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Protein

Proline-rich membrane anchor 1

Gene

Prima1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to anchor acetylcholinesterase (ACHE) to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses in brain. Also able to organize ACHE into tetramers.1 Publication

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein membrane anchor Source: MGI

GO - Biological processi

  • establishment of localization in cell Source: MGI
  • neurotransmitter catabolic process Source: UniProtKB-KW

Keywordsi

Biological processNeurotransmitter degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich membrane anchor 1
Short name:
PRiMA
Gene namesi
Name:Prima1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1926097 Prima1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 92ExtracellularSequence analysisAdd BLAST57
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 153CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109 – 121VLVII…AIKRK → GIAIICLKAISDM: Decrease in ACHE tetramer recruitment to membrane rafts. 1 PublicationAdd BLAST13
Mutagenesisi114C → A: Does not abolish the localization of ACHE. 2 Publications1
Mutagenesisi114C → S: No effect on ACHE tetramer recruitment to membrane rafts. 2 Publications1
Mutagenesisi131S → A: No effect on ACHE tetramer recruitment to membrane rafts; when associated with 138-A--A-142. 1
Mutagenesisi138 – 142SSSQS → AAAQA: No effect on ACHE tetramer recruitment to membrane rafts; when associated with A-131. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35By similarityAdd BLAST35
ChainiPRO_000002210836 – 153Proline-rich membrane anchor 1Add BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ810F0
PRIDEiQ810F0

PTM databases

PhosphoSitePlusiQ810F0

Expressioni

Tissue specificityi

Predominantly expressed in the central nervous system, including in the brain. Also expressed in muscle, heart and kidney. Isoform 1 may be predominant in the cortex and striatum, while isoform 2 is more abundant in the cerebellum.2 Publications

Gene expression databases

CleanExiMM_PRIMA1

Interactioni

Subunit structurei

Interacts with ACHE, probably through disulfide bonds.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074017

Structurei

3D structure databases

SMRiQ810F0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 70PRADAdd BLAST15

Domaini

The proline-rich attachment domain (PRAD) binds the AChE catalytic subunits.1 Publication

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZWQ Eukaryota
ENOG410YWDZ LUCA
HOGENOMiHOG000115697
HOVERGENiHBG053680
InParanoidiQ810F0

Family and domain databases

InterProiView protein in InterPro
IPR039466 RIMA1
PANTHERiPTHR45212 PTHR45212, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q810F0-1) [UniParc]FASTAAdd to basket
Also known as: Variant I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLLRDLVPRH GCCWPSLLLH CALHPLWGLV QVTHAEPQKS CSKVTDSCQH
60 70 80 90 100
ICQCRPPPPL PPPPPPPPPP RLLSAPAPNS TSCPAEDSWW SGLVIIVAVV
110 120 130 140 150
CASLVFLTVL VIICYKAIKR KPLRKDENGT SVAEYPMSSS QSHKGVDVNA

AVV
Length:153
Mass (Da):16,564
Last modified:October 3, 2003 - v2
Checksum:iF323F7BABD5F28C9
GO
Isoform 2 (identifier: Q810F0-2) [UniParc]FASTAAdd to basket
Also known as: Variant II

The sequence of this isoform differs from the canonical sequence as follows:
     121-124: KPLR → NQAI
     125-153: Missing.

Show »
Length:124
Mass (Da):13,506
Checksum:i7313C2B8DB2C32C2
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VQJ2F8VQJ2_MOUSE
Proline-rich membrane anchor 1
Prima1
153Annotation score:
D6RE30D6RE30_MOUSE
Proline-rich membrane anchor 1
Prima1
124Annotation score:
D6RCX4D6RCX4_MOUSE
Proline-rich membrane anchor 1
Prima1
48Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008496121 – 124KPLR → NQAI in isoform 2. 1 Publication4
Alternative sequenceiVSP_008497125 – 153Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043275 mRNA Translation: AAK85717.1
AY225515 mRNA Translation: AAO74852.1
CCDSiCCDS26127.1 [Q810F0-1]
RefSeqiNP_579942.2, NM_133364.2
UniGeneiMm.217019

Genome annotation databases

GeneIDi170952
KEGGimmu:170952

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043275 mRNA Translation: AAK85717.1
AY225515 mRNA Translation: AAO74852.1
CCDSiCCDS26127.1 [Q810F0-1]
RefSeqiNP_579942.2, NM_133364.2
UniGeneiMm.217019

3D structure databases

SMRiQ810F0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074017

PTM databases

PhosphoSitePlusiQ810F0

Proteomic databases

PaxDbiQ810F0
PRIDEiQ810F0

Protocols and materials databases

DNASUi170952
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170952
KEGGimmu:170952

Organism-specific databases

CTDi145270
MGIiMGI:1926097 Prima1

Phylogenomic databases

eggNOGiENOG410IZWQ Eukaryota
ENOG410YWDZ LUCA
HOGENOMiHOG000115697
HOVERGENiHBG053680
InParanoidiQ810F0

Miscellaneous databases

PROiPR:Q810F0
SOURCEiSearch...

Gene expression databases

CleanExiMM_PRIMA1

Family and domain databases

InterProiView protein in InterPro
IPR039466 RIMA1
PANTHERiPTHR45212 PTHR45212, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRIMA_MOUSE
AccessioniPrimary (citable) accession number: Q810F0
Secondary accession number(s): Q8VHC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 7, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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