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Protein

Ras-related protein Rap-2a

Gene

Rap2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.3 Publications

Miscellaneous

Transgenic mice expressing a constitutively active form of Rap2a display impaired spatial learning and defective extinction of contextual fear.

Activity regulationi

Activated by the guanine nucleotide-exchange factors RAPGEF3 and RAPGEF4 in a cAMP-dependent manner. Nucleotide exchange is also specifically stimulated by RAPGEF5, RASGEF1A and RASGEF1B (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-2a
Gene namesi
Name:Rap2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97855 Rap2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17S → N: Loss of affinity for GTP and loss of interaction with RUNDC3A. 1 Publication1
Mutagenesisi35T → A: Loss of interaction with RUNDC3A. 1 Publication1
Mutagenesisi176C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177. 1 Publication1
Mutagenesisi177C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176. 1 Publication1
Mutagenesisi180C → A: Loss of association with membranes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826881 – 180Ras-related protein Rap-2aAdd BLAST180
PropeptideiPRO_0000281337181 – 183Removed in mature form1 Publication3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi176S-palmitoyl cysteine1 Publication1
Lipidationi177S-palmitoyl cysteine1 Publication1
Modified residuei180Cysteine methyl ester1 Publication1
Lipidationi180S-farnesyl cysteine1 Publication1

Post-translational modificationi

Ubiquitinated; undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A.1 Publication
Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation, Ubl conjugation

Proteomic databases

PaxDbiQ80ZJ1
PeptideAtlasiQ80ZJ1
PRIDEiQ80ZJ1

PTM databases

iPTMnetiQ80ZJ1
PhosphoSitePlusiQ80ZJ1
SwissPalmiQ80ZJ1

Expressioni

Tissue specificityi

Expressed in granular layer of the cerebellum, forebrain, striatum, layer V of the cortex, olfactory cortex, tubercules, subthalamic and hippocampus, particularly in the CA2 region, to a lesser extent in the CA1 region and the external layer of the dentate gyrus. Expressed in neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000051615 Expressed in 271 organ(s), highest expression level in Ammon's horn
CleanExiMM_RAP2A
GenevisibleiQ80ZJ1 MM

Interactioni

Subunit structurei

Interacts with PLCE1. Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3. Interacts (GTP-bound form preferentially) with MAP4K4. Interacts with MINK1. Interacts with cytoskeletal actin (By similarity). Interacts (GTP-bound form) with RUNDC3A. Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4. Interacts with RGS14; the interaction is GTP-dependent.By similarity3 Publications

Protein-protein interaction databases

BioGridi217966, 5 interactors
IntActiQ80ZJ1, 3 interactors
MINTiQ80ZJ1
STRINGi10090.ENSMUSP00000056433

Structurei

3D structure databases

ProteinModelPortaliQ80ZJ1
SMRiQ80ZJ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Domaini

The effector domain mediates the interaction with RUNDC3A.1 Publication

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiQ80ZJ1
KOiK07837
OMAiENLLYWK
OrthoDBiEOG091G0OOG
PhylomeDBiQ80ZJ1
TreeFamiTF313014

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q80ZJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYEKVP VILVGNKVDL ESEREVSSNE GRALAEEWGC PFMETSAKSK
160 170 180
TMVDELFAEI VRQMNYAAQP DKDDPCCSAC NIQ
Length:183
Mass (Da):20,642
Last modified:December 21, 2004 - v2
Checksum:iA46054762765E431
GO
Isoform 2 (identifier: Q80ZJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     106-183: Missing.

Show »
Length:105
Mass (Da):11,945
Checksum:iAB9A25820AFB5A0B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013381106 – 183Missing in isoform 2. 1 PublicationAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018024 mRNA Translation: BAB31042.1
AK140318 mRNA Translation: BAE24330.1
BC043066 mRNA Translation: AAH43066.2
BC049084 mRNA Translation: AAH49084.2
BC053003 mRNA Translation: AAH53003.1
CCDSiCCDS27341.1 [Q80ZJ1-1]
RefSeqiNP_083795.2, NM_029519.3 [Q80ZJ1-1]
UniGeneiMm.261448

Genome annotation databases

EnsembliENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1]
GeneIDi76108
KEGGimmu:76108
UCSCiuc007uzx.1 mouse [Q80ZJ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018024 mRNA Translation: BAB31042.1
AK140318 mRNA Translation: BAE24330.1
BC043066 mRNA Translation: AAH43066.2
BC049084 mRNA Translation: AAH49084.2
BC053003 mRNA Translation: AAH53003.1
CCDSiCCDS27341.1 [Q80ZJ1-1]
RefSeqiNP_083795.2, NM_029519.3 [Q80ZJ1-1]
UniGeneiMm.261448

3D structure databases

ProteinModelPortaliQ80ZJ1
SMRiQ80ZJ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217966, 5 interactors
IntActiQ80ZJ1, 3 interactors
MINTiQ80ZJ1
STRINGi10090.ENSMUSP00000056433

PTM databases

iPTMnetiQ80ZJ1
PhosphoSitePlusiQ80ZJ1
SwissPalmiQ80ZJ1

Proteomic databases

PaxDbiQ80ZJ1
PeptideAtlasiQ80ZJ1
PRIDEiQ80ZJ1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062117; ENSMUSP00000056433; ENSMUSG00000051615 [Q80ZJ1-1]
GeneIDi76108
KEGGimmu:76108
UCSCiuc007uzx.1 mouse [Q80ZJ1-1]

Organism-specific databases

CTDi5911
MGIiMGI:97855 Rap2a

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiQ80ZJ1
KOiK07837
OMAiENLLYWK
OrthoDBiEOG091G0OOG
PhylomeDBiQ80ZJ1
TreeFamiTF313014

Miscellaneous databases

ChiTaRSiRap2a mouse
PROiPR:Q80ZJ1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051615 Expressed in 271 organ(s), highest expression level in Ammon's horn
CleanExiMM_RAP2A
GenevisibleiQ80ZJ1 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAP2A_MOUSE
AccessioniPrimary (citable) accession number: Q80ZJ1
Secondary accession number(s): Q3USK1
, Q7TSK4, Q810A2, Q9D3D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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