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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14SubstrateUniRule annotation1
Binding sitei67SubstrateUniRule annotation1
Active sitei76Proton donorUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Sitei168Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei199SubstrateUniRule annotation1
Sitei217Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei226Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:BA_5170, GBAA_5170, BAS4806
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000118371 – 288Diaminopimelate epimeraseAdd BLAST288

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ81XR2, 3 interactors
STRINGi260799.BAS4806

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 11Combined sources11
Beta strandi14 – 20Combined sources7
Turni21 – 23Combined sources3
Helixi28 – 30Combined sources3
Helixi31 – 39Combined sources9
Turni41 – 43Combined sources3
Beta strandi48 – 54Combined sources7
Beta strandi57 – 67Combined sources11
Turni76 – 79Combined sources4
Helixi80 – 89Combined sources10
Beta strandi94 – 102Combined sources9
Beta strandi105 – 126Combined sources22
Helixi132 – 134Combined sources3
Beta strandi143 – 152Combined sources10
Beta strandi155 – 172Combined sources18
Helixi176 – 178Combined sources3
Turni181 – 183Combined sources3
Helixi184 – 189Combined sources6
Beta strandi199 – 207Combined sources9
Beta strandi210 – 216Combined sources7
Beta strandi218 – 220Combined sources3
Helixi227 – 239Combined sources13
Beta strandi249 – 252Combined sources4
Beta strandi257 – 262Combined sources6
Beta strandi268 – 273Combined sources6
Beta strandi275 – 286Combined sources12

3D structure databases

ProteinModelPortaliQ81XR2
SMRiQ81XR2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81XR2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 78Substrate bindingUniRule annotation2
Regioni217 – 218Substrate bindingUniRule annotation2
Regioni227 – 228Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q81XR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQFSFTKMH GLGNSYIYVN MFEEQIPEED LALVAEKVSN INTGIGADGM
60 70 80 90 100
ILICPSDVAP VKMRMFNNDG SEGKSCGNGL RCVAKYAYEH KLVEDTVFTI
110 120 130 140 150
ETLAGIVTAE VTVEEGKVTL AKIDMGAPRL TRAEIPMLGE GETPFIRENF
160 170 180 190 200
LYNNHRYAFT AVSMGNPHAV IFVDDVEQAP LTTLGPVLET HEMFPERVNV
210 220 230 240 250
EFIEILNEEE MNFRVWERGS GVTQACGTGA CAAVVASILN GKMERGKEIT
260 270 280
VHLAGGDLMI AWTEEGNVLM KGPAEVICRG VYEYKIEA
Length:288
Mass (Da):31,613
Last modified:June 1, 2003 - v1
Checksum:iF82364D2AC4202CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP28840.1
AE017225 Genomic DNA Translation: AAT57099.1
AE017334 Genomic DNA Translation: AAT70160.1
RefSeqiNP_847354.1, NC_003997.3
WP_000077386.1, NZ_QANP01000002.1
YP_031049.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP28840; AAP28840; BA_5170
AAT57099; AAT57099; BAS4806
AAT70160; AAT70160; GBAA_5170
GeneIDi1084119
2848459
KEGGiban:BA_5170
bar:GBAA_5170
bat:BAS4806
PATRICifig|198094.11.peg.5132

Similar proteinsi

Entry informationi

Entry nameiDAPF_BACAN
AccessioniPrimary (citable) accession number: Q81XR2
Secondary accession number(s): Q6F086, Q6HRI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: July 18, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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