UniProtKB - Q86SR1 (GLT10_HUMAN)
Protein
Polypeptide N-acetylgalactosaminyltransferase 10
Gene
GALNT10
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates.
Caution
According to experiments made in rat, this enzyme is unable to transfer GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties, thereby acting as a glycopeptide transferase.Curated
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 154 | Substrate | 1 | |
Binding sitei | 156 | Substrate | 1 | |
Binding sitei | 185 | Substrate | 1 | |
Binding sitei | 214 | Substrate | 1 | |
Metal bindingi | 237 | Manganese | 1 | |
Binding sitei | 238 | Substrate | 1 | |
Metal bindingi | 239 | Manganese | 1 | |
Binding sitei | 342 | Substrate | 1 | |
Metal bindingi | 370 | Manganese | 1 | |
Binding sitei | 373 | Substrate | 1 | |
Binding sitei | 378 | Substrate | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: GO_Central
GO - Biological processi
- O-glycan processing Source: GO_Central
- protein O-linked glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q86SR1 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 10 Short name: GalNAc-T10 Short name: pp-GaNTase 10 Protein-UDP acetylgalactosaminyltransferase 10 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10 |
Gene namesi | Name:GALNT10 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000164574.15 |
HGNCi | HGNC:19873, GALNT10 |
MIMi | 608043, gene |
neXtProti | NX_Q86SR1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 31 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 32 – 603 | LumenalSequence analysisAdd BLAST | 572 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 55568 |
OpenTargetsi | ENSG00000164574 |
PharmGKBi | PA134870832 |
Miscellaneous databases
Pharosi | Q86SR1, Tbio |
Polymorphism and mutation databases
BioMutai | GALNT10 |
DMDMi | 51315962 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059122 | 1 – 603 | Polypeptide N-acetylgalactosaminyltransferase 10Add BLAST | 603 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 124 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 135 ↔ 365 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 146 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 356 ↔ 432 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 471 ↔ 488 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 523 ↔ 538 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 563 ↔ 578 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 593 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q86SR1 |
jPOSTi | Q86SR1 |
MassIVEi | Q86SR1 |
MaxQBi | Q86SR1 |
PaxDbi | Q86SR1 |
PeptideAtlasi | Q86SR1 |
PRIDEi | Q86SR1 |
ProteomicsDBi | 69624 [Q86SR1-1] 69625 [Q86SR1-2] 69626 [Q86SR1-3] 69627 [Q86SR1-4] |
PTM databases
GlyGeni | Q86SR1, 5 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q86SR1 |
PhosphoSitePlusi | Q86SR1 |
SwissPalmi | Q86SR1 |
Expressioni
Tissue specificityi
Widely expressed. Expressed at high level in small intestine, and at intermediate levels in stomach, pancreas, ovary, thyroid gland and spleen. Weakly expressed in other tissues.1 Publication
Gene expression databases
Bgeei | ENSG00000164574, Expressed in female gonad and 217 other tissues |
ExpressionAtlasi | Q86SR1, baseline and differential |
Genevisiblei | Q86SR1, HS |
Organism-specific databases
HPAi | ENSG00000164574, Tissue enhanced (ovary) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 120723, 10 interactors |
IntActi | Q86SR1, 4 interactors |
MINTi | Q86SR1 |
STRINGi | 9606.ENSP00000297107 |
Miscellaneous databases
RNActi | Q86SR1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q86SR1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q86SR1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 458 – 590 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 133 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 253 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 311 – 373 | Catalytic subdomain BAdd BLAST | 63 | |
Regioni | 373 – 384 | Flexible loopAdd BLAST | 12 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156690 |
HOGENOMi | CLU_013477_3_3_1 |
InParanoidi | Q86SR1 |
OMAi | NYRAQDE |
OrthoDBi | 276134at2759 |
PhylomeDBi | Q86SR1 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q86SR1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRKEKRLLQ AVALVLAALV LLPNVGLWAL YRERQPDGTP GGSGAAVAPA
60 70 80 90 100
AGQGSHSRQK KTFFLGDGQK LKDWHDKEAI RRDAQRVGNG EQGRPYPMTD
110 120 130 140 150
AERVDQAYRE NGFNIYVSDK ISLNRSLPDI RHPNCNSKRY LETLPNTSII
160 170 180 190 200
IPFHNEGWSS LLRTVHSVLN RSPPELVAEI VLVDDFSDRE HLKKPLEDYM
210 220 230 240 250
ALFPSVRILR TKKREGLIRT RMLGASVATG DVITFLDSHC EANVNWLPPL
260 270 280 290 300
LDRIARNRKT IVCPMIDVID HDDFRYETQA GDAMRGAFDW EMYYKRIPIP
310 320 330 340 350
PELQKADPSD PFESPVMAGG LFAVDRKWFW ELGGYDPGLE IWGGEQYEIS
360 370 380 390 400
FKVWMCGGRM EDIPCSRVGH IYRKYVPYKV PAGVSLARNL KRVAEVWMDE
410 420 430 440 450
YAEYIYQRRP EYRHLSAGDV AVQKKLRSSL NCKSFKWFMT KIAWDLPKFY
460 470 480 490 500
PPVEPPAAAW GEIRNVGTGL CADTKHGALG SPLRLEGCVR GRGEAAWNNM
510 520 530 540 550
QVFTFTWRED IRPGDPQHTK KFCFDAISHT SPVTLYDCHS MKGNQLWKYR
560 570 580 590 600
KDKTLYHPVS GSCMDCSESD HRIFMNTCNP SSLTQQWLFE HTNSTVLEKF
NRN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF2Z2M7 | F2Z2M7_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT10 | 202 | Annotation score: |
Sequence cautioni
The sequence BAB14676 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 518 | H → Y in BAC56890 (PubMed:12417297).Curated | 1 | |
Sequence conflicti | 518 | H → Y in BAB14676 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011207 | 1 – 329 | Missing in isoform 4. 1 PublicationAdd BLAST | 329 | |
Alternative sequenceiVSP_011209 | 190 – 251 | Missing in isoform 2. 1 PublicationAdd BLAST | 62 | |
Alternative sequenceiVSP_011208 | 330 – 352 | WELGG…EISFK → MLAWRDGELEAETSSSLFLL AMQ in isoform 4. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_011212 | 354 – 366 | WMCGG…DIPCS → SQLSRRPVLGTAS in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_011213 | 367 – 603 | Missing in isoform 3. 1 PublicationAdd BLAST | 237 | |
Alternative sequenceiVSP_011214 | 389 | N → VRT in isoform 4. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB078145 mRNA Translation: BAC56890.1 AJ505950 mRNA Translation: CAD44532.1 AK023782 mRNA Translation: BAB14676.1 Different initiation. AK127135 mRNA Translation: BAG54441.1 AL096739 mRNA Translation: CAB46378.1 AK074132 mRNA Translation: BAB84958.1 BC007224 mRNA Translation: AAH07224.2 BC072450 mRNA Translation: AAH72450.1 |
CCDSi | CCDS4325.1 [Q86SR1-1] |
PIRi | T12552 |
RefSeqi | NP_938080.1, NM_198321.3 [Q86SR1-1] |
Genome annotation databases
Ensembli | ENST00000297107; ENSP00000297107; ENSG00000164574 [Q86SR1-1] ENST00000377661; ENSP00000366889; ENSG00000164574 [Q86SR1-2] ENST00000425427; ENSP00000415210; ENSG00000164574 [Q86SR1-3] |
GeneIDi | 55568 |
KEGGi | hsa:55568 |
UCSCi | uc003lvg.2, human [Q86SR1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 10 |
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB078145 mRNA Translation: BAC56890.1 AJ505950 mRNA Translation: CAD44532.1 AK023782 mRNA Translation: BAB14676.1 Different initiation. AK127135 mRNA Translation: BAG54441.1 AL096739 mRNA Translation: CAB46378.1 AK074132 mRNA Translation: BAB84958.1 BC007224 mRNA Translation: AAH07224.2 BC072450 mRNA Translation: AAH72450.1 |
CCDSi | CCDS4325.1 [Q86SR1-1] |
PIRi | T12552 |
RefSeqi | NP_938080.1, NM_198321.3 [Q86SR1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2D7I | X-ray | 2.50 | A | 40-603 | [»] | |
2D7R | X-ray | 2.80 | A | 40-603 | [»] | |
SMRi | Q86SR1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120723, 10 interactors |
IntActi | Q86SR1, 4 interactors |
MINTi | Q86SR1 |
STRINGi | 9606.ENSP00000297107 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q86SR1, 5 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q86SR1 |
PhosphoSitePlusi | Q86SR1 |
SwissPalmi | Q86SR1 |
Polymorphism and mutation databases
BioMutai | GALNT10 |
DMDMi | 51315962 |
Proteomic databases
EPDi | Q86SR1 |
jPOSTi | Q86SR1 |
MassIVEi | Q86SR1 |
MaxQBi | Q86SR1 |
PaxDbi | Q86SR1 |
PeptideAtlasi | Q86SR1 |
PRIDEi | Q86SR1 |
ProteomicsDBi | 69624 [Q86SR1-1] 69625 [Q86SR1-2] 69626 [Q86SR1-3] 69627 [Q86SR1-4] |
Protocols and materials databases
Antibodypediai | 2029, 158 antibodies |
DNASUi | 55568 |
Genome annotation databases
Ensembli | ENST00000297107; ENSP00000297107; ENSG00000164574 [Q86SR1-1] ENST00000377661; ENSP00000366889; ENSG00000164574 [Q86SR1-2] ENST00000425427; ENSP00000415210; ENSG00000164574 [Q86SR1-3] |
GeneIDi | 55568 |
KEGGi | hsa:55568 |
UCSCi | uc003lvg.2, human [Q86SR1-1] |
Organism-specific databases
CTDi | 55568 |
DisGeNETi | 55568 |
EuPathDBi | HostDB:ENSG00000164574.15 |
GeneCardsi | GALNT10 |
HGNCi | HGNC:19873, GALNT10 |
HPAi | ENSG00000164574, Tissue enhanced (ovary) |
MIMi | 608043, gene |
neXtProti | NX_Q86SR1 |
OpenTargetsi | ENSG00000164574 |
PharmGKBi | PA134870832 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156690 |
HOGENOMi | CLU_013477_3_3_1 |
InParanoidi | Q86SR1 |
OMAi | NYRAQDE |
OrthoDBi | 276134at2759 |
PhylomeDBi | Q86SR1 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
PathwayCommonsi | Q86SR1 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 55568, 3 hits in 843 CRISPR screens |
ChiTaRSi | GALNT10, human |
EvolutionaryTracei | Q86SR1 |
GenomeRNAii | 55568 |
Pharosi | Q86SR1, Tbio |
PROi | PR:Q86SR1 |
RNActi | Q86SR1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164574, Expressed in female gonad and 217 other tissues |
ExpressionAtlasi | Q86SR1, baseline and differential |
Genevisiblei | Q86SR1, HS |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT10_HUMAN | |
Accessioni | Q86SR1Primary (citable) accession number: Q86SR1 Secondary accession number(s): B3KXC9 Q9Y4M4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families