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Protein

E3 ubiquitin-protein ligase ZNF598

Gene

Znf598

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation. Required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3. Stalling precludes synthesis of a long poly-lysine tail and initiates the RQC pathway to degrade the potentially detrimental aberrant nascent polypeptide. Also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 67RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri185 – 208C2H2-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTranslation regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase ZNF598 (EC:2.3.2.27By similarity)
Alternative name(s):
Zinc finger protein 598Imported
Gene namesi
Name:Znf598By similarity
Synonyms:Zfp598Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2670965 Zfp598

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002505691 – 908E3 ubiquitin-protein ligase ZNF598Add BLAST908

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1
Modified residuei304PhosphotyrosineBy similarity1
Modified residuei433PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80YR4
PeptideAtlasiQ80YR4
PRIDEiQ80YR4

PTM databases

iPTMnetiQ80YR4
PhosphoSitePlusiQ80YR4

Expressioni

Gene expression databases

BgeeiENSMUSG00000041130 Expressed in 294 organ(s), highest expression level in camera-type eye
CleanExiMM_ZFP598
GenevisibleiQ80YR4 MM

Interactioni

Subunit structurei

Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038367

Structurei

3D structure databases

ProteinModelPortaliQ80YR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi645 – 729Pro-richAdd BLAST85
Compositional biasi730 – 741Thr-richAdd BLAST12

Sequence similaritiesi

Belongs to the ZNF598 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 67RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri185 – 208C2H2-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2231 Eukaryota
COG5236 LUCA
GeneTreeiENSGT00390000014178
HOGENOMiHOG000082533
HOVERGENiHBG055813
InParanoidiQ80YR4
KOiK22381
OMAiCVENLKI
OrthoDBiEOG091G022A
PhylomeDBiQ80YR4
TreeFamiTF316196

Family and domain databases

InterProiView protein in InterPro
IPR013087 Znf_C2H2_type
IPR001841 Znf_RING
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q80YR4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAAGAEGR RAALEAVAAP ERGGGSCVLC CGDLEATALG RCDHPVCYRC
60 70 80 90 100
STKMRVLCEQ RYCAVCREEL RQVVFGKKLP AFALIPIHQL QHEKKYDIYF
110 120 130 140 150
ADGKVFALYR QLLQHECPRC PHLPPFSLFG DLEQHMRKQH ELFCCKLCLK
160 170 180 190 200
HLKIFTYERK WYSRKDLARH RMQGDPDDTS HRGHPLCKFC DERYLDNDEL
210 220 230 240 250
LKHLRRDHYF CHFCDSDGAQ DYYSDYAYLR EHFREKHFLC EEGRCSTEQF
260 270 280 290 300
THAFRTEIDL KAHKTACHSR SRAEARQNRQ IDLQFSFAPR HSRRSEGVVS
310 320 330 340 350
GEDYEEVDRY NRQGRAGRAS GRGAQQNRRG SWRYKREEED REVAAAIRAS
360 370 380 390 400
VAAQQQEETQ RVEDREEGSR PKKEEAAARV PEEPRGHRRL PRAQGEGSGS
410 420 430 440 450
KEASANGPVS QEAFPATGPG PVVALSNTLP PPSPELKEED FPSLCASTSS
460 470 480 490 500
CCTAVTPGSV GLALAYPGPP RGKNTFQEED FPALVSSAPK PSSAPSSLIS
510 520 530 540 550
AWNSGCSKKG NLPTPGSQAV VGGSQPPRKA GKGSRGGRKG GPAPVDEEDS
560 570 580 590 600
GGLTVQGLRS VPTTVAVSSL LAPATNQSSA KVGKKKKVGS EKPGATSSPL
610 620 630 640 650
LPPDHTPKPS GAEQVLEAPL SKAEVPVTIV VNGHSEGSAL VRSAPKEPPG
660 670 680 690 700
LPRPLGPLPC PIPQEDFPAL GGPCPPRMPP PPGFSTVVLL KGTPPPPPPP
710 720 730 740 750
PGLVPPISKP PPGFSSLLPS SHSACAPSPT TTTTTTTTTK TPGLAPTPQA
760 770 780 790 800
YLVPENFRER NLQLIQSIKD FLQSDEACFS KFKSHSGEFR QGMISAAQYY
810 820 830 840 850
KSCRDLLGES FQKIFSELLA LLPDTAKQQE LLSAHTDFCS REKPPNSRSK
860 870 880 890 900
RNKKNVWQTS TQQLGLDCCV CPTCQQVLAH GDVSSHQALH AARDDDFPSL

QAIARIIT
Length:908
Mass (Da):99,192
Last modified:June 1, 2003 - v1
Checksum:i528DE75B72EDE40B
GO
Isoform 2 (identifier: Q80YR4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     626-653: Missing.

Note: No experimental confirmation available.
Show »
Length:880
Mass (Da):96,345
Checksum:iCFD751841C179D53
GO
Isoform 3 (identifier: Q80YR4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-335: Missing.

Note: No experimental confirmation available.
Show »
Length:905
Mass (Da):98,745
Checksum:i3A4380670E67943B
GO

Sequence cautioni

The sequence BAD21377 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti600L → M in AAH24690 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020666333 – 335Missing in isoform 3. 1 Publication3
Alternative sequenceiVSP_020667626 – 653Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK131127 mRNA Translation: BAD21377.1 Different initiation.
BC024690 mRNA Translation: AAH24690.1
BC050859 mRNA Translation: AAH50859.1
CCDSiCCDS28490.1 [Q80YR4-1]
RefSeqiNP_001335160.1, NM_001348231.1 [Q80YR4-3]
NP_898972.1, NM_183149.2 [Q80YR4-1]
XP_006524062.1, XM_006523999.1 [Q80YR4-3]
UniGeneiMm.219581

Genome annotation databases

EnsembliENSMUST00000047179; ENSMUSP00000038367; ENSMUSG00000041130 [Q80YR4-1]
GeneIDi213753
KEGGimmu:213753
UCSCiuc008axn.1 mouse [Q80YR4-1]
uc008axo.1 mouse [Q80YR4-2]
uc008axp.1 mouse [Q80YR4-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK131127 mRNA Translation: BAD21377.1 Different initiation.
BC024690 mRNA Translation: AAH24690.1
BC050859 mRNA Translation: AAH50859.1
CCDSiCCDS28490.1 [Q80YR4-1]
RefSeqiNP_001335160.1, NM_001348231.1 [Q80YR4-3]
NP_898972.1, NM_183149.2 [Q80YR4-1]
XP_006524062.1, XM_006523999.1 [Q80YR4-3]
UniGeneiMm.219581

3D structure databases

ProteinModelPortaliQ80YR4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038367

PTM databases

iPTMnetiQ80YR4
PhosphoSitePlusiQ80YR4

Proteomic databases

PaxDbiQ80YR4
PeptideAtlasiQ80YR4
PRIDEiQ80YR4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047179; ENSMUSP00000038367; ENSMUSG00000041130 [Q80YR4-1]
GeneIDi213753
KEGGimmu:213753
UCSCiuc008axn.1 mouse [Q80YR4-1]
uc008axo.1 mouse [Q80YR4-2]
uc008axp.1 mouse [Q80YR4-3]

Organism-specific databases

CTDi213753
MGIiMGI:2670965 Zfp598

Phylogenomic databases

eggNOGiKOG2231 Eukaryota
COG5236 LUCA
GeneTreeiENSGT00390000014178
HOGENOMiHOG000082533
HOVERGENiHBG055813
InParanoidiQ80YR4
KOiK22381
OMAiCVENLKI
OrthoDBiEOG091G022A
PhylomeDBiQ80YR4
TreeFamiTF316196

Miscellaneous databases

ChiTaRSiZfp598 mouse
PROiPR:Q80YR4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041130 Expressed in 294 organ(s), highest expression level in camera-type eye
CleanExiMM_ZFP598
GenevisibleiQ80YR4 MM

Family and domain databases

InterProiView protein in InterPro
IPR013087 Znf_C2H2_type
IPR001841 Znf_RING
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZN598_MOUSE
AccessioniPrimary (citable) accession number: Q80YR4
Secondary accession number(s): Q6KAT0, Q8R3S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2003
Last modified: September 12, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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