UniProtKB - Q84ZW8 (ACSS_MAIZE)
Protein
Acyclic sesquiterpene synthase
Gene
TPS1
Organism
Zea mays (Maize)
Status
Functioni
Involved in the formation of (E)-beta-farnesene and (3E)-4,8-dimethyl-1,3,7-nonatriene, key signal molecules in induced plant defense mediated by the attraction of enemies of herbivores (PubMed:12481088). In the presence of geranyl diphosphate, catalyzes the formation of the acyclic monoterpens (3R)-linalool and geraniol (PubMed:12481088). However, the in vitro rate of sesquiterpene formation is about four times higher than the rate of monoterpene formation (PubMed:12481088). Mediates the conversion of ent-copalyl diphosphate into ent-kaurene, thus having probably a role in gibberellin biosynthesis (PubMed:26620527).2 Publications
Catalytic activityi
- EC:4.2.3.471 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.492 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:3.1.7.61 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.1.7.111 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.261 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.3.191 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Kineticsi
- KM=1.1 µM for geranyl diphosphate1 Publication
- KM=1.0 µM for farnesyl diphosphate1 Publication
- KM=470 µM for magnesium1 Publication
- KM=26 µM for manganese1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
Temperature dependencei
Optimum temperature is between 30 and 37 degrees Celsius.1 Publication
: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 333 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 333 | Magnesium or manganese 2By similarity | 1 | |
Binding sitei | 333 | SubstrateBy similarity | 1 | |
Metal bindingi | 337 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 337 | Magnesium or manganese 2By similarity | 1 | |
Binding sitei | 337 | SubstrateBy similarity | 1 | |
Binding sitei | 474 | SubstrateBy similarity | 1 | |
Metal bindingi | 477 | Magnesium or manganese 3By similarity | 1 | |
Binding sitei | 477 | SubstrateBy similarity | 1 | |
Metal bindingi | 481 | Magnesium or manganese 3By similarity | 1 | |
Metal bindingi | 485 | Magnesium or manganese 3By similarity | 1 |
GO - Molecular functioni
- (3R)-(E)-nerolidol synthase activity Source: UniProtKB
- ent-kaurene synthase activity Source: UniProtKB
- hydrolase activity Source: UniProtKB-KW
- magnesium ion binding Source: GO_Central
- R-linalool synthase activity Source: UniProtKB
- terpene synthase activity Source: UniProtKB
GO - Biological processi
- defense response to fungus Source: UniProtKB
- diterpenoid biosynthetic process Source: UniProtKB
- ent-kaurene biosynthetic process Source: UniProtKB
- farnesol biosynthetic process Source: UniProtKB
- geraniol biosynthetic process Source: UniProtKB
- response to ethylene Source: UniProtKB
- response to jasmonic acid Source: UniProtKB
- sesquiterpenoid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Lyase |
Biological process | Plant defense |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-12827 |
BRENDAi | 4.2.3.49, 6752 |
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyclic sesquiterpene synthaseAlternative name(s): (3R,6E)-nerolidol synthase1 Publication (EC:4.2.3.492 Publications) (R)-linalool synthase1 Publication (EC:4.2.3.261 Publication) Beta-farnesene synthase1 Publication (EC:4.2.3.471 Publication) Farnesyl diphosphatase1 Publication (EC:3.1.7.61 Publication) Geraniol synthase1 Publication (EC:3.1.7.111 Publication) Terpene synthase 11 Publication Short name: ZmTPS11 Publication |
Gene namesi | Name:TPS11 Publication Synonyms:csu186Curated, eks1Curated, KS2.11 Publication ORF Names:GRMZM2G049538Curated, ZEAMMB73_Zm00001d002351Imported |
Organismi | Zea mays (Maize) |
Taxonomic identifieri | 4577 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Tripsacinae › Zea |
Proteomesi |
|
Organism-specific databases
MaizeGDBi | 1219908 |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Other locations
- Cytoplasm By similarity
Chloroplast
- chloroplast Source: UniProtKB
Keywords - Cellular componenti
Chloroplast, Cytoplasm, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 53 | ChloroplastSequence analysisAdd BLAST | 53 | |
ChainiPRO_0000405117 | 54 – 590 | Acyclic sesquiterpene synthaseAdd BLAST | 537 |
Proteomic databases
PaxDbi | Q84ZW8 |
Expressioni
Tissue specificityi
Expressed in leaves and sheath tissues. Not detected in roots.1 Publication
Inductioni
By herbivory, wounding or elicitor treatments in young leaves of cv. B73, but not of cv. Delprim (PubMed:12481088). Induced transiently by F.graminearum and combination of jasmonic acid (JA) and ethephon (EP), an ethylene precursor (PubMed:26620527).2 Publications
Gene expression databases
ExpressionAtlasi | Q84ZW8, baseline and differential |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 4577.GRMZM2G049538_P01 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 333 – 337 | DDXXD motifBy similarity | 5 |
Domaini
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity
Sequence similaritiesi
Belongs to the terpene synthase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502QVGX, Eukaryota |
OrthoDBi | 318477at2759 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q84ZW8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMPVKLTPA SLSLKAVCCR FSSGGHALRF GSSLPCWRRT PTQRSTSSST
60 70 80 90 100
TRPAAEVSSG KSKQHDQEAS EATIRQQLQL VDVLENMGIS RHFAAEIKCI
110 120 130 140 150
LDRTYRSWLQ RHEEIMLDTM TCAMAFRILR LNGYNVSSDE LYHVVEASGL
160 170 180 190 200
HNSLGGYLND TRTLLELHKA STVSISEDES ILDSIGSRSR TLLREQLESG
210 220 230 240 250
GALRKPSLFK EVEHALDGPF YTTLDRLHHR WNIENFNIIE QHMLETPYLS
260 270 280 290 300
NQHTSRDILA LSIRDFSSSQ FTYQQELQHL ESWVKECRLD QLQFARQKLA
310 320 330 340 350
YFYLSAAGTM FSPELSDART LWAKNGVLTT IVDDFFDVAG SKEELENLVM
360 370 380 390 400
LVEMWDEHHK VEFYSEQVEI IFSSIYDSVN QLGEKASLVQ DRSITKHLVE
410 420 430 440 450
IWLDLLKSMM TEVEWRLSKY VPTEKEYMIN ASLTFGLGPI VLPALYFVGP
460 470 480 490 500
KISESIVKDP EYDELFKLMS TCGRLLNDVQ TFEREYNEGK LNSVSLLVLH
510 520 530 540 550
GGSMSISDAK RKLQKPIDTC RRDLLSLVLR EESVVPRPCK ELFWKMCKVC
560 570 580 590
YFFYSTTDGF SSQVERAKEV DAVINEPLKL QGSHTLVSDV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1D6DZW3 | A0A1D6DZW3_MAIZE | Terpene synthase1 | 541974 ZEAMMB73_Zm00001d002351 | 538 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 434 | T → I in AAO18435 (PubMed:12481088). | 1 | |
Sequence conflicti | 503 | S → P in AAO18435 (PubMed:12481088). | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF529266 mRNA Translation: AAO18435.1 CM007648 Genomic DNA Translation: ONM14044.1 AF105149 mRNA Translation: AAD34319.1 |
RefSeqi | NP_001105097.2, NM_001111627.2 |
Genome annotation databases
EnsemblPlantsi | Zm00001d002351_T001; Zm00001d002351_P001; Zm00001d002351 |
GeneIDi | 541974 |
Gramenei | Zm00001d002351_T001; Zm00001d002351_P001; Zm00001d002351 |
KEGGi | zma:541974 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF529266 mRNA Translation: AAO18435.1 CM007648 Genomic DNA Translation: ONM14044.1 AF105149 mRNA Translation: AAD34319.1 |
RefSeqi | NP_001105097.2, NM_001111627.2 |
3D structure databases
SMRi | Q84ZW8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4577.GRMZM2G049538_P01 |
Proteomic databases
PaxDbi | Q84ZW8 |
Genome annotation databases
EnsemblPlantsi | Zm00001d002351_T001; Zm00001d002351_P001; Zm00001d002351 |
GeneIDi | 541974 |
Gramenei | Zm00001d002351_T001; Zm00001d002351_P001; Zm00001d002351 |
KEGGi | zma:541974 |
Organism-specific databases
MaizeGDBi | 1219908 |
Phylogenomic databases
eggNOGi | ENOG502QVGX, Eukaryota |
OrthoDBi | 318477at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
BioCyci | MetaCyc:MONOMER-12827 |
BRENDAi | 4.2.3.49, 6752 |
Gene expression databases
ExpressionAtlasi | Q84ZW8, baseline and differential |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACSS_MAIZE | |
Accessioni | Q84ZW8Primary (citable) accession number: Q84ZW8 Secondary accession number(s): A0A1D6DZW2, Q9XEI5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 8, 2011 |
Last sequence update: | July 3, 2019 | |
Last modified: | February 10, 2021 | |
This is version 83 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families