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Protein

Leucine aminopeptidase

Gene

LKHA4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA4 but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B4 as the product compared to the homologous mammalian enzyme (in vitro).By similarity

Catalytic activityi

An epoxide + H2O = a glycol.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi305Zinc; catalyticPROSITE-ProRule annotation1
Active sitei306Proton acceptorPROSITE-ProRule annotation1
Metal bindingi309Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi328Zinc; catalyticPROSITE-ProRule annotation1
Active sitei393Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processLeukotriene biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-OSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-OSA-6798695 Neutrophil degranulation
R-OSA-9018676 Biosynthesis of D-series resolvins
R-OSA-9018681 Biosynthesis of protectins
R-OSA-9018896 Biosynthesis of E-series 18(S)-resolvins
R-OSA-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-OSA-9023661 Biosynthesis of E-series 18(R)-resolvins

Protein family/group databases

MEROPSiM01.A26

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine aminopeptidase (EC:3.4.11.-)
Alternative name(s):
Epoxide hydrolase (EC:3.3.2.10)
Leukotriene A-4 hydrolase homolog
Short name:
LTA-4 hydrolase
Gene namesi
Name:LKHA4
Ordered Locus Names:Os03g0819100, LOC_Os03g60460
ORF Names:OJ1754_E06.9, OsJ_13129
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004245891 – 611Leucine aminopeptidaseAdd BLAST611

Proteomic databases

PaxDbiQ84TA3
PRIDEiQ84TA3

PTM databases

iPTMnetiQ84TA3

Expressioni

Gene expression databases

ExpressionAtlasiQ84TA3 differential
GenevisibleiQ84TA3 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g60460.1

Structurei

3D structure databases

ProteinModelPortaliQ84TA3
SMRiQ84TA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131Substrate bindingBy similarity3
Regioni278 – 282Substrate bindingBy similarity5
Regioni562 – 564Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047 Eukaryota
COG0308 LUCA
InParanoidiQ84TA3
KOiK01254
OMAiNFEHFWL
OrthoDBiEOG093606IA

Family and domain databases

CDDicd09599 M1_LTA4H, 1 hit
Gene3Di1.10.390.10, 1 hit
1.25.40.320, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR038502 M1_LTA-4_hydro/amino_C_sf
IPR034015 M1_LTA4H
IPR001930 Peptidase_M1
IPR015211 Peptidase_M1_C
IPR014782 Peptidase_M1_N
IPR027268 Peptidase_M4/M1_CTD_sf
PANTHERiPTHR11533 PTHR11533, 1 hit
PfamiView protein in Pfam
PF09127 Leuk-A4-hydro_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
SMARTiView protein in SMART
SM01263 Leuk-A4-hydro_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q84TA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVDPHSYT DGDHPVTAKA ALAFYLDFAA STIHASALLT LSAPHSGDLL
60 70 80 90 100
LDTRALAVHS ASTASGPPSP IPFSLADAAD PVLGSALTLT LPPDTTSFLL
110 120 130 140 150
TFSTSPSASA LQWLSPPQTA SSLPFVFSQC QSIHARSVFP CHDTPAARIT
160 170 180 190 200
FDLLLNVPTQ LSAVAAARHV SRRDPLPSDH RGACDDALWC APGRIVEEFQ
210 220 230 240 250
MEQSVPPYLF AFAAGGIGFR DLGPRTRVYA EGGDKVLDEA AREFAGVEEM
260 270 280 290 300
VKVGESLFGP YEWERFDLLV LPPSFPYGGM ENPRMVFLTP TVIKGDAAGA
310 320 330 340 350
QVVAHELAHS WTGNLITNKT NEDFWLNEGF TTYAERRIVE VVQGEERAAL
360 370 380 390 400
NMGIGWRGLN RMMERFKDNM EYTKLKPKMA GIDPDDVYSE VPYEKGFQFL
410 420 430 440 450
WRIERQIGRP AFDEFLKNYI STFKFKSIDT ETFLEFLKTN VPGIENQIDL
460 470 480 490 500
QLWIEGTGIP PDAMEPESAI YKKICSLAAE FKSGKLPSED EVADWSGQEW
510 520 530 540 550
ELYLENLPTD VEASQVTALD ERYKLSESCD YEVKVAFLQL AIPTGCRCYF
560 570 580 590 600
NEVEKCLKQV GRMKYLRPLY SSLARCSGEE KMLAHRIFSE AHEFYHPIAR
610
SVAESILSKH G
Length:611
Mass (Da):67,792
Last modified:June 1, 2003 - v1
Checksum:i9A99FA6F56C4D8FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → P in AK073744 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC104433 Genomic DNA Translation: AAO65874.1
DP000009 Genomic DNA Translation: ABF99571.1
AP008209 Genomic DNA Translation: BAF13624.1
AP014959 Genomic DNA Translation: BAS87073.1
CM000140 Genomic DNA Translation: EAZ29074.1
AK073744 mRNA No translation available.
RefSeqiXP_015632326.1, XM_015776840.1
UniGeneiOs.15432

Genome annotation databases

EnsemblPlantsiOs03t0819100-01; Os03t0819100-01; Os03g0819100
GeneIDi4334583
GrameneiOs03t0819100-01; Os03t0819100-01; Os03g0819100
KEGGiosa:4334583

Similar proteinsi

Entry informationi

Entry nameiLKHA4_ORYSJ
AccessioniPrimary (citable) accession number: Q84TA3
Secondary accession number(s): A0A0N7KIA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: June 1, 2003
Last modified: June 20, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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