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Entry version 144 (17 Jun 2020)
Sequence version 1 (01 Jun 2003)
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Protein

Actin-histidine N-methyltransferase

Gene

SETD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73' (PubMed:30526847, PubMed:30626964, PubMed:30785395). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed:30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed:30626964, PubMed:30785395).3 Publications

Caution

Was initially reported to have histone methyltransferase activity and methylate 'Lys-4' and 'Lys-36' of histone H3 (H3K4me and H3K36me) (By similarity). However, this conclusion was based on mass spectrometry data wherin mass shifts were inconsistent with a bona fide methylation event (PubMed:30626964). In vitro, the protein-lysine methyltransferase activity is weak compared to the protein-histidine methyltransferase activity (PubMed:30526847).By similarity2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat is 0.65 min(-1) with beta-actin as substrate (PubMed:30526847). Kcat is 0.809 min(-1) with beta-actin as substrate (PubMed:30785395). Kcat is 0.61 min(-1) with S-adenosyl-L-methionine as substrate (PubMed:30526847). Kcat is 0.673 min(-1) with S-adenosyl-L-methionine as substrate (PubMed:30785395).2 Publications
  1. KM=0.752 µM for beta-actin1 Publication
  2. KM=0.502 µM for beta-actin1 Publication
  3. KM=0.116 µM for S-adenosyl-L-methionine1 Publication
  4. KM=0.111 µM for S-adenosyl-L-methionine1 Publication
  1. Vmax=9.091 nmol/min/mg enzyme with beta-actin as substrate1 Publication
  2. Vmax=13.550 nmol/min/mg enzyme with beta-actin as substrate1 Publication
  3. Vmax=8.649 nmol/min/mg enzyme with S-adenosyl-L-methionine as substrate1 Publication
  4. Vmax=11.260 nmol/min/mg enzyme with S-adenosyl-L-methionine as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei75S-adenosyl-L-methionineCombined sources3 Publications1
Binding sitei254S-adenosyl-L-methionineCombined sources3 Publications1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActin-binding, Methyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-3214841 PKMTs methylate histone lysines

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Actin-histidine N-methyltransferaseCurated (EC:2.1.1.852 Publications)
Alternative name(s):
SET domain-containing protein 3Curated
Short name:
hSETD31 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SETD32 PublicationsImported
Synonyms:C14orf154Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 14

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
HostDB:ENSG00000183576.12

Human Gene Nomenclature Database

More...
HGNCi
HGNC:20493 SETD3

Online Mendelian Inheritance in Man (OMIM)

More...
MIMi
615671 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q86TU7

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi37 – 42SSPAPG → ASPAPA: Does not affect ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication6
Mutagenesisi181 – 185SEYDT → AEYDA: Decreased ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication5
Mutagenesisi215R → A: Decreased binding to actin and decreased protein-histidine N-methyltransferase activity. 1 Publication1
Mutagenesisi256N → A: Decreased binding to actin and decreased protein-histidine N-methyltransferase activity. 1 Publication1
Mutagenesisi260 – 264TEDGS → AEDGA: Does not affect ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication5
Mutagenesisi313Y → A: Abolished protein-histidine N-methyltransferase activity. 1 Publication1
Mutagenesisi313Y → F: Strongly decreased binding to actin and decreased protein-histidine N-methyltransferase activity. 1 Publication1
Mutagenesisi316R → A: Decreased binding to actin and decreased protein-histidine N-methyltransferase activity. 1 Publication1
Mutagenesisi373 – 378TEPPIS → AEPPIA: Strongly decreased ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication6
Mutagenesisi512 – 517SSVGDS → ASVGDA: Does not affect ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication6
Mutagenesisi565 – 569SENES → AENEA: Does not affect ubiquitination and degradation by the SCF(FBXW7) complex. 1 Publication5

Organism-specific databases

DisGeNET

More...
DisGeNETi
84193

Open Targets

More...
OpenTargetsi
ENSG00000183576

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA134883013

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q86TU7 Tdark

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
SETD3

Domain mapping of disease mutations (DMDM)

More...
DMDMi
74750394

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002541751 – 594Actin-histidine N-methyltransferaseAdd BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei513PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by GSK3B, which is required for recognition by the SCF(FBXW7) complex and subsequent degradation.1 Publication
Ubiquitinated by the SCF(FBXW7) complex following phosphorylation by GSK3B, leading to its degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q86TU7

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q86TU7

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q86TU7

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q86TU7

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q86TU7

PeptideAtlas

More...
PeptideAtlasi
Q86TU7

PRoteomics IDEntifications database

More...
PRIDEi
Q86TU7

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
69732 [Q86TU7-1]
69733 [Q86TU7-2]
69734 [Q86TU7-3]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q86TU7

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q86TU7

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Protein levels peak in S phase and are lowest during M phase (at protein level).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000183576 Expressed in testis and 238 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q86TU7 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q86TU7 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000183576 Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with MYOD1.

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
123940, 18 interactors

Protein interaction database and analysis system

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IntActi
Q86TU7, 8 interactors

Molecular INTeraction database

More...
MINTi
Q86TU7

STRING: functional protein association networks

More...
STRINGi
9606.ENSP00000327436

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q86TU7 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q86TU7

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini94 – 314SETPROSITE-ProRule annotationAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni104 – 106S-adenosyl-L-methionine bindingCombined sources3 Publications3
Regioni275 – 279S-adenosyl-L-methionine bindingCombined sources3 Publications5
Regioni325 – 327S-adenosyl-L-methionine bindingCombined sources3 Publications3

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The SET domain specifically recognizes and binds actin, suggesting that it does not accommodate substrates diverging from actin.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1337 Eukaryota
ENOG410Y7DR LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000153577

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_028272_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q86TU7

KEGG Orthology (KO)

More...
KOi
K19199

Identification of Orthologs from Complete Genome Data

More...
OMAi
KWALCTV

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q86TU7

TreeFam database of animal gene trees

More...
TreeFami
TF354226

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.90.1420.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR015353 Rubisco_LSMT_subst-bd
IPR036464 Rubisco_LSMT_subst-bd_sf
IPR001214 SET_dom
IPR025785 SETD3

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF09273 Rubis-subs-bind, 1 hit
PF00856 SET, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF81822 SSF81822, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51565 SAM_MT85_SETD3, 1 hit
PS50280 SET, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q86TU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKKSRVKTQ KSGTGATATV SPKEILNLTS ELLQKCSSPA PGPGKEWEEY
60 70 80 90 100
VQIRTLVEKI RKKQKGLSVT FDGKREDYFP DLMKWASENG ASVEGFEMVN
110 120 130 140 150
FKEEGFGLRA TRDIKAEELF LWVPRKLLMT VESAKNSVLG PLYSQDRILQ
160 170 180 190 200
AMGNIALAFH LLCERASPNS FWQPYIQTLP SEYDTPLYFE EDEVRYLQST
210 220 230 240 250
QAIHDVFSQY KNTARQYAYF YKVIQTHPHA NKLPLKDSFT YEDYRWAVSS
260 270 280 290 300
VMTRQNQIPT EDGSRVTLAL IPLWDMCNHT NGLITTGYNL EDDRCECVAL
310 320 330 340 350
QDFRAGEQIY IFYGTRSNAE FVIHSGFFFD NNSHDRVKIK LGVSKSDRLY
360 370 380 390 400
AMKAEVLARA GIPTSSVFAL HFTEPPISAQ LLAFLRVFCM TEEELKEHLL
410 420 430 440 450
GDSAIDRIFT LGNSEFPVSW DNEVKLWTFL EDRASLLLKT YKTTIEEDKS
460 470 480 490 500
VLKNHDLSVR AKMAIKLRLG EKEILEKAVK SAAVNREYYR QQMEEKAPLP
510 520 530 540 550
KYEESNLGLL ESSVGDSRLP LVLRNLEEEA GVQDALNIRE AISKAKATEN
560 570 580 590
GLVNGENSIP NGTRSENESL NQESKRAVED AKGSSSDSTA GVKE
Length:594
Mass (Da):67,257
Last modified:June 1, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDF27C8B133A19E16
GO
Isoform 2 (identifier: Q86TU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-296: ITTGYNLEDDRCE → TPEDSFALAVASA
     297-594: Missing.

Show »
Length:296
Mass (Da):33,701
Checksum:iB853D7B95F23F98B
GO
Isoform 3 (identifier: Q86TU7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: I → V
     285-594: Missing.

Show »
Length:284
Mass (Da):32,540
Checksum:iCAF5DC1F9A724072
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q6NXR6Q6NXR6_HUMAN
Protein-histidine N-methyltransfera...
SETD3
292Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9K0W5C9K0W5_HUMAN
Actin-histidine N-methyltransferase
SETD3
81Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAB15525 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti415E → K in AAI42996 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_028830278N → D. Corresponds to variant dbSNP:rs1740231Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_021190284 – 296ITTGY…DDRCE → TPEDSFALAVASA in isoform 2. 2 PublicationsAdd BLAST13
Alternative sequenceiVSP_021191284I → V in isoform 3. 1 Publication1
Alternative sequenceiVSP_021192285 – 594Missing in isoform 3. 1 PublicationAdd BLAST310
Alternative sequenceiVSP_021193297 – 594Missing in isoform 2. 2 PublicationsAdd BLAST298

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
BX161441 mRNA Translation: CAD61911.1
BX161471 mRNA Translation: CAD61927.1
AK026680 mRNA Translation: BAB15525.1 Frameshift.
AK302882 mRNA Translation: BAG64060.1
AL110504 Genomic DNA No translation available.
AL132819 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81664.1
CH471061 Genomic DNA Translation: EAW81665.1
BC009054 mRNA Translation: AAH09054.2
BC120967 mRNA Translation: AAI20968.1
BC120968 mRNA Translation: AAI20969.1
BC127624 mRNA Translation: AAI27625.1
BC127625 mRNA Translation: AAI27626.1
BC142995 mRNA Translation: AAI42996.1
BC148251 mRNA Translation: AAI48252.1
AL359581 mRNA Translation: CAH56365.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS9951.1 [Q86TU7-1]
CCDS9952.1 [Q86TU7-2]

Protein sequence database of the Protein Information Resource

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PIRi
T50614

NCBI Reference Sequences

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RefSeqi
NP_115609.2, NM_032233.2 [Q86TU7-1]
NP_954574.1, NM_199123.1 [Q86TU7-2]
XP_011535533.2, XM_011537231.2 [Q86TU7-1]
XP_011535534.1, XM_011537232.2 [Q86TU7-1]
XP_011535537.1, XM_011537235.1 [Q86TU7-2]
XP_016877188.1, XM_017021699.1 [Q86TU7-1]
XP_016877189.1, XM_017021700.1 [Q86TU7-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000329331; ENSP00000327910; ENSG00000183576 [Q86TU7-2]
ENST00000331768; ENSP00000327436; ENSG00000183576 [Q86TU7-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
84193

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:84193

UCSC genome browser

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UCSCi
uc001ygc.4 human [Q86TU7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Protein Spotlight

On versatility - Issue 215 of June 2019

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX161441 mRNA Translation: CAD61911.1
BX161471 mRNA Translation: CAD61927.1
AK026680 mRNA Translation: BAB15525.1 Frameshift.
AK302882 mRNA Translation: BAG64060.1
AL110504 Genomic DNA No translation available.
AL132819 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81664.1
CH471061 Genomic DNA Translation: EAW81665.1
BC009054 mRNA Translation: AAH09054.2
BC120967 mRNA Translation: AAI20968.1
BC120968 mRNA Translation: AAI20969.1
BC127624 mRNA Translation: AAI27625.1
BC127625 mRNA Translation: AAI27626.1
BC142995 mRNA Translation: AAI42996.1
BC148251 mRNA Translation: AAI48252.1
AL359581 mRNA Translation: CAH56365.1
CCDSiCCDS9951.1 [Q86TU7-1]
CCDS9952.1 [Q86TU7-2]
PIRiT50614
RefSeqiNP_115609.2, NM_032233.2 [Q86TU7-1]
NP_954574.1, NM_199123.1 [Q86TU7-2]
XP_011535533.2, XM_011537231.2 [Q86TU7-1]
XP_011535534.1, XM_011537232.2 [Q86TU7-1]
XP_011535537.1, XM_011537235.1 [Q86TU7-2]
XP_016877188.1, XM_017021699.1 [Q86TU7-1]
XP_016877189.1, XM_017021700.1 [Q86TU7-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SMTX-ray2.04A2-498[»]
6ICTX-ray1.95A/B/C/D1-503[»]
6ICVX-ray2.15A/B1-503[»]
6JATX-ray2.71A/C1-498[»]
6MBJX-ray1.78A/B21-500[»]
A/B1-594[»]
6MBKX-ray1.69A/B21-503[»]
A/B1-594[»]
6MBLX-ray2.20A21-500[»]
A1-594[»]
6OX0X-ray1.75A/B1-594[»]
6OX1X-ray1.95A/B1-594[»]
6OX2X-ray2.09A/B1-594[»]
6OX3X-ray1.78A/B1-594[»]
6OX4X-ray2.29A/B1-594[»]
6OX5X-ray2.10A1-594[»]
6V62X-ray2.36A1-594[»]
6V63X-ray2.02A/B1-594[»]
SMRiQ86TU7
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi123940, 18 interactors
IntActiQ86TU7, 8 interactors
MINTiQ86TU7
STRINGi9606.ENSP00000327436

PTM databases

iPTMnetiQ86TU7
PhosphoSitePlusiQ86TU7

Polymorphism and mutation databases

BioMutaiSETD3
DMDMi74750394

Proteomic databases

EPDiQ86TU7
jPOSTiQ86TU7
MassIVEiQ86TU7
MaxQBiQ86TU7
PaxDbiQ86TU7
PeptideAtlasiQ86TU7
PRIDEiQ86TU7
ProteomicsDBi69732 [Q86TU7-1]
69733 [Q86TU7-2]
69734 [Q86TU7-3]

Protocols and materials databases

ABCD curated depository of sequenced antibodies

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ABCDi
Q86TU7 1 sequenced antibody

Antibodypedia a portal for validated antibodies

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Antibodypediai
147 151 antibodies

Genome annotation databases

EnsembliENST00000329331; ENSP00000327910; ENSG00000183576 [Q86TU7-2]
ENST00000331768; ENSP00000327436; ENSG00000183576 [Q86TU7-1]
GeneIDi84193
KEGGihsa:84193
UCSCiuc001ygc.4 human [Q86TU7-1]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
84193
DisGeNETi84193
EuPathDBiHostDB:ENSG00000183576.12

GeneCards: human genes, protein and diseases

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GeneCardsi
SETD3
HGNCiHGNC:20493 SETD3
HPAiENSG00000183576 Low tissue specificity
MIMi615671 gene
neXtProtiNX_Q86TU7
OpenTargetsiENSG00000183576
PharmGKBiPA134883013

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1337 Eukaryota
ENOG410Y7DR LUCA
GeneTreeiENSGT00940000153577
HOGENOMiCLU_028272_0_0_1
InParanoidiQ86TU7
KOiK19199
OMAiKWALCTV
PhylomeDBiQ86TU7
TreeFamiTF354226

Enzyme and pathway databases

ReactomeiR-HSA-3214841 PKMTs methylate histone lysines

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
84193 2 hits in 791 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
SETD3 human

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
84193
PharosiQ86TU7 Tdark

Protein Ontology

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PROi
PR:Q86TU7
RNActiQ86TU7 protein

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSG00000183576 Expressed in testis and 238 other tissues
ExpressionAtlasiQ86TU7 baseline and differential
GenevisibleiQ86TU7 HS

Family and domain databases

Gene3Di3.90.1420.10, 1 hit
InterProiView protein in InterPro
IPR015353 Rubisco_LSMT_subst-bd
IPR036464 Rubisco_LSMT_subst-bd_sf
IPR001214 SET_dom
IPR025785 SETD3
PfamiView protein in Pfam
PF09273 Rubis-subs-bind, 1 hit
PF00856 SET, 1 hit
SUPFAMiSSF81822 SSF81822, 1 hit
PROSITEiView protein in PROSITE
PS51565 SAM_MT85_SETD3, 1 hit
PS50280 SET, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSETD3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q86TU7
Secondary accession number(s): A0PJU3
, A5PLP0, B4DZE8, Q0VAQ2, Q659C0, Q86TU8, Q96GY9, Q9H5U5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2003
Last modified: June 17, 2020
This is version 144 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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