UniProtKB - Q811U4 (MFN1_MOUSE)
Mitofusin-1
Mfn1
Functioni
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 284 | GTPBy similarity | 1 | |
Binding sitei | 286 | GTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 85 – 90 | GTPBy similarity | 6 | |
Nucleotide bindingi | 237 – 240 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- identical protein binding Source: MGI
GO - Biological processi
- GTP metabolic process Source: UniProtKB
- intracellular distribution of mitochondria Source: MGI
- mitochondrial fusion Source: UniProtKB
- mitochondrial membrane fusion Source: UniProtKB
- mitochondrion localization Source: UniProtKB
- mitochondrion morphogenesis Source: MGI
- negative regulation of mitochondrial fission Source: MGI
- positive regulation of dendritic spine morphogenesis Source: MGI
- positive regulation of mitochondrial fusion Source: MGI
- positive regulation of mitochondrial membrane potential Source: CACAO
Keywordsi
Molecular function | Developmental protein, Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5205685, PINK1-PRKN Mediated Mitophagy R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Mfn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914664, Mfn1 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 1 Publication; Multi-pass membrane protein Curated
Mitochondrion
- integral component of mitochondrial outer membrane Source: UniProtKB
- intrinsic component of mitochondrial outer membrane Source: GO_Central
- mitochondrial inner membrane Source: MGI
- mitochondrial outer membrane Source: MGI
- mitochondrion Source: MGI
- outer mitochondrial membrane protein complex Source: UniProtKB
Other locations
- integral component of membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 584 | CytoplasmicSequence analysisAdd BLAST | 584 | |
Transmembranei | 585 – 605 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 606 – 608 | Mitochondrial intermembraneSequence analysis | 3 | |
Transmembranei | 609 – 629 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 630 – 741 | CytoplasmicSequence analysisAdd BLAST | 112 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127673 | 1 – 741 | Mitofusin-1Add BLAST | 741 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | Q811U4 |
jPOSTi | Q811U4 |
MaxQBi | Q811U4 |
PaxDbi | Q811U4 |
PeptideAtlasi | Q811U4 |
PRIDEi | Q811U4 |
ProteomicsDBi | 292227 |
PTM databases
iPTMneti | Q811U4 |
PhosphoSitePlusi | Q811U4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000027668, Expressed in brain and 308 other tissues |
Genevisiblei | Q811U4, MM |
Interactioni
Subunit structurei
Homodimer, also in the absence of bound GTP (By similarity). Forms higher oligomers in the presence of a transition state GTP analog (By similarity). Forms homomultimers and heteromultimers with MFN2 (PubMed:12527753). Oligomerization is essential for mitochondrion fusion (PubMed:15297672).
Component of a high molecular weight multiprotein complex (By similarity).
Interacts with VAT1 (By similarity).
Interacts with THG1L; THG1L probably functions as a guanyl-nucleotide exchange factor/GEF, activating MFN1.
By similarity2 PublicationsBinary interactionsi
Q811U4
With | #Exp. | IntAct |
---|---|---|
LRRK2 [Q5S007] from Homo sapiens. | 3 | EBI-9029118,EBI-5323863 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 212170, 4 interactors |
DIPi | DIP-60969N |
IntActi | Q811U4, 4 interactors |
STRINGi | 10090.ENSMUSP00000088801 |
Miscellaneous databases
RNActi | Q811U4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q811U4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q811U4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 321 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 250 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 73 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 65 | |
Regioni | 82 – 89 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 108 – 109 | G2 motifPROSITE-ProRule annotation | 2 | |
Regioni | 178 – 181 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 237 – 240 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 266 | G5 motifPROSITE-ProRule annotation | 1 | |
Regioni | 338 – 364 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 27 | |
Regioni | 703 – 734 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 371 – 408 | 1 PublicationAdd BLAST | 38 | |
Coiled coili | 677 – 735 | 1 PublicationAdd BLAST | 59 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0448, Eukaryota |
GeneTreei | ENSGT00390000013727 |
HOGENOMi | CLU_021212_1_0_1 |
InParanoidi | Q811U4 |
OMAi | FQEDIMF |
OrthoDBi | 216494at2759 |
TreeFami | TF314289 |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027088, Mitofusin-1 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF2, PTHR10465:SF2, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAETVSPLKH FVLAKKAITA IFGQLLEFVT EGSHFVEATY RNPELDRIAS
60 70 80 90 100
EDDLVEIQGY RNKLAVIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV
110 120 130 140 150
LPSGIGHTTN CFLSVEGTDG DKAYLMTEGS DEKKSVKTVN QLAHALHMDK
160 170 180 190 200
DLKAGCLVHV FWPKAKCALL RDDLVLVDSP GTDVTTELDI WIDKFCLDAD
210 220 230 240 250
VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD ASASEPEYME
260 270 280 290 300
DVRRQHMERC LHFLVEELKV VSPSEARNRI FFVSAKEVLN SRKHKAQGMP
310 320 330 340 350
EGGGALAEGF QARLQEFQNF EQTFEECISQ SAVKTKFEQH TIRAKQILDT
360 370 380 390 400
VKNILDSVNV AAAEKRVYSM EEREDQIDRL DFIRNQMNLL TLDVKKKIKE
410 420 430 440 450
VTEEVANKVS CAMTDEICRL SVLVDEFCSE FHPTPSVLKV YKSELNKHIE
460 470 480 490 500
DGMGRNLADR CTNEVNASIL QSQQEIIENL KPLLPAGIQN KLHTLIPCKK
510 520 530 540 550
FDLSYDLNCH KLCSDFQEDI VFRFSLGWSS LVHRFLGSTN AQRVLLGLSE
560 570 580 590 600
PIFQVPRSLA STPTAPSNPA APDNAAQEEL MITLITGLAS LTSRTSMGII
610 620 630 640 650
VVGGVIWKTV GWKLISVTLS MYGALYLYER LTWTTRAKER AFKQQFVNYA
660 670 680 690 700
TEKLQMIVSF TSANCSHQVQ QEMATTFARL CQQVDVTQKH LEEEIARLSK
710 720 730 740
EIDQLEKIQN NSKLLRNKAV QLESELENFS KQFLHPSSGE S
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z5P8 | D3Z5P8_MOUSE | Mitofusin-1 | Mfn1 | 239 | Annotation score: | ||
D3Z4L5 | D3Z4L5_MOUSE | Mitofusin-1 | Mfn1 | 189 | Annotation score: | ||
F6XRI9 | F6XRI9_MOUSE | Mitofusin-1 | Mfn1 | 319 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 | E → G in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 328 – 329 | IS → VL in BAB31111 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 335 | T → A in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 521 | V → A in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 588 | L → W in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 619 | L → S in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 718 | K → R in BAB31111 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 720 | V → I in AAH02133 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 720 | V → I in AAH56641 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY174062 mRNA Translation: AAO34660.1 AK009490 mRNA Translation: BAC25260.1 Different initiation. AK018181 mRNA Translation: BAB31111.1 AK141611 mRNA Translation: BAE24764.1 BC002133 mRNA Translation: AAH02133.1 BC047050 mRNA Translation: AAH47050.1 Different initiation. BC056641 mRNA Translation: AAH56641.1 |
CCDSi | CCDS17296.1 |
RefSeqi | NP_077162.2, NM_024200.4 |
Genome annotation databases
Ensembli | ENSMUST00000091257; ENSMUSP00000088801; ENSMUSG00000027668 ENSMUST00000118286; ENSMUSP00000113251; ENSMUSG00000027668 |
GeneIDi | 67414 |
KEGGi | mmu:67414 |
UCSCi | uc008owi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY174062 mRNA Translation: AAO34660.1 AK009490 mRNA Translation: BAC25260.1 Different initiation. AK018181 mRNA Translation: BAB31111.1 AK141611 mRNA Translation: BAE24764.1 BC002133 mRNA Translation: AAH02133.1 BC047050 mRNA Translation: AAH47050.1 Different initiation. BC056641 mRNA Translation: AAH56641.1 |
CCDSi | CCDS17296.1 |
RefSeqi | NP_077162.2, NM_024200.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T3J | X-ray | 2.50 | A | 660-735 | [»] | |
SMRi | Q811U4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 212170, 4 interactors |
DIPi | DIP-60969N |
IntActi | Q811U4, 4 interactors |
STRINGi | 10090.ENSMUSP00000088801 |
PTM databases
iPTMneti | Q811U4 |
PhosphoSitePlusi | Q811U4 |
Proteomic databases
EPDi | Q811U4 |
jPOSTi | Q811U4 |
MaxQBi | Q811U4 |
PaxDbi | Q811U4 |
PeptideAtlasi | Q811U4 |
PRIDEi | Q811U4 |
ProteomicsDBi | 292227 |
Protocols and materials databases
ABCDi | Q811U4, 1 sequenced antibody |
Antibodypediai | 33743, 508 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000091257; ENSMUSP00000088801; ENSMUSG00000027668 ENSMUST00000118286; ENSMUSP00000113251; ENSMUSG00000027668 |
GeneIDi | 67414 |
KEGGi | mmu:67414 |
UCSCi | uc008owi.2, mouse |
Organism-specific databases
CTDi | 55669 |
MGIi | MGI:1914664, Mfn1 |
Phylogenomic databases
eggNOGi | KOG0448, Eukaryota |
GeneTreei | ENSGT00390000013727 |
HOGENOMi | CLU_021212_1_0_1 |
InParanoidi | Q811U4 |
OMAi | FQEDIMF |
OrthoDBi | 216494at2759 |
TreeFami | TF314289 |
Enzyme and pathway databases
Reactomei | R-MMU-5205685, PINK1-PRKN Mediated Mitophagy R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 67414, 16 hits in 52 CRISPR screens |
ChiTaRSi | Mfn1, mouse |
EvolutionaryTracei | Q811U4 |
PROi | PR:Q811U4 |
RNActi | Q811U4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027668, Expressed in brain and 308 other tissues |
Genevisiblei | Q811U4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027088, Mitofusin-1 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF2, PTHR10465:SF2, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MFN1_MOUSE | |
Accessioni | Q811U4Primary (citable) accession number: Q811U4 Secondary accession number(s): Q3URC4 Q9D395 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families