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UniProtKB - Q83WC3 (SDMT_APHHA)
Protein
Sarcosine/dimethylglycine N-methyltransferase
Gene
N/A
Organism
Aphanothece halophytica
Status
Functioni
Catalyzes the methylation of sarcosine and dimethylglycine to dimethylglycine and betaine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor.
1 PublicationCatalytic activityi
- 2 S-adenosyl-L-methionine + sarcosine = glycine betaine + 2 H+ + 2 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1571 Publication
Activity regulationi
Inhibited by n-butylic acid and S-adenosyl-L-homocysteine.1 Publication
Kineticsi
- KM=0.18 mM for S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees Celsius)1 Publication
- KM=0.5 mM for dimethylglycine (at pH 8.8 and at 37 degrees Celsius)1 Publication
- Vmax=0.47 µmol/min/mg enzyme (at pH 8.8 and at 37 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 8.8. The activities remain high at more alkaline pH but decrease sharply at the acidic side of the optimal pH.1 Publication
: betaine biosynthesis via glycine pathway Pathwayi
This protein is involved in step 2 and 3 of the subpathway that synthesizes betaine from glycine.1 Publication This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 171 | Important for dimethylglycine bindingCurated | 1 | |
Sitei | 172 | Important for dimethylglycine bindingCurated | 1 |
GO - Molecular functioni
- dimethylglycine N-methyltransferase activity Source: UniProtKB
- sarcosine N-methyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- glycine betaine biosynthetic process from glycine Source: UniProtKB
- methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.157, 383 |
UniPathwayi | UPA00530;UER00382 UPA00530;UER00383 |
Names & Taxonomyi
Protein namesi | Recommended name: Sarcosine/dimethylglycine N-methyltransferase (EC:2.1.1.157)Alternative name(s): Dimethylglycine N-methyltransferase |
Organismi | Aphanothece halophytica |
Taxonomic identifieri | 72020 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Aphanothecaceae › Aphanothece |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 171 | P → Q: The Vmax value is about 27% of the wild-type when dimethylglycine is used as a methyl group acceptor. Glycine and sarcosine are ineffective as methyl group acceptors. The affinity for dimethylglycine decreases about 15-fold, and 2-fold for AdoMet. Almost inactive; when associated with R-172. 1 Publication | 1 | |
Mutagenesisi | 172 | M → R: Almost inactive; when associated with Q-171. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000412535 | 1 – 277 | Sarcosine/dimethylglycine N-methyltransferaseAdd BLAST | 277 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the methyltransferase superfamily.Curated
Family and domain databases
InterProi | View protein in InterPro IPR013216, Methyltransf_11 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08241, Methyltransf_11, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
i Sequence
Sequence statusi: Complete.
Q83WC3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKADAVAKQ AQDYYDSGSA DGFYYRIWGG EDLHIGIYNT PDEPIYDASV
60 70 80 90 100
RTVSRICDKI KNWPAGTKVL DLGAGYGGSA RYMAKHHGFD VDCLNISLVQ
110 120 130 140 150
NERNRQMNQE QGLADKIRVF DGSFEELPFE NKSYDVLWSQ DSILHSGNRR
160 170 180 190 200
KVMEEADRVL KSGGDFVFTD PMQTDNCPEG VLEPVLARIH LDSLGSVGFY
210 220 230 240 250
RQVAEELGWE FVEFDEQTHQ LVNHYSRVLQ ELEAHYDQLQ PECSQEYLDR
260 270
MKVGLNHWIN AGKSGYMAWG ILKFHKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB094498 Genomic DNA Translation: BAC56940.1 |
Genome annotation databases
KEGGi | ag:BAC56940 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB094498 Genomic DNA Translation: BAC56940.1 |
3D structure databases
SMRi | Q83WC3 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:BAC56940 |
Enzyme and pathway databases
UniPathwayi | UPA00530;UER00382 UPA00530;UER00383 |
BRENDAi | 2.1.1.157, 383 |
Family and domain databases
InterProi | View protein in InterPro IPR013216, Methyltransf_11 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF08241, Methyltransf_11, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SDMT_APHHA | |
Accessioni | Q83WC3Primary (citable) accession number: Q83WC3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 21, 2011 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families