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Protein

DNA polymerase IV

Gene

dinB

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19MagnesiumUniRule annotation1
Sitei24Substrate discriminationUniRule annotation1
Metal bindingi115MagnesiumUniRule annotation1
Active sitei116UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:BC_4142
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739001 – 415DNA polymerase IVAdd BLAST415

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226900.BC4142

Structurei

3D structure databases

ProteinModelPortaliQ818U9
SMRiQ818U9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 196UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0389 LUCA
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q818U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTMREMYPK NGRVILHVDM NCFFASVEIA HDPSLQGKPL AVAGNEKERK
60 70 80 90 100
GIIITCSYEA REYGIRTTMP LWEAKRLCPQ LVVRRPNFTL YREASFQMFQ
110 120 130 140 150
VLSRFTEKIQ PVSIDEGYLD ITDCYALGSP LEIAKMIQQA LLTELQLPCS
160 170 180 190 200
IGIAPNLFLA KTASDMKKPL GITVLRKRDI PEMIWPLSVE AMHGIGEKTA
210 220 230 240 250
EKLNEIHIHT IEQLAKGDEH IIRAKIGKHG IDLQKRAKGT DDREVDPSQM
260 270 280 290 300
GQHKSVGNSM TFSKDMDEEK ELLDMLERLS KSVSKRLQKR TLVSYNIQIM
310 320 330 340 350
IKYHDRRTVT RSKQLKNAIW EERDIFQAAS RLWKQHWDGD SVRLLGVTAT
360 370 380 390 400
EIEWKTESVK QLDLFSFEED AKKEPLLAVI DQINDKYGTP ILQRGSQLLR
410
KQEKSFQQKL ENKFM
Length:415
Mass (Da):47,776
Last modified:June 1, 2003 - v1
Checksum:i423C3352353B3CFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA Translation: AAP11058.1

Genome annotation databases

EnsemblBacteriaiAAP11058; AAP11058; BC_4142

Similar proteinsi

Entry informationi

Entry nameiDPO4_BACCR
AccessioniPrimary (citable) accession number: Q818U9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health