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Protein

E3 ubiquitin-protein ligase SINAT3

Gene

SINAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi121Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi140Zinc 1By similarity1
Metal bindingi144Zinc 1By similarity1
Metal bindingi151Zinc 2By similarity1
Metal bindingi158Zinc 2By similarity1
Metal bindingi170Zinc 2By similarity1
Metal bindingi175Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 99RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri116 – 176SIAH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SINAT3Curated (EC:2.3.2.27By similarity)
Alternative name(s):
RING-type E3 ubiquitin transferase SINAT3Curated
Seven in absentia homolog 3Curated
Gene namesi
Name:SINAT31 Publication
Ordered Locus Names:At3g61790
ORF Names:F15G16.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G61790
TAIRilocus:2076810 AT3G61790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561821 – 326E3 ubiquitin-protein ligase SINAT3Add BLAST326

Proteomic databases

PaxDbiQ84JL3
PRIDEiQ84JL3

Expressioni

Inductioni

Induced by drought stress, salt stress, osmotic shock and abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiQ84JL3 baseline and differential
GenevisibleiQ84JL3 AT

Interactioni

Subunit structurei

Interacts with SINAT6 (PubMed:24350984). Interacts with WAV3 (PubMed:22122664).2 Publications

Protein-protein interaction databases

BioGridi10666, 18 interactors
IntActiQ84JL3, 18 interactors
STRINGi3702.AT3G61790.1

Structurei

3D structure databases

ProteinModelPortaliQ84JL3
SMRiQ84JL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 306SBDAdd BLAST194

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 99RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri116 – 176SIAH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000231487
InParanoidiQ84JL3
KOiK04506
OMAiEACEFRP
OrthoDBiEOG09360H9T
PhylomeDBiQ84JL3

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

Q84JL3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLDSMDCTS TMDVTDDEEI HQDRHSYASV SKHHHTNNNT TNVNAAASGL
60 70 80 90 100
LPTTTSVHEL LECPVCTNSM YPPIHQCHNG HTLCSTCKAR VHNRCPTCRQ
110 120 130 140 150
ELGDIRCLAL EKVAESLELP CKHMSLGCPE IFPYYSKLKH ETVCNFRPYS
160 170 180 190 200
CPYAGSECSV TGDIPFLVAH LRDDHKVDMH SGCTFNHRYV KSNPREVENA
210 220 230 240 250
TWMLTVFHCF GQYFCLHFEA FQLGMAPVYM AFLRFMGDET EARNYNYSLE
260 270 280 290 300
VGGYGRKLIW EGTPRSVRDS HRKVRDSHDG LIIQRNMALF FSGGDRKELK
310 320
LRVTGRIWKE QQQSGEGGGA CIPNLS
Length:326
Mass (Da):36,947
Last modified:June 1, 2003 - v1
Checksum:i96653A1E8EC7F9FA
GO

Sequence cautioni

The sequence CAB71109 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228V → F in AAM61286 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132959 Genomic DNA Translation: CAB71109.1 Different initiation.
CP002686 Genomic DNA Translation: AEE80257.1
BT003967 mRNA Translation: AAO42011.1
BT005079 mRNA Translation: AAO50612.1
AY084712 mRNA Translation: AAM61286.1
PIRiT47971
RefSeqiNP_567118.1, NM_116044.4
UniGeneiAt.21009

Genome annotation databases

EnsemblPlantsiAT3G61790.1; AT3G61790.1; AT3G61790
GeneIDi825352
GrameneiAT3G61790.1; AT3G61790.1; AT3G61790
KEGGiath:AT3G61790

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132959 Genomic DNA Translation: CAB71109.1 Different initiation.
CP002686 Genomic DNA Translation: AEE80257.1
BT003967 mRNA Translation: AAO42011.1
BT005079 mRNA Translation: AAO50612.1
AY084712 mRNA Translation: AAM61286.1
PIRiT47971
RefSeqiNP_567118.1, NM_116044.4
UniGeneiAt.21009

3D structure databases

ProteinModelPortaliQ84JL3
SMRiQ84JL3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10666, 18 interactors
IntActiQ84JL3, 18 interactors
STRINGi3702.AT3G61790.1

Proteomic databases

PaxDbiQ84JL3
PRIDEiQ84JL3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G61790.1; AT3G61790.1; AT3G61790
GeneIDi825352
GrameneiAT3G61790.1; AT3G61790.1; AT3G61790
KEGGiath:AT3G61790

Organism-specific databases

AraportiAT3G61790
TAIRilocus:2076810 AT3G61790

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000231487
InParanoidiQ84JL3
KOiK04506
OMAiEACEFRP
OrthoDBiEOG09360H9T
PhylomeDBiQ84JL3

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q84JL3

Gene expression databases

ExpressionAtlasiQ84JL3 baseline and differential
GenevisibleiQ84JL3 AT

Family and domain databases

Gene3Di2.60.210.10, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSINA3_ARATH
AccessioniPrimary (citable) accession number: Q84JL3
Secondary accession number(s): Q8LFQ3, Q9M359
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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