UniProtKB - Q866N2 (MYOC_RABIT)
Myocilin
MYOC
Functioni
Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 366 | CalciumBy similarity | 1 | |
Metal bindingi | 414 | CalciumBy similarity | 1 | |
Metal bindingi | 415 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 463 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 464 | CalciumBy similarity | 1 |
GO - Molecular functioni
- fibronectin binding Source: Ensembl
- frizzled binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- myosin light chain binding Source: Ensembl
- receptor tyrosine kinase binding Source: Ensembl
GO - Biological processi
- bone development Source: UniProtKB
- clustering of voltage-gated sodium channels Source: UniProtKB
- ERBB2-ERBB3 signaling pathway Source: UniProtKB
- myelination in peripheral nervous system Source: UniProtKB
- negative regulation of cell-matrix adhesion Source: UniProtKB
- negative regulation of Rho protein signal transduction Source: UniProtKB
- negative regulation of stress fiber assembly Source: UniProtKB
- neuron projection development Source: UniProtKB
- non-canonical Wnt signaling pathway via JNK cascade Source: UniProtKB
- osteoblast differentiation Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of focal adhesion assembly Source: UniProtKB
- positive regulation of mitochondrial depolarization Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
- positive regulation of protein kinase B signaling Source: UniProtKB
- positive regulation of stress fiber assembly Source: UniProtKB
- positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- regulation of MAPK cascade Source: UniProtKB
- skeletal muscle hypertrophy Source: UniProtKB
- visual perception Source: UniProtKB
Keywordsi
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: MyocilinAlternative name(s): Trabecular meshwork-induced glucocorticoid response protein Cleaved into the following 2 chains: Alternative name(s): Myocilin 20 kDa N-terminal fragment Alternative name(s): Myocilin 35 kDa N-terminal fragment |
Gene namesi | Name:MYOCImported Synonyms:TIGRBy similarity |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Rough endoplasmic reticulum By similarity
Mitochondrion
- Mitochondrion By similarity
- Mitochondrion intermembrane space By similarity
- Mitochondrion inner membrane By similarity
- Mitochondrion outer membrane By similarity
Extracellular region or secreted
- Secreted 1 Publication
- extracellular space By similarity
- extracellular matrix By similarity
- extracellular exosome By similarity
Golgi apparatus
- Golgi apparatus By similarity
Other locations
- Cytoplasmic vesicle By similarity
- Cell projection By similarity
- cilium By similarity
Note: Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum. It is only imported to mitochondria in the trabecular meshwork. Localizes to the Golgi apparatus in Schlemm's canal endothelial cells. Appears in the extracellular space of trabecular meshwork cells by an unconventional mechanism, likely associated with exosome-like vesicles. Localizes in trabecular meshwork extracellular matrix.By similarity
Extracellular region or secreted
- Secreted By similarity
Endoplasmic reticulum
Note: Remains retained in the endoplasmic reticulum.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- rough endoplasmic reticulum Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial intermembrane space Source: UniProtKB
- mitochondrial outer membrane Source: UniProtKB
Other locations
- cilium Source: UniProtKB-SubCell
- collagen-containing extracellular matrix Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB
- node of Ranvier Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000020087 | 19 – 490 | MyocilinSequence analysisAdd BLAST | 472 | |
ChainiPRO_0000428745 | 19 – 212 | Myocilin, N-terminal fragmentBy similarityAdd BLAST | 194 | |
ChainiPRO_0000428746 | 213 – 490 | Myocilin, C-terminal fragmentBy similarityAdd BLAST | 278 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 231 ↔ 419 | PROSITE-ProRule annotationBy similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 212 – 213 | Cleavage; by CAPN2By similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PRIDEi | Q866N2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSOCUG00000015957, Expressed in skeletal muscle tissue and 10 other tissues |
Interactioni
Subunit structurei
Homodimer (via N-terminus). Can also form higher oligomers.
Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC.
Interacts (via N-terminus) with MYL2.
Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling (By similarity).
Interacts with SNTA1; regulates muscle hypertrophy.
Interacts with ERBB2 and ERBB3; activates ERBB2-ERBB3 signaling pathway.
Interacts with SNCG; affects its secretion and its aggregation (By similarity).
By similarityGO - Molecular functioni
- fibronectin binding Source: Ensembl
- frizzled binding Source: Ensembl
- myosin light chain binding Source: Ensembl
- receptor tyrosine kinase binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000013710 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 230 – 489 | Olfactomedin-likePROSITE-ProRule annotationAdd BLAST | 260 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 168 – 187 | DisorderedSequence analysisAdd BLAST | 20 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 55 – 170 | Sequence analysisAdd BLAST | 116 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 488 – 490 | Microbody targeting signalSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 172 – 187 | Polar residuesSequence analysisAdd BLAST | 16 |
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | KOG3545, Eukaryota |
GeneTreei | ENSGT00940000158561 |
HOGENOMi | CLU_035236_4_0_1 |
InParanoidi | Q866N2 |
OMAi | FNMVTYD |
OrthoDBi | 421994at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR031213, Myocilin IPR003112, Olfac-like_dom |
PANTHERi | PTHR23192:SF33, PTHR23192:SF33, 1 hit |
Pfami | View protein in Pfam PF02191, OLF, 1 hit |
SMARTi | View protein in SMART SM00284, OLF, 1 hit |
PROSITEi | View protein in PROSITE PS51132, OLF, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPAVQLLLLA GLVWGAGART AQLRKANDRS GRCQYTFSVA SPSESSCPEQ
60 70 80 90 100
GQTMSAIQDL QRDSSTQRAD LESTKARLSS LESLLHRLTL AQTSGPQEIQ
110 120 130 140 150
EELQKELGTL RRERDQLESQ TRELEAAYSN LLRDKSALEE EKRRLMQENE
160 170 180 190 200
DLARRLESSS QEVARLARGQ CPQARDTSQD VPAGSREASQ WNLDTLAFQE
210 220 230 240 250
LKSELTEVPA SRILKENPPV LPRGEEGDNG CGELVWVGQP VTLRTAETIT
260 270 280 290 300
GKYGVWMRDP KPTSPHTQET TWRIDTVGTD IRQVFEYDRI SQFVQGYPSK
310 320 330 340 350
VYVLPRSLES TGAVVYAGSL YFQGAGSRTV IRFELNTETV KAEKEIPGAG
360 370 380 390 400
YRGQFPYSWG GYTDIDLAVD ETGLWVIYST EEARGAIVLS KLNPENLELE
410 420 430 440 450
KTWETNIRKQ SVANAFIICG TLYTVSSYSS ADATVNFAYD TGTGISKPLA
460 470 480 490
IPFKNRYKYS SMIDYNPLER KLFAWDSFNM VTYDIKLSKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY191317 mRNA Translation: AAO38666.1 |
RefSeqi | NP_001075619.1, NM_001082150.2 |
Genome annotation databases
Ensembli | ENSOCUT00000015953; ENSOCUP00000013710; ENSOCUG00000015957 |
GeneIDi | 100008897 |
KEGGi | ocu:100008897 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY191317 mRNA Translation: AAO38666.1 |
RefSeqi | NP_001075619.1, NM_001082150.2 |
3D structure databases
AlphaFoldDBi | Q866N2 |
SMRi | Q866N2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000013710 |
Proteomic databases
PRIDEi | Q866N2 |
Genome annotation databases
Ensembli | ENSOCUT00000015953; ENSOCUP00000013710; ENSOCUG00000015957 |
GeneIDi | 100008897 |
KEGGi | ocu:100008897 |
Organism-specific databases
CTDi | 4653 |
Phylogenomic databases
eggNOGi | KOG3545, Eukaryota |
GeneTreei | ENSGT00940000158561 |
HOGENOMi | CLU_035236_4_0_1 |
InParanoidi | Q866N2 |
OMAi | FNMVTYD |
OrthoDBi | 421994at2759 |
Gene expression databases
Bgeei | ENSOCUG00000015957, Expressed in skeletal muscle tissue and 10 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR031213, Myocilin IPR003112, Olfac-like_dom |
PANTHERi | PTHR23192:SF33, PTHR23192:SF33, 1 hit |
Pfami | View protein in Pfam PF02191, OLF, 1 hit |
SMARTi | View protein in SMART SM00284, OLF, 1 hit |
PROSITEi | View protein in PROSITE PS51132, OLF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MYOC_RABIT | |
Accessioni | Q866N2Primary (citable) accession number: Q866N2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |