UniProtKB - Q86SG6 (NEK8_HUMAN)
Serine/threonine-protein kinase Nek8
NEK8
Functioni
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity).
Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
By similarity1 PublicationCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 128 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- animal organ morphogenesis Source: UniProtKB
- chromosome segregation Source: GO_Central
- determination of left/right symmetry Source: Ensembl
- heart development Source: Ensembl
- regulation of hippo signaling Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q86SG6 |
SignaLinki | Q86SG6 |
SIGNORi | Q86SG6 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase Nek8 (EC:2.7.11.1)Alternative name(s): Never in mitosis A-related kinase 8 Short name: NimA-related protein kinase 8 Nima-related protein kinase 12a |
Gene namesi | Name:NEK8 Synonyms:JCK, NEK12A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13387, NEK8 |
MIMi | 609799, gene |
neXtProti | NX_Q86SG6 |
VEuPathDBi | HostDB:ENSG00000160602 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- cytoskeleton By similarity
- centrosome 1 Publication
Other locations
- cilium 1 Publication
Note: Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells.By similarity
Cytoskeleton
- centrosome Source: GO_Central
Nucleus
- nucleus Source: GO_Central
Other locations
- ciliary base Source: Ensembl
- ciliary inversin compartment Source: Ensembl
- cilium Source: CACAO
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, CytoskeletonPathology & Biotechi
Involvement in diseasei
Nephronophthisis 9 (NPHP9)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065769 | 330 | L → F in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs199962228Ensembl. | 1 | |
Natural variantiVAR_065770 | 425 | H → Y in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs118204032EnsemblClinVar. | 1 | |
Natural variantiVAR_065771 | 497 | A → P in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs146326420Ensembl. | 1 |
Renal-hepatic-pancreatic dysplasia 2 (RHPD2)1 Publication
Keywords - Diseasei
Ciliopathy, Disease variant, NephronophthisisOrganism-specific databases
DisGeNETi | 284086 |
GeneReviewsi | NEK8 |
MalaCardsi | NEK8 |
MIMi | 613824, phenotype 615415, phenotype |
OpenTargetsi | ENSG00000160602 |
Orphaneti | 93591, Infantile nephronophthisis 294415, Renal-hepatic-pancreatic dysplasia |
PharmGKBi | PA38361 |
Miscellaneous databases
Pharosi | Q86SG6, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2417353 |
Genetic variation databases
BioMutai | NEK8 |
DMDMi | 34098463 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086432 | 1 – 692 | Serine/threonine-protein kinase Nek8Add BLAST | 692 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 162 | Phosphothreonine; by autocatalysisBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q86SG6 |
MassIVEi | Q86SG6 |
PaxDbi | Q86SG6 |
PeptideAtlasi | Q86SG6 |
PRIDEi | Q86SG6 |
ProteomicsDBi | 69588 |
PTM databases
iPTMneti | Q86SG6 |
PhosphoSitePlusi | Q86SG6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000160602, Expressed in amniotic fluid and 175 other tissues |
ExpressionAtlasi | Q86SG6, baseline and differential |
Genevisiblei | Q86SG6, HS |
Organism-specific databases
HPAi | ENSG00000160602, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PKD2; may regulate PKD2 targeting to the cilium (By similarity).
Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3 (PubMed:23418306, PubMed:23793029). ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme (PubMed:23418306, PubMed:23793029).
Interacts with ANKS3 (PubMed:26188091).
By similarity3 PublicationsBinary interactionsi
Q86SG6
Protein-protein interaction databases
BioGRIDi | 129755, 37 interactors |
CORUMi | Q86SG6 |
IntActi | Q86SG6, 39 interactors |
STRINGi | 9606.ENSP00000268766 |
Chemistry databases
BindingDBi | Q86SG6 |
Miscellaneous databases
RNActi | Q86SG6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 258 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Repeati | 312 – 350 | RCC1 1Add BLAST | 39 | |
Repeati | 410 – 461 | RCC1 2Add BLAST | 52 | |
Repeati | 462 – 513 | RCC1 3Add BLAST | 52 | |
Repeati | 580 – 631 | RCC1 4Add BLAST | 52 | |
Repeati | 632 – 684 | RCC1 5Add BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 277 – 301 | DisorderedSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0589, Eukaryota |
GeneTreei | ENSGT00940000159297 |
HOGENOMi | CLU_000288_123_1_1 |
InParanoidi | Q86SG6 |
OrthoDBi | 1062377at2759 |
PhylomeDBi | Q86SG6 |
TreeFami | TF106472 |
Family and domain databases
CDDi | cd08220, STKc_Nek8, 1 hit |
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens IPR008271, Ser/Thr_kinase_AS IPR044120, STKc_Nek8 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00415, RCC1, 4 hits |
PRINTSi | PR00633, RCCNDNSATION |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50012, RCC1_3, 5 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEKYERIRVV GRGAFGIVHL CLRKADQKLV IIKQIPVEQM TKEERQAAQN
60 70 80 90 100
ECQVLKLLNH PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL
110 120 130 140 150
EEETILHFFV QILLALHHVH THLILHRDLK TQNILLDKHR MVVKIGDFGI
160 170 180 190 200
SKILSSKSKA YTVVGTPCYI SPELCEGKPY NQKSDIWALG CVLYELASLK
210 220 230 240 250
RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS LEPAQRPPLS
260 270 280 290 300
HIMAQPLCIR ALLNLHTDVG SVRMRRAEKS VAPSNTGSRT TSVRCRGIPR
310 320 330 340 350
GPVRPAIPPP LSSVYAWGGG LGTPLRLPML NTEVVQVAAG RTQKAGVTRS
360 370 380 390 400
GRLILWEAPP LGAGGGSLLP GAVEQPQPQF ISRFLEGQSG VTIKHVACGD
410 420 430 440 450
FFTACLTDRG IIMTFGSGSN GCLGHGSLTD ISQPTIVEAL LGYEMVQVAC
460 470 480 490 500
GASHVLALST ERELFAWGRG DSGRLGLGTR ESHSCPQQVP MPPGQEAQRV
510 520 530 540 550
VCGIDSSMIL TVPGQALACG SNRFNKLGLD HLSLGEEPVP HQQVEEALSF
560 570 580 590 600
TLLGSAPLDQ EPLLSIDLGT AHSAAVTASG DCYTFGSNQH GQLGTNTRRG
610 620 630 640 650
SRAPCKVQGL EGIKMAMVAC GDAFTVAIGA ESEVYSWGKG ARGRLGRRDE
660 670 680 690
DAGLPRPVQL DETHPYTVTS VSCCHGNTLL AVRSVTDEPV PP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EMF0 | K7EMF0_HUMAN | Serine/threonine-protein kinase Nek... | NEK8 | 261 | Annotation score: | ||
K7EL04 | K7EL04_HUMAN | Serine/threonine-protein kinase Nek... | NEK8 | 460 | Annotation score: | ||
K7EPD3 | K7EPD3_HUMAN | Serine/threonine-protein kinase Nek... | NEK8 | 165 | Annotation score: | ||
K7END4 | K7END4_HUMAN | Serine/threonine-protein kinase Nek... | NEK8 | 167 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065769 | 330 | L → F in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs199962228Ensembl. | 1 | |
Natural variantiVAR_065770 | 425 | H → Y in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs118204032EnsemblClinVar. | 1 | |
Natural variantiVAR_065771 | 497 | A → P in NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 PublicationCorresponds to variant dbSNP:rs146326420Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY242354 mRNA Translation: AAO88243.1 AY267371 mRNA Translation: AAP04006.1 AC010761 Genomic DNA No translation available. CH471159 Genomic DNA Translation: EAW51136.1 BC112240 mRNA Translation: AAI12241.1 BC113705 mRNA Translation: AAI13706.1 AL833909 mRNA Translation: CAD38765.1 |
CCDSi | CCDS32597.1 |
RefSeqi | NP_835464.1, NM_178170.2 |
Genome annotation databases
Ensembli | ENST00000268766; ENSP00000268766; ENSG00000160602 |
GeneIDi | 284086 |
KEGGi | hsa:284086 |
MANE-Selecti | ENST00000268766.11; ENSP00000268766.6; NM_178170.3; NP_835464.1 |
UCSCi | uc002hcp.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY242354 mRNA Translation: AAO88243.1 AY267371 mRNA Translation: AAP04006.1 AC010761 Genomic DNA No translation available. CH471159 Genomic DNA Translation: EAW51136.1 BC112240 mRNA Translation: AAI12241.1 BC113705 mRNA Translation: AAI13706.1 AL833909 mRNA Translation: CAD38765.1 |
CCDSi | CCDS32597.1 |
RefSeqi | NP_835464.1, NM_178170.2 |
3D structure databases
SMRi | Q86SG6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 129755, 37 interactors |
CORUMi | Q86SG6 |
IntActi | Q86SG6, 39 interactors |
STRINGi | 9606.ENSP00000268766 |
Chemistry databases
BindingDBi | Q86SG6 |
ChEMBLi | CHEMBL2417353 |
PTM databases
iPTMneti | Q86SG6 |
PhosphoSitePlusi | Q86SG6 |
Genetic variation databases
BioMutai | NEK8 |
DMDMi | 34098463 |
Proteomic databases
jPOSTi | Q86SG6 |
MassIVEi | Q86SG6 |
PaxDbi | Q86SG6 |
PeptideAtlasi | Q86SG6 |
PRIDEi | Q86SG6 |
ProteomicsDBi | 69588 |
Protocols and materials databases
Antibodypediai | 26547, 291 antibodies from 25 providers |
DNASUi | 284086 |
Genome annotation databases
Ensembli | ENST00000268766; ENSP00000268766; ENSG00000160602 |
GeneIDi | 284086 |
KEGGi | hsa:284086 |
MANE-Selecti | ENST00000268766.11; ENSP00000268766.6; NM_178170.3; NP_835464.1 |
UCSCi | uc002hcp.4, human |
Organism-specific databases
CTDi | 284086 |
DisGeNETi | 284086 |
GeneCardsi | NEK8 |
GeneReviewsi | NEK8 |
HGNCi | HGNC:13387, NEK8 |
HPAi | ENSG00000160602, Low tissue specificity |
MalaCardsi | NEK8 |
MIMi | 609799, gene 613824, phenotype 615415, phenotype |
neXtProti | NX_Q86SG6 |
OpenTargetsi | ENSG00000160602 |
Orphaneti | 93591, Infantile nephronophthisis 294415, Renal-hepatic-pancreatic dysplasia |
PharmGKBi | PA38361 |
VEuPathDBi | HostDB:ENSG00000160602 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0589, Eukaryota |
GeneTreei | ENSGT00940000159297 |
HOGENOMi | CLU_000288_123_1_1 |
InParanoidi | Q86SG6 |
OrthoDBi | 1062377at2759 |
PhylomeDBi | Q86SG6 |
TreeFami | TF106472 |
Enzyme and pathway databases
PathwayCommonsi | Q86SG6 |
SignaLinki | Q86SG6 |
SIGNORi | Q86SG6 |
Miscellaneous databases
BioGRID-ORCSi | 284086, 13 hits in 1078 CRISPR screens |
GeneWikii | NEK8 |
GenomeRNAii | 284086 |
Pharosi | Q86SG6, Tbio |
PROi | PR:Q86SG6 |
RNActi | Q86SG6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000160602, Expressed in amniotic fluid and 175 other tissues |
ExpressionAtlasi | Q86SG6, baseline and differential |
Genevisiblei | Q86SG6, HS |
Family and domain databases
CDDi | cd08220, STKc_Nek8, 1 hit |
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens IPR008271, Ser/Thr_kinase_AS IPR044120, STKc_Nek8 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00415, RCC1, 4 hits |
PRINTSi | PR00633, RCCNDNSATION |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50012, RCC1_3, 5 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NEK8_HUMAN | |
Accessioni | Q86SG6Primary (citable) accession number: Q86SG6 Secondary accession number(s): A6NIC5 Q8NDH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families