UniProtKB - Q86SF2 (GALT7_HUMAN)
Protein
N-acetylgalactosaminyltransferase 7
Gene
GALNT7
Organism
Homo sapiens (Human)
Status
Functioni
Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.2 Publications
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP2 PublicationsEC:2.4.1.412 Publications
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP2 PublicationsEC:2.4.1.412 Publications
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 247 | SubstrateBy similarity | 1 | |
Binding sitei | 277 | SubstrateBy similarity | 1 | |
Metal bindingi | 301 | ManganeseBy similarity | 1 | |
Metal bindingi | 303 | ManganeseBy similarity | 1 | |
Binding sitei | 412 | SubstrateBy similarity | 1 | |
Metal bindingi | 440 | ManganeseBy similarity | 1 | |
Binding sitei | 443 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: ProtInc
GO - Biological processi
- carbohydrate metabolic process Source: ProtInc
- O-glycan processing Source: Reactome
- protein O-linked glycosylation Source: ProtInc
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q86SF2 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylgalactosaminyltransferase 7 (EC:2.4.1.412 Publications)Alternative name(s): Polypeptide GalNAc transferase 7 Short name: GalNAc-T7 Short name: pp-GaNTase 7 Protein-UDP acetylgalactosaminyltransferase 7 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 |
Gene namesi | Name:GALNT7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000109586.11 |
HGNCi | HGNC:4129, GALNT7 |
MIMi | 605005, gene |
neXtProti | NX_Q86SF2 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 30 – 657 | LumenalSequence analysisAdd BLAST | 628 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 51809 |
OpenTargetsi | ENSG00000109586 |
PharmGKBi | PA28542 |
Miscellaneous databases
Pharosi | Q86SF2, Tbio |
Polymorphism and mutation databases
BioMutai | GALNT7 |
DMDMi | 51315961 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059116 | 1 – 657 | N-acetylgalactosaminyltransferase 7Add BLAST | 657 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 197 ↔ 435 | PROSITE-ProRule annotation | ||
Disulfide bondi | 426 ↔ 507 | PROSITE-ProRule annotation | ||
Disulfide bondi | 545 ↔ 562 | PROSITE-ProRule annotation | ||
Disulfide bondi | 585 ↔ 600 | PROSITE-ProRule annotation | ||
Disulfide bondi | 625 ↔ 640 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | Q86SF2 |
jPOSTi | Q86SF2 |
MassIVEi | Q86SF2 |
MaxQBi | Q86SF2 |
PaxDbi | Q86SF2 |
PeptideAtlasi | Q86SF2 |
PRIDEi | Q86SF2 |
ProteomicsDBi | 69579 |
PTM databases
iPTMneti | Q86SF2 |
PhosphoSitePlusi | Q86SF2 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in uterus, retina, kidney, small intestine, omentum, stomach and CNS.1 Publication
Gene expression databases
Bgeei | ENSG00000109586, Expressed in thymus and 234 other tissues |
ExpressionAtlasi | Q86SF2, baseline and differential |
Genevisiblei | Q86SF2, HS |
Organism-specific databases
HPAi | ENSG00000109586, Tissue enhanced (lymphoid) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 119735, 37 interactors |
IntActi | Q86SF2, 21 interactors |
MINTi | Q86SF2 |
STRINGi | 9606.ENSP00000265000 |
Miscellaneous databases
RNActi | Q86SF2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q86SF2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 532 – 652 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 121 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 206 – 317 | Catalytic subdomain AAdd BLAST | 112 | |
Regioni | 381 – 443 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3737, Eukaryota |
GeneTreei | ENSGT00940000158105 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q86SF2 |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q86SF2 |
TreeFami | TF352176 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q86SF2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLKIGFILR SLLVVGSFLG LVVLWSSLTP RPDDPSPLSR MREDRDVNDP
60 70 80 90 100
MPNRGGNGLA PGEDRFKPVV PWPHVEGVEV DLESIRRINK AKNEQEHHAG
110 120 130 140 150
GDSQKDIMQR QYLTFKPQTF TYHDPVLRPG ILGNFEPKEP EPPGVVGGPG
160 170 180 190 200
EKAKPLVLGP EFKQAIQASI KEFGFNMVAS DMISLDRSVN DLRQEECKYW
210 220 230 240 250
HYDENLLTSS VVIVFHNEGW STLMRTVHSV IKRTPRKYLA EIVLIDDFSN
260 270 280 290 300
KEHLKEKLDE YIKLWNGLVK VFRNERREGL IQARSIGAQK AKLGQVLIYL
310 320 330 340 350
DAHCEVAVNW YAPLVAPISK DRTICTVPLI DVINGNTYEI IPQGGGDEDG
360 370 380 390 400
YARGAWDWSM LWKRVPLTPQ EKRLRKTKTE PYRSPAMAGG LFAIEREFFF
410 420 430 440 450
ELGLYDPGLQ IWGGENFEIS YKIWQCGGKL LFVPCSRVGH IYRLEGWQGN
460 470 480 490 500
PPPIYVGSSP TLKNYVRVVE VWWDEYKDYF YASRPESQAL PYGDISELKK
510 520 530 540 550
FREDHNCKSF KWFMEEIAYD ITSHYPLPPK NVDWGEIRGF ETAYCIDSMG
560 570 580 590 600
KTNGGFVELG PCHRMGGNQL FRINEANQLM QYDQCLTKGA DGSKVMITHC
610 620 630 640 650
NLNEFKEWQY FKNLHRFTHI PSGKCLDRSE VLHQVFISNC DSSKTTQKWE
MNNIHSV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YAH3 | H0YAH3_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT7 | 454 | Annotation score: | ||
E9PBY3 | E9PBY3_HUMAN | N-acetylgalactosaminyltransferase 7 | GALNT7 | 383 | Annotation score: | ||
H0YAK0 | H0YAK0_HUMAN | N-acetylgalactosaminyltransferase 7 | GALNT7 | 199 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | S → N in CAB60270 (PubMed:10544240).Curated | 1 | |
Sequence conflicti | 392 | F → C in CAB60270 (PubMed:10544240).Curated | 1 | |
Sequence conflicti | 408 | G → S in CAB60270 (PubMed:10544240).Curated | 1 | |
Sequence conflicti | 508 | K → Q in CAB60270 (PubMed:10544240).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002744 mRNA Translation: CAB60270.1 AK075488 mRNA Translation: BAG52155.1 CH471056 Genomic DNA Translation: EAX04761.1 BC035303 mRNA Translation: AAH35303.1 BC046129 mRNA Translation: AAH46129.1 BC047468 mRNA Translation: AAH47468.1 |
CCDSi | CCDS3815.1 |
RefSeqi | NP_059119.2, NM_017423.2 |
Genome annotation databases
Ensembli | ENST00000265000; ENSP00000265000; ENSG00000109586 |
GeneIDi | 51809 |
KEGGi | hsa:51809 |
UCSCi | uc003isz.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase N-acetylgalactosaminyltransferase 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002744 mRNA Translation: CAB60270.1 AK075488 mRNA Translation: BAG52155.1 CH471056 Genomic DNA Translation: EAX04761.1 BC035303 mRNA Translation: AAH35303.1 BC046129 mRNA Translation: AAH46129.1 BC047468 mRNA Translation: AAH47468.1 |
CCDSi | CCDS3815.1 |
RefSeqi | NP_059119.2, NM_017423.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6IWQ | X-ray | 2.95 | A/B/C/D/E/F | 61-657 | [»] | |
6IWR | X-ray | 2.60 | A/B/C/D/E/F | 61-657 | [»] | |
SMRi | Q86SF2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119735, 37 interactors |
IntActi | Q86SF2, 21 interactors |
MINTi | Q86SF2 |
STRINGi | 9606.ENSP00000265000 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
iPTMneti | Q86SF2 |
PhosphoSitePlusi | Q86SF2 |
Polymorphism and mutation databases
BioMutai | GALNT7 |
DMDMi | 51315961 |
Proteomic databases
EPDi | Q86SF2 |
jPOSTi | Q86SF2 |
MassIVEi | Q86SF2 |
MaxQBi | Q86SF2 |
PaxDbi | Q86SF2 |
PeptideAtlasi | Q86SF2 |
PRIDEi | Q86SF2 |
ProteomicsDBi | 69579 |
Protocols and materials databases
Antibodypediai | 28517, 102 antibodies |
DNASUi | 51809 |
Genome annotation databases
Ensembli | ENST00000265000; ENSP00000265000; ENSG00000109586 |
GeneIDi | 51809 |
KEGGi | hsa:51809 |
UCSCi | uc003isz.4, human |
Organism-specific databases
CTDi | 51809 |
DisGeNETi | 51809 |
EuPathDBi | HostDB:ENSG00000109586.11 |
GeneCardsi | GALNT7 |
HGNCi | HGNC:4129, GALNT7 |
HPAi | ENSG00000109586, Tissue enhanced (lymphoid) |
MIMi | 605005, gene |
neXtProti | NX_Q86SF2 |
OpenTargetsi | ENSG00000109586 |
PharmGKBi | PA28542 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3737, Eukaryota |
GeneTreei | ENSGT00940000158105 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q86SF2 |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q86SF2 |
TreeFami | TF352176 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q86SF2 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 51809, 4 hits in 846 CRISPR screens |
ChiTaRSi | GALNT7, human |
GenomeRNAii | 51809 |
Pharosi | Q86SF2, Tbio |
PROi | PR:Q86SF2 |
RNActi | Q86SF2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000109586, Expressed in thymus and 234 other tissues |
ExpressionAtlasi | Q86SF2, baseline and differential |
Genevisiblei | Q86SF2, HS |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT7_HUMAN | |
Accessioni | Q86SF2Primary (citable) accession number: Q86SF2 Secondary accession number(s): B3KQU3, Q7Z5W7, Q9UJ28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families