Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 86 (02 Jun 2021)
Sequence version 2 (31 May 2011)
Previous versions | rss
Add a publicationFeedback
Protein

Replicase large subunit

Gene

rep

Organism
Peanut clump virus (isolate 87/TGTA2) (PCV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

is an RNA-dependent RNA polymerase active in viral RNA replication.

By similarity

is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential).

Curated

Miscellaneous

The replicase large subunit is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for 1145-Lys and 1147-Arg, this results in the addition of the RdRp region.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi856 – 863ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Methyltransferase, Nucleotidyltransferase, RNA-binding, RNA-directed RNA polymerase, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
RNA-directed RNA polymerase
Cleaved into the following chain:
Replicase small subunit (EC:2.1.1.-, EC:2.7.7.-, EC:3.6.4.13)
Alternative name(s):
Methyltransferase/RNA helicase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rep
ORF Names:ORF1-1bis
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPeanut clump virus (isolate 87/TGTA2) (PCV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri652837 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraeKitrinoviricotaAlsuviricetesMartelliviralesVirgaviridaePecluvirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiArachis hypogaea (Peanut) [TaxID: 3818]
Setaria italica (Foxtail millet) (Panicum italicum) [TaxID: 4555]
Sorghum arundinaceum [TaxID: 91525]
Sorghum bicolor (Sorghum) (Sorghum vulgare) [TaxID: 4558]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001668 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004091461 – 1672Replicase large subunitAdd BLAST1672
ChainiPRO_00004091471 – 1145Replicase small subunitAdd BLAST1145

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q84687

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a large and a small subunit.

By similarity

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini85 – 306Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST222
Domaini823 – 982(+)RNA virus helicase ATP-bindingAdd BLAST160
Domaini983 – 1145(+)RNA virus helicase C-terminalAdd BLAST163
Domaini1406 – 1519RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni64 – 801MethyltransferaseSequence analysisAdd BLAST738
Regioni853 – 1113HelicaseSequence analysisAdd BLAST261

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili547 – 575Sequence analysisAdd BLAST29

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027351, (+)RNA_virus_helicase_core_dom
IPR002588, Alphavirus-like_MT_dom
IPR043502, DNA/RNA_pol_sf
IPR027417, P-loop_NTPase
IPR007094, RNA-dir_pol_PSvirus
IPR001788, Tymovirus_RNA-dep_RNA_pol
IPR013664, Virgavirus_MeTrfase_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00978, RdRP_2, 1 hit
PF01443, Viral_helicase1, 1 hit
PF01660, Vmethyltransf, 1 hit
PF08456, Vmethyltransf_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 1 hit
SSF56672, SSF56672, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51743, ALPHAVIRUS_MT, 1 hit
PS51657, PSRV_HELICASE, 1 hit
PS50507, RDRP_SSRNA_POS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q84687-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQSNIYSDM IDRYVNDETV VQNLFMTASS TTKSLLHDVI ADRAANHLKS
60 70 80 90 100
LEKKQKQKEL VDVRRVLSSE SLNALCALYP EFHVITSNCE RGSHTMAAVC
110 120 130 140 150
RTLETLYIKS LLPVKHTVVW DVGGNWLTHV KYASDQNVHC CCPLLDYRDA
160 170 180 190 200
MRKQERLLSL ELFARNGKEK TEEFDECYKQ IKAFELQRRA ALNAGTAASC
210 220 230 240 250
NNKYCCDVFQ DCAYEPEEGK MRVAMGVHSV YDMTLQDLVN GLERKGIEHF
260 270 280 290 300
IGCFLFSPKL LLGQEEGELP FVNGRFKVKK GKIRFFFLDD TTHGYEHDLN
310 320 330 340 350
DYLDYIKKSF VVAKCGHVYM LELFSVRGDT IFFKLYDVTE YSLSRTRLLS
360 370 380 390 400
SLLPTRDHVF KAMPIPNTEE VIVPLYWIEN GEVVVERRYL PKSLVCRGME
410 420 430 440 450
WLMRNKANAL QYETLLNYLV STNVSAVFNG CQVREGLKTD PWILCKLAMT
460 470 480 490 500
LLVREEFNRE KQKKILEMLR MSGSEQISLK SIFRGVFVKF FGSVSLKARV
510 520 530 540 550
LKTVARWCGV EFGSMKYDVE VLPLYVEIED SLCLWRKGRL EGIESYDMKD
560 570 580 590 600
EVLRYNEKKQ DVERLTRSLE QKVNDGSASD TSSKLKKVAA FAVLASNKDT
610 620 630 640 650
PLGRFIASRS LSSVSKRSSS SDASHSGSGL SASEKVVSSE EIASPQISVS
660 670 680 690 700
ESCVEEVPPM IIPYTVKKRW ADYSSDSSED VVAGEDFMSP VSTLVLRRRL
710 720 730 740 750
PAPPAYPDDV QEAACLEYLW YLKCKIICDF SAMYSVVCDF KDQLLHDGRC
760 770 780 790 800
EFPKNAFFLK VGEETKWAMK RPTSQQVGHQ YCVKFSENDD HMELVPVAWS
810 820 830 840 850
KHDDEIRGIY PQGLGDGWYM FSDLTYLMNE WLIFNKLVLV YPSLPKTQLK
860 870 880 890 900
VRLIDGVPGC GKSTWILSNC DLDRQIVLAE GREATDDLRR RFTEKGFPKK
910 920 930 940 950
RCEERVRTVH SFMLKPLSRR FNSFHFDEAL MAHAGMIYIC GRMLNAREVI
960 970 980 990 1000
CQGDSKQIPF INRVEQISLK YSSFNVVERE HVRKTYRCPL DIVYYLNKKK
1010 1020 1030 1040 1050
YYRGDDIVGY SKTTHSVDTK SKSSGFTSLV KLPKEPVHYL TFLQAEKEEV
1060 1070 1080 1090 1100
SKHLAGVKGA TVSTVHEAQG KTFERVNLVR LKMTDNELYP GGAKSEPYTI
1110 1120 1130 1140 1150
VGLTRHTRSL VYYSVVEDRL YEDISALKDV MEDQLLKCSF SEQTKXRFGS
1160 1170 1180 1190 1200
KFESIVVADR KVMAPDVGDL VTIQDLYDRT FPGNSTLDSS FDGYTVASSD
1210 1220 1230 1240 1250
LELEISNCKI APNKSIRGFQ EKICFSPKLR TAMPEKRQGS FAESILALRK
1260 1270 1280 1290 1300
RNMACPRLQE SVNEHQIIED TIAKAFSCYF TDSFVDQSPL ITDESALRWW
1310 1320 1330 1340 1350
EKQSTTAKNQ MLADWRTLDQ IDVCTYNFMI KNDVKPKLDL TPQSEYAALQ
1360 1370 1380 1390 1400
TVVYPEKIVN GLFGPVIKEI NERVLSALKP NVFVNTRMTS EELSRTAEYL
1410 1420 1430 1440 1450
YPGDEFEVVE IDFSKYDKSK TSLHIRMVIK LYEQFGLNGY MKYLWEKSQT
1460 1470 1480 1490 1500
QTVVKDRNYG VEAYILYQQK SGNCDTYGSN TYSSMFALLD CLPMEKAVYS
1510 1520 1530 1540 1550
IFGGDDSLIL FPKGTIINDP CGRLASLWNF DCKSMKFRVP AFCGKFLIPV
1560 1570 1580 1590 1600
GGRYRFEPDP MKLITKLGNK TIAIEGKTKA EVRKNGSKLL SEIYVSICDN
1610 1620 1630 1640 1650
YKNYDDARVL DALAYALVDR YKPDICPKAA LYSLSKFLKS FESFTELFDI
1660 1670
YFNGERLAFD FVPVKVRKDY EW
Length:1,672
Mass (Da):191,375
Last modified:May 31, 2011 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF6E0D9E37273DDB3
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA55335 differs from that shown. Reason: Erroneous translation. Readthrough in 1146.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X78602 Genomic RNA Translation: CAA55335.1 Sequence problems.

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78602 Genomic RNA Translation: CAA55335.1 Sequence problems.

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

Proteomic databases

PRIDEiQ84687

Family and domain databases

InterProiView protein in InterPro
IPR027351, (+)RNA_virus_helicase_core_dom
IPR002588, Alphavirus-like_MT_dom
IPR043502, DNA/RNA_pol_sf
IPR027417, P-loop_NTPase
IPR007094, RNA-dir_pol_PSvirus
IPR001788, Tymovirus_RNA-dep_RNA_pol
IPR013664, Virgavirus_MeTrfase_C
PfamiView protein in Pfam
PF00978, RdRP_2, 1 hit
PF01443, Viral_helicase1, 1 hit
PF01660, Vmethyltransf, 1 hit
PF08456, Vmethyltransf_C, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit
SSF56672, SSF56672, 1 hit
PROSITEiView protein in PROSITE
PS51743, ALPHAVIRUS_MT, 1 hit
PS51657, PSRV_HELICASE, 1 hit
PS50507, RDRP_SSRNA_POS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRDRP_PCV87
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q84687
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: June 2, 2021
This is version 86 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again