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Protein

Thymidylate synthase

Gene

thyA

Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22dUMPUniRule annotation1
Active sitei197NucleophileUniRule annotation1
Binding sitei2205,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei228dUMPUniRule annotation1
Binding sitei3145,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi217 – 220dUMPUniRule annotation4
Nucleotide bindingi258 – 260dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciEFAE226185:G1G0C-1562-MONOMER
UniPathwayi
UPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:EF_1576
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000001415 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795144

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409571 – 315Thymidylate synthaseAdd BLAST315

Proteomic databases

PRIDEiQ834R3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi226185.EF1576

Chemistry databases

BindingDBiQ834R3

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ834R3
SMRiQ834R3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q834R3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEAYLALGK KILEEGHFKE DRTGTGTYSL FGYQMRFDLA KGFPLLTTKR
60 70 80 90 100
VPFGLIKSEL LWFLKGDTNI RYLLERNNHI WDEWAFERYV KSADYQGPDM
110 120 130 140 150
TDFGHRVLQD PAFAEQYKEE HQKFCDAILN DAEFAEKYGE LGNIYGAQWR
160 170 180 190 200
HWETKDGSFI DQLANVIEMI KTNPDSRRLI VSAWNPEDVP SMALPPCHTM
210 220 230 240 250
FQFYVNEGKL SCQLYQRSAD VFLGVPFNIA SYALLTHLIA HETGLEVGEF
260 270 280 290 300
VHTLGDAHLY QNHVEQMQEQ LSREVRSFPT LVLNPDKASV FDFDMEDIKV
310
EGYDPHPTIK APIAV
Length:315
Mass (Da):36,344
Last modified:June 1, 2003 - v1
Checksum:i0593A11D77D2D0A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO81363.1
RefSeqiNP_815293.1, NC_004668.1
WP_002357569.1, NZ_KE136528.1

Genome annotation databases

EnsemblBacteriaiAAO81363; AAO81363; EF_1576
GeneIDi1200476
KEGGiefa:EF1576
PATRICifig|226185.45.peg.1929

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016830 Genomic DNA Translation: AAO81363.1
RefSeqiNP_815293.1, NC_004668.1
WP_002357569.1, NZ_KE136528.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UWLX-ray2.07A/B/C/D1-315[»]
4O7UX-ray2.40A/B/C/D1-315[»]
5J7WX-ray2.50A/B/C/D1-315[»]
ProteinModelPortaliQ834R3
SMRiQ834R3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226185.EF1576

Chemistry databases

BindingDBiQ834R3
ChEMBLiCHEMBL1795144

Proteomic databases

PRIDEiQ834R3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO81363; AAO81363; EF_1576
GeneIDi1200476
KEGGiefa:EF1576
PATRICifig|226185.45.peg.1929

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
KOiK00560
OMAiKQYLDLC

Enzyme and pathway databases

UniPathwayi
UPA00575

BioCyciEFAE226185:G1G0C-1562-MONOMER

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_ENTFA
AccessioniPrimary (citable) accession number: Q834R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: October 10, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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