UniProtKB - Q86PC9 (TULP_DROME)
Protein
Protein king tubby
Gene
ktub
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Functions in regulating protein trafficking, retinal maintenance and lipid storage (PubMed:23228091, PubMed:23355467, PubMed:24068974, PubMed:26723017). Protects photoreceptor cells R1 to R6 against light-induced retinal degeneration by stimulating norpA-mediated endocytosis of the rhodopsin ninaE (Rh1) (PubMed:23228091). In the auditory receptor neurons, functions as a cilia trafficking regulator of various transient receptor potential (TRP) channel components including iav and nompC (PubMed:24068974, PubMed:26723017). Likely to deliver pre-ciliary vesicles containing membrane proteins such as iav and nompC to the intraflagellar transport complex (IFT) at the cilia base (PubMed:24068974, PubMed:26723017). Plays a role in the inhibition of fat storage (PubMed:23355467).4 Publications
GO - Molecular functioni
- lipid binding Source: UniProtKB-KW
GO - Biological processi
- deactivation of rhodopsin mediated signaling Source: FlyBase
- endocytosis Source: FlyBase
- glucose homeostasis Source: FlyBase
- protein localization to cilium Source: FlyBase
- response to sucrose Source: FlyBase
- sensory perception of sound Source: FlyBase
- triglyceride homeostasis Source: FlyBase
Keywordsi
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-DME-5610787, Hedgehog 'off' state |
Names & Taxonomyi
Protein namesi | Recommended name: Protein king tubby1 Publication |
Gene namesi | Name:ktub Synonyms:Tulp ORF Names:CG9398 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0015721, ktub |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Nucleus
- Nucleus 3 Publications
Plasma membrane
- cilium membrane 2 Publications; Peripheral membrane protein 2 Publications
Note: Detected in the cytoplasm and nucleus of the chordotonal neurons (PubMed:12204260, PubMed:24068974). PtdIns 4,5-P2 binds to and regulates its localization to the ciliary base (PubMed:26723017). In the dark, localizes mainly to the rhabdomere domain of photoreceptor cells R1 to R6 (PubMed:23228091). When dark-reared flies are moved into the light, the protein translocates to the cytoplasm (PubMed:23228091). Under normal conditions (12 hr light/12 hr dark), localized mainly in the nucleus of photoreceptor cells with minor staining in the cytoplasm (PubMed:23228091).4 Publications
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- ciliary membrane Source: UniProtKB-SubCell
Other locations
- cilium Source: FlyBase
- cytoplasm Source: UniProtKB
- neuronal cell body Source: FlyBase
- rhabdomere Source: FlyBase
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Viable and fertile however flies display a decreased climbing index and extracellular sound-evoked potentials are completely abolished (PubMed:24068974). This hearing defect is likely due to the abnormal localization of the two transient receptor potential channels iav and nompC, as well as eys within the cilia (PubMed:24068974). There is no localization of iav to the proximal cilia, and nompc which is typically found in the distal cilia, is localized to the proximal cilia (PubMed:24068974). Also eys displays a much broader expression pattern (PubMed:24068974). RNAi-mediated knockdown in the fat body of larvae fed a high sugar diet, results in an increase in body weight, an increase in triglyceride accumulation and a decrease in hemolymph glucose levels (PubMed:23355467).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 – 26 | QKR → AAA in Muta; reduced iav expression in the cilia but is not required for lav or nompC localization in the cilia. 1 Publication | 3 | |
Mutagenesisi | 292 – 294 | KLR → ALA in Mutb; reduced iav expression in the cilia but is not required for lav or nompC localization in the cilia. Decrease in extracellular sound-evoked potentials. Sounds-evoked potentials, and localization of ktub, iav and nompC is partially restored in a ktub and INPP5E double mutant background. 2 Publications | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000400838 | 1 – 460 | Protein king tubbyAdd BLAST | 460 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 153 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q86PC9 |
PRIDEi | Q86PC9 |
PTM databases
iPTMneti | Q86PC9 |
Expressioni
Tissue specificityi
Detected in sensory neurons which have a ciliary structure such as the chordotonal neurons, Orco-expressing olfactory receptor neurons, labellar gustatory receptor neurons and in the femoral chordotonal organ (at protein level) (PubMed:24068974, PubMed:26723017). In the chordotonal neurons of the Johnston's organ expressed in the proximal to distal cilia, with lower levels of expression in the distal portion (at protein level) (PubMed:24068974). Also detected in the salivary glands and antenna (at protein level) (PubMed:24068974). Expressed in photoreceptor cells (at protein level) (PubMed:23228091). At stage 9 expression is detected in a subset of neuroblasts (PubMed:12204260). By stage 12 expression is found in both the CNS and PNS (PubMed:12204260). In late-stage embryos, expression persists in the CNS and PNS with more abundant expression in the antennal-maxillary sensory neurons and in bilateral groups of cells in the brain (PubMed:12204260).4 Publications
Developmental stagei
Most abundant at 2-8 hours of embryonic development.1 Publication
Gene expression databases
Bgeei | FBgn0015721, Expressed in embryo and 47 other tissues |
Genevisiblei | Q86PC9, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 63037, 20 interactors |
IntActi | Q86PC9, 14 interactors |
STRINGi | 7227.FBpp0088961 |
Family & Domainsi
Domaini
The C-terminus is important for mediating light-induced rhodopsin endocytosis.1 Publication
Sequence similaritiesi
Belongs to the TUB family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG2502, Eukaryota |
GeneTreei | ENSGT00940000170687 |
HOGENOMi | CLU_028236_1_1_1 |
InParanoidi | Q86PC9 |
OMAi | YKCRIAR |
PhylomeDBi | Q86PC9 |
Family and domain databases
Gene3Di | 3.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR025659, Tubby-like_C IPR000007, Tubby_C IPR018066, Tubby_C_CS |
Pfami | View protein in Pfam PF01167, Tub, 1 hit |
PRINTSi | PR01573, SUPERTUBBY |
SUPFAMi | SSF54518, SSF54518, 1 hit |
PROSITEi | View protein in PROSITE PS01200, TUB_1, 1 hit PS01201, TUB_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform B1 Publication (identifier: Q86PC9-1) [UniParc]FASTAAdd to basket
Also known as: TULP-L1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGINSRNQK MEQQRQLMEA YIRQKRASPG MVQASDLQIN RPMSGMRSNS
60 70 80 90 100
RELHAYDGPM QFISSPQNPD QILTNGSPGG INPVAMNTSR NHSNNMRSLS
110 120 130 140 150
TINQEADLIE EISSHELEDE ESSPVTVIEQ HQQSASHSAN STQSQKPRAR
160 170 180 190 200
QHSFSDNLDE DDYTNRNVAG AAPVRPAGMA SSPYKDATLD GSSNGTGNGT
210 220 230 240 250
GGESEGDVIG NIDQFVMQPA PQGVLYKCRI TRDRKGMDRG LFPIYYLHLE
260 270 280 290 300
RDYGKKIFLL GGRKRKKSKT SNYIVSCDPT DLSRNADGFC GKLRSNVFGT
310 320 330 340 350
SFTVFDNGNK ESTESPRLDL AVIIYDTNIL GFKGPRNMTV ILPGMTEDDQ
360 370 380 390 400
RVKISSADPK QQGILDLWKM KNMDNIVELH NKTPVWNDET QSYVLNFHGR
410 420 430 440 450
VTQASVKNFQ LVHDSDPEYI VMQFGRTSED VFTMDYRYPL CAMQAFAIAL
460
SSFDGKIACE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | G → A in AAM91018 (PubMed:12204260).Curated | 1 | |
Sequence conflicti | 182 | S → P in AAM91018 (PubMed:12204260).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040028 | 1 – 17 | Missing in isoform A. 2 PublicationsAdd BLAST | 17 | |
Alternative sequenceiVSP_058159 | 105 | E → EGKWSYTPRK in isoform C. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF527823 mRNA Translation: AAM91018.1 AE013599 Genomic DNA Translation: AAF46675.2 AE013599 Genomic DNA Translation: AAS64753.1 AY060625 mRNA Translation: AAL28173.1 BT003201 mRNA Translation: AAO24956.1 |
RefSeqi | NP_611549.2, NM_137705.3 [Q86PC9-3] NP_995911.1, NM_206189.2 [Q86PC9-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0089600; FBpp0088961; FBgn0015721 [Q86PC9-1] FBtr0342842; FBpp0309666; FBgn0015721 [Q86PC9-3] |
GeneIDi | 37400 |
KEGGi | dme:Dmel_CG9398 |
UCSCi | CG9398-RA, d. melanogaster CG9398-RB, d. melanogaster [Q86PC9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF527823 mRNA Translation: AAM91018.1 AE013599 Genomic DNA Translation: AAF46675.2 AE013599 Genomic DNA Translation: AAS64753.1 AY060625 mRNA Translation: AAL28173.1 BT003201 mRNA Translation: AAO24956.1 |
RefSeqi | NP_611549.2, NM_137705.3 [Q86PC9-3] NP_995911.1, NM_206189.2 [Q86PC9-1] |
3D structure databases
SMRi | Q86PC9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63037, 20 interactors |
IntActi | Q86PC9, 14 interactors |
STRINGi | 7227.FBpp0088961 |
PTM databases
iPTMneti | Q86PC9 |
Proteomic databases
PaxDbi | Q86PC9 |
PRIDEi | Q86PC9 |
Genome annotation databases
EnsemblMetazoai | FBtr0089600; FBpp0088961; FBgn0015721 [Q86PC9-1] FBtr0342842; FBpp0309666; FBgn0015721 [Q86PC9-3] |
GeneIDi | 37400 |
KEGGi | dme:Dmel_CG9398 |
UCSCi | CG9398-RA, d. melanogaster CG9398-RB, d. melanogaster [Q86PC9-1] |
Organism-specific databases
CTDi | 37400 |
FlyBasei | FBgn0015721, ktub |
Phylogenomic databases
eggNOGi | KOG2502, Eukaryota |
GeneTreei | ENSGT00940000170687 |
HOGENOMi | CLU_028236_1_1_1 |
InParanoidi | Q86PC9 |
OMAi | YKCRIAR |
PhylomeDBi | Q86PC9 |
Enzyme and pathway databases
Reactomei | R-DME-5610787, Hedgehog 'off' state |
Miscellaneous databases
BioGRID-ORCSi | 37400, 0 hits in 3 CRISPR screens |
ChiTaRSi | ktub, fly |
GenomeRNAii | 37400 |
PROi | PR:Q86PC9 |
Gene expression databases
Bgeei | FBgn0015721, Expressed in embryo and 47 other tissues |
Genevisiblei | Q86PC9, DM |
Family and domain databases
Gene3Di | 3.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR025659, Tubby-like_C IPR000007, Tubby_C IPR018066, Tubby_C_CS |
Pfami | View protein in Pfam PF01167, Tub, 1 hit |
PRINTSi | PR01573, SUPERTUBBY |
SUPFAMi | SSF54518, SSF54518, 1 hit |
PROSITEi | View protein in PROSITE PS01200, TUB_1, 1 hit PS01201, TUB_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TULP_DROME | |
Accessioni | Q86PC9Primary (citable) accession number: Q86PC9 Secondary accession number(s): Q8MUB4, Q9W2L5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2010 |
Last sequence update: | June 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families