UniProtKB - Q86T24 (KAISO_HUMAN)
Protein
Transcriptional regulator Kaiso
Gene
ZBTB33
Organism
Homo sapiens (Human)
Status
Functioni
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).6 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 494 – 516 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 522 – 544 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 550 – 573 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- methyl-CpG binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- intracellular signal transduction Source: ProtInc
- negative regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q86T24 |
SignaLinki | Q86T24 |
SIGNORi | Q86T24 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulator KaisoAlternative name(s): Zinc finger and BTB domain-containing protein 33 |
Gene namesi | Name:ZBTB33 Synonyms:KAISO, ZNF348 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16682, ZBTB33 |
MIMi | 300329, gene |
neXtProti | NX_Q86T24 |
VEuPathDBi | HostDB:ENSG00000177485.6 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: ProtInc
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- chromatin Source: NTNU_SB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 552 | C → R: Abrogates both sequence-specific and methylation-dependent DNA-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10009 |
OpenTargetsi | ENSG00000177485 |
PharmGKBi | PA134928604 |
Miscellaneous databases
Pharosi | Q86T24, Tbio |
Genetic variation databases
BioMutai | ZBTB33 |
DMDMi | 84029319 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046988 | 1 – 672 | Transcriptional regulator KaisoAdd BLAST | 672 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 151 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 153 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 251 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 390 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 407 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 414 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 449 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 465 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 474 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 479 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 539 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 570 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 582 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 611 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 618 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q86T24 |
jPOSTi | Q86T24 |
MassIVEi | Q86T24 |
MaxQBi | Q86T24 |
PaxDbi | Q86T24 |
PeptideAtlasi | Q86T24 |
PRIDEi | Q86T24 |
ProteomicsDBi | 69660 |
PTM databases
iPTMneti | Q86T24 |
PhosphoSitePlusi | Q86T24 |
Expressioni
Tissue specificityi
Expressed in vascular endothelium.1 Publication
Inductioni
Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.1 Publication
Gene expression databases
Bgeei | ENSG00000177485, Expressed in secondary oocyte and 218 other tissues |
Genevisiblei | Q86T24, HS |
Organism-specific databases
HPAi | ENSG00000177485, Low tissue specificity |
Interactioni
Subunit structurei
Self-associates.
Interacts with CTNND2 (By similarity).
Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA.
Interacts with NCOR1.
Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein.
Interacts with CBFA2T3.
By similarity5 PublicationsBinary interactionsi
Hide detailsQ86T24
Protein-protein interaction databases
BioGRIDi | 115327, 73 interactors |
CORUMi | Q86T24 |
IntActi | Q86T24, 39 interactors |
MINTi | Q86T24 |
STRINGi | 9606.ENSP00000314153 |
Miscellaneous databases
RNActi | Q86T24, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q86T24 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q86T24 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 94 | BTBPROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 136 | Self-associationBy similarityAdd BLAST | 136 | |
Regioni | 1 – 103 | Interaction with NCOR11 PublicationAdd BLAST | 103 | |
Regioni | 298 – 573 | Interaction with CBFA2T31 PublicationAdd BLAST | 276 | |
Regioni | 454 – 672 | Interaction with CTNND1By similarityAdd BLAST | 219 | |
Regioni | 514 – 638 | Required for DNA-bindingBy similarityAdd BLAST | 125 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 471 – 480 | Nuclear localization signalBy similarity | 10 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 494 – 516 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 522 – 544 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 550 – 573 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157481 |
HOGENOMi | CLU_024688_0_0_1 |
InParanoidi | Q86T24 |
OMAi | GKTFYVC |
OrthoDBi | 567120at2759 |
PhylomeDBi | Q86T24 |
TreeFami | TF333100 |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
i Sequence
Sequence statusi: Complete.
Q86T24-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL
60 70 80 90 100
SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE
110 120 130 140 150
LIKSGQLLGV KFIAELGVPL SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ
160 170 180 190 200
KSKDEAQDNG ATIMPIITES FSLSAEDYEM KKIIVTDSDD DDDDVIFCSE
210 220 230 240 250
ILPTKETLPS NNTVAQVQSN PGPVAISDVA PSASNNSPPL TNITPTQKLP
260 270 280 290 300
TPVNQATLSQ TQGSEKLLVS SAPTHLTPNI ILLNQTPLST PPNVSSSLPN
310 320 330 340 350
HMPSSINLLV QNQQTPNSAI LTGNKANEEE EEEIIDDDDD TISSSPDSAV
360 370 380 390 400
SNTSLVPQAD TSQNTSFDGS LIQKMQIPTL LQEPLSNSLK ISDIITRNTN
410 420 430 440 450
DPGVGSKHLM EGQKIITLDT ATEIEGLSTG CKVYANIGED TYDIVIPVKD
460 470 480 490 500
DPDEGEARLE NEIPKTSGSE MANKRMKVKH DDHYELIVDG RVYYICIVCK
510 520 530 540 550
RSYVCLTSLR RHFNIHSWEK KYPCRYCEKV FPLAEYRTKH EIHHTGERRY
560 570 580 590 600
QCLACGKSFI NYQFMSSHIK SVHSQDPSGD SKLYRLHPCR SLQIRQYAYL
610 620 630 640 650
SDRSSTIPAM KDDGIGYKVD TGKEPPVGTT TSTQNKPMTW EDIFIQQEND
660 670
SIFKQNVTDG STEFEFIIPE SY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | D → Y in CAD91170 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 179 | E → G in CAD91170 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 189 | D → DD in CAD91170 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 189 | D → DD in CAD97963 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 189 | D → DD in AAH42753 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 362 | S → Y in CAD91170 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 670 | E → V in CAD98016 (PubMed:17974005).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK312321 mRNA Translation: BAG35243.1 AL833856 mRNA Translation: CAD38715.1 AL833604 mRNA Translation: CAD91170.1 BX538016 mRNA Translation: CAD97963.1 BX538101 mRNA Translation: CAD98016.1 AC002086 Genomic DNA Translation: AAB54087.1 CH471107 Genomic DNA Translation: EAX11891.1 BC042753 mRNA Translation: AAH42753.1 |
CCDSi | CCDS14596.1 |
RefSeqi | NP_001171671.1, NM_001184742.1 NP_006768.1, NM_006777.3 |
Genome annotation databases
Ensembli | ENST00000326624; ENSP00000314153; ENSG00000177485 ENST00000557385; ENSP00000450969; ENSG00000177485 |
GeneIDi | 10009 |
KEGGi | hsa:10009 |
UCSCi | uc004esn.2, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK312321 mRNA Translation: BAG35243.1 AL833856 mRNA Translation: CAD38715.1 AL833604 mRNA Translation: CAD91170.1 BX538016 mRNA Translation: CAD97963.1 BX538101 mRNA Translation: CAD98016.1 AC002086 Genomic DNA Translation: AAB54087.1 CH471107 Genomic DNA Translation: EAX11891.1 BC042753 mRNA Translation: AAH42753.1 |
CCDSi | CCDS14596.1 |
RefSeqi | NP_001171671.1, NM_001184742.1 NP_006768.1, NM_006777.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LT7 | NMR | - | A | 472-604 | [»] | |
3FKC | X-ray | 1.70 | A | 1-114 | [»] | |
3M4T | X-ray | 2.05 | A | 1-122 | [»] | |
3M8V | X-ray | 2.70 | A | 1-122 | [»] | |
4F6M | X-ray | 2.40 | A | 472-604 | [»] | |
4F6N | X-ray | 2.80 | A | 472-604 | [»] | |
5VMU | X-ray | 2.35 | A | 471-604 | [»] | |
5VMV | X-ray | 2.31 | A | 471-604 | [»] | |
5VMW | X-ray | 2.40 | A | 471-604 | [»] | |
5VMX | X-ray | 2.05 | A | 471-604 | [»] | |
5VMY | X-ray | 2.00 | A | 471-604 | [»] | |
5VMZ | X-ray | 2.32 | A | 471-604 | [»] | |
6DF5 | X-ray | 1.82 | A | 471-604 | [»] | |
6DF8 | X-ray | 2.54 | A | 471-604 | [»] | |
6DF9 | X-ray | 2.32 | A | 471-604 | [»] | |
6DFA | X-ray | 1.91 | A | 471-604 | [»] | |
6DFB | X-ray | 1.66 | A | 471-604 | [»] | |
6DFC | X-ray | 1.85 | A | 471-604 | [»] | |
6V8U | X-ray | 2.10 | A | 471-604 | [»] | |
SMRi | Q86T24 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115327, 73 interactors |
CORUMi | Q86T24 |
IntActi | Q86T24, 39 interactors |
MINTi | Q86T24 |
STRINGi | 9606.ENSP00000314153 |
PTM databases
iPTMneti | Q86T24 |
PhosphoSitePlusi | Q86T24 |
Genetic variation databases
BioMutai | ZBTB33 |
DMDMi | 84029319 |
Proteomic databases
EPDi | Q86T24 |
jPOSTi | Q86T24 |
MassIVEi | Q86T24 |
MaxQBi | Q86T24 |
PaxDbi | Q86T24 |
PeptideAtlasi | Q86T24 |
PRIDEi | Q86T24 |
ProteomicsDBi | 69660 |
Protocols and materials databases
Antibodypediai | 401, 205 antibodies |
DNASUi | 10009 |
Genome annotation databases
Ensembli | ENST00000326624; ENSP00000314153; ENSG00000177485 ENST00000557385; ENSP00000450969; ENSG00000177485 |
GeneIDi | 10009 |
KEGGi | hsa:10009 |
UCSCi | uc004esn.2, human |
Organism-specific databases
CTDi | 10009 |
DisGeNETi | 10009 |
GeneCardsi | ZBTB33 |
HGNCi | HGNC:16682, ZBTB33 |
HPAi | ENSG00000177485, Low tissue specificity |
MIMi | 300329, gene |
neXtProti | NX_Q86T24 |
OpenTargetsi | ENSG00000177485 |
PharmGKBi | PA134928604 |
VEuPathDBi | HostDB:ENSG00000177485.6 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157481 |
HOGENOMi | CLU_024688_0_0_1 |
InParanoidi | Q86T24 |
OMAi | GKTFYVC |
OrthoDBi | 567120at2759 |
PhylomeDBi | Q86T24 |
TreeFami | TF333100 |
Enzyme and pathway databases
PathwayCommonsi | Q86T24 |
SignaLinki | Q86T24 |
SIGNORi | Q86T24 |
Miscellaneous databases
BioGRID-ORCSi | 10009, 3 hits in 642 CRISPR screens |
ChiTaRSi | ZBTB33, human |
EvolutionaryTracei | Q86T24 |
GeneWikii | ZBTB33 |
GenomeRNAii | 10009 |
Pharosi | Q86T24, Tbio |
PROi | PR:Q86T24 |
RNActi | Q86T24, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177485, Expressed in secondary oocyte and 218 other tissues |
Genevisiblei | Q86T24, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAISO_HUMAN | |
Accessioni | Q86T24Primary (citable) accession number: Q86T24 Secondary accession number(s): B2R5U6 Q8N3P0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references