UniProtKB - Q80ZJ1 (RAP2A_MOUSE)
Ras-related protein Rap-2a
Rap2a
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.5.2By similarity
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: MGI
- GTPase activity Source: UniProtKB
- GTP binding Source: MGI
- magnesium ion binding Source: MGI
GO - Biological processi
- actin cytoskeleton reorganization Source: UniProtKB
- cellular protein localization Source: UniProtKB
- cellular response to drug Source: MGI
- establishment of protein localization Source: MGI
- microvillus assembly Source: MGI
- negative regulation of cell migration Source: MGI
- positive regulation of protein autophosphorylation Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- protein localization to plasma membrane Source: MGI
- Rap protein signal transduction Source: UniProtKB
- regulation of dendrite morphogenesis Source: UniProtKB
- regulation of JNK cascade Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rap2a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97855, Rap2a |
Subcellular locationi
Endosome
- Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Other locations
- Midbody By similarity
Note: Localizes to midbody at telophase. May also localize to the Golgi and the gelatinase-containing granules of neutrophils.By similarity
Cytosol
- cytosol Source: MGI
Endosome
- recycling endosome Source: MGI
- recycling endosome membrane Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 17 | S → N: Loss of affinity for GTP and loss of interaction with RUNDC3A. 1 Publication | 1 | |
Mutagenesisi | 35 | T → A: Loss of interaction with RUNDC3A. 1 Publication | 1 | |
Mutagenesisi | 176 | C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177. 1 Publication | 1 | |
Mutagenesisi | 177 | C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176. 1 Publication | 1 | |
Mutagenesisi | 180 | C → A: Loss of association with membranes. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082688 | 1 – 180 | Ras-related protein Rap-2aAdd BLAST | 180 | |
PropeptideiPRO_0000281337 | 181 – 183 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 176 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 177 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 180 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 180 | S-farnesyl cysteine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, Prenylation, Ubl conjugationProteomic databases
EPDi | Q80ZJ1 |
jPOSTi | Q80ZJ1 |
PaxDbi | Q80ZJ1 |
PeptideAtlasi | Q80ZJ1 |
PRIDEi | Q80ZJ1 |
PTM databases
iPTMneti | Q80ZJ1 |
PhosphoSitePlusi | Q80ZJ1 |
SwissPalmi | Q80ZJ1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000051615, Expressed in primary visual cortex and 285 other tissues |
Genevisiblei | Q80ZJ1, MM |
Interactioni
Subunit structurei
Interacts with PLCE1.
Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3.
Interacts (GTP-bound form preferentially) with MAP4K4.
Interacts with MINK1.
Interacts with cytoskeletal actin (By similarity).
Interacts (GTP-bound form) with RUNDC3A.
Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4.
Interacts with RGS14; the interaction is GTP-dependent.
By similarity3 PublicationsProtein-protein interaction databases
BioGRIDi | 217966, 5 interactors |
IntActi | Q80ZJ1, 3 interactors |
MINTi | Q80ZJ1 |
STRINGi | 10090.ENSMUSP00000056433 |
Miscellaneous databases
RNActi | Q80ZJ1, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector region | 9 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160594 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q80ZJ1 |
OMAi | IEDCFRT |
OrthoDBi | 1353024at2759 |
PhylomeDBi | Q80ZJ1 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYEKVP VILVGNKVDL ESEREVSSNE GRALAEEWGC PFMETSAKSK
160 170 180
TMVDELFAEI VRQMNYAAQP DKDDPCCSAC NIQ
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013381 | 106 – 183 | Missing in isoform 2. 1 PublicationAdd BLAST | 78 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK018024 mRNA Translation: BAB31042.1 AK140318 mRNA Translation: BAE24330.1 BC043066 mRNA Translation: AAH43066.2 BC049084 mRNA Translation: AAH49084.2 BC053003 mRNA Translation: AAH53003.1 |
CCDSi | CCDS27341.1 [Q80ZJ1-1] |
RefSeqi | NP_083795.2, NM_029519.3 [Q80ZJ1-1] |
Genome annotation databases
Ensembli | ENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1] |
GeneIDi | 76108 |
KEGGi | mmu:76108 |
UCSCi | uc007uzx.1, mouse [Q80ZJ1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK018024 mRNA Translation: BAB31042.1 AK140318 mRNA Translation: BAE24330.1 BC043066 mRNA Translation: AAH43066.2 BC049084 mRNA Translation: AAH49084.2 BC053003 mRNA Translation: AAH53003.1 |
CCDSi | CCDS27341.1 [Q80ZJ1-1] |
RefSeqi | NP_083795.2, NM_029519.3 [Q80ZJ1-1] |
3D structure databases
SMRi | Q80ZJ1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 217966, 5 interactors |
IntActi | Q80ZJ1, 3 interactors |
MINTi | Q80ZJ1 |
STRINGi | 10090.ENSMUSP00000056433 |
PTM databases
iPTMneti | Q80ZJ1 |
PhosphoSitePlusi | Q80ZJ1 |
SwissPalmi | Q80ZJ1 |
Proteomic databases
EPDi | Q80ZJ1 |
jPOSTi | Q80ZJ1 |
PaxDbi | Q80ZJ1 |
PeptideAtlasi | Q80ZJ1 |
PRIDEi | Q80ZJ1 |
Protocols and materials databases
Antibodypediai | 10676, 140 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1] |
GeneIDi | 76108 |
KEGGi | mmu:76108 |
UCSCi | uc007uzx.1, mouse [Q80ZJ1-1] |
Organism-specific databases
CTDi | 5911 |
MGIi | MGI:97855, Rap2a |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160594 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q80ZJ1 |
OMAi | IEDCFRT |
OrthoDBi | 1353024at2759 |
PhylomeDBi | Q80ZJ1 |
TreeFami | TF313014 |
Miscellaneous databases
BioGRID-ORCSi | 76108, 2 hits in 17 CRISPR screens |
ChiTaRSi | Rap2a, mouse |
PROi | PR:Q80ZJ1 |
RNActi | Q80ZJ1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000051615, Expressed in primary visual cortex and 285 other tissues |
Genevisiblei | Q80ZJ1, MM |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP2A_MOUSE | |
Accessioni | Q80ZJ1Primary (citable) accession number: Q80ZJ1 Secondary accession number(s): Q3USK1 Q9D3D5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families