Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein HldE

Gene

hldE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE), Bifunctional protein HldE (CBU_0678)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE), Bifunctional protein HldE (CBU_0678)
  4. no protein annotated in this organism
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei266UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 200ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437
UPA00356; UER00439

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Synonyms:rfaE
Ordered Locus Names:CBU_1655
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801061 – 475Bifunctional protein HldEAdd BLAST475

Proteomic databases

PRIDEiQ83B60

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_1655

Structurei

3D structure databases

ProteinModelPortaliQ83B60
SMRiQ83B60
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 321RibokinaseAdd BLAST321
Regioni346 – 475CytidylyltransferaseAdd BLAST130

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0 Bacteria
COG2870 LUCA
HOGENOMiHOG000237584
KOiK03272
OMAiCDPKGKD

Family and domain databases

CDDicd01172 RfaE_like, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_01603 HldE, 1 hit
InterProiView protein in InterPro
IPR023030 Bifunc_HldE
IPR002173 Carboh/pur_kinase_PfkB_CS
IPR004821 Cyt_trans-like
IPR011611 PfkB_dom
IPR011913 RfaE_dom_I
IPR011914 RfaE_dom_II
IPR029056 Ribokinase-like
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
PF00294 PfkB, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit
TIGR02198 rfaE_dom_I, 1 hit
TIGR02199 rfaE_dom_II, 1 hit
PROSITEiView protein in PROSITE
PS00583 PFKB_KINASES_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q83B60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKIDISLY EKARIVVYGD IMLDRYWYGQ ASRISPEAPI PVVNVDQIEA
60 70 80 90 100
RPGGAANVAL NVAALGASVE LMGVVGDDQE SRELETLLNK KSINCHFHRL
110 120 130 140 150
NDHPTVTKLR VLGQNQQLLR LDFEKTLKHY EDKGLLQRYQ HSLSHAQAVI
160 170 180 190 200
LSDYAKGALF NVTAPIQRAR EKGLPVLVDP KSVDFSRYAG ATLLTPNLKE
210 220 230 240 250
FEAVVGHCRT DQELEFKARA LIHQYRFEAI LITRGKQGMM LIQKEGAAIN
260 270 280 290 300
LVAHAREVYD VTGAGDTVIA VMAASLAAGG DFYEAAQLAN LAAGLVVRKL
310 320 330 340 350
GAATVTVPEL RRALHQITAS HHGILSEKAL LLAVADARAH GETIVMTNGC
360 370 380 390 400
FDILHAGHVH YLEAAKAMGH RLIVAVNDDN SVRRLKGKDR PINSLQARME
410 420 430 440 450
VLTALRAIDW VVPFSEDTPA RLITEVLPNI LVKGGDYQPS QIAGGDEVVK
460 470
NGGKVLTIPI KEGFSTSRLV EKMLN
Length:475
Mass (Da):51,922
Last modified:June 1, 2003 - v1
Checksum:i1C4AC52C4A818130
GO

Sequence cautioni

The sequence AAO91151 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO91151.2 Different initiation.
RefSeqiNP_820637.2, NC_002971.3

Genome annotation databases

EnsemblBacteriaiAAO91151; AAO91151; CBU_1655
GeneIDi1209566
KEGGicbu:CBU_1655
PATRICifig|227377.7.peg.1646

Similar proteinsi

Entry informationi

Entry nameiHLDE_COXBU
AccessioniPrimary (citable) accession number: Q83B60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: July 5, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health