:20123, ASPG |
HPAi | ENSG00000166183, Group enriched (heart muscle, liver) |
MIMi | 618472, gene |
neXtProti | NX_Q86U10 |
OpenTargetsi | ENSG00000166183 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0503, Eukaryota |
GeneTreei | ENSGT00390000001610 |
HOGENOMi | CLU_019134_3_0_1 |
InParanoidi | Q86U10 |
OMAi | QYGADLC |
OrthoDBi | 886725at2759 |
PhylomeDBi | Q86U10 |
TreeFami | TF315247 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS15413-MONOMER |
PathwayCommonsi | Q86U10 |
Reactomei | R-HSA-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 374569, 2 hits in 837 CRISPR screens |
GenomeRNAii | 374569 |
Pharosi | Q86U10, Tbio |
PROi | PR:Q86U10 |
RNActi | Q86U10, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166183, Expressed in right lobe of liver and 103 other tissues |
ExpressionAtlasi | Q86U10, baseline and differential |
Genevisiblei | Q86U10, HS |
Family and domain databases
CDDi | cd08963, L-asparaginase_I, 1 hit |
Gene3Di | 1.25.40.20, 2 hits 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR006033, AsnA_fam IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR027473, L-asparaginase_C IPR041725, L-asparaginase_I IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit |
PIRSFi | PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR01415, ANKYRIN PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00248, ANK, 4 hits SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00519, asnASE_I, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPP60_HUMAN | |
Accessioni | Q86U10Primary (citable) accession number: Q86U10 Secondary accession number(s): B9EGQ2, Q8IV80 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | January 11, 2011 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations