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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi489MagnesiumUniRule annotation1
Metal bindingi495MagnesiumUniRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • polyribonucleotide nucleotidyltransferase activity Source: TIGR
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA catabolic process Source: InterPro
  • RNA catabolic process Source: TIGR
  • RNA processing Source: InterPro

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-845-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:CBU_0852
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296121 – 696Polyribonucleotide nucleotidyltransferaseAdd BLAST696

Proteomic databases

PRIDEiQ83D87

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0852

Structurei

Secondary structure

1696
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi13 – 22Combined sources10
Beta strandi26 – 34Combined sources9
Beta strandi37 – 45Combined sources9
Beta strandi58 – 64Combined sources7
Helixi66 – 69Combined sources4
Helixi85 – 98Combined sources14
Helixi99 – 101Combined sources3
Beta strandi110 – 118Combined sources9
Helixi126 – 140Combined sources15
Beta strandi149 – 156Combined sources8
Beta strandi159 – 163Combined sources5
Helixi166 – 169Combined sources4
Beta strandi173 – 180Combined sources8
Beta strandi185 – 195Combined sources11
Helixi197 – 210Combined sources14
Helixi212 – 225Combined sources14
Helixi239 – 256Combined sources18
Helixi262 – 284Combined sources23
Beta strandi287 – 289Combined sources3
Helixi294 – 313Combined sources20
Beta strandi330 – 334Combined sources5
Beta strandi340 – 348Combined sources9
Beta strandi351 – 360Combined sources10
Helixi362 – 364Combined sources3
Beta strandi366 – 368Combined sources3
Beta strandi374 – 384Combined sources11
Helixi387 – 390Combined sources4
Helixi401 – 414Combined sources14
Helixi415 – 417Combined sources3
Turni421 – 423Combined sources3
Beta strandi427 – 436Combined sources10
Helixi441 – 455Combined sources15
Beta strandi465 – 474Combined sources10
Beta strandi477 – 483Combined sources7
Turni487 – 491Combined sources5
Beta strandi492 – 500Combined sources9
Beta strandi505 – 512Combined sources8
Helixi519 – 543Combined sources25
Beta strandi557 – 562Combined sources6
Helixi565 – 567Combined sources3
Helixi568 – 572Combined sources5
Helixi577 – 586Combined sources10
Beta strandi589 – 592Combined sources4
Beta strandi594 – 604Combined sources11
Helixi605 – 619Combined sources15
Turni655 – 657Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NBQX-ray2.91A/B/C1-696[»]
ProteinModelPortaliQ83D87
SMRiQ83D87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini556 – 615KHUniRule annotationAdd BLAST60
Domaini625 – 693S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218326
KOiK00962
OMAiRYMHNYN

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q83D87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIRKTFQY GKHEVTFETG EMARQATGAV VVRMGDTVLL VSVVAKKEAE
60 70 80 90 100
EGRDFFPLTV NYQEKTYAAG KIPGGYFKRE GRPTEKETLT SRLIDRPLRP
110 120 130 140 150
LFPKGFTNEV QVIATVLSVD SKVPTDIPAI LGASAAIGLS GIPFNGSLGA
160 170 180 190 200
ARVGYRGGEY LLNPSLDELK DSALDLVVAG TRDAVLMVES EAQELPESVM
210 220 230 240 250
LGAVLHGHQA MQVAIQAIAE FIQEAGGAKW EWEPPTVNTA LEKWVVEKSE
260 270 280 290 300
APLKKAYQIQ EKTARQAQIQ AIRDQLLADR AAEREGEENA VNEHELAVIF
310 320 330 340 350
HELERRIVRE QILTGQPRID GRDTKTVRPI TVKVGVLPRS HGSALFTRGE
360 370 380 390 400
TQALVVTTLG TERDAQSIDD LDGDRQEEFI FHYNFPPFCV GEVGFMSGPK
410 420 430 440 450
RREIGHGRLA KRAVVPVVPT LDKFPYVIRV VSEILESNGS SSMASVCGSS
460 470 480 490 500
LALMDAGVPT KAPVAGIAMG LIKENDKYAV LSDILGDEDH LGDMDFKVAG
510 520 530 540 550
TSNGVTALQM DIKIEGITKE IMEQALDQAK EGRLHILSIM NKVLDKPRSQ
560 570 580 590 600
VSDLAPQYVT MKINPEKIRD VIGKGGVVIR EITEATNCAI DISDDGTIKI
610 620 630 640 650
AAHTTEEGEA AKRRIEELTA EVELGKVYEG TVVKITDFGA FVQILPNTQG
660 670 680 690
LVHISQIAQE RVENVRDYLE EGQVIRVKVI EIDRQGRVRL SMKQID
Length:696
Mass (Da):76,331
Last modified:June 1, 2003 - v1
Checksum:i9E55E7C7CD00E52B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO90386.1
RefSeqiNP_819872.1, NC_002971.3
WP_010957848.1, NC_002971.4

Genome annotation databases

EnsemblBacteriaiAAO90386; AAO90386; CBU_0852
GeneIDi1208745
31483540
KEGGicbu:CBU_0852
PATRICifig|227377.7.peg.837

Similar proteinsi

Entry informationi

Entry nameiPNP_COXBU
AccessioniPrimary (citable) accession number: Q83D87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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