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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B

Gene

Mgat4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-(beta-D-GlcNAc-(1->4))-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975577 N-Glycan antennae elongation
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT54 Glycosyltransferase Family 54

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
Short name:
GlcNAc-T IVb
Short name:
GnT-IVb
Short name:
N-acetylglucosaminyltransferase IVb
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb
Gene namesi
Name:Mgat4b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2143974 Mgat4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 548LumenalSequence analysisAdd BLAST520

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885941 – 548Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase BAdd BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi87N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ812F8
PaxDbiQ812F8
PeptideAtlasiQ812F8
PRIDEiQ812F8

PTM databases

iPTMnetiQ812F8
PhosphoSitePlusiQ812F8

Expressioni

Gene expression databases

BgeeiENSMUSG00000036620 Expressed in 283 organ(s), highest expression level in brown adipose tissue
ExpressionAtlasiQ812F8 baseline and differential
GenevisibleiQ812F8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043346

Structurei

3D structure databases

ProteinModelPortaliQ812F8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 83Sequence analysisAdd BLAST48

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC Eukaryota
ENOG410XPV0 LUCA
GeneTreeiENSGT00550000074578
HOGENOMiHOG000015085
HOVERGENiHBG053194
InParanoidiQ812F8
KOiK00738
OMAiLKLWNIT
OrthoDBiEOG091G06KB
PhylomeDBiQ812F8
TreeFamiTF324570

Family and domain databases

InterProiView protein in InterPro
IPR006759 Glyco_transf_54
PANTHERiPTHR12062 PTHR12062, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q812F8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLRNGTFLT LLLFCLCAFL SLSWYAALSG QKGDVVDIYQ REFLALRDRL
60 70 80 90 100
HAAEQESLKR SKELNLVLEE IKRAVSERQA LRDGEGNRTW GRLTEDPRLK
110 120 130 140 150
PWNVSHRHVL HLPTVFHHLP HLLAKESSLQ PAVRVGQGRT GVSVVMGIPS
160 170 180 190 200
VRREVHSYLT DTLHSLISEL SPQEKEDSVI VVLIAETDPQ YTSAVTENIK
210 220 230 240 250
ALFPTEIHSG LLEVISPSPH FYPDFSRLRE SFGDPKERVR WRTKQNLDYC
260 270 280 290 300
FLMMYAQSKG IYYVQLEDDI VAKPNYLSTM KNFALQQPSE DWMILEFSQL
310 320 330 340 350
GFIGKMFKSL DLSLIVEFIL MFYRDKPIDW LLDHILWVKV CNPEKDAKHC
360 370 380 390 400
DRQKANLRIR FKPSLFQHVG THSSLAGKIQ KLKDKDFGKH ALRKEHVNPP
410 420 430 440 450
AEVSTSLKTY QHFTLEKAYL REDFFWAFTP AAGDFIRFRF FQPLRLERFF
460 470 480 490 500
FRSGNIEHPE DKLFNTSVEV LPFDNPQSEK EALQEGRSAT LRYPRSPDGY
510 520 530 540
LQIGSFYKGV AEGEVDPAFG PLEALRLSIQ TDSPVWVILS EIFLKKAD
Length:548
Mass (Da):63,302
Last modified:June 1, 2003 - v1
Checksum:iA66F88F4A05FBF27
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8Y5H5A8Y5H5_MOUSE
Alpha-1,3-mannosyl-glycoprotein 4-b...
Mgat4b
148Annotation score:
F7BME0F7BME0_MOUSE
Alpha-1,3-mannosyl-glycoprotein 4-b...
Mgat4b
252Annotation score:

Sequence cautioni

The sequence AAH26638 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH31613 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti395 – 396EH → SY in BAC55019 (PubMed:10372966).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053218 mRNA Translation: BAC55019.1
AB053219 Genomic DNA Translation: BAC55020.1
AL627187 Genomic DNA Translation: CAI25119.1
AK155336 mRNA Translation: BAE33202.1
BC026638 mRNA Translation: AAH26638.1 Different initiation.
BC031613 mRNA Translation: AAH31613.1 Different initiation.
CCDSiCCDS24630.1
RefSeqiNP_666038.3, NM_145926.3
UniGeneiMm.86759

Genome annotation databases

EnsembliENSMUST00000041725; ENSMUSP00000043346; ENSMUSG00000036620
GeneIDi103534
KEGGimmu:103534
UCSCiuc007iry.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053218 mRNA Translation: BAC55019.1
AB053219 Genomic DNA Translation: BAC55020.1
AL627187 Genomic DNA Translation: CAI25119.1
AK155336 mRNA Translation: BAE33202.1
BC026638 mRNA Translation: AAH26638.1 Different initiation.
BC031613 mRNA Translation: AAH31613.1 Different initiation.
CCDSiCCDS24630.1
RefSeqiNP_666038.3, NM_145926.3
UniGeneiMm.86759

3D structure databases

ProteinModelPortaliQ812F8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043346

Protein family/group databases

CAZyiGT54 Glycosyltransferase Family 54

PTM databases

iPTMnetiQ812F8
PhosphoSitePlusiQ812F8

Proteomic databases

MaxQBiQ812F8
PaxDbiQ812F8
PeptideAtlasiQ812F8
PRIDEiQ812F8

Protocols and materials databases

DNASUi103534
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041725; ENSMUSP00000043346; ENSMUSG00000036620
GeneIDi103534
KEGGimmu:103534
UCSCiuc007iry.2 mouse

Organism-specific databases

CTDi11282
MGIiMGI:2143974 Mgat4b

Phylogenomic databases

eggNOGiENOG410IEDC Eukaryota
ENOG410XPV0 LUCA
GeneTreeiENSGT00550000074578
HOGENOMiHOG000015085
HOVERGENiHBG053194
InParanoidiQ812F8
KOiK00738
OMAiLKLWNIT
OrthoDBiEOG091G06KB
PhylomeDBiQ812F8
TreeFamiTF324570

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-975577 N-Glycan antennae elongation

Miscellaneous databases

ChiTaRSiMgat4b mouse
PROiPR:Q812F8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036620 Expressed in 283 organ(s), highest expression level in brown adipose tissue
ExpressionAtlasiQ812F8 baseline and differential
GenevisibleiQ812F8 MM

Family and domain databases

InterProiView protein in InterPro
IPR006759 Glyco_transf_54
PANTHERiPTHR12062 PTHR12062, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMGT4B_MOUSE
AccessioniPrimary (citable) accession number: Q812F8
Secondary accession number(s): Q3U2D9, Q812F9, Q8R0L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: September 12, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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