UniProtKB - Q80Z10 (ASTN2_MOUSE)
Protein
Astrotactin-2
Gene
Astn2
Organism
Mus musculus (Mouse)
Status
Functioni
Mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. Promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1 (PubMed:20573900). Selectively binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes that contain lipids with a phosphoinositide headgroup (By similarity).By similarity1 Publication
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
GO - Biological processi
- establishment of body hair planar orientation Source: MGI
- negative regulation of protein localization to cell surface Source: MGI
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Astrotactin-2 |
Gene namesi | Name:Astn2 Synonyms:Kiaa0634 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1889277 Astn2 |
Subcellular locationi
Endosome
- Early endosome 1 Publication
- Late endosome 1 Publication
Other locations
- Membrane 1 Publication; Multi-pass membrane protein Curated
- Perikaryon 1 Publication
- cell cortex 1 Publication
- clathrin-coated vesicle 1 Publication
- Cytoplasmic vesicle 1 Publication
Note: Integral membrane protein not detected at the cell membrane. Detected in cytoplasmic vesicles in the cell cortex, close to the anterior pole of migrating neurons. Detected at the base of the leading process in migrating neurons.1 Publication
Endosome
- early endosome Source: UniProtKB-SubCell
- endosome Source: MGI
- late endosome Source: UniProtKB-SubCell
Other locations
- cell cortex Source: UniProtKB-SubCell
- cell pole Source: MGI
- clathrin-coated vesicle Source: UniProtKB-SubCell
- integral component of membrane Source: MGI
- perikaryon Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 52 – 218 | LumenalCuratedAdd BLAST | 167 | |
Transmembranei | 219 – 239 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 240 – 447 | CytoplasmicCuratedAdd BLAST | 208 | |
Transmembranei | 448 – 468 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 469 – 1352 | Lumenal1 PublicationAdd BLAST | 884 |
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 51 | Sequence analysisAdd BLAST | 51 | |
ChainiPRO_0000308253 | 52 – 1352 | Astrotactin-2Add BLAST | 1301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 180 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 527 ↔ 539 | By similarity | ||
Disulfide bondi | 535 ↔ 546 | By similarity | ||
Disulfide bondi | 548 ↔ 562 | By similarity | ||
Disulfide bondi | 668 ↔ 681 | By similarity | ||
Disulfide bondi | 675 ↔ 692 | By similarity | ||
Disulfide bondi | 694 ↔ 707 | By similarity | ||
Disulfide bondi | 716 ↔ 728 | By similarity | ||
Disulfide bondi | 724 ↔ 748 | By similarity | ||
Disulfide bondi | 750 ↔ 763 | By similarity | ||
Glycosylationi | 796 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 838 ↔ 1000 | By similarity | ||
Disulfide bondi | 929 ↔ 990 | By similarity | ||
Disulfide bondi | 996 ↔ 1003 | By similarity | ||
Glycosylationi | 1033 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1049 ↔ 1060 | By similarity | ||
Disulfide bondi | 1062 ↔ 1075 | By similarity | ||
Disulfide bondi | 1149 ↔ 1171 | By similarity | ||
Disulfide bondi | 1203 ↔ 1290 | By similarity | ||
Disulfide bondi | 1311 ↔ 1334 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q80Z10 |
PRIDEi | Q80Z10 |
PTM databases
GlyConnecti | 2137 |
iPTMneti | Q80Z10 |
PhosphoSitePlusi | Q80Z10 |
Expressioni
Tissue specificityi
Detected in cerebellum granule neurons; not detected in astroglia (at protein level). Detected primarily in cerebellum, and at lower levels in brain cortex, olfactory bulb, hindbrain and hippocampus dentate gyrus. Between 6 and 10 days after birth, when granule cell migration occurs in the cerebellum, detected in granule cell precursors in the external germinal layer, the molecular layer, the internal granule layer and in Purkinje neurons. Detected in postmitotic neurons in adult cerebellum.1 Publication
Developmental stagei
Detected at low levels in embryonic brain. Highly expressed 6 and 10 days after birth, when granule cell migration occurs in the cerebellum; expression in cerebellum is considerably higher than in brain cortex. Expressed at lower levels in adult cerebellum.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000028373 Expressed in 71 organ(s), highest expression level in Ammon's horn |
Genevisiblei | Q80Z10 MM |
Interactioni
Subunit structurei
Interacts with ASTN1; the interaction is not calcium-dependent.
1 PublicationProtein-protein interaction databases
IntActi | Q80Z10, 1 interactor |
STRINGi | 10090.ENSMUSP00000065786 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 523 – 563 | EGF-like 1Add BLAST | 41 | |
Domaini | 664 – 708 | EGF-like 2Add BLAST | 45 | |
Domaini | 712 – 764 | EGF-like 3Add BLAST | 53 | |
Domaini | 1079 – 1201 | Fibronectin type-IIIAdd BLAST | 123 |
Domaini
The C-terminal region after the fibronectin type-III domain presents structural similarity to annexin domains and binds calcium ions.By similarity
Sequence similaritiesi
Belongs to the astrotactin family.Curated
Keywords - Domaini
EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410IHIU Eukaryota ENOG4110VB2 LUCA |
GeneTreei | ENSGT00390000003140 |
HOGENOMi | HOG000034112 |
InParanoidi | Q80Z10 |
OMAi | YIKDFPQ |
OrthoDBi | 39300at2759 |
PhylomeDBi | Q80Z10 |
TreeFami | TF332034 |
Family and domain databases
CDDi | cd00063 FN3, 1 hit |
InterProi | View protein in InterPro IPR040685 Annexin-like IPR040510 ASTN_2_hairpin IPR026995 Astrotactin IPR003961 FN3_dom IPR036116 FN3_sf IPR020864 MACPF |
PANTHERi | PTHR16592 PTHR16592, 1 hit |
Pfami | View protein in Pfam PF18411 Annexin_like, 1 hit PF18577 ASTN_2_hairpin, 1 hit PF01823 MACPF, 1 hit |
SMARTi | View protein in SMART SM00457 MACPF, 1 hit |
SUPFAMi | SSF49265 SSF49265, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q80Z10-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAGARRSP GRGLGLRGRP RLGFHPGPPP PPPPPLLLLF LLLLPPPPLL
60 70 80 90 100
AGATAAAASR EPDSPCRLKT VTVSTLPALR ESDIGWSGAR TGAAAGAGAG
110 120 130 140 150
TGAGAGAAAA AASAASPGSA GSAGTAAESR LLLFVRNELP GRIAVQDDLD
160 170 180 190 200
NTELPFFTLE MSGTAADISL VHWRQQWLEN GTLYFHVSMS SSGQLAQATA
210 220 230 240 250
PTLQEPSEIV EEQMHILHIS VMGGLIALLL LLLVFTVALY AQRRWQKRRR
260 270 280 290 300
IPQKSASTEA THEIHYIPSV LLGPQARESF RSSRLQTHNS VIGVPIRETP
310 320 330 340 350
ILDDYDYEEE EEPPRRANHV SREDEFGSQM THALDSLGRP GEEKVEFEKK
360 370 380 390 400
AAAEATQETV ESLMQKFKES FRANTPVEIG QLQPASRSST SAGKRKRRNK
410 420 430 440 450
SRGGISFGRT KGTSGSEADD ETQLTFYTEQ YRSRRRSKGL LKSPVNKTAL
460 470 480 490 500
TLIAVSSCIL AMVCGNQMSC PLTVKVTLHV PEHFIADGSS FVVSEGSYLD
510 520 530 540 550
ISDWLNPAKL SLYYQINATS PWVRDLCGQR TTDACEQLCD PDTGECSCHE
560 570 580 590 600
GYAPDPVHRH LCVRSDWGQS EGPWPYTTLE RGYDLVTGEQ APEKILRSTF
610 620 630 640 650
SLGQGLWLPV SKSFVVPPVE LSINPLASCK TDVLVTEDPA DVREEAMLST
660 670 680 690 700
YFETINDLLS SFGPVRDCSR NNGGCTRNFK CVSDRQVDSS GCVCPEELKP
710 720 730 740 750
MKDGSGCYDH SKGIDCSDGF NGGCEQLCLQ QTLPLPYDTT SSTIFMFCGC
760 770 780 790 800
VEEYKLAPDG KSCLMLSDVC EGPKCLKPDS KFNDTLFGEM LHGYNNRTQH
810 820 830 840 850
VNQGQVFQMT FRENNFIKDF PQLADGLLVI PLPVEEQCRG VLSEPLPDLQ
860 870 880 890 900
LLTGDIRYDE AMGYPMVQQW RVRSNLYRVK LSTITLSAGF TNVLKILTKE
910 920 930 940 950
SSRDELLSFI QHYGSHYIAE ALYGSELTCI IHFPSKKVQQ QLWLQYQKET
960 970 980 990 1000
TELGSKKELK SMPFITYLSG LLTAQMLSDD QLISGVEIRC EEKGRCPSTC
1010 1020 1030 1040 1050
HLCRRPGKEQ LSPTPVLLEI NRVVPLYTLI QDNGTKEAFK NALMSSYWCS
1060 1070 1080 1090 1100
GKGDVIDDWC RCDLSAFDAS GLPNCSPLPQ PVLRLSPTVE PSSTVVSLEW
1110 1120 1130 1140 1150
VDVQPAIGTK VSDYILQHKK VDEYTDTDLY TGEFLSFADD LLSGLGTSCV
1160 1170 1180 1190 1200
AAGRSHGEVP EVSIYSVIFK CLEPDGLYKF TLYAVDTRGR HSELSTVTLR
1210 1220 1230 1240 1250
TACPLVDDNK AEEIADKIYN LYNGYTSGKE QQTAYNTLME VSASMLFRVQ
1260 1270 1280 1290 1300
HHYNSHYEKF GDFVWRSEDE LGPRKAHLIL RRLERVSSHC SSLLRSAYIQ
1310 1320 1330 1340 1350
SRVDTIPYLF CRSEEVRPAG MVWYSILKDT KITCEEKMVS MARNTYGETK
GR
Sequence cautioni
The sequence BAD32265 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058454 | 351 – 402 | Missing in isoform 3. 1 PublicationCuratedAdd BLAST | 52 | |
Alternative sequenceiVSP_028938 | 597 – 600 | Missing in isoform 2. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172987 mRNA Translation: BAD32265.1 Different initiation. AL691438 Genomic DNA No translation available. AL691454 Genomic DNA No translation available. AL691456 Genomic DNA No translation available. AL691474 Genomic DNA No translation available. AL691475 Genomic DNA No translation available. AL713878 Genomic DNA No translation available. AL731829 Genomic DNA No translation available. AL837523 Genomic DNA No translation available. |
CCDSi | CCDS18268.1 [Q80Z10-3] CCDS18269.1 [Q80Z10-1] |
RefSeqi | NP_062387.2, NM_019514.3 [Q80Z10-3] NP_996992.1, NM_207109.2 [Q80Z10-1] XP_006538167.1, XM_006538104.3 [Q80Z10-2] |
Genome annotation databases
Ensembli | ENSMUST00000068214; ENSMUSP00000065786; ENSMUSG00000028373 [Q80Z10-1] ENSMUST00000084496; ENSMUSP00000081540; ENSMUSG00000028373 [Q80Z10-3] |
GeneIDi | 56079 |
KEGGi | mmu:56079 |
UCSCi | uc008thn.1 mouse [Q80Z10-1] uc008tho.1 mouse uc008thp.1 mouse [Q80Z10-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172987 mRNA Translation: BAD32265.1 Different initiation. AL691438 Genomic DNA No translation available. AL691454 Genomic DNA No translation available. AL691456 Genomic DNA No translation available. AL691474 Genomic DNA No translation available. AL691475 Genomic DNA No translation available. AL713878 Genomic DNA No translation available. AL731829 Genomic DNA No translation available. AL837523 Genomic DNA No translation available. |
CCDSi | CCDS18268.1 [Q80Z10-3] CCDS18269.1 [Q80Z10-1] |
RefSeqi | NP_062387.2, NM_019514.3 [Q80Z10-3] NP_996992.1, NM_207109.2 [Q80Z10-1] XP_006538167.1, XM_006538104.3 [Q80Z10-2] |
3D structure databases
SMRi | Q80Z10 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q80Z10, 1 interactor |
STRINGi | 10090.ENSMUSP00000065786 |
PTM databases
GlyConnecti | 2137 |
iPTMneti | Q80Z10 |
PhosphoSitePlusi | Q80Z10 |
Proteomic databases
PaxDbi | Q80Z10 |
PRIDEi | Q80Z10 |
Genome annotation databases
Ensembli | ENSMUST00000068214; ENSMUSP00000065786; ENSMUSG00000028373 [Q80Z10-1] ENSMUST00000084496; ENSMUSP00000081540; ENSMUSG00000028373 [Q80Z10-3] |
GeneIDi | 56079 |
KEGGi | mmu:56079 |
UCSCi | uc008thn.1 mouse [Q80Z10-1] uc008tho.1 mouse uc008thp.1 mouse [Q80Z10-2] |
Organism-specific databases
CTDi | 23245 |
MGIi | MGI:1889277 Astn2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG410IHIU Eukaryota ENOG4110VB2 LUCA |
GeneTreei | ENSGT00390000003140 |
HOGENOMi | HOG000034112 |
InParanoidi | Q80Z10 |
OMAi | YIKDFPQ |
OrthoDBi | 39300at2759 |
PhylomeDBi | Q80Z10 |
TreeFami | TF332034 |
Miscellaneous databases
ChiTaRSi | Astn2 mouse |
PROi | PR:Q80Z10 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028373 Expressed in 71 organ(s), highest expression level in Ammon's horn |
Genevisiblei | Q80Z10 MM |
Family and domain databases
CDDi | cd00063 FN3, 1 hit |
InterProi | View protein in InterPro IPR040685 Annexin-like IPR040510 ASTN_2_hairpin IPR026995 Astrotactin IPR003961 FN3_dom IPR036116 FN3_sf IPR020864 MACPF |
PANTHERi | PTHR16592 PTHR16592, 1 hit |
Pfami | View protein in Pfam PF18411 Annexin_like, 1 hit PF18577 ASTN_2_hairpin, 1 hit PF01823 MACPF, 1 hit |
SMARTi | View protein in SMART SM00457 MACPF, 1 hit |
SUPFAMi | SSF49265 SSF49265, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASTN2_MOUSE | |
Accessioni | Q80Z10Primary (citable) accession number: Q80Z10 Secondary accession number(s): E9Q8T4 Q811Y2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | October 16, 2019 | |
This is version 105 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot