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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. Fructose-1,6-bisphosphate aldolase (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATP; via amide nitrogenUniRule annotation1
Metal bindingi106Magnesium; catalyticUniRule annotation1
Active sitei131Proton acceptorUniRule annotation1
Binding sitei158Allosteric activator ADPUniRule annotation1
Binding sitei166Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei215Allosteric activator ADPUniRule annotation1
Binding sitei225SubstrateUniRule annotation1
Binding sitei248Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 76ATPUniRule annotation2
Nucleotide bindingi105 – 108ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TIGR
  • AMP binding Source: GO_Central
  • ATP binding Source: GO_Central
  • fructose-6-phosphate binding Source: GO_Central
  • identical protein binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • monosaccharide binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:CBU_0341
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000720551 – 326ATP-dependent 6-phosphofructokinaseAdd BLAST326

Proteomic databases

PRIDEiQ83EH9

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi227377.CBU_0341

Structurei

3D structure databases

ProteinModelPortaliQ83EH9
SMRiQ83EH9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 28Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni129 – 131Substrate bindingUniRule annotation3
Regioni173 – 175Substrate bindingUniRule annotation3
Regioni189 – 191Allosteric activator ADP bindingUniRule annotation3
Regioni216 – 218Allosteric activator ADP bindingUniRule annotation3
Regioni254 – 257Substrate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiGKLHSII

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q83EH9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEIKKLIVL TSGGDAPGMN AAIRAVVRTA LHYQFEVYGA TAGFAGLVNG
60 70 80 90 100
QVVPLNSRAV ANCIQRGGTI LKTGRFENFR FKAVRDKARE FLKKLQIDAM
110 120 130 140 150
IVLGGNGSFA GASKLYQEGG PQMIGIPCTI DNDIQGTDYC IGFDTACNTA
160 170 180 190 200
LQAIDKIRDT AFSHERNFLI EVMGRSSGFI AVNVGIAGGA EIIALPEFHV
210 220 230 240 250
DIDTLTQKIK KQHGKKSASI IVAAEANQPG HSFEVAKQIK EKTGIEYRVC
260 270 280 290 300
VLGHTQRGGT PTVKDRVLAS LMGAQAIEAL KKGLTEKMIA YQNGKIAVAP
310 320
LPDPDNGTRY FADEALLRVN NIICAM
Length:326
Mass (Da):35,012
Last modified:June 1, 2003 - v1
Checksum:iDC1EFC0038E87573
GO

Sequence cautioni

The sequence AAO89897 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO89897.2 Different initiation.
RefSeqiNP_819383.2, NC_002971.3

Genome annotation databases

EnsemblBacteriaiAAO89897; AAO89897; CBU_0341
GeneIDi1208223
KEGGicbu:CBU_0341
PATRICifig|227377.7.peg.335

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO89897.2 Different initiation.
RefSeqiNP_819383.2, NC_002971.3

3D structure databases

ProteinModelPortaliQ83EH9
SMRiQ83EH9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0341

Proteomic databases

PRIDEiQ83EH9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO89897; AAO89897; CBU_0341
GeneIDi1208223
KEGGicbu:CBU_0341
PATRICifig|227377.7.peg.335

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiGKLHSII

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_COXBU
AccessioniPrimary (citable) accession number: Q83EH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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