Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 102 (02 Dec 2020)
Sequence version 1 (01 Jun 2003)
Previous versions | rss
Add a publicationFeedback
Protein

Serine protease EatA

Gene

eatA

Organism
Escherichia coli O78:H11 (strain H10407 / ETEC)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Autotransporter serine protease probably involved in virulence.1 Publication

Miscellaneous

Leads to accelerated virulence in the rabbit ileal loop model of infection. Nevertheless, the relationship between the enzymatic and potential virulence functions is uncertain.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by phenylmethylsulfonyl fluoride.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei134Charge relay system1
Active sitei162Charge relay system1
Active sitei267Charge relay system1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processVirulence

Protein family/group databases

MEROPS protease database

More...
MEROPSi
S06.009

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Serine protease EatA (EC:3.4.21.-)
Alternative name(s):
Autotransporter protein EatA
ETEC autotransporter A
Cleaved into the following 2 chains:
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:eatA
Ordered Locus Names:ETEC_p948_0020
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pCS11 Publication
Plasmid p9481 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli O78:H11 (strain H10407 / ETEC)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri316401 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006877 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Plasmid p948

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi134H → R: Loss of activity. 1 Publication1
Mutagenesisi162D → A: Loss of activity. 1 Publication1
Mutagenesisi267S → G: Loss of activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 56Sequence analysisAdd BLAST56
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000002695257 – 1364Serine protease EatAAdd BLAST1308
ChainiPRO_000038759157 – 1098Secreted autotransporter protein EatAAdd BLAST1042
ChainiPRO_00000269531099 – 1364Autotransporter protein EatA translocatorSequence analysisAdd BLAST266

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei1098 – 1099CleavageSequence analysis2

Keywords - PTMi

Zymogen

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q84GK0

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini57 – 307Peptidase S6PROSITE-ProRule annotationAdd BLAST251
Domaini1098 – 1364AutotransporterPROSITE-ProRule annotationAdd BLAST267

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (Probable).Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000723_0_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
WVLTGYQ

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.160.20.20, 1 hit
2.40.128.130, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005546, Autotransporte_beta
IPR036709, Autotransporte_beta_dom_sf
IPR006315, OM_autotransptr_brl
IPR012332, P22_tailspike-like_C_sf
IPR011050, Pectin_lyase_fold/virulence
IPR009003, Peptidase_S1_PA
IPR000710, Peptidase_S6
IPR030396, Peptidase_S6_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03797, Autotransporter, 1 hit
PF02395, Peptidase_S6, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00921, IGASERPTASE

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00869, Autotransporter, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103515, SSF103515, 1 hit
SSF50494, SSF50494, 1 hit
SSF51126, SSF51126, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01414, autotrans_barl, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51208, AUTOTRANSPORTER, 1 hit
PS51691, PEPTIDASE_S6, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q84GK0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKVFSLKYS FLAKGFIAVS ELARRVSVKG KLKSASSIII SPITIAIVSY
60 70 80 90 100
APPSLAATVN ADISYQTFRD FAENKGAFIV GASNINIYDK NGVLVGVLDK
110 120 130 140 150
APMPDFSSAT MNTGTLPPGD HTLYSPQYVV TAKHVNGSDI MSFGHIQNNY
160 170 180 190 200
TVVGENNHNS LDIKIRRLNK IVTEVAPAEI SSVGAVNGAY QEGGRFKAFY
210 220 230 240 250
RLGGGLQYIK DKNGNLTPVY TNGGFLTGGT ISALSSYNNG QMITAPTGDI
260 270 280 290 300
FNPANGPLAN YLNKGDSGSP LFAYDSLDKK WVLVGVLSSG SEHGNNWVVT
310 320 330 340 350
TQDFLHQQPK HDFDKTISYD SEKGSLQWRY NKNSGVGTLS QESVVWDMHG
360 370 380 390 400
KKGGDLNAGK NLQFTGNNGE IILHDSIDQG AGYLQFFDNY TVTSLTDQTW
410 420 430 440 450
TGGGIITEKG VNVLWQVNGV NDDNLHKVGE GTLTVNGKGV NNGGLKVGDG
460 470 480 490 500
TVILNQRPDD NGHKQAFSSI NISSGRATVI LSDANQVNPD KISWGYRGGT
510 520 530 540 550
LDLNGNNVNF TRLQAADYGA IVSNNNKNKS ELTLKLQTLN ENDISVDVKT
560 570 580 590 600
YEVFGGHGSP GDLYYVPASN TYFILKSKAY GPFFSDLDNT NVWQNVGHDR
610 620 630 640 650
DKAIQIVKQQ KIGESSQPYM FHGQLNGYMD VNIHPLSGKD VLTLDGSVNL
660 670 680 690 700
PEGVITKKSG TLIFQGHPVI HAGMTTSAGQ SDWENRQFTM DKLRLDAATF
710 720 730 740 750
HLSRNAHMQG DISAANGSTV ILGSSRVFTD KNDGTGNAVS SVEGSSIATT
760 770 780 790 800
AGDQSYYSGN VLLENHSSLE VRENFTGGIE AYDSSVSVTS QNAIFDHVGS
810 820 830 840 850
FVNSSLLLEK GAKLTAQSGI FTNNTMKIKE NASLTLTGIP SVGKPGYYSP
860 870 880 890 900
VTSTTEGIHL GERASLSVKN MGYLSSNITA ENSAAIINLG DSNATIGKTD
910 920 930 940 950
SPLFSTLMRG YNAVLQGNIM GPQSSVNMNN ALWHSDRNSE LKELKANDSQ
960 970 980 990 1000
IELGVRGHFA KLRVKELIAS NSVFLVHANN SQADQLNVTD KLQGSNNTIL
1010 1020 1030 1040 1050
VDFFNKAANG TNVTLITAPK GSDENTFKAG TQQIGFSNIT PEIRTENTDT
1060 1070 1080 1090 1100
ATQWVLTGYQ SVADARASKI ATDFMDSGYK SFLTEVNNLN KRMGDLRDSQ
1110 1120 1130 1140 1150
GDAGGWARIM NGTGSGESGY RDNYTHVQIG ADRKHELNGI DLFTGALLTY
1160 1170 1180 1190 1200
TDNNASSQAF SGKTKSLGGG VYASGLFESG AYFDLIGKYL HHDNRYTLNF
1210 1220 1230 1240 1250
ASLGERSYTS HSLYAGAEIG YRYHMSENTW VEPQMELVYG SVSGKSFNWK
1260 1270 1280 1290 1300
DQGMQLSMKD KDYHPLIGRT GVDVGRAFSG DTWKVTVRAG LGYQFDLLAN
1310 1320 1330 1340 1350
GETVLQDASG KKHFKGEKDS RMLMNVGTNV EVKDNMRFGL ELEKSAFGRY
1360
NIDNSINANF RYYF
Length:1,364
Mass (Da):147,696
Last modified:June 1, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i33B341FDAB6859E2
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY163491 Genomic DNA Translation: AAO17297.1
FN649418 Genomic DNA Translation: CBJ04449.1

NCBI Reference Sequences

More...
RefSeqi
WP_001045019.1, NC_017724.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CBJ04449; CBJ04449; ETEC_p948_0020

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
elh:ETEC_p948_0020

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY163491 Genomic DNA Translation: AAO17297.1
FN649418 Genomic DNA Translation: CBJ04449.1
RefSeqiWP_001045019.1, NC_017724.1

3D structure databases

SMRiQ84GK0
ModBaseiSearch...

Protein family/group databases

MEROPSiS06.009

Genome annotation databases

EnsemblBacteriaiCBJ04449; CBJ04449; ETEC_p948_0020
KEGGielh:ETEC_p948_0020

Phylogenomic databases

HOGENOMiCLU_000723_0_0_6
OMAiWVLTGYQ

Family and domain databases

Gene3Di2.160.20.20, 1 hit
2.40.128.130, 1 hit
InterProiView protein in InterPro
IPR005546, Autotransporte_beta
IPR036709, Autotransporte_beta_dom_sf
IPR006315, OM_autotransptr_brl
IPR012332, P22_tailspike-like_C_sf
IPR011050, Pectin_lyase_fold/virulence
IPR009003, Peptidase_S1_PA
IPR000710, Peptidase_S6
IPR030396, Peptidase_S6_dom
PfamiView protein in Pfam
PF03797, Autotransporter, 1 hit
PF02395, Peptidase_S6, 1 hit
PRINTSiPR00921, IGASERPTASE
SMARTiView protein in SMART
SM00869, Autotransporter, 1 hit
SUPFAMiSSF103515, SSF103515, 1 hit
SSF50494, SSF50494, 1 hit
SSF51126, SSF51126, 1 hit
TIGRFAMsiTIGR01414, autotrans_barl, 1 hit
PROSITEiView protein in PROSITE
PS51208, AUTOTRANSPORTER, 1 hit
PS51691, PEPTIDASE_S6, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEATA_ECOH1
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q84GK0
Secondary accession number(s): E3PP92
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: December 2, 2020
This is version 102 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Plasmid
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again