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Protein

Probable endonuclease 4

Gene

nfo

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1UniRule annotation1
Metal bindingi111Zinc 1UniRule annotation1
Metal bindingi146Zinc 1UniRule annotation1
Metal bindingi146Zinc 2UniRule annotation1
Metal bindingi180Zinc 2UniRule annotation1
Metal bindingi183Zinc 3UniRule annotation1
Metal bindingi215Zinc 2UniRule annotation1
Metal bindingi228Zinc 3UniRule annotation1
Metal bindingi230Zinc 3UniRule annotation1
Metal bindingi260Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:BA_4508, GBAA_4508, BAS4186
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908191 – 298Probable endonuclease 4Add BLAST298

Interactioni

Protein-protein interaction databases

STRINGi260799.BAS4186

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi12 – 14Combined sources3
Helixi16 – 24Combined sources9
Turni25 – 27Combined sources3
Beta strandi29 – 33Combined sources5
Helixi46 – 49Combined sources4
Helixi51 – 60Combined sources10
Beta strandi66 – 69Combined sources4
Helixi82 – 101Combined sources20
Beta strandi102 – 104Combined sources3
Beta strandi107 – 110Combined sources4
Helixi120 – 134Combined sources15
Beta strandi140 – 146Combined sources7
Helixi159 – 168Combined sources10
Helixi172 – 174Combined sources3
Beta strandi175 – 180Combined sources6
Helixi181 – 186Combined sources6
Helixi191 – 205Combined sources15
Helixi208 – 210Combined sources3
Beta strandi211 – 216Combined sources6
Beta strandi218 – 221Combined sources4
Beta strandi235 – 238Combined sources4
Helixi240 – 247Combined sources8
Helixi250 – 252Combined sources3
Beta strandi257 – 259Combined sources3
Beta strandi267 – 269Combined sources3
Helixi275 – 284Combined sources10
Helixi291 – 296Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XP3X-ray2.57A1-298[»]
ProteinModelPortaliQ81LV1
SMRiQ81LV1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ81LV1

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU Bacteria
COG0648 LUCA
HOGENOMiHOG000224895
KOiK01151
OMAiHPGSHLK

Family and domain databases

CDDicd00019 AP2Ec, 1 hit
HAMAPiMF_00152 Nfo, 1 hit
InterProiView protein in InterPro
IPR001719 AP_endonuc_2
IPR018246 AP_endonuc_F2_Zn_BS
IPR036237 Xyl_isomerase-like_sf
IPR013022 Xyl_isomerase-like_TIM-brl
PANTHERiPTHR21445 PTHR21445, 1 hit
PfamiView protein in Pfam
PF01261 AP_endonuc_2, 1 hit
SMARTiView protein in SMART
SM00518 AP2Ec, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR00587 nfo, 1 hit
PROSITEiView protein in PROSITE
PS00729 AP_NUCLEASE_F2_1, 1 hit
PS00730 AP_NUCLEASE_F2_2, 1 hit
PS00731 AP_NUCLEASE_F2_3, 1 hit
PS51432 AP_NUCLEASE_F2_4, 1 hit

Sequencei

Sequence statusi: Complete.

Q81LV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIGSHVSM SGKKMLLAAS EEAVSYGATT FMIYTGAPQN TRRKPIEELN
60 70 80 90 100
IEAGRKHMEQ NGIEEIIVHA PYIINVGNTT KPETFQLGVD FLRMEIERTS
110 120 130 140 150
ALGVAKQIVL HPGAHVGAGA DAGIQQIIKG LNEVLTPDQT VNIALETMAG
160 170 180 190 200
KGTECGRSFE EIAKIIDGVK YNEKLSVCFD TCHTHDAGYD IVNNFDGVLN
210 220 230 240 250
EFDKIVGIDR LQVLHINDSK NVRGAGKDRH ENIGFGHIGY KALHHIVHHP
260 270 280 290
QLTHVPKILE TPYVGEDKKD KKPPYKLEIE MLKNGTFDEG LLEKIKAQ
Length:298
Mass (Da):32,909
Last modified:June 1, 2003 - v1
Checksum:iC5178AB085784C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP28219.1
AE017334 Genomic DNA Translation: AAT33627.1
AE017225 Genomic DNA Translation: AAT56486.1
RefSeqiNP_846733.1, NC_003997.3
WP_000912468.1, NZ_PPES01000001.1
YP_030435.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP28219; AAP28219; BA_4508
AAT33627; AAT33627; GBAA_4508
AAT56486; AAT56486; BAS4186
GeneIDi1088232
2852108
KEGGiban:BA_4508
bar:GBAA_4508
bat:BAS4186
PATRICifig|198094.11.peg.4476

Similar proteinsi

Entry informationi

Entry nameiEND4_BACAN
AccessioniPrimary (citable) accession number: Q81LV1
Secondary accession number(s): Q6HTA3, Q6KMJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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