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Protein

Scaffold attachment factor B2

Gene

Safb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI
  • RNA binding Source: UniProtKB-KW
  • sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • regulation of androgen receptor signaling pathway Source: MGI
  • regulation of mRNA processing Source: GO_Central
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • Sertoli cell differentiation Source: MGI

Keywordsi

Molecular functionDNA-binding, Repressor, RNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B2
Short name:
SAF-B2
Gene namesi
Name:Safb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146808 Safb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000819082 – 991Scaffold attachment factor B2Add BLAST990

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei49PhosphoserineBy similarity1
Cross-linki60Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki60Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki89Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei95PhosphoserineCombined sources1
Modified residuei104PhosphoserineBy similarity1
Cross-linki183Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei196PhosphothreonineBy similarity1
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki429Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki435Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki439Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki560Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki568Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki584Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki585Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki620Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki628Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki650Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei658N6-acetyllysine; alternateBy similarity1
Cross-linki658Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei829PhosphoserineBy similarity1
Modified residuei939Omega-N-methylarginineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80YR5
MaxQBiQ80YR5
PaxDbiQ80YR5
PeptideAtlasiQ80YR5
PRIDEiQ80YR5

PTM databases

iPTMnetiQ80YR5
PhosphoSitePlusiQ80YR5

Expressioni

Gene expression databases

BgeeiENSMUSG00000042625 Expressed in 266 organ(s), highest expression level in cerebellum
CleanExiMM_SAFB2
ExpressionAtlasiQ80YR5 baseline and differential
GenevisibleiQ80YR5 MM

Interactioni

Subunit structurei

Interacts with SAFB/SAFB1 and SCAM1. Interacts with SRPK1 and inhibits its activity.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230337, 4 interactors
IntActiQ80YR5, 2 interactors
MINTiQ80YR5
STRINGi10090.ENSMUSP00000074953

Structurei

3D structure databases

ProteinModelPortaliQ80YR5
SMRiQ80YR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 59SAPPROSITE-ProRule annotationAdd BLAST35
Domaini451 – 529RRMPROSITE-ProRule annotationAdd BLAST79

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi755 – 772Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 279Glu-richAdd BLAST80
Compositional biasi661 – 766Glu-richAdd BLAST106
Compositional biasi663 – 836Arg-richAdd BLAST174
Compositional biasi921 – 976Gly-richAdd BLAST56

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111WRB LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
HOVERGENiHBG078408
InParanoidiQ80YR5
OMAiHPYPHFT
OrthoDBiEOG091G0GPT
PhylomeDBiQ80YR5
TreeFamiTF325240

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034782 SAFB2
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF4 PTHR15683:SF4, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q80YR5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEPGTGSGD PAFGPGASES GTRRLSDLRV IDLRAELKKR NLDTGGNKSV
60 70 80 90 100
LMERLRKAFK EEGQEPEEVG ISWGAVSKRA VKRNTKGSKM EEEGSEDNGL
110 120 130 140 150
EEDSRYGQDG VVILQSSQDR DTMDTGVPDG MEAEDLSVPC LGKADTVNQI
160 170 180 190 200
LHAFDDSKEY VAAQLGQLPA QLLKHAVDEE VFKNTLEASV SDLKVTLADE
210 220 230 240 250
EAPMEPENEK ILDILGETCK SEPVKEEGSE LEQPFAQATS SVGPDRKLAE
260 270 280 290 300
EEDLFESCGH PEEEEEEEEE DQEEEQEEEG DLALASSSKS ESPSTRCQWS
310 320 330 340 350
EADAPLAVVK RELADAPGGG GGTRHRRKRK RRRKHQAQAE ALGTGGGAGM
360 370 380 390 400
NCEPVGLEEP VEQSSTAAQL PEATSQELVR APTAALSPEP QDSKEDVKKF
410 420 430 440 450
AFDACNDVPA PPKESSASEG ADQKMSSVKE EQDIKPVIKD EKGRASCSSG
460 470 480 490 500
RNLWVSGLSS STRAADLKSL FSKHGKVIGA KVVTNARSPG ARCYGFVTMS
510 520 530 540 550
TSDEATKCIS HLHRTELHGR MISVEKAKNE PSEKKSSDRR ACDQKEKVPG
560 570 580 590 600
PDRPHPVKIK TEKTVIKKEE KLERKEEKGP EDIKKEKDQD ELTPGAAGHS
610 620 630 640 650
RVTKSGSRGM ERTVVMDKSK GEPVISVKAT SRSKDRSSKS QDRKSEGREK
660 670 680 690 700
RDILSFDKIK EQRERERQRQ REREIRETER RREREQRERE QRLDAFQERR
710 720 730 740 750
EKARLQRERM QLQCQRQRLE RERLERERLE RERMRVERER RKEQQRIMRE
760 770 780 790 800
REELRRQQEQ LRAEQERRAL RRPYDLDARR DDGYWPEGKR AALEDRYRDF
810 820 830 840 850
PRPDHRFHDF DHRDRGHYQE HVIDRRDGSR TRVEERDGQY YPDDQHSHGR
860 870 880 890 900
LLEHHAWDSG DGWHGYSSDK KLNEGQGLPP PPRVSREWAE HSSQLEEQQV
910 920 930 940 950
PVWHSAVDTN MTGHEHIRWR GAERGLAGGP GHGHVAAGRG GMAGQGSFAH
960 970 980 990
GGHSQGYIVP SGRLEGGGMA SQDQGGRVPN PHPHPHFTRR Y
Length:991
Mass (Da):111,838
Last modified:November 14, 2003 - v2
Checksum:i7CA49153C897EB2E
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6WIZ2F6WIZ2_MOUSE
Scaffold attachment factor B2
Safb2
295Annotation score:
F6XJ79F6XJ79_MOUSE
Scaffold attachment factor B2
Safb2
248Annotation score:
F6Z0Y5F6Z0Y5_MOUSE
Scaffold attachment factor B2
Safb2
249Annotation score:
D6RHP9D6RHP9_MOUSE
Scaffold attachment factor B2
Safb2
214Annotation score:
D6RJ78D6RJ78_MOUSE
Scaffold attachment factor B2
Safb2
179Annotation score:
F6VRR0F6VRR0_MOUSE
Scaffold attachment factor B2
Safb2
83Annotation score:
F6R703F6R703_MOUSE
Scaffold attachment factor B2
Safb2
66Annotation score:

Sequence cautioni

The sequence AAH50855 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti861D → E in AAH28827 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC050855 mRNA Translation: AAH50855.1 Different initiation.
BC028827 mRNA Translation: AAH28827.1
CCDSiCCDS28907.2
RefSeqiNP_001025150.2, NM_001029979.2
UniGeneiMm.214973
Mm.482517

Genome annotation databases

EnsembliENSMUST00000075510; ENSMUSP00000074953; ENSMUSG00000042625
GeneIDi224902
KEGGimmu:224902
UCSCiuc008dcg.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC050855 mRNA Translation: AAH50855.1 Different initiation.
BC028827 mRNA Translation: AAH28827.1
CCDSiCCDS28907.2
RefSeqiNP_001025150.2, NM_001029979.2
UniGeneiMm.214973
Mm.482517

3D structure databases

ProteinModelPortaliQ80YR5
SMRiQ80YR5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230337, 4 interactors
IntActiQ80YR5, 2 interactors
MINTiQ80YR5
STRINGi10090.ENSMUSP00000074953

PTM databases

iPTMnetiQ80YR5
PhosphoSitePlusiQ80YR5

Proteomic databases

EPDiQ80YR5
MaxQBiQ80YR5
PaxDbiQ80YR5
PeptideAtlasiQ80YR5
PRIDEiQ80YR5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075510; ENSMUSP00000074953; ENSMUSG00000042625
GeneIDi224902
KEGGimmu:224902
UCSCiuc008dcg.1 mouse

Organism-specific databases

CTDi9667
MGIiMGI:2146808 Safb2

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111WRB LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
HOVERGENiHBG078408
InParanoidiQ80YR5
OMAiHPYPHFT
OrthoDBiEOG091G0GPT
PhylomeDBiQ80YR5
TreeFamiTF325240

Miscellaneous databases

ChiTaRSiSafb2 mouse
PROiPR:Q80YR5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042625 Expressed in 266 organ(s), highest expression level in cerebellum
CleanExiMM_SAFB2
ExpressionAtlasiQ80YR5 baseline and differential
GenevisibleiQ80YR5 MM

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034782 SAFB2
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF4 PTHR15683:SF4, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAFB2_MOUSE
AccessioniPrimary (citable) accession number: Q80YR5
Secondary accession number(s): Q8K153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 7, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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