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Protein

Acyl-coenzyme A thioesterase 1

Gene

ACOT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

Kineticsi

  1. KM=35.8 µM for C10-acyl-CoA1 Publication
  2. KM=3.6 µM for C12-acyl-CoA1 Publication
  3. KM=2.8 µM for C14-acyl-CoA1 Publication
  4. KM=3.6 µM for C16-acyl-CoA1 Publication
  5. KM=2.4 µM for C18-acyl-CoA1 Publication
  6. KM=2 µM for C20-acyl-CoA1 Publication
  7. KM=2.4 µM for C16:1-acyl-CoA1 Publication
  8. KM=4.1 µM for C18:1-acyl-CoA1 Publication
  9. KM=2.1 µM for C18:1-trans-acyl-CoA1 Publication
  1. Vmax=224 nmol/min/mg enzyme toward C10-acyl-CoA1 Publication
  2. Vmax=700 nmol/min/mg enzyme toward C12-acyl-CoA1 Publication
  3. Vmax=912 nmol/min/mg enzyme toward C14-acyl-CoA1 Publication
  4. Vmax=691 nmol/min/mg enzyme toward C16-acyl-CoA1 Publication
  5. Vmax=597 nmol/min/mg enzyme toward C18-acyl-CoA1 Publication
  6. Vmax=520 nmol/min/mg enzyme toward C20-acyl-CoA1 Publication
  7. Vmax=577 nmol/min/mg enzyme toward C16:1-acyl-CoA1 Publication
  8. Vmax=258 nmol/min/mg enzyme toward C18:1-acyl-CoA1 Publication
  9. Vmax=309 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Charge relay systemBy similarity1
Active sitei326Charge relay systemBy similarity1
Active sitei360Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • acyl-CoA metabolic process Source: HGNC
  • long-chain fatty acid metabolic process Source: HGNC
  • very long-chain fatty acid metabolic process Source: HGNC

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14105
BRENDAi3.1.2.2 2681
ReactomeiR-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
SABIO-RKiQ86TX2

Protein family/group databases

ESTHERihuman-ACOT1 Acyl-CoA_Thioesterase

Chemistry databases

SwissLipidsiSLP:000000613

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 1 (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 1
Alternative name(s):
CTE-I
CTE-Ib
Inducible cytosolic acyl-coenzyme A thioester hydrolase
Long chain acyl-CoA thioester hydrolase
Short name:
Long chain acyl-CoA hydrolase
Gene namesi
Name:ACOT1
Synonyms:CTE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000184227.7
HGNCiHGNC:33128 ACOT1
MIMi614313 gene
neXtProtiNX_Q86TX2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi641371
OpenTargetsiENSG00000184227
PharmGKBiPA162375318

Chemistry databases

ChEMBLiCHEMBL2189136

Polymorphism and mutation databases

BioMutaiACOT1
DMDMi50428913

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002021552 – 421Acyl-coenzyme A thioesterase 1Add BLAST420

Proteomic databases

EPDiQ86TX2
MaxQBiQ86TX2
PaxDbiQ86TX2
PeptideAtlasiQ86TX2
PRIDEiQ86TX2
ProteomicsDBi69743

2D gel databases

UCD-2DPAGEiQ86TX2

PTM databases

iPTMnetiQ86TX2
PhosphoSitePlusiQ86TX2
SwissPalmiQ86TX2

Expressioni

Gene expression databases

BgeeiENSG00000184227
CleanExiHS_ACOT1
ExpressionAtlasiQ86TX2 baseline and differential
GenevisibleiQ86TX2 HS

Organism-specific databases

HPAiHPA043705

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi534965, 10 interactors
IntActiQ86TX2, 13 interactors
STRINGi9606.ENSP00000311224

Structurei

3D structure databases

ProteinModelPortaliQ86TX2
SMRiQ86TX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG410II3X Eukaryota
COG1073 LUCA
GeneTreeiENSGT00390000001046
HOGENOMiHOG000116219
HOVERGENiHBG000331
InParanoidiQ86TX2
KOiK01068
OMAiPETEIPW
OrthoDBiEOG091G08KU
PhylomeDBiQ86TX2
TreeFamiTF314911

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR016662 Acyl-CoA_thioEstase_long-chain
IPR014940 BAAT_C
IPR006862 Thio_Ohase/aa_AcTrfase
PfamiView protein in Pfam
PF08840 BAAT_C, 1 hit
PF04775 Bile_Hydr_Trans, 1 hit
PIRSFiPIRSF016521 Acyl-CoA_hydro, 1 hit
SUPFAMiSSF53474 SSF53474, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86TX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATLILEPA GRCCWDEPVR IAVRGLAPEQ PVTLRASLRD EKGALFQAHA
60 70 80 90 100
RYRADTLGEL DLERAPALGG SFAGLEPMGL LWALEPEKPL VRLVKRDVRT
110 120 130 140 150
PLAVELEVLD GHDPDPGRLL CRVRHERYFL PPGVRREPVR AGRVRGTLFL
160 170 180 190 200
PPEPGPFPGI VDMFGTGGGL LEYRASLLAG KGFAVMALAY YNYEDLPKTM
210 220 230 240 250
ETLHLEYFEE AVNYLLSHPE VKGPGVGLLG ISKGGELCLS MASFLKGITA
260 270 280 290 300
AVVINGSVAN VGGTLRYKGE TLPPVGVNRN RIKVTKDGYA DIVDVLNSPL
310 320 330 340 350
EGPDQKSFIP VERAESTFLF LVGQDDHNWK SEFYANEACK RLQAHGRRKP
360 370 380 390 400
QIICYPETGH YIEPPYFPLC RASLHALVGS PIIWGGEPRA HAMAQVDAWK
410 420
QLQTFFHKHL GGHEGTIPSK V
Length:421
Mass (Da):46,277
Last modified:June 1, 2003 - v1
Checksum:i04675F28D9D53B97
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059830266R → H. Corresponds to variant dbSNP:rs1049568Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ082754 mRNA Translation: AAZ31236.1
BX161396 mRNA Translation: CAD61883.1
BC127748 mRNA Translation: AAI27749.1
BC132889 mRNA Translation: AAI32890.1
BC132891 mRNA Translation: AAI32892.1
BC143042 mRNA Translation: AAI43043.1
CCDSiCCDS32117.1
RefSeqiNP_001032238.1, NM_001037161.1
UniGeneiHs.446685
Hs.568046

Genome annotation databases

EnsembliENST00000311148; ENSP00000311224; ENSG00000184227
GeneIDi641371
KEGGihsa:641371
UCSCiuc001xol.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiACOT1_HUMAN
AccessioniPrimary (citable) accession number: Q86TX2
Secondary accession number(s): A1L173, Q3I5F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: June 20, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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