UniProtKB - Q84VZ1 (FHY1C_ARATH)
Flavin mononucleotide hydrolase 1, chloroplatic
FHY1
Functioni
FMN hydrolase that catalyzes the dephosphorylation of flavin mononucleotide (FMN) to riboflavin (PubMed:22002057, PubMed:24123841, PubMed:27490826).
Can also dephosphorylate 5-amino-6-(5-phospho-D-ribitylamino)uracil, also known as ARPP (PubMed:24123841, PubMed:27490826).
Not required for riboflavin biosynthesis in planta, but may help maintaining flavin homeostasis within chloroplasts (PubMed:27490826).
3 PublicationsCatalytic activityi
- EC:3.1.3.1021 Publication
- 5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O = 5-amino-6-(D-ribitylamino)uracil + phosphate2 PublicationsEC:3.1.3.1042 Publications
Cofactori
Kineticsi
- KM=14.2 µM for FMN1 Publication
- KM=128 µM for 5-amino-6-(5-phospho-D-ribitylamino)uracil1 Publication
- Vmax=1.42 µmol/min/mg enzyme with FMN as substrate1 Publication
- Vmax=6.73 µmol/min/mg enzyme with 5-amino-6-(5-phospho-D-ribitylamino)uracil as substrate1 Publication
pH dependencei
Temperature dependencei
GO - Molecular functioni
- 5-amino-6-(5-phosphoribitylamino)uracil phosphatase activity Source: RHEA
- FMN hydrolase activity Source: TAIR
- protein self-association Source: TAIR
GO - Biological processi
- riboflavin biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Riboflavin biosynthesis |
Enzyme and pathway databases
BRENDAi | 3.1.3.104, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Flavin mononucleotide hydrolase 1, chloroplatic1 Publication (EC:3.1.3.1021 Publication)Short name: AtcpFHy11 Publication Alternative name(s): 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase1 Publication (EC:3.1.3.1042 Publications) Short name: AtcpFHy1/PyrP11 Publication 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate phosphatase1 Publication Short name: ARPP phosphatase1 Publication |
Gene namesi | Name:FHY11 Publication Synonyms:PYRP11 Publication Ordered Locus Names:At1g79790Imported ORF Names:F20B17.21Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress)Imported |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G79790 |
TAIRi | locus:2019873, AT1G79790 |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | ChloroplastSequence analysisAdd BLAST | 26 | |
ChainiPRO_0000439662 | 27 – 245 | Flavin mononucleotide hydrolase 1, chloroplaticSequence analysisAdd BLAST | 219 |
Proteomic databases
PaxDbi | Q84VZ1 |
PRIDEi | Q84VZ1 |
ProteomicsDBi | 230717 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein self-association Source: TAIR
Protein-protein interaction databases
STRINGi | 3702.AT1G79790.1 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3085, Eukaryota |
HOGENOMi | CLU_080554_2_0_1 |
InParanoidi | Q84VZ1 |
OMAi | PPCMAST |
PhylomeDBi | Q84VZ1 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAAAAMHTSA EFINLKPNMW KKNPVRASGS CCLGSSSGDE ISRKRKLPIL
60 70 80 90 100
LFDVMDTIVR DPFYQDVPAF FGMPMKQLLE CKHPMVWIEF EKGLIDEEEL
110 120 130 140 150
ARNFFIDGRD FDLEGLKECM RSGYSYLDGM QELLQTLAAD DFEIHAFTNY
160 170 180 190 200
PIWYNIIEDK LKLSAYLSWT FCSCIAGKRK PDPEFYLEVV GHLGVEPCDC
210 220 230 240
IFIDDRPTNV KCAIEIGMGG LCFENADSLA KDLSDLGINV SVPKL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4HQA8 | F4HQA8_ARATH | Haloacid dehalogenase-like hydrolas... | FHY1 AtcpFHy1, At1g79790, F20B17.21, F20B17_21 | 270 | Annotation score: | ||
A0A1P8AWF1 | A0A1P8AWF1_ARATH | Haloacid dehalogenase-like hydrolas... | FHY1 AtcpFHy1, At1g79790, F20B17.21, F20B17_21 | 151 | Annotation score: | ||
A0A1P8AWB4 | A0A1P8AWB4_ARATH | Haloacid dehalogenase-like hydrolas... | FHY1 AtcpFHy1, At1g79790, F20B17.21, F20B17_21 | 31 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | Missing in AAM63745 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC010793 Genomic DNA Translation: AAF68115.1 Sequence problems. CP002684 Genomic DNA Translation: AEE36298.1 BT004602 mRNA Translation: AAO42848.1 AK227909 mRNA Translation: BAE99879.1 AY086690 mRNA Translation: AAM63745.1 |
RefSeqi | NP_565221.1, NM_106628.3 |
Genome annotation databases
EnsemblPlantsi | AT1G79790.1; AT1G79790.1; AT1G79790 |
GeneIDi | 844318 |
Gramenei | AT1G79790.1; AT1G79790.1; AT1G79790 |
KEGGi | ath:AT1G79790 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC010793 Genomic DNA Translation: AAF68115.1 Sequence problems. CP002684 Genomic DNA Translation: AEE36298.1 BT004602 mRNA Translation: AAO42848.1 AK227909 mRNA Translation: BAE99879.1 AY086690 mRNA Translation: AAM63745.1 |
RefSeqi | NP_565221.1, NM_106628.3 |
3D structure databases
SMRi | Q84VZ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G79790.1 |
Proteomic databases
PaxDbi | Q84VZ1 |
PRIDEi | Q84VZ1 |
ProteomicsDBi | 230717 |
Genome annotation databases
EnsemblPlantsi | AT1G79790.1; AT1G79790.1; AT1G79790 |
GeneIDi | 844318 |
Gramenei | AT1G79790.1; AT1G79790.1; AT1G79790 |
KEGGi | ath:AT1G79790 |
Organism-specific databases
Araporti | AT1G79790 |
TAIRi | locus:2019873, AT1G79790 |
Phylogenomic databases
eggNOGi | KOG3085, Eukaryota |
HOGENOMi | CLU_080554_2_0_1 |
InParanoidi | Q84VZ1 |
OMAi | PPCMAST |
PhylomeDBi | Q84VZ1 |
Enzyme and pathway databases
BRENDAi | 3.1.3.104, 399 |
Miscellaneous databases
PROi | PR:Q84VZ1 |
Gene expression databases
ExpressionAtlasi | Q84VZ1, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FHY1C_ARATH | |
Accessioni | Q84VZ1Primary (citable) accession number: Q84VZ1 Secondary accession number(s): Q8LCB6, Q9MA00 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2017 |
Last sequence update: | June 1, 2003 | |
Last modified: | September 29, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families