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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Pathwayi: glycine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glycine from L-serine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Serine hydroxymethyltransferase (glyA)
This subpathway is part of the pathway glycine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-serine, the pathway glycine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Pyridoxal phosphateUniRule annotation1
Binding sitei55Pyridoxal phosphateUniRule annotation1
Binding sitei57SubstrateUniRule annotation1
Binding sitei64SubstrateUniRule annotation1
Binding sitei65Pyridoxal phosphateUniRule annotation1
Binding sitei99Pyridoxal phosphateUniRule annotation1
Binding sitei121Substrate; via carbonyl oxygenUniRule annotation1
Binding sitei175Pyridoxal phosphateUniRule annotation1
Binding sitei203Pyridoxal phosphateUniRule annotation1
Binding sitei228Pyridoxal phosphateUniRule annotation1
Binding sitei235Pyridoxal phosphateUniRule annotation1
Binding sitei262Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei362Pyridoxal phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, One-carbon metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-1404-MONOMER
UniPathwayiUPA00193
UPA00288; UER01023

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotation
Ordered Locus Names:CBU_1419
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001135691 – 419Serine hydroxymethyltransferaseAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei229N6-(pyridoxal phosphate)lysineUniRule annotation1

Proteomic databases

PRIDEiQ83BT3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_1419

Structurei

3D structure databases

ProteinModelPortaliQ83BT3
SMRiQ83BT3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni354 – 356Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C65 Bacteria
COG0112 LUCA
HOGENOMiHOG000239404
KOiK00600
OMAiPLEHIIA

Family and domain databases

CDDicd00378 SHMT, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00051 SHMT, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR001085 Ser_HO-MeTrfase
IPR019798 Ser_HO-MeTrfase_PLP_BS
PIRSFiPIRSF000412 SHMT, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00096 SHMT, 1 hit

Sequencei

Sequence statusi: Complete.

Q83BT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEPTLTVES FDSELAGAIR DERRRQEHHV ELIASENYVS PRVLELQGSV
60 70 80 90 100
LTNKYAEGYP GRRYYGGCEF VDIAEQLAID RAKELFGADY ANVQPHSGSQ
110 120 130 140 150
ANAEAYMALM NPGDTLLAMD LSHGGHLTHG SPVSFSGKFY KAVHYGLNAH
160 170 180 190 200
GDIDYEQAAQ LAQEHKPKVI LAGFSAFSGI VDWQRFREIA DSVNAYFMTD
210 220 230 240 250
IAHVAGLVAA GVYPSPVQIA DVTTTTTHKT LRGPRAGLIL AKANPELEKR
260 270 280 290 300
LNSAVFPGSQ GGPLMHIIAA KAVAFKEAMQ PEFKTYAQQI LKNAKAMAEV
310 320 330 340 350
MKERGYTIVS GGTQNHLFLV SLLDKNISGK EAEAALGRAN ITVNKNTVPG
360 370 380 390 400
ETRSPFVTSG LRIGTPAITT RGFKEKEASQ LAHWVCDILD DIHNEKVIAD
410
VKQKAHELCG KFPVYQELD
Length:419
Mass (Da):45,801
Last modified:June 1, 2003 - v1
Checksum:i7FC39772026401DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO90917.1
RefSeqiNP_820403.1, NC_002971.3
WP_010958216.1, NC_002971.4

Genome annotation databases

EnsemblBacteriaiAAO90917; AAO90917; CBU_1419
GeneIDi1209325
31483214
KEGGicbu:CBU_1419
PATRICifig|227377.7.peg.1419

Similar proteinsi

Entry informationi

Entry nameiGLYA_COXBU
AccessioniPrimary (citable) accession number: Q83BT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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