Rap2a

Functionⁱ

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.3 Publications

Miscellaneous

Transgenic mice expressing a constitutively active form of Rap2a display impaired spatial learning and defective extinction of contextual fear.

Activity regulationⁱ

Activated by the guanine nucleotide-exchange factors RAPGEF3 and RAPGEF4 in a cAMP-dependent manner. Nucleotide exchange is also specifically stimulated by RAPGEF5, RASGEF1A and RASGEF1B (By similarity).By similarity

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	10 – 17	GTPBy similarity	8
Nucleotide bindingⁱ	57 – 61	GTPBy similarity	5
Nucleotide bindingⁱ	116 – 119	GTPBy similarity	4

GO - Molecular functionⁱ

GDP binding Source: MGI
GTPase activity Source: UniProtKB
GTP binding Source: MGI
magnesium ion binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cytoskeleton reorganization Source: UniProtKB
cellular protein localization Source: UniProtKB
cellular response to drug Source: MGI
establishment of protein localization
Source: MGI
Inferred from sequence orthologyⁱ
- 19061864
microvillus assembly Source: MGI
negative regulation of cell migration
Source: MGI
Inferred from direct assayⁱ
- 19061864
positive regulation of protein autophosphorylation
Source: MGI
Inferred from direct assayⁱ
- 19061864
positive regulation of protein phosphorylation Source: MGI
protein localization to plasma membrane Source: MGI
Rap protein signal transduction
Source: UniProtKB
Inferred from direct assayⁱ
- 19061864
regulation of dendrite morphogenesis Source: UniProtKB
regulation of JNK cascade Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	GTP-binding, Nucleotide-binding

Ligand

GTP-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ras-related protein Rap-2a
Gene namesⁱ	Name:Rap2a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 14

Organism-specific databases

MGIⁱ	MGI:97855 Rap2a

Keywords - Cellular componentⁱ

Endosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	17	S → N: Loss of affinity for GTP and loss of interaction with RUNDC3A. 1 Publication	1
Mutagenesisⁱ	35	T → A: Loss of interaction with RUNDC3A. 1 Publication	1
Mutagenesisⁱ	176	C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177. 1 Publication	1
Mutagenesisⁱ	177	C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176. 1 Publication	1
Mutagenesisⁱ	180	C → A: Loss of association with membranes. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082688	1 – 180	Ras-related protein Rap-2aAdd BLAST		180
PropeptideⁱPRO_0000281337	181 – 183	Removed in mature form1 Publication		3

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	176	S-palmitoyl cysteine1 Publication	1
Lipidationⁱ	177	S-palmitoyl cysteine1 Publication	1
Modified residueⁱ	180	Cysteine methyl ester1 Publication	1
Lipidationⁱ	180	S-farnesyl cysteine1 Publication	1

Post-translational modificationⁱ

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.1 Publication

Keywords - PTMⁱ

Lipoprotein, Methylation, Palmitate, Prenylation, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q80ZJ1
PeptideAtlas More...PeptideAtlasⁱ	Q80ZJ1
PRIDEⁱ	Q80ZJ1

PTM databases

iPTMnetⁱ	Q80ZJ1
PhosphoSitePlusⁱ	Q80ZJ1
SwissPalmⁱ	Q80ZJ1

Expressionⁱ

Tissue specificityⁱ

Expressed in granular layer of the cerebellum, forebrain, striatum, layer V of the cortex, olfactory cortex, tubercules, subthalamic and hippocampus, particularly in the CA2 region, to a lesser extent in the CA1 region and the external layer of the dentate gyrus. Expressed in neurons.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000051615 Expressed in 271 organ(s), highest expression level in Ammon's horn
CleanExⁱ	MM_RAP2A
Genevisibleⁱ	Q80ZJ1 MM

Interactionⁱ

Subunit structureⁱ

Interacts with PLCE1. Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3. Interacts (GTP-bound form preferentially) with MAP4K4. Interacts with MINK1. Interacts with cytoskeletal actin (By similarity). Interacts (GTP-bound form) with RUNDC3A. Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4. Interacts with RGS14; the interaction is GTP-dependent.By similarity3 Publications

Protein-protein interaction databases

BioGridⁱ	217966, 5 interactors
IntActⁱ	Q80ZJ1, 3 interactors
Molecular INTeraction database More...MINTⁱ	Q80ZJ1
STRINGⁱ	10090.ENSMUSP00000056433

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q80ZJ1
SMRⁱ	Q80ZJ1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	32 – 40	Effector region		9

Domainⁱ

The effector domain mediates the interaction with RUNDC3A.1 Publication

Sequence similaritiesⁱ

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0395 Eukaryota COG1100 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00860000133678
HOGENOMⁱ	HOG000233973
HOVERGENⁱ	HBG009351
InParanoidⁱ	Q80ZJ1
KEGG Orthology (KO) More...KOⁱ	K07837
OMAⁱ	ENLLYWK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OOG
PhylomeDBⁱ	Q80ZJ1
TreeFamⁱ	TF313014

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR001806 Small_GTPase IPR020849 Small_GTPase_Ras-type
PANTHERⁱ	PTHR24070 PTHR24070, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00071 Ras, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51421 RAS, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: Q80ZJ1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP 
        60         70         80         90        100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI 
       110        120        130        140        150
IRVKRYEKVP VILVGNKVDL ESEREVSSNE GRALAEEWGC PFMETSAKSK 
       160        170        180 
TMVDELFAEI VRQMNYAAQP DKDDPCCSAC NIQ

Length:183

Mass (Da):20,642

Last modified:December 21, 2004 - v2

Checksum:ⁱA46054762765E431

GO

Isoform 2 (identifier: Q80ZJ1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
106-183: Missing.

« Hide

        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP 
        60         70         80         90        100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI 

IRVKR

Show »

Length:105

Mass (Da):11,945

Checksum:ⁱAB9A25820AFB5A0B

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_013381	106 – 183	Missing in isoform 2. 1 PublicationAdd BLAST		78

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK018024 mRNA Translation: BAB31042.1 AK140318 mRNA Translation: BAE24330.1 BC043066 mRNA Translation: AAH43066.2 BC049084 mRNA Translation: AAH49084.2 BC053003 mRNA Translation: AAH53003.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27341.1 [Q80ZJ1-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_083795.2, NM_029519.3 [Q80ZJ1-1]
UniGeneⁱ	Mm.261448

Genome annotation databases

Ensemblⁱ	ENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1]
GeneIDⁱ	76108
KEGGⁱ	mmu:76108
UCSC genome browser More...UCSCⁱ	uc007uzx.1 mouse [Q80ZJ1-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q80ZJ1	Ras-related protein Rap-2a		PONAB		183	UniRef100_Q80ZJ1
Ras-related protein Rap-2a		MACMU		183
RAP2A isoform 1		PANTR		183
Ras-related protein Rap-2a		CALJA		183
Uncharacterized protein		PROCO		183
+25
Q80ZJ1-2	RAP2A, member of RAS oncogene family		MACMU		105	UniRef100_A0A1D5QGB4
Uncharacterized protein		GAMAF		282
ras-related protein Rap-2a		LIPVE		105
Uncharacterized protein		MANLE		105
RCG36947		RAT		105
+3

Protein	Similar proteins		Species	Score	Length	Source
Q80ZJ1	Ras-related protein Rap-2a		PONAB		183	UniRef90_Q80ZJ1
Ras-related protein Rap-2a		MACMU		183
RAP2A isoform 1		PANTR		183
Ras-related protein Rap-2a		CALJA		183
Uncharacterized protein		PROCO		183
+35
Q80ZJ1-2	RAP2A, member of RAS oncogene family		MACMU		105	UniRef90_A0A1D5QGB4
Uncharacterized protein		GAMAF		282
ras-related protein Rap-2a		LIPVE		105
Uncharacterized protein		MANLE		105
RCG36947		RAT		105
+3

Protein	Similar proteins		Species	Score	Length	Source
Q80ZJ1	Ras-related protein Rap-2a		PONAB		183	UniRef50_Q80ZJ1
Ras-related protein Rap-2a		MACMU		183
RAP2A isoform 1		PANTR		183
Ras-related protein Rap-2a		CALJA		183
Uncharacterized protein		PROCO		183
+147
Q80ZJ1-2	RAP2A, member of RAS oncogene family		MACMU		105	UniRef50_A0A1D5QGB4
Uncharacterized protein		GAMAF		282
ras-related protein Rap-2a		LIPVE		105
Uncharacterized protein		MANLE		105
RCG36947		RAT		105
+3

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK018024 mRNA Translation: BAB31042.1 AK140318 mRNA Translation: BAE24330.1 BC043066 mRNA Translation: AAH43066.2 BC049084 mRNA Translation: AAH49084.2 BC053003 mRNA Translation: AAH53003.1
CCDSⁱ	CCDS27341.1 [Q80ZJ1-1]
RefSeqⁱ	NP_083795.2, NM_029519.3 [Q80ZJ1-1]
UniGeneⁱ	Mm.261448

3D structure databases

ProteinModelPortalⁱ	Q80ZJ1
SMRⁱ	Q80ZJ1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	217966, 5 interactors
IntActⁱ	Q80ZJ1, 3 interactors
MINTⁱ	Q80ZJ1
STRINGⁱ	10090.ENSMUSP00000056433

PTM databases

iPTMnetⁱ	Q80ZJ1
PhosphoSitePlusⁱ	Q80ZJ1
SwissPalmⁱ	Q80ZJ1

Proteomic databases

PaxDbⁱ	Q80ZJ1
PeptideAtlasⁱ	Q80ZJ1
PRIDEⁱ	Q80ZJ1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1]
GeneIDⁱ	76108
KEGGⁱ	mmu:76108
UCSCⁱ	uc007uzx.1 mouse [Q80ZJ1-1]

Organism-specific databases

CTDⁱ	5911
MGIⁱ	MGI:97855 Rap2a

Phylogenomic databases

eggNOGⁱ	KOG0395 Eukaryota COG1100 LUCA
GeneTreeⁱ	ENSGT00860000133678
HOGENOMⁱ	HOG000233973
HOVERGENⁱ	HBG009351
InParanoidⁱ	Q80ZJ1
KOⁱ	K07837
OMAⁱ	ENLLYWK
OrthoDBⁱ	EOG091G0OOG
PhylomeDBⁱ	Q80ZJ1
TreeFamⁱ	TF313014

Miscellaneous databases

ChiTaRSⁱ	Rap2a mouse
Protein Ontology More...PROⁱ	PR:Q80ZJ1
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000051615 Expressed in 271 organ(s), highest expression level in Ammon's horn
CleanExⁱ	MM_RAP2A
Genevisibleⁱ	Q80ZJ1 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR001806 Small_GTPase IPR020849 Small_GTPase_Ras-type
PANTHERⁱ	PTHR24070 PTHR24070, 1 hit
Pfamⁱ	View protein in Pfam PF00071 Ras, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51421 RAS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RAP2A_MOUSE
Accessionⁱ	Q80ZJ1Primary (citable) accession number: Q80ZJ1 Secondary accession number(s): Q3USK1 , Q7TSK4, Q810A2, Q9D3D5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
	Last sequence update:	December 21, 2004
	Last modified:	November 7, 2018
	This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q80ZJ1 (RAP2A_MOUSE)

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Ras-related protein Rap-2a

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Other locations

Cytosol

Endosome

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ