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UniProtKB - Q826W3 (MEGL_STRAW)
Protein
L-methionine gamma-lyase
Gene
mgl
Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Functioni
Catalyzes the alpha,gamma-elimination of L-methionine to produce methanethiol, 2-oxobutanoate and ammonia. Is probably involved in L-methionine catabolism. Is also able to catalyze the alpha,gamma-elimination of L-homocysteine, and, to a lesser extent, the alpha,beta-elimination of L-cysteine.
1 PublicationCatalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Kineticsi
kcat is 2.90 sec(-1) for the alpha,gamma-elimination of L-methionine. kcat is 1.67 sec(-1) for the alpha,gamma-elimination of L-homocysteine. kcat is 0.03 sec(-1) for the alpha,beta-elimination of L-cysteine.1 Publication
- KM=1.60 mM for L-methionine1 Publication
- KM=1.78 mM for L-homocysteine1 Publication
- KM=0.20 mM for L-cysteine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 131 | SubstrateBy similarity | 1 | |
Binding sitei | 365 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- homocysteine desulfhydrase activity Source: UniProtKB-EC
- L-cysteine desulfhydrase activity Source: RHEA
- methionine gamma-lyase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- transsulfuration Source: InterPro
Keywordsi
Molecular function | Lyase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 4.4.1.11, 5980 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mglImported ORF Names:SAVERM_7062Imported |
Organismi | Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) |
Taxonomic identifieri | 227882 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000443073 | 1 – 413 | L-methionine gamma-lyaseAdd BLAST | 413 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 221 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Interactioni
Subunit structurei
Homotetramer; dimer of active dimers.
By similarityProtein-protein interaction databases
STRINGi | 227882.SAV_7062 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 77 | Pyridoxal phosphate binding; shared with dimeric partnerBy similarity | 3 | |
Regioni | 105 – 106 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 218 – 220 | Pyridoxal phosphate bindingBy similarity | 3 | |
Regioni | 388 – 413 | DisorderedSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the trans-sulfuration enzymes family.Curated
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_0_11 |
OMAi | EVTWTDP |
OrthoDBi | 637281at2 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
Q826W3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDDGRGAGGF GGGPAVRALD TEAVHAGRDD LARQGLHAAP IDLSTTYPSY
60 70 80 90 100
DSRAEAARID AFAADGAEPA GPPVYGRLGN PTVARFETAL ARLEGTDSAV
110 120 130 140 150
AFASGMAALS AVLLVRNAMG LRHVVAVRPL YGCSDHLLTA GLLGSEVTWV
160 170 180 190 200
DPAGVADALR PDTGLVMVES PANPTLAELD LRALAHACGS VPLLADNTFA
210 220 230 240 250
TPVLQRPAEH GARLVLHSAT KYLGGHGDVM AGVVACDEEF ARGLRQIRFA
260 270 280 290 300
TGGVLHPLAG YLLLRGLSTL PIRVRAASSN AAELARRLAA DPRVARVHYP
310 320 330 340 350
RIGGAMIAFE VYGDPHEVIA GVRLITPAVS LGSVDSLIQH PASISHRIVD
360 370 380 390 400
AADRRGAGVS DRLLRLSVGL EDVEDLWADL DGALGTDRLP ETAGAGREPS
410
RTALRLPERA ADR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000030 Genomic DNA Translation: BAC74773.1 |
RefSeqi | WP_010988457.1, NZ_JZJK01000085.1 |
Genome annotation databases
EnsemblBacteriai | BAC74773; BAC74773; SAVERM_7062 |
KEGGi | sma:SAVERM_7062 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000030 Genomic DNA Translation: BAC74773.1 |
RefSeqi | WP_010988457.1, NZ_JZJK01000085.1 |
3D structure databases
AlphaFoldDBi | Q826W3 |
SMRi | Q826W3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 227882.SAV_7062 |
Genome annotation databases
EnsemblBacteriai | BAC74773; BAC74773; SAVERM_7062 |
KEGGi | sma:SAVERM_7062 |
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_0_11 |
OMAi | EVTWTDP |
OrthoDBi | 637281at2 |
Enzyme and pathway databases
BRENDAi | 4.4.1.11, 5980 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MEGL_STRAW | |
Accessioni | Q826W3Primary (citable) accession number: Q826W3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2018 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families