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Protein

DNA ligase

Gene

ligA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119NADUniRule annotation1
Active sitei121N6-AMP-lysine intermediateUniRule annotation1
Binding sitei142NADUniRule annotation1
Binding sitei179NADUniRule annotation1
Binding sitei296NADUniRule annotation1
Binding sitei320NADUniRule annotation1
Metal bindingi414ZincUniRule annotation1
Metal bindingi417ZincUniRule annotation1
Metal bindingi432ZincUniRule annotation1
Metal bindingi438ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 42NADUniRule annotation5
Nucleotide bindingi87 – 88NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-543-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:CBU_0542
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003132091 – 673DNA ligaseAdd BLAST673

Proteomic databases

PRIDEiQ83DZ6

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_0542

Structurei

3D structure databases

ProteinModelPortaliQ83DZ6
SMRiQ83DZ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini595 – 673BRCTUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C77 Bacteria
COG0272 LUCA
HOGENOMiHOG000218458
KOiK01972
OMAiFTAKSPR

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
IPR004149 Znf_DNAligase_C4
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PF03119 DNA_ligase_ZBD, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00278 HhH1, 4 hits
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

Q83DZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGVEVPAKI QKRIERLRQE INDHNYRYYV LSQPTIPDSV YDELFHELEK
60 70 80 90 100
LEKKYPETIT PSSPTQRVGA EPLKVFEPVH HEIPMLSLDN VFDEKGLRAF
110 120 130 140 150
DKRIRQRLKL DKPFEYVCEP KMDGVALSLL YENGELIRAA TRGDGYTGEN
160 170 180 190 200
VTQNTRTIAS VPLQLRGNDY PELVEIRGEV LMPREGFAKF NREAEKRGDK
210 220 230 240 250
TFANPRNAAS GSLRQLDPRI TAKRPLIFYG YLIGLLKGKD FPKNHCDVLK
260 270 280 290 300
WFKDWGIPVI SEIKVVGGIE GCLDYYEHLV KTREKMPFDI DGIVIKVNSL
310 320 330 340 350
QVQAELGFVS RAPRWAIAYK FPAQEKMTVV KAIEFQVGRT GAVTPVARLE
360 370 380 390 400
PVSVSGVTVS NATLHNFDEL YRKDVRVGDT VIVRRAGDVI PEVVGPILAK
410 420 430 440 450
RPKKAKLIKI PSRCPVCHAE VIKPEGEAVA RCVGGLYCRA QLRESIKHFA
460 470 480 490 500
SRRALDIEGL GDKLVELFIQ EKLIKDITGI YQLKKSAITA LPRMGEKSAE
510 520 530 540 550
NLLTAIEKSK KTTLPRFLYA LGIRGVGDTT ARTLARHFHE LDLLMKASIE
560 570 580 590 600
TLQEIRDIGP VAAENIHAFF HQKNNAELIN KLIHLGVHWP QEKAVVKSEI
610 620 630 640 650
AGKTFVLTGA LKSLTREEAE EKIERSGGKA TSSVSKNTDY VIVGENPGSK
660 670
YEKAKALGIS LIDEEAFLKL LKS
Length:673
Mass (Da):75,546
Last modified:December 16, 2008 - v2
Checksum:i413E79D01E6FD906
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO90088.2
RefSeqiNP_819574.2, NC_002971.3
WP_010957648.1, NC_002971.4

Genome annotation databases

EnsemblBacteriaiAAO90088; AAO90088; CBU_0542
GeneIDi1208427
KEGGicbu:CBU_0542
PATRICifig|227377.7.peg.535

Similar proteinsi

Entry informationi

Entry nameiDNLJ_COXBU
AccessioniPrimary (citable) accession number: Q83DZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 16, 2008
Last modified: February 28, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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