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Protein

Receptor-type guanylate cyclase gcy-28

Gene

gcy-28

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanylate cyclase involved in the production of the second messenger cGMP (PubMed:9188508). Regulates olfactory perception in AWC sensory neurons although may not be involved in the primary sensory transduction steps (PubMed:18817734).2 Publications
Isoforms c: Regulates sensory integration of conflicting sensory cues in AIA interneurons (PubMed:21414922).
Isoform d: Regulates sensory integration of conflicting sensory cues in AIA interneurons (PubMed:21414922).

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei756ATPPROSITE-ProRule annotation1
Metal bindingi1091Magnesium 1PROSITE-ProRule annotation1
Metal bindingi1091Magnesium 2PROSITE-ProRule annotation1
Metal bindingi1092Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi1135Magnesium 1PROSITE-ProRule annotation1
Metal bindingi1135Magnesium 2PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi723 – 731ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: WormBase
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: InterPro

GO - Biological processi

  • chemotaxis Source: WormBase
  • intracellular signal transduction Source: InterPro
  • olfactory behavior Source: WormBase
  • sensory perception of chemical stimulus Source: UniProtKB
  • sensory processing Source: UniProtKB
  • signal transduction Source: GO_Central

Keywordsi

Molecular functionLyase, Receptor
Biological processcGMP biosynthesis, Chemotaxis
LigandATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type guanylate cyclase gcy-28Curated (EC:4.6.1.21 Publication)
Alternative name(s):
GCY-X11 Publication
Gene namesi
Name:gcy-28Imported
ORF Names:T01A4.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiT01A4.1a ; CE33582 ; WBGene00020131 ; gcy-28
T01A4.1b ; CE25977 ; WBGene00020131 ; gcy-28
T01A4.1c ; CE33583 ; WBGene00020131 ; gcy-28
T01A4.1d ; CE43478 ; WBGene00020131 ; gcy-28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 515ExtracellularSequence analysisAdd BLAST497
Transmembranei516 – 536HelicalSequence analysisAdd BLAST21
Topological domaini537 – 1276CytoplasmicSequence analysisAdd BLAST740

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1204F → L in ky713; loss of chemotaxis response to butanone. Defect in sensory integration when confronted to two opposing environmental chemicals. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000043329519 – 1276Receptor-type guanylate cyclase gcy-28Sequence analysisAdd BLAST1258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi87N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi196N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi338N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi384N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi387N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi414N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi428N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi444N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ86GV3
PaxDbiQ86GV3
PeptideAtlasiQ86GV3

Expressioni

Tissue specificityi

Expressed in head neurons, ventral cord and tail neurons, body wall muscle, hypodermis, somatic gonad and intestine (PubMed:16547101). Isoform d is expressed specifically in AIA interneurons (PubMed:21414922).2 Publications

Gene expression databases

BgeeiWBGene00020131
ExpressionAtlasiQ86GV3 baseline and differential

Interactioni

Protein-protein interaction databases

IntActiQ86GV3, 1 interactor
STRINGi6239.T01A4.1c

Structurei

3D structure databases

ProteinModelPortaliQ86GV3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini717 – 1013Protein kinasePROSITE-ProRule annotationAdd BLAST297
Domaini1086 – 1215Guanylate cyclasePROSITE-ProRule annotationAdd BLAST130

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1017 – 1063Sequence analysisAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 40Poly-ProSequence analysis8

Domaini

The protein kinase domain is predicted to be catalytically inactive.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000293307
InParanoidiQ86GV3
KOiK12323
OMAiREDPSML
OrthoDBiEOG091G02QS
PhylomeDBiQ86GV3

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR001828 ANF_lig-bd_rcpt
IPR001170 ANPR/GUC
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00255 NATPEPTIDER
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform cImported (identifier: Q86GV3-1) [UniParc]FASTAAdd to basket
Also known as: GCY-X11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRWLTLLSC ILLTALHGNI VEDVGAAQQA SYPPPPPPFP FNVIVILPKR
60 70 80 90 100
ESTYDNFGMT LQKAMPVIDI AVQEVIKAKK LPPGWINLTY WDSRLYEDIL
110 120 130 140 150
LAERHATVGV IQAYCEHRLD AILGFADNYG LATVTKVTAG LNGGIPILTT
160 170 180 190 200
SGMPSLLNSK KEYPFLTRMQ GSYRLLADSM YQLIAYHDED SVSKSNSSLN
210 220 230 240 250
YLNLIFFYHD KRRAVNRVIA QDESQETGAT SSHCYFSLYA IKRYFTEKSK
260 270 280 290 300
TFKREWALNT PQFPFDEDLV IERETFKQWL REISLQSNVI ILCASPDTVR
310 320 330 340 350
EIMLAAHDLG MATSGEYVFI NIDVSTGSHA EQPWIRANDT NNEENEKAKE
360 370 380 390 400
AYRALKTISL RRSDLDEYKN FELRVKERAD QKYNYTNITG KDYEMNNFIS
410 420 430 440 450
AFYDAVLLYA IALNETIQSG LDPRNGHNIT SRMWGRTFVG ITGNVSIDHN
460 470 480 490 500
GDRYSDYSLL DLDPVQNRFV EVAYYSGASN QLKTVGQLHW VGGKPPTDLP
510 520 530 540 550
ICGYDKSKCP GYPLHVYLLM GSFLLILVLV GLFIFFWRRY KLEQELAAMS
560 570 580 590 600
WKIRWEELDG EESQKKNEKK KAKKRKNHND YLPESDPLLR STSRSSVNSD
610 620 630 640 650
KFDEDSLIPI RFRLRSSSSG TTRKISAMID RKLSIFTRKK STPPSESQKN
660 670 680 690 700
GGLTPNSLQK AENGDCSPIN EVQFRLPLND RRVSSPSSEA TRKKNSNEED
710 720 730 740 750
PENGAKKSLS LKNRKLSFGM VSFKSGSGGS VETIAQNNTQ IYTKTAIFKG
760 770 780 790 800
VVVAIKKLNI DPKKYPRLDL SRAQLMELKK MKDLQHDHIT RFTGACIDFP
810 820 830 840 850
HYCVVTEYCP KGSLEDILEN EKIELDKLMK YSLLHDLVKG LFFLHNSEIR
860 870 880 890 900
SHGRLKSSNC VVDSRFVLKV TDFGLHRLHC LEEINLEEIG EHAYYKKMLW
910 920 930 940 950
TAPELLRDSN APPMGTQKGD IYSFAIILHE MMFRKGVFAL ENEDLSPNEI
960 970 980 990 1000
VQRVRKPVSE DQEPLRPWVS ETGEGEGDDA LNDTLLSLMV ACWSEDPHER
1010 1020 1030 1040 1050
PEVSSVRKAV RSLNRDNETS NLVDNLLKRM EQYANNLEGL VEERTQEYLA
1060 1070 1080 1090 1100
EKKKVEELLH QLLPPAIADT LIAGRAVQAE SYDCVTIYFS DIVGFTSLSS
1110 1120 1130 1140 1150
QSTPMQVVTL LNDLYLAFDG VVDNFKVYKV ETIGDAYMVV SGLPERRDDH
1160 1170 1180 1190 1200
ANQIAQMSLS LLHKVKNFVI RHRPHEQLKL RIGMHSGSVV AGVVGSKMPR
1210 1220 1230 1240 1250
YCLFGDTVNT SSRMESNGLP LKIHVSQQTY DILMQEAGFK LELRGSVEMK
1260 1270
GKGMQTTYWL RGYKDVEIPD FGEEFA
Length:1,276
Mass (Da):144,989
Last modified:June 1, 2003 - v1
Checksum:i4941A68DD206E547
GO
Isoform aImported (identifier: Q86GV3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: MLRWLTLLSC...SQETGATSSH → MILSDVLLLS...RGPRNKGRSE
     239-289: YAIKRYFTEK...LREISLQSNV → SAIKNLIMNN...LSEASVMSNL
     510-510: P → PQ
     602-614: Missing.

Show »
Length:1,217
Mass (Da):137,811
Checksum:iC42F108F4C8CE46E
GO
Isoform bImported (identifier: Q86GV3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-627: Missing.
     1220-1264: PLKIHVSQQT...QTTYWLRGYK → RMFWNFSLYK...EGKKMVTEFN
     1265-1276: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:637
Mass (Da):72,480
Checksum:i0981DF05AEB20474
GO
Isoform dImported (identifier: Q86GV3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-287: MLRWLTLLSC...QWLREISLQS → MWINSTRTLI...NYLRASSNMA
     510-510: P → PQ
     602-614: Missing.

Show »
Length:1,238
Mass (Da):140,785
Checksum:iFF731DBFB411D372
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0577101 – 627Missing in isoform b. CuratedAdd BLAST627
Alternative sequenceiVSP_0577111 – 287MLRWL…ISLQS → MWINSTRTLIFNHFVRIVLV LLLSHEVVTKSNRKFEEISV KHPIHLVIPLPDDDDFSDGK NPFLFSSHKVKPLADLALER VYREGILPNNSVNLIYRDSK LSDAIGPNVAVEQLLRKQID CIIGYAYGYALAPVARMSPY WKNGIPIITPIGLTMSLDDK REYQLMTRINSPYKVVSSAV STLFNTYKWKRHIFMFHHAK APSVAVGECFLLMASLQHPL RKVMEMQHNFFTFNEDHGAN ISKAERHNQFRNYLRASSNM A in isoform d. CuratedAdd BLAST287
Alternative sequenceiVSP_0577121 – 233MLRWL…ATSSH → MILSDVLLLSLVISSSSSSS HQHSLLPIDIVVGLPIEEGD RGKNPFLLTLAKSKPVFDVA LQDVYHLRILPYGSLKVTFE NSALSDAVGPQKMIEHYCNK TVDAIMGLPYVYALAPVARI SKFWGQGVPVFTTTALVDEL GDRNEFPLLTRMMGSYKSLG KLVTRIAERFEWQHYFFMFN DEVARGPRNKGRSE in isoform a. CuratedAdd BLAST233
Alternative sequenceiVSP_057713239 – 289YAIKR…LQSNV → SAIKNLIMNNKTSTWNVKMF SEFEADRLQYRALLSEASVM SNL in isoform a. CuratedAdd BLAST51
Alternative sequenceiVSP_057714510P → PQ in isoform a and isoform d. Curated1
Alternative sequenceiVSP_057715602 – 614Missing in isoform a and isoform d. CuratedAdd BLAST13
Alternative sequenceiVSP_0577161220 – 1264PLKIH…LRGYK → RMFWNFSLYKIYKFKVFKNK IELDFLKNEKESIEFEGKKM VTEFN in isoform b. CuratedAdd BLAST45
Alternative sequenceiVSP_0577171265 – 1276Missing in isoform b. CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081694 Genomic DNA Translation: CCD73355.1
FO081694 Genomic DNA Translation: CCD73353.1
FO081694 Genomic DNA Translation: CCD73354.1
FO081694 Genomic DNA Translation: CCD73356.1
RefSeqiNP_001021600.1, NM_001026429.2 [Q86GV3-1]
NP_001249628.1, NM_001262699.1 [Q86GV3-4]
NP_491379.3, NM_058978.3 [Q86GV3-2]
NP_491380.1, NM_058979.1 [Q86GV3-3]
UniGeneiCel.18007

Genome annotation databases

EnsemblMetazoaiT01A4.1c; T01A4.1c; WBGene00020131 [Q86GV3-1]
GeneIDi172051
KEGGicel:CELE_T01A4.1
UCSCiT01A4.1c c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGCY28_CAEEL
AccessioniPrimary (citable) accession number: Q86GV3
Secondary accession number(s): H2L0J9, Q965F7, Q965F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: June 1, 2003
Last modified: July 18, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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