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Protein

Sialic acid-binding Ig-like lectin 10

Gene

Siglec10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid (PubMed:20038598). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, seems to act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules (By similarity). Involved in negative regulation of B-cell antigen receptor signaling and specifically acts on B1 cells to inhibit Ca2+ signaling, cellular expansion and antibody secretion (PubMed:17572677). The inhibition of B cell activation is dependent on PTPN6/SHP-1 (PubMed:23836061). In association with CD24 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (PubMed:19264983). In association with CD24 may regulate the immune repsonse of natural killer (NK) cells (By similarity). Plays a role in the control of autoimmunity (PubMed:20200274). During initiation of adaptive immune responses by CD8-alpha+ dendritic cells inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes. The function seems to implicate recruitment of PTPN6/SHP-1, which dephosphorylates NCF1 of the NADPH oxidase complex consequently promoting phagosomal acidification (PubMed:27548433).By similarity5 Publications
(Microbial infection) During infection by RNA viruses inhibits DDX58/RIG-I signaling in macrophages by promoting its CBL-dependent ubiquitination and degradation via PTPN11/SHP-2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei120Sialic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • cell adhesion Source: UniProtKB-KW
  • hematopoietic progenitor cell differentiation Source: MGI
  • innate immune response Source: UniProtKB-KW
  • negative regulation of calcium-mediated signaling Source: ARUK-UCL
  • negative regulation of immunoglobulin secretion Source: ARUK-UCL
  • negative regulation of inflammatory response to wounding Source: UniProtKB
  • regulation of B cell proliferation Source: ARUK-UCL
  • regulation of immune response Source: ARUK-UCL

Keywordsi

Biological processAdaptive immunity, Cell adhesion, Immunity, Innate immunity
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 10
Short name:
Siglec-10
Alternative name(s):
Sialic acid-binding Ig-like lectin G
Short name:
Siglec-G
Short name:
mSiglec-G
Gene namesi
Name:Siglec10
Synonyms:Siglecg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2443630 Siglecg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 543ExtracellularSequence analysisAdd BLAST526
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 688CytoplasmicSequence analysisAdd BLAST124

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cd22/Siglec10 double-deficient mice develop autoimmune disease, which is not observed in single-deficient mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120R → E: Decreases interaction with membrane IgM (with greater effect in B1a than in B2 cells). 1 Publication1
Mutagenesisi590Y → F: Decreases interaction with DDX58; when associated with F-635, F-659 and F-682. 1 Publication1
Mutagenesisi635Y → F: Decreases interaction with DDX58; when associated with F-590, F-659 and F-682. 1 Publication1
Mutagenesisi659Y → F: Decreases interaction with DDX58; when associated with F-590,F-635 and F-682. 1 Publication1
Mutagenesisi682Y → F: Decreases interaction with DDX58; when associated with F-590, F-635 and F-659. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_501050895318 – 688Sialic acid-binding Ig-like lectin 10Sequence analysisAdd BLAST671

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 172PROSITE-ProRule annotation
Disulfide bondi42 ↔ 102PROSITE-ProRule annotation
Disulfide bondi163 ↔ 214PROSITE-ProRule annotation
Glycosylationi195N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi246N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi271 ↔ 318PROSITE-ProRule annotation
Disulfide bondi375 ↔ 420PROSITE-ProRule annotation
Modified residuei659PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation of Tyr-659 is involved in binding to PTPN6.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ80ZE3
PaxDbiQ80ZE3
PRIDEiQ80ZE3

PTM databases

iPTMnetiQ80ZE3
PhosphoSitePlusiQ80ZE3

Expressioni

Tissue specificityi

Expressed in B cells with high levels in pre-B cells and B1a cells of the peritoneal cavity.1 Publication

Gene expression databases

BgeeiENSMUSG00000030468 Expressed in 51 organ(s), highest expression level in mesenteric lymph node

Interactioni

Subunit structurei

Interacts with PTPN6/SHP-1 upon phosphorylation. Interacts with NCF1 (PubMed:27548433). Interacts with CD24; the probable CD24:SIGLEC10 complex is proposed to inhibit HGMB1-mediated tissue damage immune response. Interacts with HMGB1; the interaction is dependent on CD24 (PubMed:19264983). Associates with membrane IgM on the B cell surface (PubMed:24790146). Interacts with DDX58, CBL and PTPN11 (PubMed:23374343).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ddx58Q6Q8997EBI-6841023,EBI-6841237

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ80ZE3, 5 interactors
STRINGi10090.ENSMUSP00000005592

Structurei

3D structure databases

ProteinModelPortaliQ80ZE3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 138Ig-like V-typeAdd BLAST113
Domaini145 – 228Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST84
Domaini250 – 334Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST85
Domaini339 – 436Ig-like C2-type 3PROSITE-ProRule annotationAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi588 – 593ITIM motif 1By similarity6
Motifi657 – 662ITIM motif 2By similarity6

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJT6 Eukaryota
ENOG410YKZU LUCA
GeneTreeiENSGT00760000119139
HOGENOMiHOG000236324
HOVERGENiHBG036161
InParanoidiQ80ZE3
KOiK06749
OMAiCRVDFSR
OrthoDBiEOG091G0BMG
PhylomeDBiQ80ZE3
TreeFamiTF332441

Family and domain databases

Gene3Di2.60.40.10, 4 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 4 hits
SM00408 IGc2, 2 hits
SUPFAMiSSF48726 SSF48726, 4 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80ZE3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLLLFLLSF LLDGPQGQME SYFLQVQRIV KAQEGLCIFV PCSFSSPEGK
60 70 80 90 100
WLNRSPLYGY WFKGIRKPSL SFPVATNNKD KVLEWEARGR FQLLGDISKK
110 120 130 140 150
NCSLLIKDVQ WGDSTNYFFR MERGFERFSF KEEFRLQVEA LTQKPDIFIP
160 170 180 190 200
EVLEPGEPVT VVCLFSWTFN QCPAPSFSWM GDAVSFQESR PHTSNYSVLS
210 220 230 240 250
FIPGLQHHDT ELTCQLDFSR MSTQRTVRLR VAYAPRSLAI SIFHDNVSVP
260 270 280 290 300
DLHENPSHLE VQQGQSLRLL CTADSQPPAT LSWVLEDQVL SWSSPVGSRT
310 320 330 340 350
LALELPWVKA GDSGHYTCQA ENRLGSQQHT LDLSVLYPPQ DLRVTVSQAN
360 370 380 390 400
RTVLEILRNA ISLPVLEGQS LCLVCVTYSN PPANVSWAWV TQTLIPIQSS
410 420 430 440 450
EPGVLELPLV QREHEGEFTC AAQNPLGAQR ISLSLSVHYP PQMSSPSCSW
460 470 480 490 500
EAKGLHCNCS SRAWPAPSLR WRLGEGLLEG NSSNASFTVT FSSLGPWVNS
510 520 530 540 550
SLSLLQELGP SLWLSCESWN THGAQTTSVL LLPDKDSATA FSKGAVLGFG
560 570 580 590 600
ITALLALCLI VVIVKTLQKK GTQEEPSRPK LSRGSTILDY INVVPKTRSL
610 620 630 640 650
ARNWKAEPDA PSRSSPLDTH FPKPKKKQKD PHFTYPGCPD PTSSSQVPVS
660 670 680
ENNPEELHYA ALNFSRLRLQ ETQDPQDTYS DYTEVRVH
Length:688
Mass (Da):76,884
Last modified:June 1, 2003 - v1
Checksum:i21A619E1500BFA76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY210400 mRNA Translation: AAO48273.1
AC149091 Genomic DNA No translation available.
CCDSiCCDS52221.1
RefSeqiNP_766488.2, NM_172900.3
UniGeneiMm.24444

Genome annotation databases

EnsembliENSMUST00000005592; ENSMUSP00000005592; ENSMUSG00000030468
GeneIDi243958
KEGGimmu:243958
UCSCiuc009gmo.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY210400 mRNA Translation: AAO48273.1
AC149091 Genomic DNA No translation available.
CCDSiCCDS52221.1
RefSeqiNP_766488.2, NM_172900.3
UniGeneiMm.24444

3D structure databases

ProteinModelPortaliQ80ZE3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80ZE3, 5 interactors
STRINGi10090.ENSMUSP00000005592

PTM databases

iPTMnetiQ80ZE3
PhosphoSitePlusiQ80ZE3

Proteomic databases

MaxQBiQ80ZE3
PaxDbiQ80ZE3
PRIDEiQ80ZE3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005592; ENSMUSP00000005592; ENSMUSG00000030468
GeneIDi243958
KEGGimmu:243958
UCSCiuc009gmo.1 mouse

Organism-specific databases

CTDi243958
MGIiMGI:2443630 Siglecg

Phylogenomic databases

eggNOGiENOG410IJT6 Eukaryota
ENOG410YKZU LUCA
GeneTreeiENSGT00760000119139
HOGENOMiHOG000236324
HOVERGENiHBG036161
InParanoidiQ80ZE3
KOiK06749
OMAiCRVDFSR
OrthoDBiEOG091G0BMG
PhylomeDBiQ80ZE3
TreeFamiTF332441

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Miscellaneous databases

PROiPR:Q80ZE3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030468 Expressed in 51 organ(s), highest expression level in mesenteric lymph node

Family and domain databases

Gene3Di2.60.40.10, 4 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 4 hits
SM00408 IGc2, 2 hits
SUPFAMiSSF48726 SSF48726, 4 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSIG10_MOUSE
AccessioniPrimary (citable) accession number: Q80ZE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2017
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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