UniProtKB - Q84710 (P1_PEMVW)
Protein
Protein P1
Gene
ORF1
Organism
Pea enation mosaic virus-1 (strain WSG) (PEMV-1)
Status
Functioni
Precursor from which the VPg molecule is probably released at the onset of the RNA synthesis. Essential for virus replication (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 366 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 396 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 465 | For protease activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
- serine-type exopeptidase activity Source: InterPro
GO - Biological processi
- viral process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, RNA-binding, Serine protease |
Names & Taxonomyi
Protein namesi | Recommended name: Protein P1Alternative name(s): Genome-linked protein precursor ORF1 protein Cleaved into the following 3 chains: |
Gene namesi | ORF Names:ORF1 |
Organismi | Pea enation mosaic virus-1 (strain WSG) (PEMV-1) |
Taxonomic identifieri | 693989 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Tolucaviricetes › Tolivirales › Luteoviridae › Enamovirus › |
Virus hosti | Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827] Lathyrus odoratus (Sweet pea) [TaxID: 3859] Lens culinaris (Lentil) (Cicer lens) [TaxID: 3864] Medicago arabica [TaxID: 70936] Pisum sativum (Garden pea) [TaxID: 3888] Trifolium incarnatum (Crimson clover) [TaxID: 60916] Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906] Vicia sativa (Spring vetch) (Tare) [TaxID: 3908] |
Proteomesi |
|
Subcellular locationi
- Membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 194 – 214 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 218 – 235 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 240 – 260 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Sequence analysisAdd BLAST | 33 | |
ChainiPRO_0000390915 | 34 – 760 | Protein P1Add BLAST | 727 | |
ChainiPRO_0000390916 | 316 – 515 | Serine proteaseSequence analysisAdd BLAST | 200 | |
ChainiPRO_0000390917 | 516 – 543 | VPgSequence analysisAdd BLAST | 28 | |
ChainiPRO_0000391318 | 544 – 760 | P1-C25Sequence analysisAdd BLAST | 217 |
Post-translational modificationi
Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the VPg protein (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 315 – 316 | Cleavage; by viral serine proteaseSequence analysis | 2 | |
Sitei | 515 – 516 | Cleavage; by viral serine proteaseSequence analysis | 2 | |
Sitei | 543 – 544 | CleavageSequence analysis | 2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 318 – 515 | Peptidase S39PROSITE-ProRule annotationAdd BLAST | 198 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 132 – 135 | Poly-Leu | 4 | |
Compositional biasi | 505 – 508 | Poly-Ser | 4 |
Domaini
The C-terminus part of protein P1 and P1-C25 displays RNA-binding properties.By similarity
Sequence similaritiesi
Belongs to the peptidase S39B family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR018019, Luteovirus_Orf2 IPR000382, Peptidase_S39B_luteovirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit |
PRINTSi | PR00913, LVIRUSORF2 |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Protein P1 (identifier: Q84710-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASFLKPVNS QGLWLSLLLA ITYLFLLPSA GQSLDPSGIG LAAGCSQSQG
60 70 80 90 100
GISSFAALPR PCNDSVCTLP DLGWSCQRTA QDTANQQQSP FNHTGHFLTT
110 120 130 140 150
SGWTWPNWTC SPSQCQLLIH LPTWQIVKQD FLLLLKEWDL LTMCQRCSDL
160 170 180 190 200
LTKTPGFILR FAGETLILVA NLIEFVLVSW SLWLCSVLVY VAQAVPGKFL
210 220 230 240 250
LYMAAFCTTF WAWPRETASS LIRIVTTPLT LIGFLNKTGI GLISHCLALT
260 270 280 290 300
WNMFMTWSLL PWVTLMKMMK ILITSSRVLT RSGRPKRTSS KSLKHKLKIS
310 320 330 340 350
RAIQKKQGKK TPVEERTIPG VQIKKLREDP PKGVILRCTD QFGDHVGYAS
360 370 380 390 400
AVKLEKGQTG IVLPIHVWTD TVYINGPNGK LKMADFTALY EVTNHDSLIM
410 420 430 440 450
TSAMAGWGSI LGVRPRPLTT IDAVKLKNYS LFTERDGKWY VQAAKCIAPA
460 470 480 490 500
EGMFRVVSDT RPGDSGLPLF DMKMNVVAVH RGTWPSERFP ENRAFAILPV
510 520 530 540 550
PDLTSSSSPK FTGCETYSEA ETAYEMADNF SDGEEILIRT KGQSYRTFIG
560 570 580 590 600
SNKVALLSIR KLEEELSRGP IGLWADDTED DESAPRRSGN GLFRSTPEKQ
610 620 630 640 650
SQAKTPSPKV EESAAPPPAP RAEKVRHVRR SEMTPEQKRA DNLRRRKAKA
660 670 680 690 700
AKKTPSTPPK KSKDKAPTLS QVAELVEKAV RAALTVQPRR SRASSKISIG
710 720 730 740 750
GRNPGRKPQV SIQLDPVPSQ STSVPPKDSQ AGESAWLGPR RSYRPVQKST
760
VGQKQEPRRN
Note: Produced by conventional translation.
Isoform RNA-directed RNA polymerase (identifier: P29154-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P29154.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04573 Genomic RNA Translation: AAA72300.1 |
PIRi | JQ1383 |
RefSeqi | NP_619736.1, NC_003629.1 |
Genome annotation databases
GeneIDi | 940254 |
KEGGi | vg:940254 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L04573 Genomic RNA Translation: AAA72300.1 |
PIRi | JQ1383 |
RefSeqi | NP_619736.1, NC_003629.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
GeneIDi | 940254 |
KEGGi | vg:940254 |
Family and domain databases
InterProi | View protein in InterPro IPR018019, Luteovirus_Orf2 IPR000382, Peptidase_S39B_luteovirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit |
PRINTSi | PR00913, LVIRUSORF2 |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P1_PEMVW | |
Accessioni | Q84710Primary (citable) accession number: Q84710 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 19, 2010 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families