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Protein

Actin filament-associated protein 1

Gene

Afap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1
Alternative name(s):
110 kDa actin filament-associated protein
Short name:
AFAP-110
Gene namesi
Name:Afap1
Synonyms:Kiaa3018
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1917542 Afap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003176591 – 731Actin filament-associated protein 1Add BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei283PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei549PhosphoserineBy similarity1
Modified residuei665PhosphoserineBy similarity1
Modified residuei666PhosphoserineCombined sources1
Modified residuei669PhosphoserineCombined sources1
Modified residuei676PhosphothreonineCombined sources1
Modified residuei680PhosphoserineCombined sources1
Modified residuei688PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80YS6
PaxDbiQ80YS6
PeptideAtlasiQ80YS6
PRIDEiQ80YS6

PTM databases

iPTMnetiQ80YS6
PhosphoSitePlusiQ80YS6

Expressioni

Gene expression databases

BgeeiENSMUSG00000029094 Expressed in 241 organ(s), highest expression level in pineal body
CleanExiMM_AFAP1
ExpressionAtlasiQ80YS6 baseline and differential
GenevisibleiQ80YS6 MM

Interactioni

Subunit structurei

Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers. Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ80YS6, 1 interactor
MINTiQ80YS6
STRINGi10090.ENSMUSP00000067779

Structurei

3D structure databases

ProteinModelPortaliQ80YS6
SMRiQ80YS6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 248PH 1PROSITE-ProRule annotationAdd BLAST97
Domaini348 – 442PH 2PROSITE-ProRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni595 – 638Interaction with F-actinBy similarityAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili558 – 649Sequence analysisAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 73SH3-bindingBy similarity4
Motifi93 – 96SH2-binding 1By similarity4
Motifi452 – 457SH2-binding 2By similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi59 – 105Pro-richAdd BLAST47

Keywords - Domaini

Coiled coil, Repeat, SH3-binding

Phylogenomic databases

eggNOGiENOG410IEU6 Eukaryota
ENOG4111CMN LUCA
GeneTreeiENSGT00390000003998
HOGENOMiHOG000033832
HOVERGENiHBG106875
InParanoidiQ80YS6
KOiK18616
OMAiMCNGKEQ
OrthoDBiEOG091G03NB
PhylomeDBiQ80YS6
TreeFamiTF332622

Family and domain databases

Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR030113 AFAP
IPR029907 AFAP-110
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR14338 PTHR14338, 2 hits
PTHR14338:SF8 PTHR14338:SF8, 2 hits
PfamiView protein in Pfam
PF00169 PH, 2 hits
SMARTiView protein in SMART
SM00233 PH, 2 hits
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q80YS6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEELIVELRL FLELLDHEYL TSTVREKKAV LTNILLRLQS SKGFEVKDHA
60 70 80 90 100
QKAEANNLPA PPQMPLPEIP QPWLPPDSGP PPLPTSSLPE GYYEEAVPLS
110 120 130 140 150
PGKAPEYITS NYDSDAMSSS YESYDEEEED GKGKKTRHQW PSEEASMDLV
160 170 180 190 200
KDAKICAFLL RKKRFGQWTK LLCVIKDTKL LCYKSSKDQQ PQMELPLQGC
210 220 230 240 250
SITYIPRDSK KKKHELKITQ QGTDPLVLAV QSKEQAEQWL KVIKEAYSGC
260 270 280 290 300
SGPVDPECSP PPSTSAPVNK AELEKKLSSE RPSSDGEGGV ENGVTTCNGK
310 320 330 340 350
EQAKRKKPSK SEAKGTVSKV TGKKITKIIG LGKKKPSTDE QTSSAEEDVP
360 370 380 390 400
TCGYLNVLSN SRWRERWCRV KDSKLILHKD RADLKTHLVS IPLRGCEVIP
410 420 430 440 450
GLDSKHPLTF RLLRNGQEVA VLEASSSEDM GRWIGILLAE TGSSTDPGAL
460 470 480 490 500
HYDYIDVEMS ANVIQTAKQT FCFMNRRAVS TSPYLGSLSN GYAHPSGTAL
510 520 530 540 550
HYDDVPCVNG SLKNKKPPAS SNGVPVKGKA PSSQQKKVET AGGVKRTASN
560 570 580 590 600
AEQYKYGKNR VEADAKRLQS KEEELLKRKE ALRNRLAQLR KERKDLRAAI
610 620 630 640 650
EVNAGRKTQA ALEDKLKRLE EECKQREAER VSLELELTEV KESLKKALAG
660 670 680 690 700
GVTLGLAIEP RSGTSSPQSP VFRHRTLENS PISSCDTSDA EGPLPVNSAA
710 720 730
VLKKSQPSSG SSPCRGHVLQ KAKEWELKNG T
Length:731
Mass (Da):80,615
Last modified:June 1, 2003 - v1
Checksum:i58D3D31BED5C2FCD
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q8X9E9Q8X9_MOUSE
Actin filament-associated protein 1
Afap1
627Annotation score:
A0A1D5RLL0A0A1D5RLL0_MOUSE
Actin filament-associated protein 1
Afap1
167Annotation score:

Sequence cautioni

The sequence BAC98293 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43G → R in BAE28086 (PubMed:16141072).Curated1
Sequence conflicti52K → R in BAE28086 (PubMed:16141072).Curated1
Sequence conflicti232S → G in BAC98293 (PubMed:14621295).Curated1
Sequence conflicti249G → D in BAC98293 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129483 mRNA Translation: BAC98293.1 Different initiation.
AK147706 mRNA Translation: BAE28086.1
AK148000 mRNA Translation: BAE28280.1
AK161385 mRNA Translation: BAE36364.1
BC050814 mRNA Translation: AAH50814.1
CCDSiCCDS19236.1
RefSeqiNP_081649.1, NM_027373.2
UniGeneiMm.125167

Genome annotation databases

EnsembliENSMUST00000064571; ENSMUSP00000067779; ENSMUSG00000029094
GeneIDi70292
KEGGimmu:70292
UCSCiuc008xej.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129483 mRNA Translation: BAC98293.1 Different initiation.
AK147706 mRNA Translation: BAE28086.1
AK148000 mRNA Translation: BAE28280.1
AK161385 mRNA Translation: BAE36364.1
BC050814 mRNA Translation: AAH50814.1
CCDSiCCDS19236.1
RefSeqiNP_081649.1, NM_027373.2
UniGeneiMm.125167

3D structure databases

ProteinModelPortaliQ80YS6
SMRiQ80YS6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80YS6, 1 interactor
MINTiQ80YS6
STRINGi10090.ENSMUSP00000067779

PTM databases

iPTMnetiQ80YS6
PhosphoSitePlusiQ80YS6

Proteomic databases

MaxQBiQ80YS6
PaxDbiQ80YS6
PeptideAtlasiQ80YS6
PRIDEiQ80YS6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064571; ENSMUSP00000067779; ENSMUSG00000029094
GeneIDi70292
KEGGimmu:70292
UCSCiuc008xej.1 mouse

Organism-specific databases

CTDi60312
MGIiMGI:1917542 Afap1
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IEU6 Eukaryota
ENOG4111CMN LUCA
GeneTreeiENSGT00390000003998
HOGENOMiHOG000033832
HOVERGENiHBG106875
InParanoidiQ80YS6
KOiK18616
OMAiMCNGKEQ
OrthoDBiEOG091G03NB
PhylomeDBiQ80YS6
TreeFamiTF332622

Miscellaneous databases

ChiTaRSiAfap1 mouse
PROiPR:Q80YS6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029094 Expressed in 241 organ(s), highest expression level in pineal body
CleanExiMM_AFAP1
ExpressionAtlasiQ80YS6 baseline and differential
GenevisibleiQ80YS6 MM

Family and domain databases

Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR030113 AFAP
IPR029907 AFAP-110
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR14338 PTHR14338, 2 hits
PTHR14338:SF8 PTHR14338:SF8, 2 hits
PfamiView protein in Pfam
PF00169 PH, 2 hits
SMARTiView protein in SMART
SM00233 PH, 2 hits
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAFAP1_MOUSE
AccessioniPrimary (citable) accession number: Q80YS6
Secondary accession number(s): Q3UGX1, Q6ZPE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2003
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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