UniProtKB - Q82D15 (IXTPA_STRAW)
Protein
dITP/XTP pyrophosphatase
Gene
SAV_5172
Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Functioni
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation
Catalytic activityi
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 72 | Proton acceptorUniRule annotation | 1 | |
Metal bindingi | 72 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 73 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 178 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- nucleoside-triphosphatase activity Source: InterPro
- nucleoside-triphosphate diphosphatase activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleotide metabolic process Source: UniProtKB-KW
- purine nucleoside triphosphate catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | SAVE227882:G1G23-5440-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)Alternative name(s): Non-canonical purine NTP pyrophosphataseUniRule annotation Non-standard purine NTP pyrophosphataseUniRule annotation Nucleoside-triphosphate diphosphataseUniRule annotation Nucleoside-triphosphate pyrophosphataseUniRule annotation Short name: NTPaseUniRule annotation |
Gene namesi | Ordered Locus Names:SAV_5172 |
Organismi | Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) |
Taxonomic identifieri | 227882 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178237 | 1 – 200 | dITP/XTP pyrophosphataseAdd BLAST | 200 |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 227882.SAV_5172 |
Structurei
3D structure databases
ProteinModelPortali | Q82D15 |
SMRi | Q82D15 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 8 – 13 | Substrate bindingUniRule annotation | 6 | |
Regioni | 155 – 158 | Substrate bindingUniRule annotation | 4 | |
Regioni | 183 – 184 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the HAM1 NTPase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4108V82 Bacteria COG0127 LUCA |
HOGENOMi | HOG000293319 |
KOi | K02428 |
OMAi | YDPIFQP |
OrthoDBi | 1824064at2 |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_01405 Non_canon_purine_NTPase, 1 hit |
InterProi | View protein in InterPro IPR020922 dITP/XTP_pyrophosphatase IPR002637 Ham1p-like IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
i Sequence
Sequence statusi: Complete.
Q82D15-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRLILATRN AGKITELRAI LADAGLTHDL VGADAYPDIP DVKETGVTFA
60 70 80 90 100
ENALLKAHAL ARATGLPAVA DDSGLCVDVL GGAPGIFSAR WSGRHGDDRA
110 120 130 140 150
NLDLLLAQLS DISEAHRGAH FACAAALALP DGTERVVEGQ LRGTLRHTPT
160 170 180 190 200
GTNGFGYDPV LQPEGETRTC AELSAEEKNA ISHRGKAFRE LVPVVRELLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000030 Genomic DNA Translation: BAC72884.1 |
RefSeqi | WP_010986576.1, NZ_JZJK01000072.1 |
Genome annotation databases
EnsemblBacteriai | BAC72884; BAC72884; SAVERM_5172 |
KEGGi | sma:SAVERM_5172 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000030 Genomic DNA Translation: BAC72884.1 |
RefSeqi | WP_010986576.1, NZ_JZJK01000072.1 |
3D structure databases
ProteinModelPortali | Q82D15 |
SMRi | Q82D15 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
STRINGi | 227882.SAV_5172 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
EnsemblBacteriai | BAC72884; BAC72884; SAVERM_5172 |
KEGGi | sma:SAVERM_5172 |
Phylogenomic databases
eggNOGi | ENOG4108V82 Bacteria COG0127 LUCA |
HOGENOMi | HOG000293319 |
KOi | K02428 |
OMAi | YDPIFQP |
OrthoDBi | 1824064at2 |
Enzyme and pathway databases
BioCyci | SAVE227882:G1G23-5440-MONOMER |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_01405 Non_canon_purine_NTPase, 1 hit |
InterProi | View protein in InterPro IPR020922 dITP/XTP_pyrophosphatase IPR002637 Ham1p-like IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | IXTPA_STRAW | |
Accessioni | Q82D15Primary (citable) accession number: Q82D15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | June 1, 2003 | |
Last modified: | January 16, 2019 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families