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Protein

Type II inositol polyphosphate 5-phosphatase 15

Gene

IP5P15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Has a higher substrate affinity toward PtdIns(4,5)P2. Required for secondary wall synthesis and actin organization in fiber cells.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.1 Publication
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3,4-diphosphate + phosphate.1 Publication
D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=63 µM for PtdIns(4,5)P2 (at pH 7.0 and 22 degrees Celsius)
  2. KM=299 µM for PtdIns(3,4,5)P3 (at pH 7.0 and 22 degrees Celsius)
  3. KM=1040 µM for PtdIns(1,4,5)P3 (at pH 6.5 and 22 degrees Celsius)
  1. Vmax=240 pmol/min/µg enzyme with PtdIns(4,5)P2 as substrate (at pH 7.0 and 22 degrees Celsius)
  2. Vmax=365 pmol/min/µg enzyme with PtdIns(3,4,5)P3 as substrate (at pH 7.0 and 22 degrees Celsius)
  3. Vmax=504 pmol/min/µg enzyme with Ins(1,4,5)P3 as substrate (at pH 6.5 and 22 degrees Celsius)

pH dependencei

Optimum pH is 6.5 with Ins(1,4,5)P3 as substrate, 7.5 with PtdIns(3,4,5)P3 as substrate, and 6.5-8.5 with PtdIns(4,5)P2 as substrate.

Temperature dependencei

Optimum temperature is 22 degrees Celsius with Ins(1,4,5)P3 as substrate, and 22-37 degrees Celsius with PtdIns(4,5)P2 and PtdIns(3,4,5)P3 as substrates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G65580-MONOMER
BRENDAi3.1.3.36 399
ReactomeiR-ATH-1660499 Synthesis of PIPs at the plasma membrane
R-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Type II inositol polyphosphate 5-phosphatase 15Curated (EC:3.1.3.361 Publication, EC:3.1.3.861 Publication)
Short name:
At5PTase15Curated
Alternative name(s):
Protein FRAGILE FIBER 31 Publication
Gene namesi
Name:IP5P15Curated
Synonyms:FRA31 Publication
Ordered Locus Names:At1g65580Imported
ORF Names:F5I14.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G65580
TAIRilocus:2034141 AT1G65580

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi833A → V in fra3; induces a dramatic reduction in secondary wall thickness and a concomitant decrease in stem strength. Causes elevated levels of PtdIns(4,5)P2 and Ins(1,4,5)P3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097261 – 1101Type II inositol polyphosphate 5-phosphatase 15Add BLAST1101

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki907Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ84W55
PRIDEiQ84W55

PTM databases

iPTMnetiQ84W55

Expressioni

Tissue specificityi

Predominantly expressed in interfascicular fibers and vascular bundles. Expressed in seedlings, stems, roots and flowers. Expressed at lower level in mature leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ84W55 baseline and differential
GenevisibleiQ84W55 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G65580.1

Structurei

3D structure databases

ProteinModelPortaliQ84W55
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati121 – 162WD 1Add BLAST42
Repeati180 – 219WD 2Add BLAST40
Repeati225 – 263WD 3Add BLAST39
Repeati403 – 432WD 4Add BLAST30
Repeati433 – 481WD 5Add BLAST49
Repeati483 – 519WD 6Add BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni749 – 765Catalytic 1By similarityAdd BLAST17
Regioni828 – 843Catalytic 2By similarityAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 52Ser-richAdd BLAST39

Sequence similaritiesi

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0565 Eukaryota
COG5411 LUCA
HOGENOMiHOG000242921
InParanoidiQ84W55
OMAiWLDMIGK
OrthoDBiEOG093600RQ
PhylomeDBiQ84W55

Family and domain databases

Gene3Di2.130.10.10, 2 hits
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000300 IPPc
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SM00320 WD40, 5 hits
SUPFAMiSSF56219 SSF56219, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q84W55-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEDRQNDQND DVFSFFSPSF SAATPSTLFN RSAYSSSSSS GDDESQPSVD
60 70 80 90 100
DSNKRIDYMI QFLDRRLSED GNHDGIGDGN GSDSLPEFVG KCGESGIFKV
110 120 130 140 150
PIRSAVHPNR PPSLDVRPHP LRETQIGRFL RTMTSTERQL WTGGEDGALR
160 170 180 190 200
VWEFSELYGS GRGLEVEDTA PYKESLGNEF GSAAVVCMIG DEGSRVVWSG
210 220 230 240 250
HRDGRIRCWR LRGDHGIEEA LSWQAHRGPV LSIAISAYGD IWSGSEGGAL
260 270 280 290 300
KVWPWDGALG KSLSLKMEER HMAALAVERS YIDPRNMVSA NGFANTLTSD
310 320 330 340 350
VTFLVSDHTR ARVWSASPLT FAIWDARTRD LIKVFNIDGQ LENRPENSVY
360 370 380 390 400
PDFGSEEEGK MKVTASKKEK AQSSLGFFQR SRNAIMGAAD AVRRAATKGG
410 420 430 440 450
FCDDSRKTEA IVISVDGMIW TGSSNGILMR WDGNGNCLQE FAYESSGILC
460 470 480 490 500
MFTFCSRLWV GYSNGTVQVW DLEGKLLGGW VAHSGPVIKM AIGAGYLFTL
510 520 530 540 550
ANHGGIRGWN VTSPGPLDNV LRAELAGKEF LYSRIENLKI LAGTWNVGEG
560 570 580 590 600
RASTDSLVSW LGCAATGVEI VVVGLQEVEM GAGVLAMSAA KETVGLEGSP
610 620 630 640 650
LGQWWLDMIG KTLDEGSSFV RVGSRQLAGL LICVWVRHDL KPHVGDVDAA
660 670 680 690 700
AVPCGFGRAI GNKGAVGVRL RMYDRVLCFV NCHFAAHLEA VNRRNADFDH
710 720 730 740 750
VYRTMTFSRQ SSSLNAGVAG ASFGVTMPRG GNALGVNTIE ARPELSEADM
760 770 780 790 800
VIFLGDFNYR LDDITYDETR DFISQRCFDW LREKDQLHTE MEAGNVFQGM
810 820 830 840 850
REAIIRFPPT YKFERHQAGL AGYDSGEKKR IPAWCDRILY RDNKKHLGAE
860 870 880 890 900
CSLDCPVVSS ISQYDACMEV TDSDHKPVRC VFSVKIARVD ESVRRQEYGN
910 920 930 940 950
IINSNKKIKV LLGELSKVPE TIVSTNNIIL QNQDSTILRI TNKSEKNIAF
960 970 980 990 1000
FKIICEGQSK IEEDGQAHDH RARGSFGFPQ WLEVSPGTGT IKPNQIAEVS
1010 1020 1030 1040 1050
VHLEDFPTVE EFVDGVAQNS WCEDTRDKEV ILVLVVHGRF STETRKHRIR
1060 1070 1080 1090 1100
VRHCPRGGPA KNHFNDGTKT SGQINALHRS DYHQLSNTLD VVEQLKNLHS

P
Length:1,101
Mass (Da):121,711
Last modified:February 1, 2005 - v2
Checksum:i256882EB27511965
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1P8AR99A0A1P8AR99_ARATH
Endonuclease/exonuclease/phosphatas...
FRA3 FRAGILE FIBER3, At1g65580, F5I14.11, F5I14_11
835Annotation score:

Sequence cautioni

The sequence AAB60921 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti583G → R in AAO42229 (PubMed:14593172).Curated1
Sequence conflicti847L → F in CAB41466 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY761186 mRNA Translation: AAV87313.1
AY761190 Genomic DNA Translation: AAV87317.1
AJ005682 mRNA Translation: CAB41466.1
AC001229 Genomic DNA Translation: AAB60921.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34398.1
BT004211 mRNA Translation: AAO42229.1
PIRiG96680
RefSeqiNP_176736.2, NM_105232.3
UniGeneiAt.414

Genome annotation databases

EnsemblPlantsiAT1G65580.1; AT1G65580.1; AT1G65580
GeneIDi842869
GrameneiAT1G65580.1; AT1G65580.1; AT1G65580
KEGGiath:AT1G65580

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY761186 mRNA Translation: AAV87313.1
AY761190 Genomic DNA Translation: AAV87317.1
AJ005682 mRNA Translation: CAB41466.1
AC001229 Genomic DNA Translation: AAB60921.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34398.1
BT004211 mRNA Translation: AAO42229.1
PIRiG96680
RefSeqiNP_176736.2, NM_105232.3
UniGeneiAt.414

3D structure databases

ProteinModelPortaliQ84W55
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G65580.1

PTM databases

iPTMnetiQ84W55

Proteomic databases

PaxDbiQ84W55
PRIDEiQ84W55

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G65580.1; AT1G65580.1; AT1G65580
GeneIDi842869
GrameneiAT1G65580.1; AT1G65580.1; AT1G65580
KEGGiath:AT1G65580

Organism-specific databases

AraportiAT1G65580
TAIRilocus:2034141 AT1G65580

Phylogenomic databases

eggNOGiKOG0565 Eukaryota
COG5411 LUCA
HOGENOMiHOG000242921
InParanoidiQ84W55
OMAiWLDMIGK
OrthoDBiEOG093600RQ
PhylomeDBiQ84W55

Enzyme and pathway databases

BioCyciARA:AT1G65580-MONOMER
BRENDAi3.1.3.36 399
ReactomeiR-ATH-1660499 Synthesis of PIPs at the plasma membrane
R-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol

Miscellaneous databases

PROiPR:Q84W55

Gene expression databases

ExpressionAtlasiQ84W55 baseline and differential
GenevisibleiQ84W55 AT

Family and domain databases

Gene3Di2.130.10.10, 2 hits
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000300 IPPc
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SM00320 WD40, 5 hits
SUPFAMiSSF56219 SSF56219, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIP5PF_ARATH
AccessioniPrimary (citable) accession number: Q84W55
Secondary accession number(s): O04475, Q5MK18, Q9XFT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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