UniProtKB - Q83RE8 (YDFG_SHIFL)
NADP-dependent 3-hydroxy acid dehydrogenase YdfG
ydfG
Functioni
NADP-dependent dehydrogenase with broad substrate specificity acting on 3-hydroxy acids. Catalyzes the NADP-dependent oxidation of L-allo-threonine to L-2-amino-3-keto-butyrate, which is spontaneously decarboxylated into aminoacetone. Also acts on D-threonine, L-serine, D-serine, D-3-hydroxyisobutyrate, L-3-hydroxyisobutyrate, D-glycerate and L-glycerate. Able to catalyze the reduction of the malonic semialdehyde to 3-hydroxypropionic acid. YdfG is apparently supplementing RutE, the presumed malonic semialdehyde reductase involved in pyrimidine degradation since both are able to detoxify malonic semialdehyde.
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 81 | NADP; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 134 | SubstrateBy similarity | 1 | |
Active sitei | 147 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 147 | NADPBy similarity | 1 | |
Binding sitei | 151 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 7 – 12 | NADPBy similarity | 6 | |
Nucleotide bindingi | 32 – 33 | NADPBy similarity | 2 | |
Nucleotide bindingi | 54 – 55 | NADPBy similarity | 2 | |
Nucleotide bindingi | 177 – 185 | NADPBy similarity | 9 |
GO - Molecular functioni
- 3-hydroxypropionate dehydrogenase (NADP+) activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ydfGBy similarity Ordered Locus Names:SF1556, S1681 |
Organismi | Shigella flexneri |
Taxonomic identifieri | 623 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Shigella |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054830 | 1 – 248 | NADP-dependent 3-hydroxy acid dehydrogenase YdfGAdd BLAST | 248 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 198214.SF1556 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_010194_2_10_6 |
OMAi | GMDERTF |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MIVLVTGATA GFGECITRRF IQQGHKVIAT GRRQERLQEL TDELGDNLYI
60 70 80 90 100
AQLDVRNRAA IEEMLASLPA EWSNIDILVN NAGLALGMEP AHKASVEDWE
110 120 130 140 150
TMIDTNNKGL VYMTRAVLPG MVERNHGHII NIGSTAGSWP YAGGNVYGAT
160 170 180 190 200
KAFVRQFSLN LRTDLHGTTV RVTDIEPGLV GGTEFSNVRF KGDDGKAEKT
210 220 230 240
YQNTVALTPE DVSEAVWWVS TLPAHVNINT LEMMPVTQSY AGLNVHRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005674 Genomic DNA Translation: AAN43145.2 AE014073 Genomic DNA Translation: AAP17037.1 |
RefSeqi | NP_707438.2, NC_004337.2 |
Genome annotation databases
EnsemblBacteriai | AAN43145; AAN43145; SF1556 AAP17037; AAP17037; S1681 |
GeneIDi | 1024772 |
KEGGi | sfl:SF1556 sfx:S1681 |
PATRICi | fig|198214.7.peg.1838 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005674 Genomic DNA Translation: AAN43145.2 AE014073 Genomic DNA Translation: AAP17037.1 |
RefSeqi | NP_707438.2, NC_004337.2 |
3D structure databases
SMRi | Q83RE8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 198214.SF1556 |
Genome annotation databases
EnsemblBacteriai | AAN43145; AAN43145; SF1556 AAP17037; AAP17037; S1681 |
GeneIDi | 1024772 |
KEGGi | sfl:SF1556 sfx:S1681 |
PATRICi | fig|198214.7.peg.1838 |
Phylogenomic databases
HOGENOMi | CLU_010194_2_10_6 |
OMAi | GMDERTF |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | YDFG_SHIFL | |
Accessioni | Q83RE8Primary (citable) accession number: Q83RE8 Secondary accession number(s): Q7UCH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | February 23, 2022 | |
This is version 99 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families