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Protein

Transcriptional regulator Kaiso

Gene

ZBTB33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri494 – 516C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri550 – 573C2H2-type 3PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • methyl-CpG binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation, Wnt signaling pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ86T24
SIGNORiQ86T24

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator Kaiso
Alternative name(s):
Zinc finger and BTB domain-containing protein 33
Gene namesi
Name:ZBTB33
Synonyms:KAISO, ZNF348
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000177485.6
HGNCiHGNC:16682 ZBTB33
MIMi300329 gene
neXtProtiNX_Q86T24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi552C → R: Abrogates both sequence-specific and methylation-dependent DNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi10009
OpenTargetsiENSG00000177485
PharmGKBiPA134928604

Polymorphism and mutation databases

BioMutaiZBTB33
DMDMi84029319

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000469881 – 672Transcriptional regulator KaisoAdd BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki153Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei251PhosphothreonineCombined sources1
Cross-linki390Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki407Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki449Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki465Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki474Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki570Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki582Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki611Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki618Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86T24
MaxQBiQ86T24
PaxDbiQ86T24
PeptideAtlasiQ86T24
PRIDEiQ86T24
ProteomicsDBi69660

PTM databases

iPTMnetiQ86T24
PhosphoSitePlusiQ86T24

Expressioni

Tissue specificityi

Expressed in vascular endothelium.1 Publication

Inductioni

Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.1 Publication

Gene expression databases

BgeeiENSG00000177485
CleanExiHS_ZBTB33
GenevisibleiQ86T24 HS

Organism-specific databases

HPAiCAB001980
HPA000755
HPA005732

Interactioni

Subunit structurei

Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. Interacts with CBFA2T3.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIML2Q8N7C33EBI-2515625,EBI-11059915

Protein-protein interaction databases

BioGridi115327, 53 interactors
CORUMiQ86T24
IntActiQ86T24, 29 interactors
MINTiQ86T24
STRINGi9606.ENSP00000314153

Structurei

Secondary structure

1672
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 27Combined sources14
Turni28 – 31Combined sources4
Beta strandi34 – 38Combined sources5
Beta strandi41 – 45Combined sources5
Helixi47 – 53Combined sources7
Helixi55 – 60Combined sources6
Turni61 – 63Combined sources3
Beta strandi66 – 70Combined sources5
Helixi75 – 85Combined sources11
Helixi95 – 97Combined sources3
Helixi98 – 108Combined sources11
Helixi111 – 114Combined sources4
Beta strandi483 – 488Combined sources6
Beta strandi491 – 496Combined sources6
Turni497 – 499Combined sources3
Beta strandi502 – 505Combined sources4
Helixi506 – 517Combined sources12
Beta strandi525 – 528Combined sources4
Beta strandi530 – 533Combined sources4
Helixi534 – 545Combined sources12
Beta strandi550 – 552Combined sources3
Turni553 – 555Combined sources3
Beta strandi558 – 561Combined sources4
Helixi562 – 572Combined sources11
Turni578 – 580Combined sources3
Beta strandi583 – 586Combined sources4
Helixi598 – 600Combined sources3

3D structure databases

ProteinModelPortaliQ86T24
SMRiQ86T24
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86T24

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 94BTBPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 136Self-associationBy similarityAdd BLAST136
Regioni1 – 103Interaction with NCOR11 PublicationAdd BLAST103
Regioni298 – 573Interaction with CBFA2T31 PublicationAdd BLAST276
Regioni454 – 672Interaction with CTNND1By similarityAdd BLAST219
Regioni514 – 638Required for DNA-bindingBy similarityAdd BLAST125

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi471 – 480Nuclear localization signalBy similarity10

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri494 – 516C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri550 – 573C2H2-type 3PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00810000125416
HOGENOMiHOG000230932
HOVERGENiHBG079575
InParanoidiQ86T24
KOiK10507
OMAiHSQDPSG
OrthoDBiEOG091G02SP
PhylomeDBiQ86T24
TreeFamiTF333100

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF54695 SSF54695, 1 hit
SSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequencei

Sequence statusi: Complete.

Q86T24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL
60 70 80 90 100
SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE
110 120 130 140 150
LIKSGQLLGV KFIAELGVPL SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ
160 170 180 190 200
KSKDEAQDNG ATIMPIITES FSLSAEDYEM KKIIVTDSDD DDDDVIFCSE
210 220 230 240 250
ILPTKETLPS NNTVAQVQSN PGPVAISDVA PSASNNSPPL TNITPTQKLP
260 270 280 290 300
TPVNQATLSQ TQGSEKLLVS SAPTHLTPNI ILLNQTPLST PPNVSSSLPN
310 320 330 340 350
HMPSSINLLV QNQQTPNSAI LTGNKANEEE EEEIIDDDDD TISSSPDSAV
360 370 380 390 400
SNTSLVPQAD TSQNTSFDGS LIQKMQIPTL LQEPLSNSLK ISDIITRNTN
410 420 430 440 450
DPGVGSKHLM EGQKIITLDT ATEIEGLSTG CKVYANIGED TYDIVIPVKD
460 470 480 490 500
DPDEGEARLE NEIPKTSGSE MANKRMKVKH DDHYELIVDG RVYYICIVCK
510 520 530 540 550
RSYVCLTSLR RHFNIHSWEK KYPCRYCEKV FPLAEYRTKH EIHHTGERRY
560 570 580 590 600
QCLACGKSFI NYQFMSSHIK SVHSQDPSGD SKLYRLHPCR SLQIRQYAYL
610 620 630 640 650
SDRSSTIPAM KDDGIGYKVD TGKEPPVGTT TSTQNKPMTW EDIFIQQEND
660 670
SIFKQNVTDG STEFEFIIPE SY
Length:672
Mass (Da):74,484
Last modified:December 20, 2005 - v2
Checksum:i76D94B4051056DB5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40D → Y in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti179E → G in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in CAD97963 (PubMed:17974005).Curated1
Sequence conflicti189D → DD in AAH42753 (PubMed:15489334).Curated1
Sequence conflicti362S → Y in CAD91170 (PubMed:17974005).Curated1
Sequence conflicti670E → V in CAD98016 (PubMed:17974005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312321 mRNA Translation: BAG35243.1
AL833856 mRNA Translation: CAD38715.1
AL833604 mRNA Translation: CAD91170.1
BX538016 mRNA Translation: CAD97963.1
BX538101 mRNA Translation: CAD98016.1
AC002086 Genomic DNA Translation: AAB54087.1
CH471107 Genomic DNA Translation: EAX11891.1
BC042753 mRNA Translation: AAH42753.1
CCDSiCCDS14596.1
RefSeqiNP_001171671.1, NM_001184742.1
NP_006768.1, NM_006777.3
UniGeneiHs.143604

Genome annotation databases

EnsembliENST00000326624; ENSP00000314153; ENSG00000177485
ENST00000557385; ENSP00000450969; ENSG00000177485
GeneIDi10009
KEGGihsa:10009
UCSCiuc004esn.2 human

Similar proteinsi

Entry informationi

Entry nameiKAISO_HUMAN
AccessioniPrimary (citable) accession number: Q86T24
Secondary accession number(s): B2R5U6
, O00319, Q7Z361, Q8IVP6, Q8N3P0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 20, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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