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Protein

Rabankyrin-5

Gene

Ankfy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI3P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport (By similarity). Involved in macropinocytosis; the function is dependent on Rab5-GTP.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1104 – 1164FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rabankyrin-51 Publication
Short name:
Rank-5
Alternative name(s):
Ankyrin repeat and FYVE domain-containing protein 1
Ankyrin repeats hooked to a zinc finger motif
Gene namesi
Name:Ankfy1
Synonyms:Ankhzn, Kiaa1255
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1337008 Ankfy1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000668912 – 1169Rabankyrin-5Add BLAST1168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei270PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ810B6
PaxDbiQ810B6
PeptideAtlasiQ810B6
PRIDEiQ810B6

PTM databases

iPTMnetiQ810B6
PhosphoSitePlusiQ810B6

Expressioni

Tissue specificityi

Expressed in kidney proximal tubule epithelial cells; at protein level.1 Publication

Gene expression databases

BgeeiENSMUSG00000020790 Expressed in 306 organ(s), highest expression level in tracheobronchial tree
CleanExiMM_ANKFY1
GenevisibleiQ810B6 MM

Interactioni

Subunit structurei

Interacts with RAB5A (in GTP-bound form). Interacts with RHOD (independent of GTP-loaded status). Interacts with EHD1. Interacts with VPS26A; the interaction is indepenedent of EHD1 and is indicative for an association with the cargo recognition subcomplex of the retromer complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198103, 8 interactors
IntActiQ810B6, 6 interactors
STRINGi10090.ENSMUSP00000118751

Structurei

3D structure databases

ProteinModelPortaliQ810B6
SMRiQ810B6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 130BTBPROSITE-ProRule annotationAdd BLAST63
Repeati217 – 247ANK 1Add BLAST31
Repeati255 – 284ANK 2Add BLAST30
Repeati288 – 317ANK 3Add BLAST30
Repeati322 – 362ANK 4Add BLAST41
Repeati366 – 396ANK 5Add BLAST31
Repeati490 – 519ANK 6Add BLAST30
Repeati542 – 572ANK 7Add BLAST31
Repeati588 – 617ANK 8Add BLAST30
Repeati621 – 650ANK 9Add BLAST30
Repeati654 – 683ANK 10Add BLAST30
Repeati687 – 716ANK 11Add BLAST30
Repeati724 – 753ANK 12Add BLAST30
Repeati769 – 798ANK 13Add BLAST30
Repeati802 – 832ANK 14Add BLAST31
Repeati836 – 865ANK 15Add BLAST30
Repeati870 – 899ANK 16Add BLAST30
Repeati905 – 934ANK 17Add BLAST30
Repeati938 – 967ANK 18Add BLAST30
Repeati971 – 1001ANK 19Add BLAST31
Repeati1005 – 1037ANK 20Add BLAST33
Repeati1043 – 1072ANK 21Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni650 – 759Interaction with RHOD and RAB5ABy similarityAdd BLAST110

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 423NPF3

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1104 – 1164FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG4591 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150873
HOGENOMiHOG000022583
HOVERGENiHBG050501
InParanoidiQ810B6
KOiK20129
OMAiIMARNEY
OrthoDBiEOG091G0ITN
TreeFamiTF351263

Family and domain databases

CDDicd00204 ANK, 7 hits
Gene3Di1.25.40.20, 6 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00023 Ank, 2 hits
PF12796 Ank_2, 4 hits
PF00651 BTB, 1 hit
PF01363 FYVE, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 20 hits
SM00225 BTB, 1 hit
SM00064 FYVE, 1 hit
SUPFAMiSSF48403 SSF48403, 3 hits
SSF54695 SSF54695, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 11 hits
PS50097 BTB, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q810B6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEEEVAKLE KHLMLLRQEY VKLQKKLAET EKRCTLLAAQ ANKENSNESF
60 70 80 90 100
ISRLLAIVAG LYEQEQYSDL KIKVGDRHIS AHKFVLAARS DSWSLANLSS
110 120 130 140 150
TEEIDLSDAN PEVTMTMLRW IYTDELEFRE DDVFLTELMK LANRFQLQLL
160 170 180 190 200
RERCEKGVMS LVNVRNCIRF YQTAEELNAS TLMNYCAEII ASHWDDLRKE
210 220 230 240 250
DFSSLSAQLL YKMIKSKTEY PLHKAIKVER EDVVFLYLIE MDSQLPGKLN
260 270 280 290 300
ETDHNGDLAL DLALSRRLES IATTLVSHKA DVDMVDKNGW SLLHKGIQRG
310 320 330 340 350
DLFASTFLIK NGALVNAATA GAQETPLHLV ALYSPKKYSA DVMSEMAQIA
360 370 380 390 400
EALLQAGANP NMQDSKGRTP LHLSIMARND CVFSQLLQCK QLDLELKDHE
410 420 430 440 450
GSTALWLAVQ YITVSSDQSV NPFEDLPVVN GTSFDENSFA ARLIQRGSNT
460 470 480 490 500
DAPDVMTGNC LLQRAAGAGN EAAALFLATS GAHANHRNKW GETPLHTACR
510 520 530 540 550
HGLANLTAEL LQQGANPNLQ TEEALPVPKE SPVLMSSADS IYLQTPLHMA
560 570 580 590 600
IAYNHPDVVS VILEQKANAL HATNNLQIIP DFSLKDSRDQ TVLGLALWTG
610 620 630 640 650
MHTIAAQLLG SGASINDTMS DGQTLLHMAI QRQDSKSALF LLEHQADINV
660 670 680 690 700
RTQDGETALQ LAIKHQLPLV VDAICTRGAD MSVPDEKGNP PLWLALASNL
710 720 730 740 750
EDIASTLVRH GCDATCWGPG PSGCLQTLLH RAVDENNEST ACFLIRSGCD
760 770 780 790 800
VNSPRQPGTN GEGEEEARDG QTPLHLAASW GLEETVQCLL EFGANVNAQD
810 820 830 840 850
AEGRTPVHVA ISNQHSVIIQ LLISHPNIEL SVRDRQGLTP FACAMTYKNN
860 870 880 890 900
KAAEAILKRE SGAAEQVDNK GRNFLHVAVQ NSDIESVLFL ISVQANVNSR
910 920 930 940 950
VQDASKLTPL HLAVQAGSEI IVRNLLLAGA KVNELTKHRQ TALHLAAQQD
960 970 980 990 1000
LPTICSVLLE NGVDFAAVDE NGNNALHLAV MHGRLNNIRA LLTECTVDAE
1010 1020 1030 1040 1050
AFNLRGQSPL HILGQYGKEN AAAIFDLFLE CMPEYPLDKP DAEGNTVLLL
1060 1070 1080 1090 1100
AYMKGNANLC RAIVRSGVRL GVNNNQGVNI FNYQVATKQL LFRLLDMLSK
1110 1120 1130 1140 1150
EPPWCDGSNC YECTAKFGVT TRKHHCRHCG RLLCHKCSTK EIPIIKFDLN
1160
KPVRVCNICF DVLTLGGVS
Length:1,169
Mass (Da):128,653
Last modified:July 27, 2011 - v2
Checksum:iAAFBB2D66739CA2F
GO
Isoform 2 (identifier: Q810B6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-439: LDLELKDHEG...NGTSFDENSF → YVGRPGEMQS...CVFRPVCKPL
     440-1169: Missing.

Show »
Length:439
Mass (Da):49,771
Checksum:iD2639C8A99B71C70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117 – 118ML → IV in BAA24980 (PubMed:10092534).Curated2
Sequence conflicti117 – 118ML → IV in BAC67211 (Ref. 2) Curated2
Sequence conflicti117 – 118ML → IV in BAC67388 (Ref. 2) Curated2
Sequence conflicti117 – 118ML → IV in BAC67389 (Ref. 2) Curated2
Sequence conflicti439F → L in BAC67211 (Ref. 2) Curated1
Sequence conflicti448S → T in BAC67389 (Ref. 2) Curated1
Sequence conflicti502G → V in BAC67389 (Ref. 2) Curated1
Sequence conflicti526 – 527PV → TL in BAC67211 (Ref. 2) Curated2
Sequence conflicti526P → T in BAC67389 (Ref. 2) Curated1
Sequence conflicti840P → S in BAC67389 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007917392 – 439LDLEL…DENSF → YVGRPGEMQSGCEGGIIRFR AERSRRQHCTLAGRPVHHCV FRPVCKPL in isoform 2. 2 PublicationsAdd BLAST48
Alternative sequenceiVSP_007918440 – 1169Missing in isoform 2. 2 PublicationsAdd BLAST730

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011370 mRNA Translation: BAA24980.2
AB098329 mRNA Translation: BAC67211.1
AB098157 Genomic DNA Translation: BAC67389.1
AB098157 Genomic DNA Translation: BAC67388.1
AL663082, AL808023 Genomic DNA Translation: CAI25188.1
AL808023, AL663082 Genomic DNA Translation: CAI25205.1
BC139231 mRNA Translation: AAI39232.1
AK122479 mRNA Translation: BAC65761.1
CCDSiCCDS24989.1 [Q810B6-1]
PIRiT00253
RefSeqiNP_033801.4, NM_009671.5 [Q810B6-1]
UniGeneiMm.10313

Genome annotation databases

EnsembliENSMUST00000127610; ENSMUSP00000118252; ENSMUSG00000020790 [Q810B6-2]
ENSMUST00000155998; ENSMUSP00000118751; ENSMUSG00000020790 [Q810B6-1]
GeneIDi11736
KEGGimmu:11736
UCSCiuc007jzh.1 mouse [Q810B6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011370 mRNA Translation: BAA24980.2
AB098329 mRNA Translation: BAC67211.1
AB098157 Genomic DNA Translation: BAC67389.1
AB098157 Genomic DNA Translation: BAC67388.1
AL663082, AL808023 Genomic DNA Translation: CAI25188.1
AL808023, AL663082 Genomic DNA Translation: CAI25205.1
BC139231 mRNA Translation: AAI39232.1
AK122479 mRNA Translation: BAC65761.1
CCDSiCCDS24989.1 [Q810B6-1]
PIRiT00253
RefSeqiNP_033801.4, NM_009671.5 [Q810B6-1]
UniGeneiMm.10313

3D structure databases

ProteinModelPortaliQ810B6
SMRiQ810B6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198103, 8 interactors
IntActiQ810B6, 6 interactors
STRINGi10090.ENSMUSP00000118751

PTM databases

iPTMnetiQ810B6
PhosphoSitePlusiQ810B6

Proteomic databases

EPDiQ810B6
PaxDbiQ810B6
PeptideAtlasiQ810B6
PRIDEiQ810B6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000127610; ENSMUSP00000118252; ENSMUSG00000020790 [Q810B6-2]
ENSMUST00000155998; ENSMUSP00000118751; ENSMUSG00000020790 [Q810B6-1]
GeneIDi11736
KEGGimmu:11736
UCSCiuc007jzh.1 mouse [Q810B6-1]

Organism-specific databases

CTDi51479
MGIiMGI:1337008 Ankfy1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG4591 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150873
HOGENOMiHOG000022583
HOVERGENiHBG050501
InParanoidiQ810B6
KOiK20129
OMAiIMARNEY
OrthoDBiEOG091G0ITN
TreeFamiTF351263

Miscellaneous databases

PROiPR:Q810B6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020790 Expressed in 306 organ(s), highest expression level in tracheobronchial tree
CleanExiMM_ANKFY1
GenevisibleiQ810B6 MM

Family and domain databases

CDDicd00204 ANK, 7 hits
Gene3Di1.25.40.20, 6 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00023 Ank, 2 hits
PF12796 Ank_2, 4 hits
PF00651 BTB, 1 hit
PF01363 FYVE, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 20 hits
SM00225 BTB, 1 hit
SM00064 FYVE, 1 hit
SUPFAMiSSF48403 SSF48403, 3 hits
SSF54695 SSF54695, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 11 hits
PS50097 BTB, 1 hit
PS50178 ZF_FYVE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANFY1_MOUSE
AccessioniPrimary (citable) accession number: Q810B6
Secondary accession number(s): B1ATS3
, O54807, Q80TG6, Q80UH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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