UniProtKB - Q86B44 (Q86B44_DROME)
Protein
Glutathione synthetase
Gene
Gss2
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalytic activityi
- ATP + glycine + L-γ-glutamyl-L-cysteine = ADP + glutathione + H+ + phosphateUniRule annotationARBA annotationEC:6.3.2.3UniRule annotationARBA annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
: glutathione biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotationARBA annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Glutamate--cysteine ligase (Gclc), Glutamate--cysteine ligase (Gclc), GCS light chain (Gclm)
- Glutathione synthetase, Glutathione synthetase (Gss2), Glutathione synthetase, Glutathione synthetase (Gss2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 296 | SubstrateUniRule annotation | 1 | |
Binding sitei | 384 | ATPUniRule annotation | 1 | |
Metal bindingi | 446 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 453 | ATPUniRule annotation | 1 | |
Binding sitei | 504 | ATPUniRule annotation | 1 | |
Binding sitei | 529 | SubstrateUniRule annotation | 1 | |
Binding sitei | 531 | ATPUniRule annotation | 1 | |
Binding sitei | 537 | ATP; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 442 – 451 | ATPUniRule annotation | 10 | |
Nucleotide bindingi | 475 – 478 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glutathione binding Source: GO_Central
- glutathione synthase activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
- protein homodimerization activity Source: UniProtKB-UniRule
GO - Biological processi
- detoxification of arsenic-containing substance Source: FlyBase
Keywordsi
Molecular function | LigaseUniRule annotationImportedARBA annotation |
Biological process | Glutathione biosynthesisUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotationARBA annotation, MagnesiumUniRule annotationARBA annotation, Metal-bindingUniRule annotationARBA annotation, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)Short name: GSH-SUniRule annotation |
Gene namesi | Name:Gss2Imported Synonyms:32495Imported, 6835Imported, CG32497Imported, CG33065Imported, Dmel\CG32495Imported, Dmel\CG6835Imported, GSImported, GSSImported, gssImported, Gss1Imported ORF Names:CG32495Imported, CG6835Imported, Dmel_CG32495Imported, Dmel_CG6835Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0030882, Gss1 FBgn0052495, Gss2 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Interactioni
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB-UniRule
Protein-protein interaction databases
IntActi | Q86B44, 2 interactors |
STRINGi | 7227.FBpp0290197 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 281 – 381 | GSH_synthaseInterPro annotationAdd BLAST | 101 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 176 – 202 | DisorderedSequence analysisAdd BLAST | 27 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 184 – 202 | PolyampholyteSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
GeneTreei | ENSGT00390000013764 |
HOGENOMi | CLU_025152_2_1_1 |
OrthoDBi | 1189955at2759 |
Family and domain databases
Gene3Di | 1.10.1080.10, 2 hits 3.30.1490.50, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 2 hits PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 2 hits |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q86B44-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSDANTAVL RNCIRLPLAE DELLEVTAKA KDYAIMHGAA MRSKTAFSPD
60 70 80 90 100
SLNFAPFVLV PSSFPRKEFE KAVALQPIIN RLMHNVAHDE EFITTTLAET
110 120 130 140 150
IKVDEFTANL FNIYRKVLAH GFTQKTSLGM LRSDLMLESG CPELSPRALR
160 170 180 190 200
TAAGEDRGQD AGAAVGQIAG ANGAAGVGTA AGTGSKEEEH REVQLSRVTK
210 220 230 240 250
EPERRATGAQ SAYCCWKQVE INTIASGFGH LGPASKTIQR FVLSELGHAD
260 270 280 290 300
KLHNMPDNNA LAGLCDGMVK AWDIYAKPQA VILFIIEDVS YNICDQRFHE
310 320 330 340 350
FYIRETYPHI KVLRRTLTEV HREGKLGQSK ELLLGSQEVA VIYFRAGYEP
360 370 380 390 400
GHYHSQADWD ARYLMETSLA IKCPSIHYHL AGTKKVQQAL AQPAVLERFI
410 420 430 440 450
NDPEEIKAVG KIFTGLYSLD DNEAGNASYE MALRTPERFV LKPQREGGGN
460 470 480 490 500
NVYGVDIPDA LKRMSRVERS AWILMDLIHP PLTKGYMVRP GGDMPPQIVD
510 520 530 540 550
MVSELGIFGV VIGDAEHIVH NYQAGHMLRT KLSTANEGGV AAGLGALDSP
560
YLIDSDDEDE QK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8IQZ1 | Q8IQZ1_DROME | Glutathione synthetase | Gss2 32495, 6835, CG32495-RB, CG32497, CG33065 | 491 | Annotation score: | ||
Q1RL06 | Q1RL06_DROME | Glutathione synthetase 1, isoform G | Gss2 32495, 6835, CG32497, CG33065, Dmel\CG32495 | 248 | Annotation score: | ||
Q9VX03 | Q9VX03_DROME | Glutathione synthetase 1, isoform F | Gss1 32495, 6835, CG32497, CG33065, Dmel\CG32495 | 134 | Annotation score: | ||
X2JCE7 | X2JCE7_DROME | Glutathione synthetase 1, isoform K | Gss1 32495, 6835, CG32497, CG33065, Dmel\CG32495 | 127 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AAN09450.2 AE014298 Genomic DNA Translation: AAO41699.2 AE014298 Genomic DNA Translation: AAO41700.2 AE014298 Genomic DNA Translation: AAO41701.2 EF419254 mRNA Translation: ABN58449.1 EF419255 mRNA Translation: ABN58450.1 |
RefSeqi | NP_728118.2, NM_167591.2 NP_788925.2, NM_176752.2 NP_788926.2, NM_176753.2 NP_788927.2, NM_176754.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0300972; FBpp0290194; FBgn0030882 FBtr0300973; FBpp0290195; FBgn0030882 FBtr0300975; FBpp0290197; FBgn0052495 FBtr0300979; FBpp0290201; FBgn0052495 |
GeneIDi | 318053 32775 |
UCSCi | CG32495-RC, d. melanogaster CG6835-RC, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AAN09450.2 AE014298 Genomic DNA Translation: AAO41699.2 AE014298 Genomic DNA Translation: AAO41700.2 AE014298 Genomic DNA Translation: AAO41701.2 EF419254 mRNA Translation: ABN58449.1 EF419255 mRNA Translation: ABN58450.1 |
RefSeqi | NP_728118.2, NM_167591.2 NP_788925.2, NM_176752.2 NP_788926.2, NM_176753.2 NP_788927.2, NM_176754.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q86B44, 2 interactors |
STRINGi | 7227.FBpp0290197 |
Genome annotation databases
EnsemblMetazoai | FBtr0300972; FBpp0290194; FBgn0030882 FBtr0300973; FBpp0290195; FBgn0030882 FBtr0300975; FBpp0290197; FBgn0052495 FBtr0300979; FBpp0290201; FBgn0052495 |
GeneIDi | 318053 32775 |
UCSCi | CG32495-RC, d. melanogaster CG6835-RC, d. melanogaster |
Organism-specific databases
CTDi | 318053 32775 |
FlyBasei | FBgn0030882, Gss1 FBgn0052495, Gss2 |
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
GeneTreei | ENSGT00390000013764 |
HOGENOMi | CLU_025152_2_1_1 |
OrthoDBi | 1189955at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
Miscellaneous databases
ChiTaRSi | Galphas, fly |
Gene expression databases
Bgeei | FBgn0030882, Expressed in larva and 18 other tissues |
Family and domain databases
Gene3Di | 1.10.1080.10, 2 hits 3.30.1490.50, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 2 hits PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 2 hits |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q86B44_DROME | |
Accessioni | Q86B44Primary (citable) accession number: Q86B44 Secondary accession number(s): A3FP33, Q8IQZ0 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | June 1, 2003 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 10, 2021 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |