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UniProtKB - Q84P23 (4CLL9_ARATH)
Protein
4-coumarate--CoA ligase-like 9
Gene
4CLL9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Contributes to jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of its precursors (PubMed:15677481, PubMed:18267944).
Converts 12-oxo-phytodienoic acid (OPDA) into OPDA-CoA (PubMed:15677481, PubMed:18267944).
2 PublicationsCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z,12Z,15Z)-octadecatrienoate + ATP + CoA = (9Z,12Z,15Z)-octadecatrienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (10Z,15Z)-12-oxophytodienoate + ATP + CoA = (10Z,15Z)-12-oxophytodienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
kcat is 2 sec(-1) with tetradecanoic acid as substrate (PubMed:15677481, PubMed:18267944). kcat is 2.62 sec(-1) with OPDA as substrate (PubMed:15677481, PubMed:18267944). kcat is 19.87 sec(-1) with nonanoic acid as substrate (PubMed:15677481, PubMed:18267944). kcat is 2.79 sec(-1) with hexanoic acid as substrate (PubMed:15677481, PubMed:18267944).2 Publications
- KM=6302 µM for hexanoic acid2 Publications
- KM=2125 µM for nonanoic acid2 Publications
- KM=11 µM for OPDA2 Publications
- KM=35 µM for hexanoic acid2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 259 | ATPBy similarity | 1 | |
Binding sitei | 357 | ATPBy similarity | 1 | |
Binding sitei | 438 | ATPBy similarity | 1 | |
Binding sitei | 453 | ATPBy similarity | 1 | |
Binding sitei | 544 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 212 – 216 | ATPBy similarity | 5 | |
Nucleotide bindingi | 330 – 332 | ATPBy similarity | 3 | |
Nucleotide bindingi | 352 – 353 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CoA-ligase activity Source: GO_Central
- decanoate-CoA ligase activity Source: RHEA
- fatty-acyl-CoA synthase activity Source: TAIR
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
- jasmonic acid biosynthetic process Source: TAIR
- oxylipin biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT5G63380-MONOMER |
SABIO-RKi | Q84P23 |
Chemistry databases
SwissLipidsi | SLP:000001792 |
Names & Taxonomyi
Protein namesi | Recommended name: 4-coumarate--CoA ligase-like 91 Publication (EC:6.2.1.-2 Publications)Alternative name(s): 4-coumarate--CoA ligase isoform 4 Short name: At4CL4 |
Gene namesi | Name:4CLL91 Publication Ordered Locus Names:At5g63380Imported ORF Names:K9H21.11Imported, K9H21.8Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G63380 |
TAIRi | locus:2158559, AT5G63380 |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Peroxisome
- peroxisome Source: TAIR
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Disruption phenotypei
No obvious phenotype in growth, root and flower development, fertility, reproduction and morphology.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000299182 | 1 – 562 | 4-coumarate--CoA ligase-like 9Add BLAST | 562 |
Proteomic databases
PaxDbi | Q84P23 |
PRIDEi | Q84P23 |
ProteomicsDBi | 245107 |
PTM databases
iPTMneti | Q84P23 |
Expressioni
Tissue specificityi
Expressed at low level in leaves.1 Publication
Gene expression databases
ExpressionAtlasi | Q84P23, baseline and differential |
Genevisiblei | Q84P23, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 283 – 352 | SBD1By similarityAdd BLAST | 70 | |
Regioni | 353 – 417 | SBD2By similarityAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 560 – 562 | Microbody targeting signalSequence analysis | 3 |
Domaini
Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.By similarity
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
HOGENOMi | CLU_000022_59_2_1 |
InParanoidi | Q84P23 |
OMAi | EYRIICT |
OrthoDBi | 683933at2759 |
PhylomeDBi | Q84P23 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q84P23-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTKTNDSRL IDRSSGFDQR TGIYHSLRPS LSLPPIDQPL SAAEFALSLL
60 70 80 90 100
LKSSPPATAG KNIEALTYLV NSSSGDNLTY GELLRRVRSL AVSLRERFPS
110 120 130 140 150
LASRNVAFIL SPSSLDIPVL YLALMSIGVV VSPANPIGSE SEVSHQVEVS
160 170 180 190 200
EPVIAFATSQ TVKKLQSSSL PLGTVLMDST EFLSWLNRSD SSSVNPFQVQ
210 220 230 240 250
VNQSDPAAIL FSSGTTGRVK GVLLTHRNLI ASTAVSHQRT LQDPVNYDRV
260 270 280 290 300
GLFSLPLFHV FGFMMMIRAI SLGETLVLLG RFELEAMFKA VEKYKVTGMP
310 320 330 340 350
VSPPLIVALV KSELTKKYDL RSLRSLGCGG APLGKDIAER FKQKFPDVDI
360 370 380 390 400
VQGYGLTESS GPAASTFGPE EMVKYGSVGR ISENMEAKIV DPSTGESLPP
410 420 430 440 450
GKTGELWLRG PVIMKGYVGN EKASAETVDK EGWLKTGDLC YFDSEDFLYI
460 470 480 490 500
VDRLKELIKY KAYQVPPVEL EQILHSNPDV IDAAVVPFPD EDAGEIPMAF
510 520 530 540 550
IVRKPGSNLN EAQIIDFVAK QVTPYKKVRR VAFINAIPKN PAGKILRREL
560
TKIAVDGNAS KL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ9FGW7 | Q9FGW7_ARATH | Emb|CAB62340.1 | At5g63350, K9H21.8, K9H21_8 | 302 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 523 | T → A in AAP03018 (PubMed:12805634).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY250835 mRNA Translation: AAP03018.1 AY376731 mRNA Translation: AAQ86590.1 AB023035, AB007649 Genomic DNA Translation: BAB10742.1 CP002688 Genomic DNA Translation: AED97741.1 AY136459 mRNA Translation: AAM97124.1 BT010394 mRNA Translation: AAQ56837.1 |
RefSeqi | NP_201143.1, NM_125733.3 |
Genome annotation databases
EnsemblPlantsi | AT5G63380.1; AT5G63380.1; AT5G63380 |
GeneIDi | 836457 |
Gramenei | AT5G63380.1; AT5G63380.1; AT5G63380 |
KEGGi | ath:AT5G63380 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY250835 mRNA Translation: AAP03018.1 AY376731 mRNA Translation: AAQ86590.1 AB023035, AB007649 Genomic DNA Translation: BAB10742.1 CP002688 Genomic DNA Translation: AED97741.1 AY136459 mRNA Translation: AAM97124.1 BT010394 mRNA Translation: AAQ56837.1 |
RefSeqi | NP_201143.1, NM_125733.3 |
3D structure databases
SMRi | Q84P23 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G63380.1 |
Chemistry databases
SwissLipidsi | SLP:000001792 |
PTM databases
iPTMneti | Q84P23 |
Proteomic databases
PaxDbi | Q84P23 |
PRIDEi | Q84P23 |
ProteomicsDBi | 245107 |
Genome annotation databases
EnsemblPlantsi | AT5G63380.1; AT5G63380.1; AT5G63380 |
GeneIDi | 836457 |
Gramenei | AT5G63380.1; AT5G63380.1; AT5G63380 |
KEGGi | ath:AT5G63380 |
Organism-specific databases
Araporti | AT5G63380 |
TAIRi | locus:2158559, AT5G63380 |
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
HOGENOMi | CLU_000022_59_2_1 |
InParanoidi | Q84P23 |
OMAi | EYRIICT |
OrthoDBi | 683933at2759 |
PhylomeDBi | Q84P23 |
Enzyme and pathway databases
BioCyci | ARA:AT5G63380-MONOMER |
SABIO-RKi | Q84P23 |
Miscellaneous databases
PROi | PR:Q84P23 |
Gene expression databases
ExpressionAtlasi | Q84P23, baseline and differential |
Genevisiblei | Q84P23, AT |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 4CLL9_ARATH | |
Accessioni | Q84P23Primary (citable) accession number: Q84P23 Secondary accession number(s): Q9FGW4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 11, 2007 | |
Last modified: | February 23, 2022 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families